UniProt UniProt http://www.uniprot.org Crystal structures of the catalytic domain of human soluble guanylate cyclase. Crystal structures of the catalytic domain of human soluble guanylate cyclase. PloS one 2012 imex curation Allerston CK., von Delft F., Gileadi O. opher.gileadi@sgc.ox.ac.uk 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Allerston CK., von Delft F., Gileadi O. opher.gileadi@sgc.ox.ac.uk Accepted 2015-AUG-05 by MGT PloS one 2012 IMEx MIMIx Crystal structures of the catalytic domain of human soluble guanylate cyclase. Crystal structures of the catalytic domain of human soluble guanylate cyclase. PloS one 2012 imex curation Allerston CK., von Delft F., Gileadi O. opher.gileadi@sgc.ox.ac.uk 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro gtpase assay gtpase assay GTPase gtp hydrolisis gtp hydrolisis GTPase predetermined predetermined participant predetermined Allerston CK., von Delft F., Gileadi O. Results from assay of Full-length soluble guanylate cyclase (bovine lung) have not been included as the isozyme/subunit composition of the enzyme is not known. opher.gileadi@sgc.ox.ac.uk Accepted 2015-AUG-06 by MGT PloS one 2012 Only protein-protein interactions imex curation IMEx mimix curation gtp guanosine triphosphate - GTP 5'-GTP GTP Guanosine 5'-triphosphate guanosine 5'-triphosphoric acid guanosine triphosphate GUANOSINE-5'-TRIPHOSPHATE H4gtp guanosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 gcya1_human Guanylate cyclase soluble subunit alpha-1 GUCY1A1 GUC1A3 GUCSA3 GUCY1A3 Guanylate cyclase soluble subunit alpha-3 Soluble guanylate cyclase large subunit protein protein human Homo sapiens Human MFCTKLKDLKITGECPFSLLAPGQVPNESSEEAAGSSESCKATVPICQDIPEKNIQESLPQRKTSRSRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKIKESRKSLEREDFEKTIAEQAVAAGVPVEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQSSHCQEAGKRGRLEDASILCLDKEDDFLHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLMPPCFHNDCSEFVNQPYLLYSVHMKSTKPSLSPSKPQSSLVIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDFQGKPNFEEYFEILTPKINQTFSGIMTMLNMQFVVRVRRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALEEEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDTHAVQIALMALKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPPNFPSEIPGICHFLDAYQQGTNSKPCFQKKDVEDGNANFLGKASGID DA1E14A5E11451CF gcyb1_human Guanylate cyclase soluble subunit beta-1 GUCY1B1 GUC1B3 GUCSB3 GUCY1B3 Guanylate cyclase soluble subunit beta-3 Soluble guanylate cyclase small subunit protein protein human Homo sapiens Human MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPYTFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVLRSKEGLLDVEKLECEDELTGTEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAGQVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSEYTYRCLMSPENSDPQFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETKQDDD 231E4E660DE02AA1 gucy1a3-gucy1b3-1 1 5 unspecified role unspecified role neutral component neutral component catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q02153:p.[Gly476Cys;Cys541Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain G C certain certain sequence position certain certain certain sequence position certain C S Wed May 02 14:53:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly476cys,cys541ser' Tue Jun 30 17:26:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gly476Cys;Cys541Ser]' ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 2.08 Å R-working: 0.167 R-free: 0.209 gucy1b3-1 1 5 unspecified role unspecified role neutral component neutral component catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 1.63 Å R-working: 0.184 R-free: 0.219 Table 1 gucy1a3-gucy1b3-3 2 5 enzyme enzyme neutral component neutral component catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q02153:p.[Gly476Cys;Cys541Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain G C certain certain sequence position certain certain certain sequence position certain C S Wed May 02 16:13:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly476cys,cys541ser' Tue Jun 30 19:31:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gly476Cys;Cys541Ser]' ecoli Escherichia coli (strain K12) 4 enzyme enzyme neutral component neutral component catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 3 enzyme target enzyme target substrate substrate neutral component neutral component region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 20 17 gtpase reaction gtpase reaction Table 2 The enzyme active-site is contributed to by residues from both subunits, and lies at the interface between the two catalytic domains. Specific Activity is 25-300 nmol/mg/min. (full length bovine enzyme has a specific activity of 4300 nmol/mg/min.)