UniProt UniProt http://www.uniprot.org PHLPP-mediated dephosphorylation of S6K1 inhibits protein translation and cell growth. PHLPP-mediated dephosphorylation of S6K1 inhibits protein translation and cell growth. Molecular and cellular biology 2011 imex curation Liu J., Stevens PD., Li X., Schmidt MD., Gao T., Liu J., Stevens PD., Li X., Schmidt MD., Gao T. Origin of PHLPP1/2 used for in vitro dephosphorylation assay according refs 3 and 8 (PubMed 15808505 and 17386267, resp.). Origin of HA-tagged RPS6KB1 (provided by J.Blenis, Harvard Medical School) according Addgene. (De)phosphorylation sites in RPS6KB1 have been mapped to the UniProt canonical form as it is present in different isoforms and the identity of the transfected isoform cannot be established. tianyan.gao@uky.edu Only protein-protein interactions human-ht_29 Homo sapiens HT-29 caucasian women with colon adenocarcinoma ht_29 HT-29 confirmed as human with IEF of AST, LDH established from the primary tumor of a 44-year-old Caucasian woman with colon adenocarcinoma in 1964; described to be heterotransplantable forming well-differentiated grade I tumors anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Liu J., Stevens PD., Li X., Schmidt MD., Gao T. tianyan.gao@uky.edu tianyan@uky.edu Accepted 2015-JUN-25 by ORCHARD Molecular and cellular biology 2011 IMEx mimix curation PHLPP-mediated dephosphorylation of S6K1 inhibits protein translation and cell growth. PHLPP-mediated dephosphorylation of S6K1 inhibits protein translation and cell growth. Molecular and cellular biology 2011 imex curation Liu J., Stevens PD., Li X., Schmidt MD., Gao T., Liu J., Stevens PD., Li X., Schmidt MD., Gao T. Origin of PHLPP1/2 used for in vitro dephosphorylation assay according refs 3 and 8 (PubMed 15808505 and 17386267, resp.). Origin of HA-tagged RPS6KB1 (provided by J.Blenis, Harvard Medical School) according Addgene. (De)phosphorylation sites in RPS6KB1 have been mapped to the UniProt canonical form as it is present in different isoforms and the identity of the transfected isoform cannot be established. tianyan.gao@uky.edu Only protein-protein interactions in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined Liu J., Stevens PD., Li X., Schmidt MD., Gao T., Liu J., Stevens PD., Li X., Schmidt MD., Gao T. Origin of PHLPP1/2 used for in vitro dephosphorylation assay according refs 3 and 8 (PubMed 15808505 and 17386267, resp.). Origin of HA-tagged RPS6KB1 (provided by J.Blenis, Harvard Medical School) according Addgene. tianyan.gao@uky.edu tianyan@uky.edu Accepted 2015-JUN-25 by ORCHARD Molecular and cellular biology 2011 IMEx mimix curation PHLPP-mediated dephosphorylation of S6K1 inhibits protein translation and cell growth. PHLPP-mediated dephosphorylation of S6K1 inhibits protein translation and cell growth. Molecular and cellular biology 2011 imex curation Liu J., Stevens PD., Li X., Schmidt MD., Gao T., Liu J., Stevens PD., Li X., Schmidt MD., Gao T. Origin of PHLPP1/2 used for in vitro dephosphorylation assay according refs 3 and 8 (PubMed 