UniProt UniProt http://www.uniprot.org Interaction of nephrocystin-4 and RPGRIP1 is disrupted by nephronophthisis or Leber congenital amaurosis-associated mutations. Interaction of nephrocystin-4 and RPGRIP1 is disrupted by nephronophthisis or Leber congenital amaurosis-associated mutations. Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Roepman R., Letteboer SJ., Arts HH., van Beersum SE., Lu X., Krieger E., Ferreira PA., Cremers FP. Rare diseases - Interactions investigated in the context of Rare genetic disease r.roepman@antrg.umcn.nl 17-Oct-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Roepman R., Letteboer SJ., Arts HH., van Beersum SE., Lu X., Krieger E., Ferreira PA., Cremers FP. Rare diseases - Interactions investigated in the context of Rare genetic disease r.roepman@antrg.umcn.nl Accepted 2016-OCT-06 AT 09:48 BST AT 09:48 BST by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2005 Only protein-protein interactions imex curation imex curation Interaction of nephrocystin-4 and RPGRIP1 is disrupted by nephronophthisis or Leber congenital amaurosis-associated mutations. Interaction of nephrocystin-4 and RPGRIP1 is disrupted by nephronophthisis or Leber congenital amaurosis-associated mutations. Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Roepman R., Letteboer SJ., Arts HH., van Beersum SE., Lu X., Krieger E., Ferreira PA., Cremers FP. Rare diseases - Interactions investigated in the context of Rare genetic disease r.roepman@antrg.umcn.nl 17-Oct-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Roepman R., Letteboer SJ., Arts HH., van Beersum SE., Lu X., Krieger E., Ferreira PA., Cremers FP. Rare diseases - Interactions investigated in the context of Rare genetic disease r.roepman@antrg.umcn.nl Accepted 2016-OCT-06 AT 09:48 BST AT 09:48 BST by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2005 Only protein-protein interactions imex curation imex curation Interaction of nephrocystin-4 and RPGRIP1 is disrupted by nephronophthisis or Leber congenital amaurosis-associated mutations. Interaction of nephrocystin-4 and RPGRIP1 is disrupted by nephronophthisis or Leber congenital amaurosis-associated mutations. Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Roepman R., Letteboer SJ., Arts HH., van Beersum SE., Lu X., Krieger E., Ferreira PA., Cremers FP. Rare diseases - Interactions investigated in the context of Rare genetic disease r.roepman@antrg.umcn.nl 17-Oct-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Roepman R., Letteboer SJ., Arts HH., van Beersum SE., Lu X., Krieger E., Ferreira PA., Cremers FP. Rare diseases - Interactions investigated in the context of Rare genetic disease Origin of N-RPGR (Table 1; 3 and 4) according PMID 8817343 (following PMID 10800709 cited by Ref. 2 in supporting information (PMID 10958648)). Origin of RPGRIP1(RID) (Table 1; 3 and 4) according Ref. 2 in supporting information (PMID 10958648); however, the sequence length of bovine RPGRIP1 differs between UniProt and the publication. r.roepman@antrg.umcn.nl Accepted 2016-OCT-06 AT 09:48 BST AT 09:48 BST by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2005 Only protein-protein interactions imex curation imex curation Interaction of nephrocystin-4 and RPGRIP1 is disrupted by nephronophthisis or Leber congenital amaurosis-associated mutations. Interaction of nephrocystin-4 and RPGRIP1 is disrupted by nephronophthisis or Leber congenital amaurosis-associated mutations. Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Roepman R., Letteboer SJ., Arts HH., van Beersum SE., Lu X., Krieger E., Ferreira PA., Cremers FP. Rare diseases - Interactions investigated in the context of Rare genetic disease r.roepman@antrg.umcn.nl 17-Oct-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Roepman R., Letteboer SJ., Arts HH., van Beersum SE., Lu X., Krieger E., Ferreira PA., Cremers FP. Rare diseases - Interactions investigated in the context of Rare genetic disease r.roepman@antrg.umcn.nl Accepted 2016-OCT-06 AT 09:48 BST AT 09:48 BST by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2005 Only protein-protein interactions imex curation imex curation Interaction of nephrocystin-4 and RPGRIP1 is disrupted by nephronophthisis or Leber congenital amaurosis-associated mutations. Interaction of nephrocystin-4 and RPGRIP1 is disrupted by nephronophthisis or Leber congenital amaurosis-associated mutations. Proceedings of the National Academy of Sciences of the United States of America 2005 imex curation Roepman R., Letteboer SJ., Arts HH., van Beersum SE., Lu X., Krieger E., Ferreira PA., Cremers FP. Rare diseases - Interactions investigated in the context of Rare genetic disease r.roepman@antrg.umcn.nl 17-Oct-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Roepman R., Letteboer SJ., Arts HH., van Beersum SE., Lu X., Krieger E., Ferreira PA., Cremers FP. Rare diseases - Interactions investigated in the context of Rare genetic disease Bovine RPGRIP1 binding site according alignment in supplemantary fig 6A and B r.roepman@antrg.umcn.nl Accepted 2016-OCT-06 AT 09:48 BST AT 09:48 BST by ORCHARD bovine retina cDNA Proceedings of the National Academy of Sciences of the United States of America 2005 Only protein-protein interactions imex curation imex curation q92834-2 X-linked retinitis pigmentosa GTPase regulator RPGR RP3 XLRP3 protein protein human Homo sapiens Human MREPEELMPDSGAVFTFGKSKFAENNPGKFWFKNDVPVHLSCGDEHSAVVTGNNKLYMFGSNNWGQLGLGSKSAISKPTCVKALKPEKVKLAACGRNHTLVSTEGGNVYATGGNNEGQLGLGDTEERNTFHVISFFTSEHKIKQLSAGSNTSAALTEDGRLFMWGDNSEGQIGLKNVSNVCVPQQVTIGKPVSWISCGYYHSAFVTTDGELYVFGEPENGKLGLPNQLLGNHRTPQLVSEIPEKVIQVACGGEHTVVLTENAVYTFGLGQFGQLGLGTFLFETSEPKVIENIRDQTISYISCGENHTALITDIGLMYTFGDGRHGKLGLGLENFTNHFIPTLCSNFLRFIVKLVACGGCHMVVFAAPHRGVAKEIEFDEINDTCLSVATFLPYSSLTSGNVLQRTLSARMRRRERERSPDSFSMRRTLPPIEGTLGLSACFLPNSVFPRCSERNLQESVLSEQDLMQPEEPDYLLDEMTKEAEIDNSSTVESLGETTDILNMTHIMSLNSNEKSLKLSPVQKQKKQQTIGELTQDTALTENDDSDEYEEMSEMKEGKACKQHVSQGIFMTQPATTIEAFSDEEVEIPEEKEGAEDSKGNGIEEQEVEANEENVKVHGGRKEKTEILSDDLTDKAEDHEFSKTEELKLEDVDEEINAENVESKKKTVGDDESVPTGYHSKTEGAERTNDDSSAETIEKKEKANLEERAICEYNENPKGYMLDDADSSSLEILENSETTPSKDMKKTKKIFLFKRVPSINQKIVKNNNEPLPEIKSIGDQIILKSDNKDADQNHMSQNHQNIPPTNTERRSKSCTIL 