HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html The classical swine fever virus N-terminal protease N(pro) binds to cellular HAX-1. The classical swine fever virus N-terminal protease N(pro) binds to cellular HAX-1. The Journal of general virology 2010 imex curation Johns HL., Doceul V., Everett H., Crooke H., Charleston B., Seago J. Virus - Publications including interactions involving viral proteins keep trying to add F1RGL0_PIG but for some reason editor does not pull information The strain of virus used for the Y2H experiments is stated to be Alfort-187. It is unclear which strain transfected N-Pro and GST/GFP tagged N-pro constructs were taken from (see Doceul-2008 reference);but as N-pro was detected in Western blots using an antibody generated using an immunogen sequence with 100% homology to the N-pro from the Alfort-187 strain, and Figure 5A also shows the N-Pro (consensus) sequence which has a 100% match with the the Alfort-187 virus N-Pro sequence, the UniProt ID for N-Pro from this strain has been used throughout. julian.seago@bbsrc.ac.uk 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Johns HL., Doceul V., Everett H., Crooke H., Charleston B., Seago J. Virus - Publications including interactions involving viral proteins keep trying to add F1RGL0_PIG but for some reason editor does not pull information The strain of virus used for the Y2H experiments is stated to be Alfort-187. It is unclear which strain transfected N-Pro and GST/GFP tagged N-pro constructs were taken from (see Doceul-2008 reference);but as N-pro was detected in Western blots using an antibody generated using an immunogen sequence with 100% homology to the N-pro from the Alfort-187 strain, and Figure 5A also shows the N-Pro (consensus) sequence which has a 100% match with the the Alfort-187 virus N-Pro sequence, the UniProt ID for N-Pro from this strain has been used throughout. julian.seago@bbsrc.ac.uk Accepted 2016-NOV-15 AT 10:17 GMT AT 10:17 GMT by MGT The Journal of general virology 2010 imex curation The classical swine fever virus N-terminal protease N(pro) binds to cellular HAX-1. The classical swine fever virus N-terminal protease N(pro) binds to cellular HAX-1. The Journal of general virology 2010 imex curation Johns HL., Doceul V., Everett H., Crooke H., Charleston B., Seago J. Virus - Publications including interactions involving viral proteins keep trying to add F1RGL0_PIG but for some reason editor does not pull information The strain of virus used for the Y2H experiments is stated to be Alfort-187. It is unclear which strain transfected N-Pro and GST/GFP tagged N-pro constructs were taken from (see Doceul-2008 reference);but as N-pro was detected in Western blots using an antibody generated using an immunogen sequence with 100% homology to the N-pro from the Alfort-187 strain, and Figure 5A also shows the N-Pro (consensus) sequence which has a 100% match with the the Alfort-187 virus N-Pro sequence, the UniProt ID for N-Pro from this strain has been used throughout. julian.seago@bbsrc.ac.uk 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation pig-max Max kidney cell line Pig pig-max Max kidney