15808505 and 17386267, resp.). Origin of HA-tagged RPS6KB1 (provided by J.Blenis, Harvard Medical School) according Addgene. (De)phosphorylation sites in RPS6KB1 have been mapped to the UniProt canonical form as it is present in different isoforms and the identity of the transfected isoform cannot be established. tianyan.gao@uky.edu Only protein-protein interactions human-293e Human HEK293E or 293 c18 cell line 293e HEK293E or 293 c18 http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-10852&Template=cellBiology This line is a derivative of 293 (see ATCCCRL-1573) the cells express EBNA1. (Epstein Barr nuclear antigen) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Liu J., Stevens PD., Li X., Schmidt MD., Gao T. tianyan.gao@uky.edu tianyan@uky.edu Accepted 2015-JUN-25 by ORCHARD Molecular and cellular biology 2011 IMEx mimix curation PHLPP-mediated dephosphorylation of S6K1 inhibits protein translation and cell growth. PHLPP-mediated dephosphorylation of S6K1 inhibits protein translation and cell growth. Molecular and cellular biology 2011 imex curation Liu J., Stevens PD., Li X., Schmidt MD., Gao T., Liu J., Stevens PD., Li X., Schmidt MD., Gao T. Origin of PHLPP1/2 used for in vitro dephosphorylation assay according refs 3 and 8 (PubMed 15808505 and 17386267, resp.). Origin of HA-tagged RPS6KB1 (provided by J.Blenis, Harvard Medical School) according Addgene. (De)phosphorylation sites in RPS6KB1 have been mapped to the UniProt canonical form as it is present in different isoforms and the identity of the transfected isoform cannot be established. tianyan.gao@uky.edu Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Liu J., Stevens PD., Li X., Schmidt MD., Gao T., Liu J., Stevens PD., Li X., Schmidt MD., Gao T. Origin of PHLPP1/2 used for in vitro dephosphorylation assay according refs 3 and 8 (PubMed 15808505 and 17386267, resp.). Origin of HA-tagged RPS6KB1 (provided by J.Blenis, Harvard Medical School) according Addgene. (De)phosphorylation sites in RPS6KB1 have been mapped to the UniProt canonical form as it is present in different isoforms and the identity of the transfected isoform cannot be established. tianyan.gao@uky.edu tianyan@uky.edu Accepted 2015-JUN-25 by ORCHARD Molecular and cellular biology 2011 imex curation PHLPP-mediated dephosphorylation of S6K1 inhibits protein translation and cell growth. PHLPP-mediated dephosphorylation of S6K1 inhibits protein translation and cell growth. Molecular and cellular biology 2011 imex curation Liu J., Stevens PD., Li X., Schmidt MD., Gao T., Liu J., Stevens PD., Li X., Schmidt MD., Gao T. Origin of PHLPP1/2 used for in vitro dephosphorylation assay according refs 3 and 8 (PubMed 15808505 and 17386267, resp.). Origin of HA-tagged RPS6KB1 (provided by J.Blenis, Harvard Medical School) according Addgene. (De)phosphorylation sites in RPS6KB1 have been mapped to the UniProt canonical form as it is present in different isoforms and the identity of the transfected isoform cannot be established. tianyan.gao@uky.edu Only protein-protein interactions mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Liu J., Stevens PD., Li X., Schmidt MD., Gao T., Liu J., Stevens PD., Li X., Schmidt MD., Gao T. Origin of PHLPP1/2 used for in vitro dephosphorylation assay according refs 3 and 8 (PubMed 15808505 and 17386267, resp.). Origin of HA-tagged RPS6KB1 (provided by J.Blenis, Harvard Medical School) according Addgene. (De)phosphorylation sites in RPS6KB1 have been mapped to the UniProt canonical form as it is present in different isoforms and the identiy of the transfected isform cannot be established. tianyan.gao@uky.edu tianyan@uky.edu Accepted 2015-JUN-15 by ORCHARD Molecular and cellular biology 2011 IMEx mimix curation eif3b_mouse Eukaryotic translation initiation factor 3 subunit B Eif3b eIF3 p116 Eif3s9 Eukaryotic translation initiation factor 3 subunit 9 eIF-3-eta protein protein mouse Mus musculus Mouse MQDAENVAVPEAAEERAEPARQQPASESPPTDEAAGSGGSEVGQTEDAEEDAEAGPEPEVRAKPAAQSEEETATSPAASPTPQSAERSPSQEPSAPGKAEAVGEQARGHPSAGAEEEGGSDGSAAEAEPRALENGEADEPSFSDPEDFVDDVSEEELLGDVLKDRPQEADGIDSVIVVDNVPQVGPDRLEKLKNVIHKIFSKFGKIINDYYPEEDGKTKGYIFLEYASPAHAVDAVKNADGYKLDKQHTFRVNLFTDFDKYMTISDEWDIPEKQPFKDLGNLRYWLEEAECRDQYSVIFESGDRTSIFWNDVKDPVSIEERARWTETYVRWSPKGTYLATFHQRGIALWGGDKFKQIQRFSHQGVQLIDFSPCERYLVTFSPLMDTQDDPQAIIIWDILTGHKKRGFHCESSAHWPIFKWSHDGKFFARMTLDTLSIYETPSMGLLDKKSLKISGIKDFSWSPGGNIIAFWVPEDKDIPARVTLMQLPTRQEIRVRNLFNVVDCKLHWQKNGDYLCVKVDRTPKGTQGVVTNFEIFRMREKQVPVDVVEMKETIIAFAWEPNGSKFAVLHGEAPRISVSFYHVKSNGKIELIKMFDKQQANTIFWSPQGQFVVLAGLRSMNGALAFVDTSDCTVMNIAEHYMASDVEWDPTGRYVVTSVSWWSHKVDNAYWLWTFQGRLLQKNNKDRFCQLLWRPRPPTLLSQDQIKQIKKDLKKYSKIFEQKDRLSQSKASKELVERRRTMMEDFRQYRKMAQELYMKQKNERLELRGGVDTDELDSNVDDWEEETIEFFVTEEVIPLGSQE D770AC70BFE9832F phlp2_human PH domain leucine-rich repeat-containing protein phosphatase 2 PHLPP2 KIAA0931 PHLPPL PH domain leucine-rich repeat-containing protein phosphatase-like protein protein human Homo sapiens Human MKRNGSRNCLNRRSRFGSRERDWLREDVKRGCVYLYGADTTTATTTTTTSSSSSSSSSSSDLHLVLCTVETPASEICAGEGRESLYLQLHGDLVRRLEPTERPLQIVYDYLSRLGFDDPVRIQEEATNPDLGCMIRFYGEKPCHMDRLDRILLSGIYNVRKGKTQLHKWAERLVVLCGTCLIVSSVKDCQTGKMHILPLVGGKIEEVKRRQYSLAFSSAGAQAQTYHVSFETLAEYQRWQRQASKVVSQRISTVDLSCYSLEEVPEHLFYSQDITYLNLRHNFMQLERPGGLDTLYKFSQLKGLNLSHNKLGLFPILLCEISTLTELNLSCNGFHDLPSQIGNLLNLQTLCLDGNFLTTLPEELGNLQQLSSLGISFNNFSQIPEVYEKLTMLDRVVMAGNCLEVLNLGVLNRMNHIKHVDLRMNHLKTMVIENLEGNKHITHVDLRDNRLTDLDLSSLCSLEQLHCGRNQLRELTLSGFSLRTLYASSNRLTAVNVYPVPSLLTFLDLSRNLLECVPDWACEAKKIEVLDVSYNLLTEVPVRILSSLSLRKLMLGHNHVQNLPTLVEHIPLEVLDLQHNALTRLPDTLFSKALNLRYLNASANSLESLPSACTGEESLSMLQLLYLTNNLLTDQCIPVLVGHLHLRILHLANNQLQTFPASKLNKLEQLEELNLSGNKLKTIPTTIANCKRLHTLVAHSNNISIFPEILQLPQIQFVDLSCNDLTEILIPEALPATLQDLDLTGNTNLVLEHKTLDIFSHITTLKIDQKPLPTTDSTVTSTFWSHGLAEMAGQRNKLCVSALAMDSFAEGVGAVYGMFDGDRNEELPRLLQCTMADVLLEEVQQSTNDTVFMANTFLVSHRKLGMAGQKLGSSALLCYIRPDTADPASSFSLTVANVGTCQAVLCRGGKPVPLSKVFSLEQDPEEAQRVKDQKAIITEDNKVNGVTCCTRMLGCTYLYPWILPKPHISSTPLTIQDELLILGNKALWEHLSYTEAVNAVRHVQDPLAAAKKLCTLAQSYGCQDNVGAMVVYLNIGEEGCTCEMNGLTLPGPVGFASTTTIKDAPKPATPSSSSGIASEFSSEMSTSEVSSEVGSTASDEHNAGGLDTALLPRPERRCSLHPTPTSGLFQRQPSSATFSSNQSDNGLDSDDDQPVEGVITNGSKVEVEVDIHCCRGRDLENSPPLIESSPTLCSEEHARGSCFGIRRQNSVNSGMLLPMSKDRMELQKSPSTSCLYGKKLSNGSIVPLEDSLNLIEVATEVPKRKTGYFAAPTQMEPEDQFVVPHDLEEEVKEQMKQHQDSRLEPEPHEEDRTEPPEEFDTAL