UniProt UniProt http://www.uniprot.org Vps33b pathogenic mutations preferentially affect VIPAS39/SPE-39-positive endosomes. Vps33b pathogenic mutations preferentially affect VIPAS39/SPE-39-positive endosomes. Human molecular genetics 2013 imex curation Tornieri K., Zlatic SA., Mullin AP., Werner E., Harrison R., L'hernault SW., Faundez V. vfaunde@emory.edu 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Tornieri K., Zlatic SA., Mullin AP., Werner E., Harrison R., L'hernault SW., Faundez V. vfaunde@emory.edu Accepted 2015-NOV-24 AT 16:58 GMT AT 16:58 GMT by PPORRAS Human molecular genetics 2013 IMEx imex curation Vps33b pathogenic mutations preferentially affect VIPAS39/SPE-39-positive endosomes. Vps33b pathogenic mutations preferentially affect VIPAS39/SPE-39-positive endosomes. Human molecular genetics 2013 imex curation Tornieri K., Zlatic SA., Mullin AP., Werner E., Harrison R., L'hernault SW., Faundez V. vfaunde@emory.edu 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Tornieri K., Zlatic SA., Mullin AP., Werner E., Harrison R., L'hernault SW., Faundez V. vfaunde@emory.edu Accepted 2015-NOV-24 AT 16:58 GMT AT 16:58 GMT by PPORRAS Human molecular genetics 2013 imex curation Vps33b pathogenic mutations preferentially affect VIPAS39/SPE-39-positive endosomes. Vps33b pathogenic mutations preferentially affect VIPAS39/SPE-39-positive endosomes. Human molecular genetics 2013 imex curation Tornieri K., Zlatic SA., Mullin AP., Werner E., Harrison R., L'hernault SW., Faundez V. vfaunde@emory.edu 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Tornieri K., Zlatic SA., Mullin AP., Werner E., Harrison R., L'hernault SW., Faundez V. vfaunde@emory.edu Accepted 2015-NOV-24 AT 16:58 GMT AT 16:58 GMT by PPORRAS Human molecular genetics 2013 IMEx imex curation Vps33b pathogenic mutations preferentially affect VIPAS39/SPE-39-positive endosomes. Vps33b pathogenic mutations preferentially affect VIPAS39/SPE-39-positive endosomes. Human molecular genetics 2013 imex curation Tornieri K., Zlatic SA., Mullin AP., Werner E., Harrison R., L'hernault SW., Faundez V. vfaunde@emory.edu 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Tornieri K., Zlatic SA., Mullin AP., Werner E., Harrison R., L'hernault SW., Faundez V. vfaunde@emory.edu Accepted 2015-NOV-24 AT 16:58 GMT AT 16:58 GMT by PPORRAS Human molecular genetics 2013 imex curation vps39_human Vam6/Vps39-like protein KIAA0770 VPS39 VAM6 TLP TRAP1-like protein protein protein human Homo sapiens Human MHDAFEPVPILEKLPLQIDCLAAWEEWLLVGTKQGHLLLYRIRKDVVPADVASPESGSCNRFEVTLEKSNKNFSKKIQQIHVVSQFKILVSLLENNIYVHDLLTFQQITTVSKAKGASLFTCDLQHTETGEEVLRMCVAVKKKLQLYFWKDREFHELQGDFSVPDVPKSMAWCENSICVGFKRDYYLIRVDGKGSIKELFPTGKQLEPLVAPLADGKVAVGQDDLTVVLNEEGICTQKCALNWTDIPVAMEHQPPYIIAVLPRYVEIRTFEPRLLVQSIELQRPRFITSGGSNIIYVASNHFVWRLIPVPMATQIQQLLQDKQFELALQLAEMKDDSDSEKQQQIHHIKNLYAFNLFCQKRFDESMQVFAKLGTDPTHVMGLYPDLLPTDYRKQLQYPNPLPVLSGAELEKAHLALIDYLTQKRSQLVKKLNDSDHQSSTSPLMEGTPTIKSKKKLLQIIDTTLLKCYLHTNVALVAPLLRLENNHCHIEESEHVLKKAHKYSELIILYEKKGLHEKALQVLVDQSKKANSPLKGHERTVQYLQHLGTENLHLIFSYSVWVLRDFPEDGLKIFTEDLPEVESLPRDRVLGFLIENFKGLAIPYLEHIIHVWEETGSRFHNCLIQLYCEKVQGLMKEYLLSFPAGKTPVPAGEEEGELGEYRQKLLMFLEISSYYDPGRLICDFPFDGLLEERALLLGRMGKHEQALFIYVHILKDTRMAEEYCHKHYDRNKDGNKDVYLSLLRMYLSPPSIHCLGPIKLELLEPKANLQAALQVLELHHSKLDTTKALNLLPANTQINDIRIFLEKVLEENAQKKRFNQVLKNLLHAEFLRVQEERILHQQVKCIITEEKVCMVCKKKIGNSAFARYPNGVVVHYFCSKEVNPADT E5A40A3C787EB112 vps41_human Vacuolar protein sorting-associated protein 41 homolog VPS41 S53 protein protein human Homo sapiens Human