IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A PP4-phosphatase complex dephosphorylates gamma-H2AX generated during DNA replication. A PP4-phosphatase complex dephosphorylates gamma-H2AX generated during DNA replication. Molecular cell 2008 imex curation Chowdhury D., Xu X., Zhong X., Ahmed F., Zhong J., Liao J., Dykxhoorn DM., Weinstock DM., Pfeifer GP., Lieberman J. Results from figure 1C have not been included as it has not been possible to trace the origins of the HA tagged proteins. results from Fig 4D - chromatin fractionation not entered as a crude nuclear fraction is used - little or no purification lieberman@idi.harvard.edu,dipanjan_chowdhury@dfci.harvard.edu,xingzhi_xu@mail.cnu.edu.cn 04-Aug-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chowdhury D., Xu X., Zhong X., Ahmed F., Zhong J., Liao J., Dykxhoorn DM., Weinstock DM., Pfeifer GP., Lieberman J. Results from figure 1C have not been included as it has not been possible to trace the origins of the HA tagged proteins. results from Fig 4D - chromatin fractionation not entered as a crude nuclear fraction is used - little or no purification lieberman@idi.harvard.edu,dipanjan_chowdhury@dfci.harvard.edu,xingzhi_xu@mail.cnu.edu.cn Accepted 2016-JUL-25 AT 11:27 BST AT 11:27 BST by ORCHARD Molecular cell 2008 Only protein-protein interactions imex curation imex curation I have added the interaction with "protein alpha-4". PP2AC:- the immunogen sequence (RGEPHVTRRTPDYFL) is given for this antibody and there is 100% match with a sequence in P62714 (PPP2CB) as well as P67775 (PPP2CA)- so this antibody is not specific. A PP4-phosphatase complex dephosphorylates gamma-H2AX generated during DNA replication. A PP4-phosphatase complex dephosphorylates gamma-H2AX generated during DNA replication. Molecular cell 2008 imex curation Chowdhury D., Xu X., Zhong X., Ahmed F., Zhong J., Liao J., Dykxhoorn DM., Weinstock DM., Pfeifer GP., Lieberman J. Results from figure 1C have not been included as it has not been possible to trace the origins of the HA tagged proteins. results from Fig 4D - chromatin fractionation not entered as a crude nuclear fraction is used - little or no purification lieberman@idi.harvard.edu,dipanjan_chowdhury@dfci.harvard.edu,xingzhi_xu@mail.cnu.edu.cn 04-Aug-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Chowdhury D., Xu X., Zhong X., Ahmed F., Zhong J., Liao J., Dykxhoorn DM., Weinstock DM., Pfeifer GP., Lieberman J. Results from figure 1C have not been included as it has not been possible to trace the origins of the HA tagged proteins. results from Fig 4D - chromatin fractionation not entered as a crude nuclear fraction is used - little