cell line Max kidney cell line from an inbred NIH minipig major histocompatibility complex d/d haplotype confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Johns HL., Doceul V., Everett H., Crooke H., Charleston B., Seago J. Virus - Publications including interactions involving viral proteins It is unclear if results in figure 6 were obtained using Max or PK-15 cells. keep trying to add F1RGL0_PIG but for some reason editor does not pull information The strain of virus used for the Y2H experiments is stated to be Alfort-187. It is unclear which strain transfected N-Pro and GST/GFP tagged N-pro constructs were taken from (see Doceul-2008 reference);but as N-pro was detected in Western blots using an antibody generated using an immunogen sequence with 100% homology to the N-pro from the Alfort-187 strain, and Figure 5A also shows the N-Pro (consensus) sequence which has a 100% match with the the Alfort-187 virus N-Pro sequence, the UniProt ID for N-Pro from this strain has been used throughout. julian.seago@bbsrc.ac.uk Accepted 2016-NOV-15 AT 10:17 GMT AT 10:17 GMT by MGT The Journal of general virology 2010 imex curation The classical swine fever virus N-terminal protease N(pro) binds to cellular HAX-1. The classical swine fever virus N-terminal protease N(pro) binds to cellular HAX-1. The Journal of general virology 2010 imex curation Johns HL., Doceul V., Everett H., Crooke H., Charleston B., Seago J. Virus - Publications including interactions involving viral proteins keep trying to add F1RGL0_PIG but for some reason editor does not pull information The strain of virus used for the Y2H experiments is stated to be Alfort-187. It is unclear which strain transfected N-Pro and GST/GFP tagged N-pro constructs were taken from (see Doceul-2008 reference);but as N-pro was detected in Western blots using an antibody generated using an immunogen sequence with 100% homology to the N-pro from the Alfort-187 strain, and Figure 5A also shows the N-Pro (consensus) sequence which has a 100% match with the the Alfort-187 virus N-Pro sequence, the UniProt ID for N-Pro from this strain has been used throughout. julian.seago@bbsrc.ac.uk 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Johns HL., Doceul V., Everett H., Crooke H., Charleston B., Seago J. Virus - Publications including interactions involving viral proteins keep trying to add F1RGL0_PIG but for some reason editor does not pull information The strain of virus used for the Y2H experiments is stated to be Alfort-187. It is unclear which strain transfected N-Pro and GST/GFP tagged N-pro constructs were taken from (see Doceul-2008 reference);but as N-pro was detected in Western blots using an antibody generated using an immunogen sequence with 100% homology to the N-pro from the Alfort-187 strain, and Figure 5A also shows the N-Pro (consensus) sequence which has a 100% match with the the Alfort-187 virus N-Pro sequence, the UniProt ID for N-Pro from this strain has been used throughout. julian.seago@bbsrc.ac.uk Accepted 2016-NOV-15 AT 10:17 GMT AT 10:17 GMT by MGT Human liver The Journal of general virology 2010 imex curation The classical swine fever virus