MAEAEEQETGSLEESTDESEEEESEEEPKLKYERLSNGVTEILQKDAASCMTVHDKFLALGTHYGKVYLLDVQGNITQKFDVSPVKINQISLDESGEHMGVCSEDGKVQVFGLYSGEEFHETFDCPIKIIAVHPHFVRSSCKQFVTGGKKLLLFERSWMNRWKSAVLHEGEGNIRSVKWRGHLIAWANNMGVKIFDIISKQRITNVPRDDISLRPDMYPCSLCWKDNVTLIIGWGTSVKVCSVKERHASEMRDLPSRYVEIVSQFETEFYISGLAPLCDQLVVLSYVKEISEKTEREYCARPRLDIIQPLSETCEEISSDALTVRGFQENECRDYHLEYSEGESLFYIVSPRDVVVAKERDQDDHIDWLLEKKKYEEALMAAEISQKNIKRHKILDIGLAYINHLVERGDYDIAARKCQKILGKNAALWEYEVYKFKEIGQLKAISPYLPRGDPVLKPLIYEMILHEFLESDYEGFATLIREWPGDLYNNSVIVQAVRDHLKKDSQNKTLLKTLAELYTYDKNYGNALEIYLTLRHKDVFQLIHKHNLFSSIKDKIVLLMDFDSEKAVDMLLDNEDKISIKKVVEELEDRPELQHVYLHKLFKRDHHKGQRYHEKQISLYAEYDRPNLLPFLRDSTHCPLEKALEICQQRNFVEETVYLLSRMGNSRSALKMIMEELHDVDKAIEFAKEQDDGELWEDLILYSIDKPPFITGLLNNIGTHVDPILLIHRIKEGMEIPNLRDSLVKILQDYNLQILLREGCKKILVADSLSLLKKMHRTQMKGVLVDEENICESCLSPILPSDAAKPFSVVVFHCRHMFHKECLPMPSMNSAAQFCNICSAKNRGPGSAILEMKK 037577D5FA3A18A0 vp33b_human Vacuolar protein sorting-associated protein 33B VPS33B protein protein human Homo sapiens Human MAFPHRPDAPELPDFSMLKRLARDQLIYLLEQLPGKKDLFIEADLMSPLDRIANVSILKQHEVDKLYKVENKPALSSNEQLCFLVRPRIKNMRYIASLVNADKLAGRTRKYKVIFSPQKFYACEMVLEEEGIYGDVSCDEWAFSLLPLDVDLLSMELPEFFRDYFLEGDQRWINTVAQALHLLSTLYGPFPNCYGIGRCAKMAYELWRNLEEEEDGETKGRRPEIGHIFLLDRDVDFVTALCSQVVYEGLVDDTFRIKCGSVDFGPEVTSSDKSLKVLLNAEDKVFNEIRNEHFSNVFGFLSQKARNLQAQYDRRRGMDIKQMKNFVSQELKGLKQEHRLLSLHIGACESIMKKKTKQDFQELIKTEHALLEGFNIRESTSYIEEHIDRQVSPIESLRLMCLLSITENGLIPKDYRSLKTQYLQSYGPEHLLTFSNLRRAGLLTEQAPGDTLTAVESKVSKLVTDKAAGKITDAFSSLAKRSNFRAISKKLNLIPRVDGEYDLKVPRDMAYVFGGAYVPLSCRIIEQVLERRSWQGLDEVVRLLNCSDFAFTDMTKEDKASSESLRLILVVFLGGCTFSEISALRFLGREKGYRFIFLTTAVTNSARLMEAMSEVKA BD7DB97E1FEB1D32 vps18_mouse Vacuolar protein sorting-associated protein 18 homolog Vps18 protein protein mouse Mus musculus Mouse MASILDEYEDSLSRSAVLQTGCPSVGIPHSGYVSAHLEKEVPIFTKQRVDFTPSERITSLVVSCNQLCMSLGKDTLLRIDLGKASEPNRVELGRKDDAKVHKMFLDHTGSHLLVALSSTEVLYMNRNGQKARPLARWKGQLVESVGWNKAMGNESSTGPILVGTAQGQIFEAELSASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDGRGFVIATTRQRLFQFIGRAVEDTEAQGFAGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGSLDCGRPDSLLSEERVWEYPAGVGPGANPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVLRDHFLEKFGPLRHMVKDSSTGHLWAYTERAVFRYHVQREARDVWRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQHRYLESARCYALTQSYFEEIALKFLEARQEEALAEFLQRKLAGLKPTERTQATLLTTWLTELYLSRLGALQGDPDALTLYRDTRECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGEAQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGQPASLLAYLEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASCPLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCSTCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPTKGSVKAKEAEAGAAAVGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQRYEEEHLSWL 6B4E90FC39DC897F vps11_mouse Vacuolar protein sorting-associated protein 11 homolog Vps11 protein protein mouse Mus musculus Mouse MAAYLQWRRFVFFEKELVKEPLGNDGAAPGAAPVSGSAASKFLCLPPGITVCDSGRGSLVFGDMEGQIWFLPRSLQLTGFQAYKLRVTHLYQLKQHNILASVGEDEEGINPLVKIWNLEKRDGGNPLCTRIFPAIPGTEPTVVSCLTVHENLNFMAIGFTDGSVTLNKGDITRDRHSKTQILHKGNYPVTGLAFRQAGKTTHLFVVTTENVQSYIVSGKDYPRVELDTHGCGLRCSALSDPSQDLQFIVAGDECVYLYQPDERGPCFAFEGHKLIVHWFRGYLVIVSRDRKVSPKSEFTSRDSQNSDKQILNIYDLCNKFIAYSAGFEDIVDVLAEWGSLYVLTRDGRVHALQEKDTQTKLEMLFKKNLFEMAINLAKSQHLDSDGLAQIFMQYGDHLYSKGNHDGAVQQYIRTIGKLEPSYVIRKFLDAQRIHNLTAYLQTLHRQSLANADHTTLLLNCYTKLKDSSKLEEFIKTKSESEVHFDVETAIKVLRQAGYYSHALYLAENHAHHEWYLKIQLEDIKNYQEALRYIGKLPFEQAESNMKRYGKTLMHHIPEQTTQLLKGLCTDYRPSLEGRGDREALSCRASSEEFIPIFANNPRELKAFLEHMSEVQPDSPQGIYDTLLELRLQNWAHEKDPQAKEKLHAEAISLLKSGRFCDVFDKALVLCQMHDFQDGVLYLYEQGKLFQQIMHYHMQHEQYRQVIAVCERHGEQEPSLWEQALSYFARKEEDCKEYVAAVLRHIENKSLMPPLLVVQTLAHNSTATLSIIRDYLVQKLQKQSQQIAQDELRVRRYREETTRIRQEIQELKASPKIFQKTKCSICNSALELPSVHFLCGHSFHQHCFESYSESDADCPTCLPENRKVMDMIRAQEQKRDLHDQFQHQLKCSNDSFSVIADYFGRGVFNKLTLLTDPPTARLTPSLEAGLQRDLLMHSRRGT