or no purification lieberman@idi.harvard.edu,dipanjan_chowdhury@dfci.harvard.edu,xingzhi_xu@mail.cnu.edu.cn Accepted 2016-JUL-25 AT 11:27 BST AT 11:27 BST by ORCHARD Molecular cell 2008 Only protein-protein interactions imex curation imex curation I have added the interaction with "protein alpha-4". PP2AC:- the immunogen sequence (RGEPHVTRRTPDYFL) is given for this antibody and there is 100% match with a sequence in P62714 (PPP2CB) as well as P67775 (PPP2CA)- so this antibody is not specific. pp4c_human Serine/threonine-protein phosphatase 4 catalytic subunit Protein phosphatase X PPP4C PPP4 PPX protein protein human Homo sapiens Human MAEISDLDRQIEQLRRCELIKESEVKALCAKAREILVEESNVQRVDSPVTVCGDIHGQFYDLKELFRVGGDVPETNYLFMGDFVDRGFYSVETFLLLLALKVRYPDRITLIRGNHESRQITQVYGFYDECLRKYGSVTVWRYCTEIFDYLSLSAIIDGKIFCVHGGLSPSIQTLDQIRTIDRKQEVPHDGPMCDLLWSDPEDTTGWGVSPRGAGYLFGSDVVAQFNAANDIDMICRAHQLVMEGYKWHFNETVLTVWSAPNYCYRCGNVAAILELDEHLQKDFIIFEAAPQETRGIPSKKPVADYFL D6FE470A5C6CBCAC p4r3a_human Serine/threonine-protein phosphatase 4 regulatory subunit 3A PPP4R3A KIAA2010 PP4R3A SMEK homolog 1 SMEK1 MSTP033 protein protein human Homo sapiens Human MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDLVDESEEERFDDMSSPGLELPSCELSRLEEIAELVASSLPSPLRREKLALALENEGYIKKLLELFHVCEDLENIEGLHHLYEIIKGIFLLNRTALFEVMFSEECIMDVIGCLEYDPALSQPRKHREFLTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDVSKKLTEQKITSKDILLINLIIEHMICDTDPELGGAVQLMGLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDFQTAQLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGSVPKNTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS EDDB4E1F47197A94 igbp1_human Immunoglobulin-binding protein 1 IGBP1 IBP1 CD79a-binding protein 1 B-cell signal transduction molecule alpha 4 Renal carcinoma antigen NY-REN-16 Protein phosphatase 2/4/6 regulatory subunit protein protein human Homo sapiens Human MAAEDELQLPRLPELFETGRQLLDEVEVATEPAGSRIVQEKVFKGLDLLEKAAEMLSQLDLFSRNEDLEEIASTDLKYLLVPAFQGALTMKQVNPSKRLDHLQRAREHFINYLTQCHCYHVAEFELPKTMNNSAENHTANSSMAYPSLVAMASQRQAKIQRYKQKKELEHRLSAMKSAVESGQADDERVREYYLLHLQRWIDISLEEIESIDQEIKILRERDSSREASTSNSSRQERPPVKPFILTRNMAQAKVFGAGYPSLPTMTVSDWYEQHRKYGALPDQGIAKAAPEEFRKAAQQQEEQEEKEEEDDEQTLHRAREWDDWKDTHPRGYGNRQNMG BB00A116EB45273A pp4r1_human Serine/threonine-protein phosphatase 4 regulatory subunit 1 PPP4R1 MEG1 PP4R1 protein protein human Homo sapiens Human