DIP protein Q6ZVD8 original sequence version: 104 04FC8550C2E22A6D phlp1_human PH domain leucine-rich repeat-containing protein phosphatase 1 PHLPP1 KIAA0606 PHLPP PLEKHE1 SCOP Pleckstrin homology domain-containing family E member 1 Suprachiasmatic nucleus circadian oscillatory protein protein protein human Homo sapiens Human MEPAAAATVQRLPELGREDRASAPAAAAAAAAAAAAAAAALAAAAGGGRSPEPALTPAAPSGGNGSGSGAREEAPGEAPPGPLPGRAGGAGRRRRRGAPQPIAGGAAPVPGAGGGANSLLLRRGRLKRNLSAAAAAASSSSSSSAAAASHSPGAAGLPASCSASASLCTRSLDRKTLLLKHRQTLQLQPSDRDWVRHQLQRGCVHVFDRHMASTYLRPVLCTLDTTAGEVAARLLQLGHKGGGVVKVLGQGPGAAAAREPAEPPPEAGPRLAPPEPRDSEVPPARSAPGAFGGPPRAPPADLPLPVGGPGGWSRRASPAPSDSSPGEPFVGGPVSSPRAPRPVVSDTESFSLSPSAESVSDRLDPYSSGGGSSSSSEELEADAASAPTGVPGQPRRPGHPAQPLPLPQTASSPQPQQKAPRAIDSPGGAVREGSCEEKAAAAVAPGGLQSTPGRSGVTAEKAPPPPPPPTLYVQLHGETTRRLEAEEKPLQIQNDYLFQLGFGELWRVQEEGMDSEIGCLIRFYAGKPHSTGSSERIQLSGMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSLTGKMHVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQNPSLPAARGLNELQRFTKLKSLNLSNNHLGDFPLAVCSIPTLAELNVSCNALRSVPAAVGVMHNLQTFLLDGNFLQSLPAELENMKQLSYLGLSFNEFTDIPEVLEKLTAVDKLCMSGNCVETLRLQALRKMPHIKHVDLRLNVIRKLIADEVDFLQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLDICGYFLKALYASSNELVQLDVYPVPNYLSYMDVSRNRLENVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNQLARLPERLERTSVEVLDVQHNQLLELPPNLLMKADSLRFLNASANKLESLPPATLSEETNSILQELYLTNNSLTDKCVPLLTGHPHLKILHMAYNRLQSFPASKMAKLEELEEIDLSGNKLKAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEIKCVDLSCNELSEVTLPENLPPKLQELDLTGNPRLVLDHKTLELLNNIRCFKIDQPSTGDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFCDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEEEYMVNTFIVMQRKLGTAGQKLGGAAVLCHIKHDPVDPGGSFTLTSANVGKCQTVLCRNGKPLPLSRSYIMSCEEELKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWDSLSVEEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSFCCCELSAGGAVPPPSPGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISSELSTSEMSSEVGSTASDEPPPGALSENSPAYPSEQRCMLHPICLSNSFQRQLSSATFSSAFSDNGLDSDDEEPIEGVFTNGSRVEVEVDIHCSRAKEKEKQQHLLQVPAEASDEGIVISANEDEPGLPRKADFSAVGTIGRRRANGSVAPQERSHNVIEVATDAPLRKPGGYFAAPAQPDPDDQFIIPPELEEEVKEIMKHHQEQQQQQQPPPPPQLQPQLPRHYQLDQLPDYYDTPL A4AB53F334EA8AB6 ks6b1_rat Ribosomal protein S6 kinase beta-1 Rps6kb1 Ribosomal protein S6 kinase I 70 kDa ribosomal protein S6 kinase 1 p70 ribosomal S6 kinase alpha protein protein rat Rattus norvegicus (Rat) Rat MRRRRRRDGFYPAPDFRHREAEDMAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTTSGEASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL 013BA13EFB3508D7 eif3b_human Eukaryotic translation initiation factor 3 subunit B Eukaryotic translation initiation factor 3 subunit 9 eIF-3-eta eIF3 p116 eIF3 p110 Prt1 homolog EIF3B EIF3S9 protein protein human Homo sapiens Human