UniProt UniProt http://www.uniprot.org TM4SF10 and ADAP interaction in podocytes: role in Fyn activity and nephrin phosphorylation. TM4SF10 and ADAP interaction in podocytes: role in Fyn activity and nephrin phosphorylation. American journal of physiology. Cell physiology 2011 imex curation Azhibekov TA., Wu Z., Padiyar A., Bruggeman LA., Simske JS. Experiments using transfected myc-ADAP (fig 1C) omitted as species for FYB/ADAP cannot be established. Host species for experiments with podocytes according ref 24 (PMID 16204413) jsimske@metrohealth.org 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Azhibekov TA., Wu Z., Padiyar A., Bruggeman LA., Simske JS. Experiments using transfected myc-ADAP (fig 1C) omitted as species for FYB/ADAP cannot be established. Host species for experiments with podocytes according ref 24 (PMID 16204413) jsimske@metrohealth.org Accepted 2016-MAY-12 AT 11:05 BST AT 11:05 BST by ORCHARD 8- to 12.day-old mouse kidney cDNA American journal of physiology. Cell physiology 2011 imex curation TM4SF10 and ADAP interaction in podocytes: role in Fyn activity and nephrin phosphorylation. TM4SF10 and ADAP interaction in podocytes: role in Fyn activity and nephrin phosphorylation. American journal of physiology. Cell physiology 2011 imex curation Azhibekov TA., Wu Z., Padiyar A., Bruggeman LA., Simske JS. Experiments using transfected myc-ADAP (fig 1C) omitted as species for FYB/ADAP cannot be established. Host species for experiments with podocytes according ref 24 (PMID 16204413) jsimske@metrohealth.org 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions mouse-podocyte Mus musculus podocyte renal glomerular podocyte confocal microscopy confocal microscopy immunostaining immunostaining Azhibekov TA., Wu Z., Padiyar A., Bruggeman LA., Simske JS. Experiments using transfected myc-ADAP (fig 1C) omitted as species for FYB/ADAP cannot be established. Host species for experiments with podocytes according ref 24 (PMID 16204413) jsimske@metrohealth.org Accepted 2016-MAY-12 AT 11:05 BST AT 11:05 BST by ORCHARD American journal of physiology. Cell physiology 2011 imex curation TM4SF10 and ADAP interaction in podocytes: role in Fyn activity and nephrin phosphorylation. TM4SF10 and ADAP interaction in podocytes: role in Fyn activity and nephrin phosphorylation. American journal of physiology. Cell physiology 2011 imex curation Azhibekov TA., Wu Z., Padiyar A., Bruggeman LA., Simske JS. Experiments using transfected myc-ADAP (fig 1C) omitted as species for FYB/ADAP cannot be established. Host species for experiments with podocytes according ref 24 (PMID 16204413) jsimske@metrohealth.org 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions mouse-podocyte Mus musculus podocyte renal glomerular podocyte anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Azhibekov TA., Wu Z., Padiyar A., Bruggeman LA., Simske JS. Experiments using transfected myc-ADAP (fig 1C) omitted as species for FYB/ADAP cannot be established. Host species for experiments with podocytes according ref 24 (PMID 16204413) jsimske@metrohealth.org Accepted 2016-MAY-12 AT 11:05 BST AT 11:05 BST by ORCHARD 8- to 12.day-old mouse kidney cDNA American journal of physiology. Cell physiology 2011 imex curation TM4SF10 and ADAP interaction in podocytes: role in Fyn activity and nephrin phosphorylation. TM4SF10 and ADAP interaction in podocytes: role in Fyn activity and nephrin phosphorylation. American journal of physiology. Cell physiology 2011 imex curation Azhibekov TA., Wu Z., Padiyar A., Bruggeman LA., Simske JS. Experiments using transfected myc-ADAP (fig 1C) omitted as species for FYB/ADAP cannot be established. Host species for experiments with podocytes according ref 24 (PMID 16204413) jsimske@metrohealth.org 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions mouse-podocyte Mus musculus podocyte renal glomerular podocyte anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Azhibekov TA., Wu Z., Padiyar A., Bruggeman LA., Simske JS. Experiments using transfected myc-ADAP (fig 1C) omitted as species for FYB/ADAP cannot be established. Host species for experiments with podocytes according ref 24 (PMID 16204413) jsimske@metrohealth.org Accepted 2016-MAY-12 AT 11:05 BST AT 11:05 BST by ORCHARD American journal of physiology. Cell physiology 2011 imex curation fyb1_mouse FYN-binding protein 1 Fyb1 Adhesion and degranulation promoting adaptor protein FYB-120/130 FYN-T-binding protein Fyb SLAP-130 SLP-76-associated phosphoprotein protein protein mouse Mus musculus Mouse