IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Nicotinic receptor subtypes in the developing chick brain: appearance of a species containing the alpha4, beta2, and alpha5 gene products. Nicotinic receptor subtypes in the developing chick brain: appearance of a species containing the alpha4, beta2, and alpha5 gene products. Mol. Pharmacol. (0026-895X) 1998 imex curation Conroy W.G., Berg D.K. I have not curated any of the Epi-binding expt after discussion with Sandra. Epi is the ligand of many of the AchR subunits and therefore the interactions from cell extracts are not specific by simply detecting relative % Epi-binding after several different rounds of IP. dberg@ucsd.edu Only protein-protein interactions chick-brain Gallus gallus brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Conroy W.G., Berg D.K. dberg@ucsd.edu Accepted 2015-MAR-05 by ORCHARD Mol. Pharmacol. (0026-895X) 1998 Only protein-protein interactions imex curation achb2_chick Neuronal acetylcholine receptor subunit beta-2 CHRNB2 Neuronal acetylcholine receptor non-alpha-1 chain protein protein chick Gallus gallus (Chicken) Chicken MALLRVLCLLAALRRSLCTDTEERLVEYLLDPTRYNKLIRPATNGSQLVTVQLMVSLAQLISVHEREQIMTTNVWLTQEWEDYRLTWKPEDFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVISYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDLVLKSEVASLDDFTPSGEWDIVALPGRRNENPDDSTYVDITYDFIIRRKPLFYTINLIIPCILITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMPPWVRTLFLRKLPALLFMKQPQQNCARQRLRQRRQTQERAAAATLFLRAGARACACYANPGAAKAEGLNGYRERQGQGPDPPAPCGCGLEEAVEGVRFIADHMRSEDDDQSVSEDWKYVAMVIDRLFLWIFVFVCVFGTVGMFLQPLFQNYATNSLLQLGQGTPTSK 984331E8A76D9750 acha4_chick Neuronal acetylcholine receptor subunit alpha-4 CHRNA4 protein protein chick Gallus gallus (Chicken) Chicken MGFLVSKGNLLLLLCASIFPAFGHVETRAHAEERLLKKLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDPQEYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFYDGRIKWMPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDLVSMHSHVDQLDYWESGEWVIINAVGNYNSKKYECCTEIYPDITYSFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSPRTHTMPDWVRRVFLDIVPRLLFMKRPSTVKDNCKKLIESMHKLTNSPRLWSETDMEPNFTTSSSPSPQSNEPSPTSSFCAHLEEPAKPMCKSPSGQYSMLHPEPPQVTCSSPKPSCHPLSDTQTTSISKGRSLSVQQMYSPNKTEEGSIRCRSRSIQYCYLQEDSSQTNGHSSASPASQRCHLNEEQPQHKPHQCKCKCRKGEAAGTPTQGSKSHSNKGEHLVLMSPALKLAVEGVHYIADHLRAEDADFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFLPPWLAGMI 290B035893D6485A acha5_chick Neuronal acetylcholine receptor subunit alpha-5 CHRNA5 protein protein chick Gallus gallus (Chicken) Chicken MPLRARSRKPGAGPAARAPQAGVSEPSFVAKSEDRLFKHLFEDYQRWVRPVEHLNDTIKIKFGLAISQLVDVDEKNQLMTTNVWLKQEWIHVKLRWNPEDYAGITSIRVPSDSIWIPDIVLYDNADGRFEGTSTKTVVKYDGTIAWTPPVNYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDIILEDYEVDKRDFFDNGEWEIVTATGSKGNRTDGCCWYPFVTYSFIIRRLPLFYTLFLIIPCIGLSFLTVLVFYLPSNEAEKISLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIVITVFAINIHHRSSSTHNAMAPWVRKIFLHKLPKLLCMRSHVDRYFAQKEEKGNMSGSESSRNTLEAALDSIRYITRHVMKENEVREVVEDWKFIAQVLDRMFLWAFLLVSIIGSLVLFIPVIHKWASIIVPVHIGSTNT A33D79ED1F799776 chrnb2-chrna4-1 1 4 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association Fig 6b (lanes 1, 3, 4, 5, 7, 8) IPs were carried out with or without initial immunodepletion of alpha4-containing receptors. In the case of alpha4-deletion some alpha4 remained in the beta2 IP (lane 4) but binding of alpha5 to beta2 was much stronger (lane 8). chrnb2-chrna5-3 1 2 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association Fig 6b (lanes 2 & 6) Initial immunodepletion of alpha4-containing receptors resulted in weak binding of alpha5 to beta2 (lane 6).