HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Interaction domain of glycoproteins gB and gH of Marek's disease virus and identification of an antiviral peptide with dual functions. Interaction domain of glycoproteins gB and gH of Marek's disease virus and identification of an antiviral peptide with dual functions. PloS one 2012 imex curation Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M., Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M. wangxj@cau.edu.cn 19-Jan-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M., Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M. wangxj@cau.edu.cn Accepted 2015-DEC-15 AT 13:06 GMT AT 13:06 GMT by PPORRAS PloS one 2012 Only protein-protein interactions imex curation imex curation Interaction domain of glycoproteins gB and gH of Marek's disease virus and identification of an antiviral peptide with dual functions. Interaction domain of glycoproteins gB and gH of Marek's disease virus and identification of an antiviral peptide with dual functions. PloS one 2012 imex curation Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M., Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M. wangxj@cau.edu.cn 19-Jan-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro cd circular dichroism CD CD cd predetermined predetermined participant predetermined Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M., Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M. wangxj@cau.edu.cn Accepted 2015-DEC-15 AT 13:06 GMT AT 13:06 GMT by PPORRAS PloS one 2012 Only protein-protein interactions imex curation imex curation Interaction domain of glycoproteins gB and gH of Marek's disease virus and identification of an antiviral peptide with dual functions. Interaction domain of glycoproteins gB and gH of Marek's disease virus and identification of an antiviral peptide with dual functions. PloS one 2012 imex curation Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M., Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M. wangxj@cau.edu.cn 19-Jan-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation chick-embryonic_fibroblast Chick embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M., Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M. wangxj@cau.edu.cn Accepted 2015-DEC-15 AT 13:06 GMT AT 13:06 GMT by PPORRAS PloS one 2012 Only protein-protein interactions imex curation imex curation Interaction domain of glycoproteins gB and gH of Marek's disease virus and identification of an antiviral peptide with dual functions. Interaction domain of glycoproteins gB and gH of Marek's disease virus and identification of an antiviral peptide with dual functions. PloS one 2012 imex curation Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M., Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M. wangxj@cau.edu.cn 19-Jan-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M., Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M. wangxj@cau.edu.cn Accepted 2015-DEC-15 AT 13:06 GMT AT 13:06 GMT by PPORRAS PloS one 2012 Only protein-protein interactions imex curation imex curation gh_gahvr Envelope glycoprotein H protein protein Gallid herpesvirus 2 Gallid herpesvirus 2 SPPGHGAHVRENVELOPEGLYCOPROTEINHOSGALLIDHERPESVIRUSSTRAINRBBGNGHPESVMGLPGSIVFLIMIHAFCAKKTPTNTLPSLLSLLGITDLPSLRLNILSLDGSANNQGSWVRDNTTFVYIGASSPANGVLFYMPTSHVQQMTFYKRPVSKLLASNNLIKFLNTGSYINHSFMTAMPPYRRNVQIPSDRSGLKLDDKDDAQPTGTNPPTELKNLKPIDVVNPEHRFILTSELTGTYVKHVCFVDPMDMLIPVDYAHIRTIIFGSDGAEVIMKIGITFASITISMKSAPPVELILSERARNISLIWPALKPYEPVDKFTRRPYLIYLLGPHMNASDMEIKSYINMIESVEESSNYDFQIAQTHAQLFIFAATPISDINDIYCFRVVTTRLFMSLVASVRNAFQSGYISFDEIIKTEANIKMITETLSTFALHSNPGTYFLLSGMHLRNENADIIKSLIRKTIINASKNTASLSILQHLYVLRSAYAFNISQESGNLGEHVSSISLELIIALHEESVRDTIAWNTSARHALYYAFASIFQRPPNEWDASRTARKALLFASSMCTEEHIVATELVIQEMYIKINVKNSPVHILDVYTPCVTALRMDISEHHHRLYAMSDVILHPVIEKYLENDSRGIDAEEELETKAELVITKLKTPLMRRLTIYASEVVTCSDADILEATALLVLPISGLGSYVVTRQLGIRGIVYNVDGVDVNNQLYITYVRLPCTTTAGNIVPMVLPRPLGSDCPYCGCVLLRYSTNGNLRHTIYISSQDLQRELIAGGNSSIRYFNPTIAQIYGTSLLLYPNGTIVRILAFESERVTIISATYVATATAGASIAISIAIITVRMRINNFRYNYHRYKKLSLYDDL