MADLSLLQEDLQEDADGFGVDDYSSESDVIIIPSALDFVSQDEMLTPLGRLDKYAASENIFNRQMVARSLLDTLREVCDDERDCIAVLERISRLADDSEPTVRAELMEQVPHIALFCQENRPSIPYAFSKFLLPIVVRYLADQNNQVRKTSQAALLALLEQELIERFDVETKVCPVLIELTAPDSNDDVKTEAVAIMCKMAPMVGKDITERLILPRFCEMCCDCRMFHVRKVCAANFGDICSVVGQQATEEMLLPRFFQLCSDNVWGVRKACAECFMAVSCATCQEIRRTKLSALFINLISDPSRWVRQAAFQSLGPFISTFANPSSSGQYFKEESKSSEEMSVENKNRTRDQEAPEDVQVRPEDTPSDLSVSNSSVILENTMEDHAAEASGKPLGEISVPLDSSLLCTLSSESHQEAASNENDKKPGNYKSMLRPEVGTTSQDSALLDQELYNSFHFWRTPLPEIDLDIELEQNSGGKPSPEGPEEESEGPVPSSPNITMATRKELEEMIENLEPHIDDPDVKAQVEVLSAALRASSLDAHEETISIEKRSDLQDELDINELPNCKINQEDSVPLISDAVENMDSTLHYIHSDSDLSNNSSFSPDEERRTKVQDVVPQALLDQYLSMTDPSRAQTVDTEIAKHCAYSLPGVALTLGRQNWHCLRETYETLASDMQWKVRRTLAFSIHELAVILGDQLTAADLVPIFNGFLKDLDEVRIGVLKHLHDFLKLLHIDKRREYLYQLQEFLVTDNSRNWRFRAELAEQLILLLELYSPRDVYDYLRPIALNLCADKVSSVRWISYKLVSEMVKKLHAATPPTFGVDLINELVENFGRCPKWSGRQAFVFVCQTVIEDDCLPMDQFAVHLMPHLLTLANDRVPNVRVLLAKTLRQTLLEKDYFLASASCHQEAVEQTIMALQMDRDSDVKYFASIHPASTKISEDAMSTASSTY E1392E6BAD3B58CB p4r3b_human Serine/threonine-protein phosphatase 4 regulatory subunit 3B PPP4R3B KIAA1387 PP4R3B SMEK homolog 2 SMEK2 protein protein human Homo sapiens Human MSDTRRRVKVYTLNEDRQWDDRGTGHVSSTYVEELKGMSLLVRAESDGSLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVEVTQDLIDESEEERFEEMPETSHLIDLPTCELNKLEEIADLVTSVLSSPIRREKLALALENEGYIKKLLQLFQACENLENTEGLHHLYEIIRGILFLNKATLFEVMFSDECIMDVVGCLEYDPALAQPKRHREFLTKTAKFKEVIPITDSELRQKIHQTYRVQYIQDIILPTPSVFEENFLSTLTSFIFFNKVEIVSMLQEDEKFLSEVFAQLTDEATDDDKRRELVNFFKEFCAFSQTLQPQNRDAFFKTLAKLGILPALEIVMGMDDLQVRSAATDIFSYLVEFSPSMVREFVMQEAQQSDDDILLINVVIEQMICDTDPELGGAVQLMGLLRTLIDPENMLATTNKTEKSEFLNFFYNHCMHVLTAPLLTNTSEDKCEKDFFLKHYRYSWSFICTPSHSHSHSTPSSSISQDNIVGSNKNNTICPDNYQTAQLLALILELLTFCVEHHTYHIKNYIMNKDLLRRVLVLMNSKHTFLALCALRFMRRIIGLKDEFYNRYITKGNLFEPVINALLDNGTRYNLLNSAVIELFEFIRVEDIKSLTAHIVENFYKALESIEYVQTFKGLKTKYEQEKDRQNQKLNSVPSILRSNRFRRDAKALEEDEEMWFNEDEEEEGKAVVAPVEKPKPEDDFPDNYEKFMETKKAKESEDKENLPKRTSPGGFKFTFSHSASAANGTNSKSVVAQIPPATSNGSSSKTTNLPTSVTATKGSLVGLVDYPDDEEEDEEEESSPRKRPRLGS CEA2C74C0F2B3D27 pp4r2_human Serine/threonine-protein phosphatase 4 regulatory subunit 2 PPP4R2 SBBI57 protein protein human Homo sapiens Human MDVERLQEALKDFEKRGKKEVCPVLDQFLCHVAKTGETMIQWSQFKGYFIFKLEKVMDDFRTSAPEPRGPPNPNVEYIPFDEMKERILKIVTGFNGIPFTIQRLCELLTDPRRNYTGTDKFLRGVEKNVMVVSCVYPSSEKNNSNSLNRMNGVMFPGNSPSYTERSNINGPGTPRPLNRPKVSLSAPMTTNGLPESTDSKEANLQQNEEKNHSDSSTSESEVSSVSPLKNKHPDEDAVEAEGHEVKRLRFDKEGEVRETASQTTSSEISSVMVGETEASSSSQDKDKDSRCTRQHCTEEDEEEDEEEEEESFMTSREMIPERKNQEKESDDALTVNEETSEENNQMEESDVSQAEKDLLHSEGSENEGPVSSSSSDCRETEELVGSNSSKTGEILSESSMENDDEATEVTDEPMEQD 75298CD34A202F40 ppm1d_human Protein phosphatase 1D Protein phosphatase 2C isoform delta p53-induced protein phosphatase 1 Protein phosphatase magnesium-dependent 1 delta PPM1D WIP1 protein protein human Homo sapiens Human