IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells. Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells. Molecular and cellular biology 2009 imex curation Banumathy G., Somaiah N., Zhang R., Tang Y., Hoffmann J., Andrake M., Ceulemans H., Schultz D., Marmorstein R., Adams PD. Source of ASF1A taken from previous work of the author (PMID:15621527) p.adams@beatson.gla.ac.uk 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-imr_90 Homo sapiens embryonic fetal lung fibroblast imr_90 human, Caucasian female 16 fetal weeks; Tissue: lung, embryonic fibroblast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Banumathy G., Somaiah N., Zhang R., Tang Y., Hoffmann J., Andrake M., Ceulemans H., Schultz D., Marmorstein R., Adams PD. Source of ASF1A taken from previous work of the author (PMID:15621527) p.adams@beatson.gla.ac.uk ku.ca.alg.nostaeb@smada.p Accepted 2015-NOV-26 AT 14:34 GMT AT 14:34 GMT by PPORRAS Molecular and cellular biology 2009 Only protein-protein interactions imex curation imex curation The proteins were pre-purified on His (IMAC) resin and I had added 'purified' as experimental preparation so I think the direct stands. Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells. Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells. Molecular and cellular biology 2009 imex curation Banumathy G., Somaiah N., Zhang R., Tang Y., Hoffmann J., Andrake M., Ceulemans H., Schultz D., Marmorstein R., Adams PD. Source of ASF1A taken from previous work of the author (PMID:15621527) p.adams@beatson.gla.ac.uk 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-imr_90 Homo sapiens embryonic fetal lung fibroblast imr_90 human, Caucasian female 16 fetal weeks; Tissue: lung, embryonic fibroblast ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Banumathy G., Somaiah N., Zhang R., Tang Y., Hoffmann J., Andrake M., Ceulemans H., Schultz D., Marmorstein R., Adams PD. Source of ASF1A taken from previous work of the author (PMID:15621527) p.adams@beatson.gla.ac.uk ku.ca.alg.nostaeb@smada.p Accepted 2015-NOV-26 AT 14:34 GMT AT 14:34 GMT by PPORRAS Molecular and cellular biology 2009 Only protein-protein interactions imex curation imex curation The proteins were pre-purified on His (IMAC) resin and I had added 'purified' as experimental preparation so I think the direct stands. Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells. Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells. Molecular and cellular biology 2009 imex curation Banumathy G., Somaiah N., Zhang R., Tang Y., Hoffmann J., Andrake M., Ceulemans H., Schultz D., Marmorstein R., Adams PD. Source of ASF1A taken from previous work of the author (PMID:15621527) p.adams@beatson.gla.ac.uk 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-mpc Human mesenchymal progenitor cell Human mpc Megenchymal pronitor cell (MPC) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Banumathy G., Somaiah N., Zhang R., Tang Y., Hoffmann