70D84EAD988348D1 rpgr1_bovin X-linked retinitis pigmentosa GTPase regulator-interacting protein 1 RPGRIP1 protein protein bovin Bos taurus (Bovine) Bovine MIPTSKGKNTKTQPPLSRMTRDELEDSLFRLREEHMLVKELFWKQQDEIKRMRTALLRLTASGRGLRAEAAADESSGSPLNGGGTESGGTAPSSTSVPRCPGSSCSSSAWAPLLPAAPSLASTRDTDSSTPPGHRAEKPKRESRDRLSYTAPPTFKEHVTNEKARGEVASEPSELGHLMTSDTMQVEEPPKSPEKMWSKDENFAQRSSLESTQKATELRASIKENIQLIRLKKLLHERNTSLAVTKAQLTEVQAAYETLLHKNQGILGAAHNALLSQVNELRAELKEESKKAVSLKSQMEDVSILQITLKEFQERVEDLEKERKLLNDNYDKLLESMLDSSNQPQWSHELGEQLQQKVSQLQDQLDVEMKEKREILLQLSQEKAQNKDLELEVTSLLQKHKQEVEDLQNISTFSQSPDRQSAPATHPALFQETIQIQPCEPKNQEEKKLSQMLSELQVSHAETTLELEKTRDMLILQRKINVCYQEELEAMMTKADNENKDHEAKLERLNQLLDLKNKRINQLEEQLKDVAYGTRQLPLCLKPLPAHENEDKVDISPWHQSENLFELHIHQAFLTSAALAQAGDTQPTTFCTYSFYDFETHCTPLVVGPQPLYDFTSQYVVEIDSLFLHYLQGASAQLDLHQAIASEHHTLAAGWICFDRVLETVERVHGSATLTGTGGEVFGVLEYWMRLRFPIRSSLQAYNKRKKAQAYLAANVLGAWEAQKDEPRSGTWKNQNELRVEIIRCCGLRSRSLGAQPSPYVMYRFFTFSDHDTTIIPASSNPYFRDLARFPVLVTSDLDQYLRREALSVYVFDDEDSEPGSYLGRVQVPLLPLAQNKSIQGDFNLTDPVGEPNGSVQVHLDWGSCYLPPENFPKPEAQSEEDTRDGLETSIEEEEASFPPQDQMVSIDTPTEAGQYQAKRKPPQVGERKEREHQVAGYSRRKHGRKTGLQGKNRMEYLSHNLLNGNTLQQVKYIEWKFSGLKISADHVLKNQQKEEEMTSSYSAQILKETPHPVNDKEFCEQASEGSEAQTTDSDEIVTPVSQKCPKADSEKMCIEIVSLAFYPEAEVMCDENVEQVYVEYRFYDLPLSETETPVSLRKPRAGEEIYFHFSKVIDLDPVEQKERRQFLFTMLIGEDPEQGHLKFTVVSDPIEEEKKECQEVGYAYLELWPMLVSGRDILEQDLDIVGPEDQATPIGKLKVSLQAAAALQAIYKEMTEDLCS 8E4CE05E3CACB64A e1bjb5_bovin NPHP4 protein protein bovin Bos taurus (Bovine) Bovine MHDWHRIFDQNLLVPPHPQRARQPLKESTAFQCVLKWLDGPLIKQGVLELLSEVGCHLRVSLFDVTYRHFFGRTWRSSPRPAEPLTRQPARIVFNEPLYFHTSLNHPHIVAVVEVVVEGRRRDGTLQTLSCGFGILRIFSHKPESPTSTSQDKRLRLYHGSPRALLHPLLQDPIEQNKHMTLIENCSLQYTLRPHPLLEPVFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPLTCYLDDLFFTLYPSLEKFEEELLELVISDCFREAGSPLDVGTLEILERRLCVGVHNGLGFVQRPQVLVLVPELEVALTRSASFSRKVGSSSKASSGNQALVLRSRLRLPEMVRHPAFAIVFQLEYVFNSPSGADSNAASLSSLSSLACMHMVRWAVWNPSLEASSGQVMLPLQGGIQSNPSHCLVYKVPPASISSEEVKQVESGTVQFQYSLSPEEHPGTPTANASSPKAERRSFRKPPVSPSSPPASPSQGLTAAQDSPMGPGLSLSQLAASPPSPAPRRRSAKPSSQPPGSPVPSLVPGEFPLEGRISHLEADLSQTALALGTSAADHLQELPFTPVHAPIIAGTQTRSSGGKLSRASLALLQSAGFPEILDANKQPAEAVSPTDPVKFSPQKEESDCLQGNEIVLQFLAFSRVPQDEQAAPWPQTVYFTFQFYRFPPETTPRLQLVELADAGKTSSASLSHILAQIQKDGSFDAGSPGFQRKYLVDPGFLRPGEQRWFARYLAMQTLQIDVWDGDSLLLIGSAAVQLKHLLRQGRPAVQVSHELEVVATEYEQDAMVVSGDMTKFGCVRPIDVHTVVKGQLHLTLANVGHLCEQRVRSPSSLPPSRSRVISNDGVGNFMGGSLLTPGGPRLKNVVQAQKLADVDSELAALLFTQPRPGARGPQGINQEGDAVRRRKLERMRAVRLRECGLEPGRRGLGALAQQSLHAQHARDLQVIAAYRERTKAESIASALSTAITTQHTIHATLGAAEFFEFALRNPHNAQHMVTIEIDNPELSLILDGQEWRYFKDMADLHTPLEEDMFHLRGSLAPQLFLRPRETAHIPFKFQTFSVGPWASAQASAELRSEKDMDAGSPYKSSMMPTKHAKVLFRAGSGKPLAVLCLTVELQPHVVDQVFRFHHPELTFLKKAIRLPPWHTLPGAPVGTSSEDPPFHVRCSDPNIICETQSVGPGEPRDVFLKVASGPSPEVKDFFVTIYVDRWLATPVQTWQVHLHSLQRVDVSCITGQRTRLALVLRGTQTVRRVQAFSSHPQELTTDPRGVFVLPPHGVQDLHVSVRPCRPGSRFAHLHLVDVDAHQLVASWLLCLSCRPPLISKAFEIKLPAGSGRGANKRITYTNPYPSRRAYRLHSDHPHLLQFKEDAFQVGGGETYTIGLRFVSRQRAGEEEILIYINDHEDKNEETFCVKVIYQ B6028557C130E612 q96kn7-1 X-linked retinitis pigmentosa GTPase