DB96F07E92C9E534 stx7_human Syntaxin-7 STX7 protein protein human Homo sapiens Human MSYTPGVGGDPAQLAQRISSNIQKITQCSVEIQRTLNQLGTPQDSPELRQQLQQKQQYTNQLAKETDKYIKEFGSLPTTPSEQRQRKIQKDRLVAEFTTSLTNFQKVQRQAAEREKEFVARVRASSRVSGSFPEDSSKERNLVSWESQTQPQVQVQDEEITEDDLRLIHERESSIRQLEADIMDINEIFKDLGMMIHEQGDVIDSIEANVENAEVHVQQANQQLSRAADYQRKSRKTLCIIILILVIGVAIISLIIWGLNH DIP protein O15400 original sequence version: 123 DIP protein O15400 original sequence version: 116 42AC173F0233ACDA spe39_human Spermatogenesis-defective protein 39 homolog VIPAS39 C14orf133 SPE39 VIPAR VPS33B-interacting protein in apical-basolateral polarity regulator VPS33B-interacting protein in polarity and apical restriction protein protein human Homo sapiens Human MNRTKGDEEEYWNSSKFKAFTFDDEDDELSQLKESKRAVNSLRDFVDDDDDDDLERVSWSGEPVGSISWSIRETAGNSGSTHEGREQLKSRNSFSSYAQLPKPTSTYSLSSFFRGRTRPGSFQSLSDALSDTPAKSYAPELGRPKGEYRDYSNDWSPSDTVRRLRKGKVCSLERFRSLQDKLQLLEEAVSMHDGNVITAVLIFLKRTLSKEILFRELEVRQVALRHLIHFLKEIGDQKLLLDLFRFLDRTEELALSHYREHLNIQDPDKRKEFLKTCVGLPFSAEDSAHIQDHYTLLERQIIIEANDRHLESAGQTEIFRKHPRKASILNMPLVTTLFYSCFYHYTEAEGTFSSPVNLKKTFKIPDKQYVLTALAARAKLRAWNDVDALFTTKNWLGYTKKRAPIGFHRVVEILHKNNAPVQILQEYVNLVEDVDTKLNLATKFKCHDVVIDTYRDLKDRQQLLAYRSKVDKGSAEEEKIDALLSSSQIRWKN CF05C38EB922D192 vipas39-vps33b-5 1 7 unspecified role unspecified role prey prey Q9H267:p.Leu30Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L P Disease variant in ARC1; UniProt FTId: VAR_018983 Wed May 02 19:27:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu30pro' Tue Jun 30 16:20:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu30Pro' region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q9H267:p.Gly249Val mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G V Corresponds to variant in Drosophila carnation phenotype Wed May 02 14:36:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly249val' Tue Jun 30 15:49:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly249Val' Following variants/mutations interact: ser243phe (ARC1; UniProt FTId: VAR_057901); asp234his; asp252glu (corresponds to mouse buff phenotype). 11 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association 1B and 2D, 7A-C vps33b-vipas39-3 1 7 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 1C vps11-vps39-2 1 5 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association 1B and C vps33b-vipas39-4 4 11 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection Q9H267:p.Gly249Val mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G V Wed May 02 20:01:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly249val' Tue Jun 30 15:51:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly249Val' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9H267:p.Leu30Pro mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L P Wed May 02 16:11:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu30pro' Tue Jun 30 17:36:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu30Pro' Following mutations interact without significant changes: ser243phe and asp234his physical association physical association 3A-D The interaction was tested with and without DSP as crosslinking agent, which was supposed to stabilize interactions potentially weakened by Vps33b pathogenic mutations. However the crosslinking agent had no visible effect in the otucome of the interaction. vps33b-vps41-1 2 6 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Following mutations interact: leu30pro and asp234his physical association