gb_gahvr Envelope glycoprotein B protein protein Gallid herpesvirus 2 Gallid herpesvirus 2 SPPGBGAHVRENVELOPEGLYCOPROTEINBOSGALLIDHERPESVIRUSSTRAINRBBGNGBPESVMHYFRRNCIFFLIVILYGTNSSPSTQNVTSREVVSSVQLSEEESTFYLCPPPVGSTVIRLEPPRKCPEPRKATEWGEGIAILFKENISPYKFKVTLYYKNIIQTTTWTGTTYRQITNRYTDRTPVSIEEITDLIDGKGRCSSKARYLRNNVYVEAFDRDAGEKQVLLKPSKFNTPESRAWHTTNETYTVWGSPWIYRTGTSVNCIVEEMDARSVFPYSYFAMANGDIANISPFYGLSPPEAAAEPMGYPQDNFKQLDSYFSMDLDKRRKASLPVKRNFLITSHFTVGWDWAPKTTRVCSMTKWKEVTEMLRATVNGRYRFMARELSATFISNTTEFDPNRIILGQCIKREAEAAIEQIFRTKYNDSHVKVGHVQYFLALGGFIVAYQPVLSKSLAHMYLRELMRDNRTDEMLDLVNNKHAIYKKNATSLSRLRRDIRNAPNRKITLDDTTAIKSTSSVQFAMLQFLYDHIQTHINDMFSRIATAWCELQNRELVLWHEGIKINPSATASATLGRRVAAKMLGDVAAVSSCTAIDAESVTLQNSMRVITSTNTCYSRPLVLFSYGENQGNIQGQLGENNELLPTLEAVEPCSANHRRYFLFGSGYALFENYNFVKMVDAADIQIASTFVELNLTLLEDREILPLSVYTKEELRDVGVLDYAEVARRNQLHELKFYDINKVIEVDTNYAFMNGLAELFNGMGQVGQAIGKVVVGAAGAIVSTISGVSAFMSNPFGALAIGLIIIAGLVAAFLAYRYVNKLKSNPMKALYPMTTEVLKAQATRELHGEESDDLERTSIDERKLEEAREMIKYMALVSAEERHEKKLRRKRRGTTAVLSDHLAKMRIKNSNPKYDKLPTTYSDSEDDAV gb-gh-1 3 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 2 gb-gh-2 4 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 3 gb_gahvr-gh_gahvr-1 3 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 2 gh-gh-1 4 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3 gb-gh-5 4 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 3 gb-gh-3 1 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range direct interaction direct interaction 4 and table 1 gh-gh-2 1 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction 4 and table 1 gb-gh-4 1 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range direct interaction direct interaction 4 and table 1 gh_gahvr-1 1 5 unspecified role unspecified role putative self putative self region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain self interaction self interaction 4 and table 1 gb_gahvr-1 1 6 unspecified role unspecified role putative self putative self region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range self interaction self interaction 4 and table 1 gb_gahvr-gh_gahvr-3 1 5 competitor competitor competitor unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range association association 4 and table 1 gh_gahvr-gh_gahvr-1 2 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction 5 and 6 gb_gahvr-gh_gahvr-4 2 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range direct interaction direct interaction 5 and 6 gb_gahvr-gh_gahvr-5 2 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range direct interaction direct interaction 5 and 6 gb_gahvr-gh_gahvr-2 2 5 competitor competitor competitor unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range physical association physical association 5 gh_gahvr-2 2 5 unspecified role unspecified role putative self putative self region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain self interaction self interaction 5 gb_gahvr-6 2 6 unspecified role unspecified role putative self putative self region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range self interaction self interaction 5 gh_gahvr-3 2 5 unspecified role unspecified role putative self putative self region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain self interaction self interaction 5