MQDAENVAVPEAAEERAEPGQQQPAAEPPPAEGLLRPAGPGAPEAAGTEASSEEVGIAEAGPESEVRTEPAAEAEAASGPSESPSPPAAEELPGSHAEPPVPAQGEAPGEQARDERSDSRAQAVSEDAGGNEGRAAEAEPRALENGDADEPSFSDPEDFVDDVSEEELLGDVLKDRPQEADGIDSVIVVDNVPQVGPDRLEKLKNVIHKIFSKFGKITNDFYPEEDGKTKGYIFLEYASPAHAVDAVKNADGYKLDKQHTFRVNLFTDFDKYMTISDEWDIPEKQPFKDLGNLRYWLEEAECRDQYSVIFESGDRTSIFWNDVKDPVSIEERARWTETYVRWSPKGTYLATFHQRGIALWGGEKFKQIQRFSHQGVQLIDFSPCERYLVTFSPLMDTQDDPQAIIIWDILTGHKKRGFHCESSAHWPIFKWSHDGKFFARMTLDTLSIYETPSMGLLDKKSLKISGIKDFSWSPGGNIIAFWVPEDKDIPARVTLMQLPTRQEIRVRNLFNVVDCKLHWQKNGDYLCVKVDRTPKGTQGVVTNFEIFRMREKQVPVDVVEMKETIIAFAWEPNGSKFAVLHGEAPRISVSFYHVKNNGKIELIKMFDKQQANTIFWSPQGQFVVLAGLRSMNGALAFVDTSDCTVMNIAEHYMASDVEWDPTGRYVVTSVSWWSHKVDNAYWLWTFQGRLLQKNNKDRFCQLLWRPRPPTLLSQEQIKQIKKDLKKYSKIFEQKDRLSQSKASKELVERRRTMMEDFRKYRKMAQELYMEQKNERLELRGGVDTDELDSNVDDWEEETIEFFVTEEIIPLGNQE DIP protein P55884 original sequence version: 84 C687A986EDAE0F5E ks6b1_human Ribosomal protein S6 kinase beta-1 RPS6KB1 STK14A Ribosomal protein S6 kinase I p70 ribosomal S6 kinase alpha 70 kDa ribosomal protein S6 kinase 1 Serine/threonine-protein kinase 14A protein protein human Homo sapiens Human MRRRRRRDGFYPAPDFRDREAEDMAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL DIP protein P23443 original sequence version: 163 DIP protein P23443 original sequence version: 88 2C3BA13CCDAF4AB3 ks6b1_mouse Ribosomal protein S6 kinase beta-1 Ribosomal protein S6 kinase I p70 ribosomal S6 kinase alpha 70 kDa ribosomal protein S6 kinase 1 Rps6kb1 protein protein mouse Mus musculus Mouse MRRRRRRDGFYLAPDFRHREAEDMAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTVSGEASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL 5B3C09F6BB365EE2 rs6_mouse 40S ribosomal protein S6 Rps6 Phosphoprotein NP33 protein protein mouse Mus musculus Mouse MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK DIP protein P62754 original sequence version: 64 A61E435884E636AE rps6kb1-phlpp1-2 1 11 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey association association 5A rps6kb1-phlpp1-1 3 11 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey association association 5A phlpp1-rps6kb1-1 2 9 enzyme target enzyme target substrate substrate neutral component neutral component possible phosphosthr-252 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain possible phosphosthr-412 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 8 enzyme enzyme neutral component neutral component ppmtype_phosphatase_dom sufficient to bind sufficient binding region sufficient to bind range range range range dephosphorylation dephosphorylation reaction dephosphorylation 5B eif3b-rps6kb1-1 1 11 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association 6B Association decreased upon knockdown of PHLPP1 or PHLPP2. eif3b-rps6kb1-2 5 12 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 6C The association is decreased upon knock down of Phlpp1 rps6kb1-rps6kb1-1 4 9 enzyme enzyme neutral component neutral component human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation 5B rps6-rps6kb1-1 4 13 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 enzyme enzyme neutral component neutral component human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation 5B