N-terminal protease N(pro) binds to cellular HAX-1. The classical swine fever virus N-terminal protease N(pro) binds to cellular HAX-1. The Journal of general virology 2010 imex curation Johns HL., Doceul V., Everett H., Crooke H., Charleston B., Seago J. Virus - Publications including interactions involving viral proteins keep trying to add F1RGL0_PIG but for some reason editor does not pull information The strain of virus used for the Y2H experiments is stated to be Alfort-187. It is unclear which strain transfected N-Pro and GST/GFP tagged N-pro constructs were taken from (see Doceul-2008 reference);but as N-pro was detected in Western blots using an antibody generated using an immunogen sequence with 100% homology to the N-pro from the Alfort-187 strain, and Figure 5A also shows the N-Pro (consensus) sequence which has a 100% match with the the Alfort-187 virus N-Pro sequence, the UniProt ID for N-Pro from this strain has been used throughout. julian.seago@bbsrc.ac.uk 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation pig-max Max kidney cell line Pig pig-max Max kidney cell line Max kidney cell line from an inbred NIH minipig major histocompatibility complex d/d haplotype pull down pull down western blot western blot Immuno blot Immuno blot Johns HL., Doceul V., Everett H., Crooke H., Charleston B., Seago J. Virus - Publications including interactions involving viral proteins keep trying to add F1RGL0_PIG but for some reason editor does not pull information The strain of virus used for the Y2H experiments is stated to be Alfort-187. It is unclear which strain transfected N-Pro and GST/GFP tagged N-pro constructs were taken from (see Doceul-2008 reference);but as N-pro was detected in Western blots using an antibody generated using an immunogen sequence with 100% homology to the N-pro from the Alfort-187 strain, and Figure 5A also shows the N-Pro (consensus) sequence which has a 100% match with the the Alfort-187 virus N-Pro sequence, the UniProt ID for N-Pro from this strain has been used throughout. julian.seago@bbsrc.ac.uk Accepted 2016-NOV-15 AT 10:17 GMT AT 10:17 GMT by MGT The Journal of general virology 2010 imex curation hax1_human HCLS1-associated protein X-1 HAX1 HS1BP1 HS1-associating protein X-1 HS1-binding protein 1 protein protein human Homo sapiens Human MSLFDLFRGFFGFPGPRSHRDPFFGGMTRDEDDDEEEEEEGGSWGRGNPRFHSPQHPPEEFGFGFSFSPGGGIRFHDNFGFDDLVRDFNSIFSDMGAWTLPSHPPELPGPESETPGERLREGQTLRDSMLKYPDSHQPRIFGGVLESDARSESPQPAPDWGSQRPFHRFDDVWPMDPHPRTREDNDLDSQVSQEGLGPVLQPQPKSYFKSISVTKITKPDGIVEERRTVVDSEGRTETTVTRHEADSSPRGDPESPRPPALDDAFSILDLFLGRWFRSR DIP protein O00165 original sequence version: 138 87EEF0C46857704B q68871_9flav Autoprotease p20 Capsid protein C Cysteine protease NS2 E(rns) glycoprotein Envelope glycoprotein E1 Envelope glycoprotein E2 Genome polyprotein N-terminal protease NS5B Non-structural protein 2 Non-structural protein 2-3 Non-structural protein 3 Non-structural protein 4A Non-structural protein 4B Non-structural protein 5A RNA-directed RNA polymerase Serine protease NS3 gp33 gp44/48 gp55 Viroporin p7 protein protein classical swine fever virus - alfort/187 classical swine fever