regulator-interacting protein 1 RPGRIP1 protein protein human Homo sapiens Human MSHLVDPTSGDLPVRDIDAIPLVLPASKGKNMKTQPPLSRMNREELEDSFFRLREDHMLVKELSWKQQDEIKRLRTTLLRLTAAGRDLRVAEEAAPLSETARRGQKAGWRQRLSMHQRPQMHRLQGHFHCVGPASPRRAQPRVQVGHRQLHTAGAPVPEKPKRGPRDRLSYTAPPSFKEHATNENRGEVASKPSELVSGSNSIISFSSVISMAKPIGLCMPNSAHIMASNTMQVEEPPKSPEKMWPKDENFEQRSSLECAQKAAELRASIKEKVELIRLKKLLHERNASLVMTKAQLTEVQEAYETLLQKNQGILSAAHEALLKQVNELRAELKEESKKAVSLKSQLEDVSILQMTLKEFQERVEDLEKERKLLNDNYDKLLESMLDSSDSSSQPHWSNELIAEQLQQQVSQLQDQLDAELEDKRKVLLELSREKAQNEDLKLEVTNILQKHKQEVELLQNAATISQPPDRQSEPATHPAVLQENTQIEPSEPKNQEEKKLSQVLNELQVSHAETTLELEKTRDMLILQRKINVCYQEELEAMMTKADNDNRDHKEKLERLTRLLDLKNNRIKQLEGILRSHDLPTSEQLKDVAYGTRPLSLCLETLPAHGDEDKVDISLLHQGENLFELHIHQAFLTSAALAQAGDTQPTTFCTYSFYDFETHCTPLSVGPQPLYDFTSQYVMETDSLFLHYLQEASARLDIHQAMASEHSTLAAGWICFDRVLETVEKVHGLATLIGAGGEEFGVLEYWMRLRFPIKPSLQACNKRKKAQVYLSTDVLGGRKAQEEEFRSESWEPQNELWIEITKCCGLRSRWLGTQPSPYAVYRFFTFSDHDTAIIPASNNPYFRDQARFPVLVTSDLDHYLRREALSIHVFDDEDLEPGSYLGRARVPLLPLAKNESIKGDFNLTDPAEKPNGSIQVQLDWKFPYIPPESFLKPEAQTKGKDTKDSSKISSEEEKASFPSQDQMASPEVPIEAGQYRSKRKPPHGGERKEKEHQVVSYSRRKHGKRIGVQGKNRMEYLSLNILNGNTPEQVNYTEWKFSETNSFIGDGFKNQHEEEEMTLSHSALKQKEPLHPVNDKESSEQGSEVSEAQTTDSDDVIVPPMSQKYPKADSEKMCIEIVSLAFYPEAEVMSDENIKQVYVEYKFYDLPLSETETPVSLRKPRAGEEIHFHFSKVIDLDPQEQQGRRRFLFDMLNGQDPDQGHLKFTVVSDPLDEEKKECEEVGYAYLQLWQILESGRDILEQELDIVSPEDLATPIGRLKVSLQAAAVLHAIYKEMTEDLFS 00AC1C2A0AC82253 q68cz1-1 Protein fantom Nephrocystin-8 RPGR-interacting protein 1-like protein RPGRIP1L FTM KIAA1005 NPHP8 protein protein human Homo sapiens Human MSGPTDETAGDLPVKDTGLNLFGMGGLQETSTTRTMKSRQAVSRVSREELEDRFLRLHDENILLKQHARKQEDKIKRMATKLIRLVNDKKRYERVGGGPKRLGRDVEMEEMIEQLQEKVHELEKQNETLKNRLISAKQQLQTQGYRQTPYNNVQSRINTGRRKANENAGLQECPRKGIKFQDADVAETPHPMFTKYGNSLLEEARGEIRNLENVIQSQRGQIEELEHLAEILKTQLRRKENEIELSLLQLREQQATDQRSNIRDNVEMIKLHKQLVEKSNALSAMEGKFIQLQEKQRTLRISHDALMANGDELNMQLKEQRLKCCSLEKQLHSMKFSERRIEELQDRINDLEKERELLKENYDKLYDSAFSAAHEEQWKLKEQQLKVQIAQLETALKSDLTDKTEILDRLKTERDQNEKLVQENRELQLQYLEQKQQLDELKKRIKLYNQENDINADELSEALLLIKAQKEQKNGDLSFLVKVDSEINKDLERSMRELQATHAETVQELEKTRNMLIMQHKINKDYQMEVEAVTRKMENLQQDYELKVEQYVHLLDIRAARIHKLEAQLKDIAYGTKQYKFKPEIMPDDSVDEFDETIHLERGENLFEIHINKVTFSSEVLQASGDKEPVTFCTYAFYDFELQTTPVVRGLHPEYNFTSQYLVHVNDLFLQYIQKNTITLEVHQAYSTEYETIAACQLKFHEILEKSGRIFCTASLIGTKGDIPNFGTVEYWFRLRVPMDQAIRLYRERAKALGYITSNFKGPEHMQSLSQQAPKTAQLSSTDSTDGNLNELHITIRCCNHLQSRASHLQPHPYVVYKFFDFADHDTAIIPSSNDPQFDDHMYFPVPMNMDLDRYLKSESLSFYVFDDSDTQENIYIGKVNVPLISLAHDRCISGIFELTDHQKHPAGTIHVILKWKFAYLPPSGSITTEDLGNFIRSEEPEVVQRLPPASSVSTLVLAPRPKPRQRLTPVDKKVSFVDIMPHQSDETSPPPEDRKEISPEVEHIPEIEINMLTVPHVPKVSQEGSVDEVKENTEKMQQGKDDVSLLSEGQLAEQSLASSEDETEITEDLEPEVEEDMSASDSDDCIIPGPISKNIKQSLALSPGLGCSSAISAHCNFRLPGSSDFPASASQVDGITGACHHTQPSEKIRIEIIALSLNDSQVTMDDTIQRLFVECRFYSLPAEETPVSLPKPKSGQWVYYNYSNVIYVDKENNKAKRDILKAILQKQEMPNRSLRFTVVSDPPEDEQDLECEDIGVAHVDLADMFQEGRDLIEQNIDVFDARADGEGIGKLRVTVEALHALQSVYKQYRDDLEA 694B83C6A109E50A o75161-1 Nephrocystin-4 Nephroretinin NPHP4 KIAA0673 protein protein human Homo sapiens Human