J., Andrake M., Ceulemans H., Schultz D., Marmorstein R., Adams PD. Source of ASF1A taken from previous work of the author (PMID:15621527) p.adams@beatson.gla.ac.uk ku.ca.alg.nostaeb@smada.p Accepted 2015-NOV-26 AT 14:34 GMT AT 14:34 GMT by PPORRAS Molecular and cellular biology 2009 Only protein-protein interactions imex curation imex curation The proteins were pre-purified on His (IMAC) resin and I had added 'purified' as experimental preparation so I think the direct stands. Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells. Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells. Molecular and cellular biology 2009 imex curation Banumathy G., Somaiah N., Zhang R., Tang Y., Hoffmann J., Andrake M., Ceulemans H., Schultz D., Marmorstein R., Adams PD. Source of ASF1A taken from previous work of the author (PMID:15621527) p.adams@beatson.gla.ac.uk 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-wi_38 Homo sapiens Caucasian fetal lung fibroblast cell line wi_38 WI39 human Caucasian fetal lung fibroblast cell line From normal embryonic lung of female. Have very broad human virus spectra, especially useful for isolation of rhinoviruses. Cells form multilayered membrane when held for long periods at 37C with periodic pH adjustments. Used in preparation of human virus vaccines. 2n = 46, diploid except at high passage number anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Banumathy G., Somaiah N., Zhang R., Tang Y., Hoffmann J., Andrake M., Ceulemans H., Schultz D., Marmorstein R., Adams PD. Source of ASF1A taken from previous work of the author (PMID:15621527) p.adams@beatson.gla.ac.uk ku.ca.alg.nostaeb@smada.p Accepted 2015-NOV-26 AT 14:34 GMT AT 14:34 GMT by PPORRAS Molecular and cellular biology 2009 Only protein-protein interactions imex curation imex curation The proteins were pre-purified on His (IMAC) resin and I had added 'purified' as experimental preparation so I think the direct stands. Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells. Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells. Molecular and cellular biology 2009 imex curation Banumathy G., Somaiah N., Zhang R., Tang Y., Hoffmann J., Andrake M., Ceulemans H., Schultz D., Marmorstein R., Adams PD. Source of ASF1A taken from previous work of the author (PMID:15621527) p.adams@beatson.gla.ac.uk 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Banumathy G., Somaiah N., Zhang R., Tang Y., Hoffmann J., Andrake M., Ceulemans H., Schultz D., Marmorstein R., Adams PD. Source of ASF1A taken from previous work of the author (PMID:15621527) p.adams@beatson.gla.ac.uk ku.ca.alg.nostaeb@smada.p Accepted 2015-NOV-26 AT 14:34 GMT AT 14:34 GMT by PPORRAS Molecular and cellular biology 2009 Only protein-protein interactions imex curation imex curation The proteins were pre-purified on His (IMAC) resin and I had added 'purified' as experimental preparation so I think the direct stands. Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells. Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells. Molecular and cellular biology 2009 imex curation Banumathy G., Somaiah N., Zhang R., Tang Y., Hoffmann J., Andrake M., Ceulemans H., Schultz D., Marmorstein R., Adams PD. Source of ASF1A taken from previous work of the author (PMID:15621527) p.adams@beatson.gla.ac.uk 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Banumathy G., Somaiah N., Zhang R., Tang Y., Hoffmann J., Andrake M., Ceulemans H., Schultz D., Marmorstein R., Adams PD. Source of ASF1A taken from previous work of the author (PMID:15621527) p.adams@beatson.gla.ac.uk ku.ca.alg.nostaeb@smada.p Accepted 2015-NOV-26 AT 14:34 GMT AT 14:34 GMT by PPORRAS Molecular and cellular biology 2009 Only protein-protein interactions imex curation imex curation The proteins were pre-purified on His (IMAC) resin and I had added 'purified' as experimental preparation so I think the direct stands. Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells. Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells. Molecular and cellular biology 2009 imex curation Banumathy G., Somaiah N., Zhang R., Tang Y., Hoffmann J., Andrake M., Ceulemans H., Schultz D., Marmorstein R., Adams PD. Source of ASF1A taken from previous work of the author (PMID:15621527) p.adams@beatson.gla.ac.uk 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Banumathy G., Somaiah N., Zhang R., Tang Y., Hoffmann J., Andrake M., Ceulemans H., Schultz D., Marmorstein R., Adams PD. Source of ASF1A taken from previous work of the author (PMID:15621527) p.adams@beatson.gla.ac.uk ku.ca.alg.nostaeb@smada.p Accepted 2015-NOV-26 AT 14:34 GMT AT 14:34 GMT by PPORRAS Molecular and cellular biology 2009 Only protein-protein interactions imex curation imex curation The proteins were pre-purified on His (IMAC) resin and I had added 'purified' as experimental preparation so I think the direct stands. Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells. Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells. Molecular and cellular biology 2009 imex curation Banumathy G., Somaiah N., Zhang R., Tang Y., Hoffmann J., Andrake M., Ceulemans H., Schultz D., Marmorstein R., Adams PD. Source of ASF1A taken from previous work of the author (PMID:15621527) p.adams@beatson.gla.ac.uk 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Banumathy G., Somaiah N., Zhang R., Tang Y., Hoffmann J., Andrake M., Ceulemans H., Schultz D., Marmorstein R., Adams PD. Source of ASF1A taken from previous work of the author (PMID:15621527) p.adams@beatson.gla.ac.uk ku.ca.alg.nostaeb@smada.p Accepted 2015-NOV-26 AT 14:34 GMT AT 14:34 GMT by PPORRAS Molecular and cellular biology 2009 Only protein-protein interactions imex curation imex curation The proteins were pre-purified on His (IMAC) resin and I had added 'purified' as experimental preparation so I think the direct stands. hira_human Protein HIRA HIRA TUP1-like enhancer of split