physical association 4A vps33b-vps18-1 2 8 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Following mutations interact: leu30pro and asp234his physical association physical association 4B vipas39-vps33b-6 1 7 unspecified role unspecified role unspecified role unspecified role Q9H267:p.Val235_Phe237delinsAlaAlaAla mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain VDF AAA Wed May 02 19:43:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_asp_phe235-237ala_ala_ala' Tue Jun 30 20:05:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val235_Phe237delinsAlaAlaAla' Q9H267:p.Asp232_Asp234delinsAlaAlaAla mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain DRD AAA Wed May 02 16:37:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_arg_asp232-234ala_ala_ala' Tue Jun 30 17:58:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp232_Asp234delinsAlaAlaAla' Q9H267:p.Val251_Asp253delinsAlaAlaAla mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain VDD AAA Wed May 02 16:06:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_asp_asp251-253ala_ala_ala' Tue Jun 30 17:35:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val251_Asp253delinsAlaAlaAla' Following variants/mutations interact: ser243phe (ARC1; UniProt FTId: VAR_057901); asp234his; asp252glu (corresponds to mouse buff phenotype). 11 unspecified role unspecified role unspecified role unspecified role physical association physical association 6B Bait/prey relationship cannot be established stx7-vps33b-1 1 7 unspecified role unspecified role prey prey Following mutations interact: asp234his, gly249val, leu30pro, ser243phe, asp252glu 10 unspecified role unspecified role bait bait target snare coiled-coil domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 7A-C stx7-vps33b-2 3 10 unspecified role unspecified role neutral component neutral component 7 anti tag immunost anti tag immunostaining anti tag immunost immunostaining immunostaining unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Following mutations show colocalization: leu30pro, asp234his, gly249val colocalization colocalization 8A vps33b-1 3 7 anti tag immunost anti tag immunostaining anti tag immunost immunostaining immunostaining unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection Q9H267:p.Leu30Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L P Wed May 02 18:13:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu30pro' Tue Jun 30 18:40:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu30Pro' Q9H267:p.Asp234His mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain D H Wed May 02 15:23:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp234his' Tue Jun 30 19:34:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp234His' Q9H267:p.Ser243Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain S F Wed May 02 17:49:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser243phe' Tue Jun 30 17:29:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser243Phe' Q9H267:p.Gly249Val mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G V Wed May 02 15:32:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly249val' Tue Jun 30 16:58:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly249Val' Q9H267:p.Asp252Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain D E Wed May 02 20:24:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp252glu' Tue Jun 30 19:48:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp252Glu' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role neutral component neutral component colocalization colocalization 9A, 10C