IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:Psi-synthase also acting on tRNAs. The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:Psi-synthase also acting on tRNAs. RNA (New York, N.Y.) 2003 imex curation Behm-Ansmant I., Urban A., Ma X., Yu YT., Motorin Y., Branlant C. Yuri.Motorin@maem.uhp-nancy.fr 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro enzymatic study enzymatic study rt-pcr reverse transcription pcr RT-PCR Behm-Ansmant I., Urban A., Ma X., Yu YT., Motorin Y., Branlant C. To reveal the pseudourydilation, the RNA was incubated with CMCT followed by alkaline solution and rt-PCR. Yuri.Motorin@maem.uhp-nancy.fr hopper.64@osu.edu Accepted 2016-MAY-18 AT 13:33 BST AT 13:33 BST by ORCHARD RNA (New York, N.Y.) 2003 imex curation The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:Psi-synthase also acting on tRNAs. The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:Psi-synthase also acting on tRNAs. RNA (New York, N.Y.) 2003 imex curation Behm-Ansmant I., Urban A., Ma X., Yu YT., Motorin Y., Branlant C. Yuri.Motorin@maem.uhp-nancy.fr 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro enzymatic study enzymatic study weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Behm-Ansmant I., Urban A., Ma X., Yu YT., Motorin Y., Branlant C. To detect pseudouridines, the tRNA was digested with T2 Rnase, and TLC fractionated, before autoradiography Yuri.Motorin@maem.uhp-nancy.fr hopper.64@osu.edu Accepted 2016-MAY-18 AT 13:33 BST AT 13:33 BST by ORCHARD RNA (New York, N.Y.) 2003 imex curation trna_tyr _gua tRNATyr GUA trna transfer rna transfer ribonucleic acid tRNA transfer RNA trna yeast Saccharomyces cerevisiae Baker's yeast CUCUCGGUAGCCAAGUUGGUUUAAGGCGCAAGACUGUAAAUCUUGAGAUCGGGCGUUCGACUCGCCCCCGGGAGA pus7_yeast Multisubstrate pseudouridine synthase 7 YOR243C O5254 PUS7 RNA-uridine isomerase 7 RNA pseudouridylate synthase 7 tRNA pseudouridine(13) synthase protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDSSEATVKRPLDAHVGPSENAAKKLKIEQRTQADGIHEADVGITLFLSPELPGFRGQIKQRYTDFLVNEIDQEGKVIHLTDKGFKMPKKPQRSKEEVNAEKESEAARRQEFNVDPELRNQLVEIFGEEDVLKIESVYRTANKMETAKNFEDKSVRTKIHQLLREAFKNELESVTTDTNTFKIARSNRNSRTNKQEKINQTRDANGVENWGYGPSKDFIHFTLHKENKDTMEAVNVITKLLRVPSRVIRYAGTKDRRAVTCQRVSISKIGLDRLNALNRTLKGMIIGNYNFSDASLNLGDLKGNEFVVVIRDVTTGNSEVSLEEIVSNGCKSLSENGFINYFGMQRFGTFSISTHTIGRELLLSNWKKAAELILSDQDNVLPKSKEARKIWAETKDAALALKQMPRQCLAENALLYSLSNQRKEEDGTYSENAYYTAIMKIPRNLRTMYVHAYQSYVWNSIASKRIELHGLKLVVGDLVIDTSEKSPLISGIDDEDFDEDVREAQFIRAKAVTQEDIDSVKYTMEDVVLPSPGFDVLYPSNEELKQLYVDILKADNMDPFNMRRKVRDFSLAGSYRTVIQKPKSLEYRIIHYDDPSQQLVNTDLDILNNTRAKESGQKYMKAKLDRYMPDKGGEKTAVVLKFQLGTSAYATMALRELMKLETSRRGDMCDVKENI 4B63F137B781FCCB trna_asp_guc tRna Asp GUC trna transfer rna transfer ribonucleic acid tRNA transfer RNA trna yeast Saccharomyces cerevisiae Baker's yeast UCCGUGAUAGUUUAAUGGUCAGAAUGGGCGCUUGUCGCGUGCCAGAUCGGGGUUCAAUUCCCCGUCGCGGAG pus7-trna asp guc-1 1 5 enzyme target enzyme target substrate substrate neutral component neutral component Pseudouridilation rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain 4 enzyme enzyme neutral component neutral component tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction Fig5A pus7-trna asp guc-2 2 5 enzyme target enzyme target substrate substrate neutral component neutral component Pseudouridilation rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined u13c mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain U C 4 enzyme enzyme neutral component neutral component tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q08647:p.Asp256Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain D A Wed May 02 19:01:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp256ala' Tue Jun 30 19:27:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp256Ala' ecoli Escherichia coli (strain K12) enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction Fig5B,C,D pus7-trnatyr gua-1 2 3 enzyme target enzyme target substrate substrate neutral component neutral component Pseudourydine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 enzyme enzyme neutral component neutral component tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q08647:p.Asp256Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain D A Wed May 02 18:20:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp256ala' Tue Jun 30 19:01:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp256Ala' ecoli Escherichia coli (strain K12) enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction Fig 6