MAKFNTGSNPTEEAATSSRPFKVAGQSSPSGIQSRKNLFDNQGNASPPAGPSSMPKFGTTKPPLAAKPTYEEKPEKEPKPPFLKPTGGSPRFGTQPNSVSRDPEVKVGFLKPVSPKPTSLTKEDSKPVVLRPPGNKLHNLNQESDLKTPGPKPGPAPPVPENELKPGFSKVAGAKSKFMPAAQDTDSKPRFPRHTFGQKPSLSTEDSQEENTSKNVPVQKGSPVQLGAKSKGAPFKPPKEDPEDKDHGAPSSPFPGVVLKPAASRGSPGLSKNFEEKKEDRKTDLAKNIFLNKLNQEEPARFPKAPSKLTAGTPWGQSQEKEGDKNSATPKQKALPPLSVLGPPPPKPNRPPNVDLTRFRKADSANSATKSQTPYSTTSLPPPPPTHPASQPPLPASHPAHPPVPSLPPRNIKPPLDLKHPINDENQDGVMHSDGTGNLEEEQESEGETYEDIDSSKERDKKREKEEKKRLELERKEQKEREKKEQELKKKFKLTGPIQVIHHAKACCDVKGGKNELSFKQGEDIEIIRITDNPEGKWLGRTARGSYGYIKTTAVEIDYDSLKRKKNSLNAVPPRLVEDDQDVYDDVAEQDAPNSHGQSGSGGMFPPPPTDDEIYDGIEEEDDDDGSVPQVDEKTNAWSWGILKMLKGKDDRKKSIREKPKVSESDNNEGSSLPSQHKQLDVGEEVYDDVDASDFPPPPAEMSQGMSVGRAKTEEKDPKKLKKQEKEEKDLRKKFKYDGEIRVLYSTKVASSLTSKKWGARDLQIKPGESLEVIQSTDDTKVLCRNEEGKYGYVLRSYLVDNDGEIYDDIADGCIYDND DIP protein O35601 original sequence version: 105 mint Acts as an adapter protein of the FYN and LCP2 signaling cascades in T cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2 6222CC7EF1CA2BBD tmm47_mouse Transmembrane protein 47 Transmembrane 4 superfamily member 10 MNCb-0941 Tmem47 Tm4sf10 protein protein mouse Mus musculus Mouse MASAGSGMEEVRVSVLTPLKLVGLVCIFLALCLDLGAVLSPAWVTADHQYYLSLWESCRKPANLDIWHCESTLGSDWQIATLALLLGGAAIILIAFLVGLISICVGSRRRFYRPVAVMLFAAVVLQVCSLVLYPIKFIETVSLKIYHEFNWGYGLAWGATIFSFGGAILYCLNPKNYEDYY C797B52D502F676C nphn_mouse Nephrin Nphn Renal glomerulus-specific cell adhesion receptor Nphs1 protein protein mouse Mus musculus Mouse MGAKEATVRGPGASPVHRTCHLIPLLLAGMLTTGLAQSPVPTSAPRGFWALSENLTVVEGSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPSVILSILVSPKVLQLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDIIFIQGGRTVEDVSSSVNEGSEEKLFFTEAEARVTPQSSDNGQLLVCEGSNPALATPIKASFTMNILFPPGPPVIDWPGLNEGHVRAGENLELPCIARGGNPPATLQWLKNGKPVSIAWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTILGSTSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTLLVKREDNGLSLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQSIRTGTRVRLVCLAIGGNPEPSLTWLKDSRPVNDPRQSQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNLAKFSCKAGQLSASTQLVVQFPPTNLTILANSSALRPGDALNLTCVSISSNPPVNLSLDKEGERLDDVAAKPQSAPFKGSAASRSVFLRVSSRDHGHRVTCRAHSEALRETVSSFYRLNVLYPPEFLGEQVRAVTVVEQGQALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALKDPTEVNVGGSVDIVCTVDANPILPEMFSWERLGEDEEELNLDDMEKMSKGSTGRLRIRQAKLSQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDHPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPLDLQDPRYTEHKYHQGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSVSPHSVGLEWKPGFDGGLPQRFQIRYEALESPGFLYMDVLPAQATTFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQVSITTPGLDQAPEDTDQPLPTEQPPGPPRLPLLPVLFAVGGLLLLSNASCVGGLLWRRRLRRLAEEISEKTEAGSEEDRIRNEYEESQWTGDRDTRSSTVSTAEVDPHYYSMRDFSPQLPPTLEEVSYRQAFTGIEDEDMAFPGHLYDEVERVYGPPGVWGPLYDEVQMDPYDLRWPEVKYEDPRGIYDQVAADMDAGEPGSLPFELRGHLV DIP protein Q9QZS7 original sequence version: 118 339A670F2AF000A2 fyn_mouse Tyrosine-protein kinase Fyn p59-Fyn Fyn Proto-oncogene c-Fyn protein protein mouse Mus musculus Mouse MGCVQCKDKEAAKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSERAAGLCCRLVVPCHKGMPRLTDLSVKTKDVWEIPRESLQLIKRLGNGQFGEVWMGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQGFLEDYFTATEPQYQPGENL 99558702596DAEE0 fyb-tmem47-1 1 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 13 physical association physical association no figure fyb-tmem47-2 2 5 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 2F fyn-tmem47-1 2 8 unspecified role unspecified role neutral component neutral component 6 tag fluorescence tag visualisation by fluorescence tag fluorescence immunostaining immunostaining unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 2I tmem47-fyb-1 3 5 unspecified role unspecified role prey prey 6 tag fluorescence tag visualisation by fluorescence tag fluorescence western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3A fyn-tmem47-2 4 8 unspecified role unspecified role bait bait 6 tag fluorescence tag visualisation by fluorescence tag fluorescence anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3A nphs1-tmem47-1 2 6 tag fluorescence tag visualisation by fluorescence tag fluorescence immunostaining immunostaining unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 unspecified role unspecified role neutral component neutral component colocalization colocalization 5D fyb-tmem47-3 2 5 unspecified role unspecified role neutral component neutral component 6 tag fluorescence tag visualisation by fluorescence tag fluorescence immunostaining immunostaining unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 2C