MNDWHRIFTQNVLVPPHPQRARQPWKESTAFQCVLKWLDGPVIRQGVLEVLSEVECHLRVSFFDVTYRHFFGRTWKTTVKPTKRPPSRIVFNEPLYFHTSLNHPHIVAVVEVVAEGKKRDGSLQTLSCGFGILRIFSNQPDSPISASQDKRLRLYHGTPRALLHPLLQDPAEQNRHMTLIENCSLQYTLKPHPALEPAFHLLPENLLVSGLQQIPGLLPAHGESGDALRKPRLQKPITGHLDDLFFTLYPSLEKFEEELLELHVQDHFQEGCGPLDGGALEILERRLRVGVHNGLGFVQRPQVVVLVPEMDVALTRSASFSRKVVSSSKTSSGSQALVLRSRLRLPEMVGHPAFAVIFQLEYVFSSPAGVDGNAASVTSLSNLACMHMVRWAVWNPLLEADSGRVTLPLQGGIQPNPSHCLVYKVPSASMSSEEVKQVESGTLRFQFSLGSEEHLDAPTEPVSGPKVERRPSRKPPTSPSSPPAPVPRVLAAPQNSPVGPGLSISQLAASPRSPTQHCLARPTSQLPHGSQASPAQAQEFPLEAGISHLEADLSQTSLVLETSIAEQLQELPFTPLHAPIVVGTQTRSSAGQPSRASMVLLQSSGFPEILDANKQPAEAVSATEPVTFNPQKEESDCLQSNEMVLQFLAFSRVAQDCRGTSWPKTVYFTFQFYRFPPATTPRLQLVQLDEAGQPSSGALTHILVPVSRDGTFDAGSPGFQLRYMVGPGFLKPGERRCFARYLAVQTLQIDVWDGDSLLLIGSAAVQMKHLLRQGRPAVQASHELEVVATEYEQDNMVVSGDMLGFGRVKPIGVHSVVKGRLHLTLANVGHPCEQKVRGCSTLPPSRSRVISNDGASRFSGGSLLTTGSSRRKHVVQAQKLADVDSELAAMLLTHARQGKGPQDVSRESDATRRRKLERMRSVRLQEAGGDLGRRGTSVLAQQSVRTQHLRDLQVIAAYRERTKAESIASLLSLAITTEHTLHATLGVAEFFEFVLKNPHNTQHTVTVEIDNPELSVIVDSQEWRDFKGAAGLHTPVEEDMFHLRGSLAPQLYLRPHETAHVPFKFQSFSAGQLAMVQASPGLSNEKGMDAVSPWKSSAVPTKHAKVLFRASGGKPIAVLCLTVELQPHVVDQVFRFYHPELSFLKKAIRLPPWHTFPGAPVGMLGEDPPVHVRCSDPNVICETQNVGPGEPRDIFLKVASGPSPEIKDFFVIIYSDRWLATPTQTWQVYLHSLQRVDVSCVAGQLTRLSLVLRGTQTVRKVRAFTSHPQELKTDPKGVFVLPPRGVQDLHVGVRPLRAGSRFVHLNLVDVDCHQLVASWLVCLCCRQPLISKAFEIMLAAGEGKGVNKRITYTNPYPSRRTFHLHSDHPELLRFREDSFQVGGGETYTIGLQFAPSQRVGEEEILIYINDHEDKNEEAFCVKVIYQ 8C974278F4EE4505 nphp4-rpgrip1-1 3 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 16 14 physical association physical association Table 1; 38-40 rpgrip1-1 5 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association no figure rpgr-rpgrip1-5 3 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 23 25 physical association physical association Table 1; 3 rpgr-rpgrip1-6 3 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 28 30 physical association physical association Table 1; 4 rpgrip1-nphp4_bovin-1 3 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association Table 1; 19 and 20 rpgrip1-nphp4-1 3 11 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range 39 37 physical association physical association Table 1; 27 nphp4-rpgrip1-2 3 9 unspecified role unspecified role prey prey Q96KN7-1:p.Arg852Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R Q Wed May 02 17:39:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg852gln' Tue Jun 30 18:51:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg852Gln' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q96KN7-1:p.Gly746Glu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain G E Wed May 02 16:44:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly746glu' Tue Jun 30 18:41:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly746Glu' Q96KN7-1:p.Asp876Gly mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain D G Wed May 02 15:24:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp876gly' Tue Jun 30 16:14:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp876Gly' mutation arg852gln has no significant effect on association 11 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O75161-1:p.Gly754Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain G R Wed May 02 16:58:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly754arg' Tue Jun 30 20:09:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly754Arg' region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain mutations arg740his and arg848trp have no significant effect on association 43 48 physical association physical association Table 1; 28 and Table 2; 8,9, 10, 12 and 13, fig. 5B and D rpgrip1-nphp4-2 3 11 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range 55 53 physical association physical association Table 1; 29-31 nphp4-rpgrip1-3 3 11 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 60 58 physical association physical association Table 1; 48 rpgrip1l-nphp4-4 3 11 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table 1; 47 rpgrip1-nphp4-3 1 9 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 72 69 physical association physical association 2B and supplementary 9B nphp4-rpgrip1-4 1 9 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 76 79 physical association physical association 2C and supplementary 9A rpgrip1-nphp4-4 4 9 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus cyfp cYFP n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 3H nphp4-rpgrip1-6 4 9 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus cyfp cYFP n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization supplemental 10 H,L and P rpgrip1-nphp4-6 2 9 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey cell lysate cell lysate n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 95 98 physical association physical association supplemental 8B nphp4-rpgrip1-7 2 9 unspecified role unspecified role prey prey n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 11 unspecified role unspecified role bait bait cell lysate cell lysate region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 102 104 physical association physical association supplemental 8D rpgrip1-nphp4-7 1 9 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q96KN7-1:p.Asp876Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain D G Wed May 02 18:16:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp876gly' Tue Jun 30 19:08:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp876Gly' region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q96KN7-1:p.Arg852Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R Q Wed May 02 19:42:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg852gln' Tue Jun 30 16:37:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg852Gln' mutation arg852gln has no effect on association 11 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O75161-1:p.Gly754Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain G R Wed May 02 18:13:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly754arg' Tue Jun 30 18:58:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly754Arg' region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain mutant arg740his has no effect on association physical association physical association 5E