protein 1 DGCR1 HIR TUPLE1 protein protein human Homo sapiens Human MKLLKPTWVNHNGKPIFSVDIHPDGTKFATGGQGQDSGKVVIWNMSPVLQEDDEKDENIPKMLCQMDNHLACVNCVRWSNSGMYLASGGDDKLIMVWKRATYIGPSTVFGSSGKLANVEQWRCVSILRNHSGDVMDVAWSPHDAWLASCSVDNTVVIWNAVKFPEILATLRGHSGLVKGLTWDPVGKYIASQADDRSLKVWRTLDWQLETSITKPFDECGGTTHVLRLSWSPDGHYLVSAHAMNNSGPTAQIIEREGWKTNMDFVGHRKAVTVVKFNPKIFKKKQKNGSSAKPSCPYCCCAVGSKDRSLSVWLTCLKRPLVVIHELFDKSIMDISWTLNGLGILVCSMDGSVAFLDFSQDELGDPLSEEEKSRIHQSTYGKSLAIMTEAQLSTAVIENPEMLKYQRRQQQQQLDQKSAATREMGSATSVAGVVNGESLEDIRKNLLKKQVETRTADGRRRITPLCIAQLDTGDFSTAFFNSIPLSGSLAGTMLSSHSSPQLLPLDSSTPNSFGASKPCTEPVVAASARPAGDSVNKDSMNATSTPAALSPSVLTTPSKIEPMKAFDSRFTERSKATPGAPALTSMTPTAVERLKEQNLVKELRPRDLLESSSDSDEKVPLAKASSLSKRKLELEVETVEKKKKGRPRKDSRLMPVSLSVQSPAALTAEKEAMCLSAPALALKLPIPSPQRAFTLQVSSDPSMYIEVENEVTVVGGVKLSRLKCNREGKEWETVLTSRILTAAGSCDVVCVACEKRMLSVFSTCGRRLLSPILLPSPISTLHCTGSYVMALTAAATLSVWDVHRQVVVVKEESLHSILAGSDMTVSQILLTQHGIPVMNLSDGKAYCFNPSLSTWNLVSDKQDSLAQCADFRSSLPSQDAMLCSGPLAIIQGRTSNSGRQAARLFSVPHVVQQETTLAYLENQVAAALTLQSSHEYRHWLLVYARYLVNEGFEYRLREICKDLLGPVHYSTGSQWESTVVGLRKRELLKELLPVIGQNLRFQRLFTECQEQLDILRDK DIP protein P54198 original sequence version: 124 DIP protein P54198 original sequence version: 66 475784B2FF9D16D6 hpc2_yeast Histone promoter control protein 2 YBR1503 YBR215W HPC2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDQKAIVLDNSKSGSKQTKSSGKMQTQTDTNAEVLNTDNSIKKETGSDSEDLFNKFSNKKTNRKIPNIAEELAKNRNYVKGASPSPIIISGSSSTSPSGPSSSSTNPMGIPTNRFNKNTVELYQHSPSPVMTTNKTDTEEKRQNNRNMDNKNTPERGSSSFAAKQLKISSLLTISSNEDSKTLHINDTNGNKNSNAASNNIPSAYAELHTEGNSIESLIKPPSSPRNKSLTPKVILPTQNMDGTIAKDPHLGDNTPGILIAKTSSPVNLDVESTAQSLGKFNKSTNSLKAALTKAPAEKVSLKRSISSVTNSDSNISSSKKPTSEKAKKSSSASAILPKPTTTKTSKKAASNSSDSTRKKNASNKTTSAIKKESNAGSKLNTVKKENSSLSSIKATEKEKDKGGNSTEAKNSTSNVRKEPTAKSPKRLVAAPTVSPPKILQTAETKAKEPSILIDVPLYQADTNDYLDENGQVIFNLSTLIKEKYHPKSKELAQLKDSKRNLLMQLSDHSNGSLEKEKDEEGDVIELDDDEDMEEDEGEIDTETNTVTTTISPKKKSHPMKGKNLIGKYDVEDPFIDDSELLWEEQRAATKDGFFVYFGPLIEKGHYASLERANGTMKRGGVKNK 9F787D6EA37A8374 ubn1_human Ubinuclein-1 UBN1 HIRA-binding protein Protein VT4 Ubiquitously expressed nuclear protein protein protein human Homo sapiens Human MSEPHRVQFTSLPGSLNPAFLKKSRKEEAGAGEQHQDCEPAAAAVRITLTLFEPDHKRCPEFFYPELVKNIRGKVKGLQPGDKKKDLSDPFNDEEKERHKVEALARKFEEKYGGKKRRKDRIQDLIDMGYGYDESDSFIDNSEAYDELVPASLTTKYGGFYINSGTLQFRQASESEDDFIKEKKKKSPKKRKLKEGGEKIKKKKKDDTYDKEKKSKKSKFSKAGFTALNASKEKKKKKYSGALSVKEMLKKFQKEKEAQKKREEEHKPVAVPSAEAQGLRELEGASDPLLSLFGSTSDNDLLQAATAMDSLTDLDLEHLLSESPEGSPFRDMDDGSDSLGVGLDQEFRQPSSLPEGLPAPLEKRVKELAQAARAAEGESRQKFFTQDINGILLDIEAQTRELSSQVRSGVYAYLASFLPCSKDALLKRARKLHLYEQGGRLKEPLQKLKEAIGRAMPEQMAKYQDECQAHTQAKVAKMLEEEKDKEQRDRICSDEEEDEEKGGRRIMGPRKKFQWNDEIRELLCQVVKIKLESQDLERNNKAQAWEDCVKGFLDAEVKPLWPKGWMQARTLFKESRRGHGHLTSILAKKKVMAPSKIKVKESSTKPDKKVSVPSGQIGGPIALPSDHQTGGLSIGASSRELPSQASGGLANPPPVNLEDSLDEDLIRNPASSVEAVSKELAALNSRAAGNSEFTLPAPSKAPAEKVGGVLCTEEKRNFAKPSPSAPPPASSLQSPLNFLAEQALALGQSSQEKKPESSGYKELSCQAPLNKGLPEVHQSKAKHHSLPRTSHGPQVAVPVPGPQVKVFHAGTQQQKNFTPPSPFANKLQGPKASPTQCHRSLLQLVKTAAKGQGFHPSAPATSGGLSASSSSSHKTPASSSSALSHPAKPHSVSSAGSSYKNNPFASSISKHGVSSGSSSSGGTPVQSSVSGSLVPGIQPPSVGQATSRPVPSSAGKKMPVSQKLTLVAPPGGPNGDSSGGTQGVAKLLTSPSLKPSAVSSVTSSTSLSKGASGTVLLAGSSLMASPYKSSSPKLSGAMSSNSLGIITPVPIPVHVLSFSADSSAKAGVSKDAIVTGPAPGSFHHGLGHSLLAGLHSSPPHAAPLPHAAVPTHIPQSLPGASQLHGKGPAVPRKL 