MAGLYSLGVSVFSDQGGRKYMEDVTQIVVEPEPTAEEKPSPRRSLSQPLPPRPSPAALPGGEVSGKGPAVAAREARDPLPDAGASPAPSRCCRRRSSVAFFAVCDGHGGREAAQFAREHLWGFIKKQKGFTSSEPAKVCAAIRKGFLACHLAMWKKLAEWPKTMTGLPSTSGTTASVVIIRGMKMYVAHVGDSGVVLGIQDDPKDDFVRAVEVTQDHKPELPKERERIEGLGGSVMNKSGVNRVVWKRPRLTHNGPVRRSTVIDQIPFLAVARALGDLWSYDFFSGEFVVSPEPDTSVHTLDPQKHKYIILGSDGLWNMIPPQDAISMCQDQEEKKYLMGEHGQSCAKMLVNRALGRWRQRMLRADNTSAIVICISPEVDNQGNFTNEDELYLNLTDSPSYNSQETCVMTPSPCSTPPVKSLEEDPWPRVNSKDHIPALVRSNAFSENFLEVSAEIARENVQGVVIPSKDPEPLEENCAKALTLRIHDSLNNSLPIGLVPTNSTNTVMDQKNLKMSTPGQMKAQEIERTPPTNFKRTLEESNSGPLMKKHRRNGLSRSSGAQPASLPTTSQRKNSVKLTMRRRLRGQKKIGNPLLHQHRKTVCVC DIP protein O15297 original sequence version: 119 E6C5884CF04008B7 h2ax_human Histone H2AX H2AX H2AFX Histone H2A.X protein protein human Homo sapiens Human MSGRGKTGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGHYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGGVTIAQGGVLPNIQAVLLPKKTSATVGPKAPSGGKKATQASQEY DIP protein P16104 original sequence version: 114 DIP protein P16104 original sequence version: 110 DIP protein P16104 original sequence version: 117 DIP protein P16104 original sequence version: 133 DIP protein P16104 original sequence version: 140 DIP protein P16104 original sequence version: 113 DIP protein P16104 original sequence version: 99 D4683775C2E6C3A9 tiprl_human TIP41-like protein Putative MAPK-activating protein PM10 TIPRL Type 2A-interacting protein protein protein human Homo sapiens Human MMIHGFQSSHRDFCFGPWKLTASKTHIMKSADVEKLADELHMPSLPEMMFGDNVLRIQHGSGFGIEFNATDALRCVNNYQGMLKVACAEEWQESRTEGEHSKEVIKPYDWTYTTDYKGTLLGESLKLKVVPTTDHIDTEKLKAREQIKFFEEVLLFEDELHDHGVSSLSVKIRVMPSSFFLLLRFFLRIDGVLIRMNDTRLYHEADKTYMLREYTSRESKISSLMHVPPSLFTEPNEISQYLPIKEAVCEKLIFPERIDPNPADSQKSTQVE 23B730E0BC27DCEA 25w4-1 1 3 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 2A ppp4r1-ppp4c-5 1 7 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey association association 2Ai ppp4r2-ppp4c-3 1 3 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait association association 2Aii ppp4r3a-ppp4c-1 1 7 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey association association 2Aiii ppp4r3b-ppp4c-1 1 11 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey association association 2Aiv tiprl-ppp4c-4 1 9 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association 2Av 35wq5r-1 2 10 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization 4B "CPT":- Camptothecin treatment (CPT is a topoisomerase I inhibitor that induces formation of H2AX foci) did not alter the localisation of PP4R3B or PP4R2 , which remained mainly nuclear. h2afx-ppp4r3b-1 2 10 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 4C "CPT":- Camptothecin treatment (CPT is a topoisomerase I inhibitor that induces formation of H2AX foci) did not alter the localisation of PP4R3B or PP4R2 , which remained mainly nuclear. 25325-1 1 5 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association 2A