virus - alfort/187 MELNHFELLYKTNKQKPMGVEEPVYDATGKPLFGDPSEVHPQSTLKLPHDRGRGNIKTTLKNLPRKGDCRSGNHLGPVSGIYVKPGPVFYQDYMGPVYHRAPLEFFSEAQFCEVTKRIGRVTGSDGRLYHIYVCIDGCILLKLAKRGEPRTLKWIRNFTDCPLWVTSCSDDGASGSKEKKPDRINKGKLKIAPKEHEKDSRTKPPDATIVVEGVKYQVKKKGKVKGKNTQDGLYHNKNKPPESRKKLEKALLAWAVIAIMLYQPVEAENITQWNLSDNGTNGIQHAMYLRGISRSLHGIWPEKICKGVPTYLATDTELKEIQGMMDASEGTNYTCCKLQRHEWNKHGWCNWYNIDPWIQLMNRTQANLAEGPPAKECAVTCRYDKDADVNVVTQARNRPTTLTGCKKGKNFSFAGTVIEGPCNFNVSVEDILYGDHECGSLLQDTALYLVDGMTNTIENARQGAARVTSWLGRQLRIAGKRLEGRSKTWFGAYALSPYCNVTSKIGYIWYTNNCTPACLPKNTKIIGPGKFDTNAEDGKILHEMGGHLSEFLLLSLVVLSDFAPETASALYLILHYVIPQSHEEPEGCDTNQLNLTVELRTEDVIPSSVWNVGKYVCVRPDWWPYETKVALLFEEAGQVVKLALRALRDLTRVWNSASTTAFLICLIKVLRGQIVQGVIWLLLVTGAQGQLACKEDYRYAISSTNEIGLLGAGGLTTTWKEYNHDLQLNDGTVKAICVAGSFKVTALNVVSRRYLASLHKEALPTSVTFELLFDGTNPSTEEMGDDFGFGLCPFDTSPVVKGKYNTTLLNGSAFYLVCPIGWTGVIECTAVSPTTLRTEVVKTFRRDKPFPHRMDCVTTTVENEDLFYCKLGGNWTCVKGEPVVYTGGLVKQCRWCGFDFNEPDGLPHYPIGKCILANETGYRIVDSTDCNRDGVVISTEGSHECLIGNTTVKVHASDERLGPMPCRPKEIVSSAGPVRKTSCTFNYAKTLKNKYYEPRDSYFQQYMLKGEYQYWFDLDVTDRHSDYFAEFVVLVVVALLGGRYILWLIVTYIVLTEQLAAGLPLGQGEVVLIGNLITHTDIEVVVYFLLLYLVMRDEPIKKWILLLFHAMTNNPVKTITVALLMVSGVARGGKIDGGWQRLPETSFDIQLALTVIVVAVMLLAKRDPTTVPLVVTVATLRTAKMTNGLSTDIAIATVSTALLTWTYISDYYRYKTWLQYLISTVTGIFLIRVLKGIGELDLHTPTLPSYRPLFFILVYLISTAVVTRWNLDIAGLLLQCVPTLLMVFTMWADILTLILILPTYELTKLYYLKEVKIGAERGWLWKTNFKRVNDIYEVDQAGEGVYLFPSKQKTSSITGTMLPLIKAILISCISNKWQFIYLLYLIFEVSYYLHKKIIDEIAGGTNFISRLVAALIEANWAFDNEEVRGLKKFFLLSSRVKELIIKHKVRNEVMVHWFGDEEVYGMPKLVGLVKAATLSKNKHCILCTVCEDREWRGETCPKCGRFGPPMTCGMTLADFEEKHYKRIFFREDQSEGPVREEYAGYLQYRARGQLFLRNLPVLATKVKMLLVGNLGTEVGDLEHLGWVLRGPAVCKKVTEHEKCTTSIMDKLTAFFGVMPRGTTPRAPVRFPTSLLKIRRGLETGWAYTHQGGISSVDHVTCGKDLLVCDTMGRTRVVCQSNNKMTDESEYGVKTDSGCPEGARCYVFNPEAVNISGTKGAMVHLQKTGGEFTCVTASGTPAFFDLKNLKGWSGLPIFEASSGRVVGRVKVGKNEDSKPTKLMSGIQTVSKSTTDLTEMVKKITTMNRGEFRQITLATGAGKTTELPRSVIEEIGRHKRVLVLIPLRAAAESVYQYMRQKHPSIAFNLRIGEMKEGDMATGITYASYGYFCQMPQPKLRAAMVEYSFIFLDEYHCATPEQLAIMGKIHRFSENLRVVAMTATPAGTVTTTGQKHPIEEFIAPEVMKGEDLGSEYLDIAGLKIPVEEMKSNMLVFVPTRNMAVETAKKLKAKGYNSGYYYSGEDPSNLRVVTSQSPYVVVATNAIESGVTLPDLDVVVDTGLKCEKRIRLSPKMPFIVTGLKRMAVTIGEQAQRRGRVGRVKPGRYYRSQETPVGSKDYHYDLLQAQRYGIEDGINITKSFREMNYDWSLYEEDSLMITQLEILNNLLISEELPMAVKNIMARTDHPEPIQLAYNSYETQVPVLFPKIKNGEVTDSYDNYTFLNARKLGDDVPPYVYATEDEDLAVELLGLDWPDPGNQGTVEAGRALKQVVGLSTAENALLVALFGYVGYQALSKRHIPVVTDIYSIEDHRLEDTTHLQYAPNAIKTEGKETELKELAQGDVQRCVEAMTNYAREGIQFMKSQALKVKETPTYKETMDTVTDYVKKFMEALTDSKEDIIKYGLWGTHTALYKSICARLGSETAFATLVVKWLAFGGESIADHVKQAATDLVVYYIINRPQFPGDTETQQEGRKFVASLLVSALVTYTYKSWNYNNLSKIVEPALATLPYAATALKLFAPTRLESVVILSTAIYKTYLSIRRGKSDGLLGTGVSAAMEIMSQNPVSVGIAVMLGVGAVAAHNAIEASEQKRTLLMKVFVKNFLDQAATDELVKESPEKIIMALFEAVQTVGNPLRLVYHLYGVFYKGWEAKELAQRTAGRNLFTLIMFEAVELLGVDSEGKIRQLSSNYILELLYKFRDSIKSSVREMAISWAPAPFSCDWTPTDDRIGLPQDNFLQVETKCPCGYKMKAVKNCAGELRLLEEEGSFLCRNKFGRGSRNYRVTKYYDDNLSEIKPVIRMEGHVELYYKGATIKLDFNNSKTILATDKWEVDHSTLVRVLKRHTGAGYHGAYLGEKPNHKHLIERDCATITKDKVCFLKMKRGCAFTYDLSLHNLTRLIELVHKNNLEDKEIPAVTVTTWLAYTFVNEDIGTIKPAFGEKVTPEMQEEITLQPAVVVDTTDVTVTVVGEAPTMTTGETPTAFTSSGSDPKGQQVLKLGVGEGQYPGTNPQRASLHEAIQGADERPSVLILGSDKATSNRVKTAKNVKVYRGRDPLEVRDMMRRGKILVIALSRVDNALLKFVDYKGTFLTRETLEALSLGRPKKKNITKAEAQWLLCLEDQMEELPDWFAAGEPIFLEANIKHDRYHLVGDIATIKEKAKQLGATDSTKISKEVGAKVYSMKLSNWVMQEENKQGNLTPLFEELLQQCPPGGQNKTAHMVSAYQLAQGNWMPTSCHVFMGTISARRTKTHPYEAYVKLRELVEEHKMKTLCPGSSLGKHNEWIIGKIKYQGNLRTKHMLNPGKVAEQLCREGHRRNVYNKTIGSVMTATGIRLEKLPVVRAQTDTTNFHQAIRDKIDKEENLQTPGLHKKLMEVFNALKRPELESSYDAVEWEELERGINRKGAAGFFERKNIGEILDSEKNKVEEIIDNLKKGRNIKYYETAIPKNEKRDVNDDWTSGDFVDEKKPRVIQYPEAKTRLAITKVMYKWVKQKPVVIPGYEGKTPLFQIFDKVKKEWDQFQNPVAVSFDTKAWDTQVTTKDLELIKDIQKYYFKKKWHKFIDTLTMHMSEVPVISADGEVYIRKGQRGSGQPDTSAGNSMLNVLTMVYAFCEATGVPYKSFDRVAKIHVCGDDGFLITERALGEKFASKGVQILYEAGKPQKITEGDKMKVAYQFDDIEFCSHTPIQVRWSDNTSSYMPGRNTTTILAKMATRLDSSGERGTIAYEKAVAFSFLLMYSWNPLIRRICLLVLSTELQVKPGKSTTYYYEGDPISAYKEVIGHNLFDLKRTSFEKLAKLNLSMSVLGAWTRHTSKRLLQDCVNMGVKEGNWLVNADRLVSSKTGNRYIPGEGHTLQGRHYEELVLARKQINNFQGTDRYNLGPIVNMVLRRLRVMMMTLIGRGV f1rgl0_pig HCLS1-associated protein X-1 HAX1 HS1-associating protein X-1 protein protein pig Sus scrofa (Pig) Pig MSLFDLFRGFFGFSRPRSHRDPFFGGMTRDEDEDDEEEEATWSQGSSRFEGPQPPEEFGFGFSFSPGGGMRFHDNFGFDDLVRDFNSIFSEMGAWTLPSRPPASTCHLSAELPGPEIETPGERRQEGQTLRDSMLKYPDSHQPRIFGGGLESDAKTESPKPVPDWDSQRPFHRFDDMWPVTPSSRAREDNDLDSQVSQEGLGPVLQPQPKSYFKSISVTKITKPDGTVEERRTVVDSEGRKETTVTHQEAGDSPRDGPESATPPSLDDAFSILDLFLGRWFRPR 25445FE28362C5B5 npro-hax1-1 3 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait npro region binding region binding-associated region binding region range range range range physical association physical association results npro-hax1-2 1 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait npro region binding region binding-associated region binding region range range range range physical association physical association 1A npro-hax1-3 4 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n_terminal_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain npro region binding region binding-associated region binding region range range range range physical association physical association 1B npro-hax1-4 4 7 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey npro region binding region binding-associated region binding region range range range range physical association physical association 1C-D npro-hax1-6 4 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined npro region binding region binding-associated region binding region range range range range physical association physical association 5C npro-hax1-5 2 6 unspecified role unspecified role neutral component neutral component gfp gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined npro region binding region binding-associated region binding region range range range range 7 immunostaining immunostaining tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component colocalization colocalization 2 f1rgl0_pig-q68871_9flav-1 2 6 unspecified role unspecified role neutral component neutral component gfp gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain npro region binding region binding-associated region binding region range range range range 7 immunostaining immunostaining tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component colocalization colocalization 6 In figure 6 co-localization was seen in both peri-nuclear and cytoplasmic puncta;the authors suggest these puncta may be mitochondrial.