8DE68C63D4E8E01B asf1a_human Histone chaperone ASF1A Anti-silencing function protein 1 homolog A CCG1-interacting factor A ASF1A CGI-98 HSPC146 protein protein human Homo sapiens Human MAKVQVNNVVVLDNPSPFYNPFQFEITFECIEDLSEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRHMFVFQADAPNPGLIPDADAVGVTVVLITCTYRGQEFIRVGYYVNNEYTETELRENPPVKPDFSKLQRNILASNPRVTRFHINWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCM DIP protein Q9Y294 original sequence version: 111 DIP protein Q9Y294 original sequence version: 131 DIP protein Q9Y294 original sequence version: 106 This sequence has been withdrawn from Uniprot. 9819D531D3A9FC68 ubn2_human Ubinuclein-2 UBN2 KIAA2030 protein protein human Homo sapiens Human MAEPRRVAFISLSPVRRREAEYPGPEREPEYPREPPRLEPQPYREPARAEPPAPREPAPRSDAQPPSREKPLPQREVSRAEPPMSLQREPPRPEPPPPFPPLPLQPPPPRESASRAEQPPRPPRETVRLELVLKDPTDESCVEFSYPELLLCGEQRKKLIHTEDPFNDEHQERQEVEMLAKKFEMKYGGKPRKHRKDRLQDLIDIGFGYDETDPFIDNSEAYDELVPASLTTKYGGFYINTGTLQFRQASDTEEDDITDNQKHKPPKVPKIKEDDIEMKKRKRKEEGEKEKKPRKKVPKQLGVVALNSHKSEKKKKRYKDSLSLAAMIRKFQKEKDALKKESNPKVPVTLSTPSLNKPPCAAAALGNDVPDLNLSSGDPDLPIFVSTNEHELFQEAENALEMLDDFDFDRLLDAASDGSPLSESGGENGTTTQPTYTSQVMPKVVPTLPEGLPVLLEKRIEDLRVAAKLFDEEGRKKFFTQDMNNILLDIELQLQELGPVIRSGVYSHLEAFVPCNKETLVKRLKKLHLNVQDDRLREPLQKLKLAVSNVMPEQLFKYQEDCQARSQAKCAKLQTDEEREKNGSEEDDDEKPGKRVIGPRKKFHWDDTIRTLLCNLVEIKLGCYELEPNKSQSAEDYLKSFMETEVKPLWPKGWMQARMLFKESRSVHNHLTSAPAKKKVIPAPKPKVKEVMVKTLPLHSFPTMLKECSPKKDQKTPTSLVASVSGPPTSSSTAAIAAASSSSAPAQETICLDDSLDEDLSFHSPSLDLVSEALAVINNGNKGPPVGSRISMPTTKPRPGLREEKLASIMSKLPLATPKKLDSTQTTHSSSLIAGHTGPVPKKPQDLAHTGISSGLIAGSSIQNPKVSLEPLPARLLQQGLQRSSQIHTSSSSQTHVSSSSQAQIAASSHALGTSEAQDASSLTQVTKVHQHSAVQQNYVSPLQATISKSQTNPVVKLSNNPQLSCSSSLIKTSDKPLMYRLPLSTPSPGNGSQGSHPLVSRTVPSTTTSSNYLAKAMVSQISTQGFKSPFSMAASPKLAASPKPATSPKPLPSPKPSASPKPSLSAKPSVSTKLISKSNPTPKPTVSPSSSSPNALVAQGSHSSTNSPVHKQPSGMNISRQSPTLNLLPSSRTSGLPPTKNLQAPSKLTNSSSTGTVGKNSLSGIAMNVPASRGSNLNSSGANRTSLSGGTGSGTQGATKPLSTPHRPSTASGSSVVTASVQSTAGASLLANASPLTLMTSPLSVTNQNVTPFGMLGGLVPVTMPFQFPLEIFGFGTDTAGVTTTSGSTSAAFHHSLTQNLLKGLQPGGAQHAATLSHSPLPAHLQQAFHDGGQSKGDTKLPRKSQ 6B52672B860B18B5 hir1_yeast Protein HIR1 HIR1 YBL008W YBL0318 Histone transcription regulator 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKVVKFPWLAHREESRKYEIYTVDVSHDGKRLATGGLDGKIRIWSIDSILRCMELESLTPEIPLPQDLQMPLCSMSRHTGSITCVKFSPDGKYLASGSDDRILLIWALDEEQSSQPAFGSEHEREHWTVRKRLVAHDNDIQDICWAPDSSILVTVGLDRSVIVWNGSTFEKLKRFDVHQSLVKGVVFDPANKYFATTSDDRTMKIFRYHKTGDISFTIEHIITEPFKESPLTTYFRRPSWSPDGQHIAVPNATNGPVSSVAIVNRGTWDTNVSLIGHDAPTEVARFNPRLFERNAGVKQKKDDDPENALVGQNDDKVHHFDKNIDSVVATAGQDKSLAVWSTSRPRPILVAFDIANKSITDMSWNPDGSLLFVASLDSSITLFKFENNELGKPIPLEKNMEQLYRYGVDKDSLDFPESINQLLLEDQTKSFKHTKISTSKLGENHPTLATNSASNQKDNNDASVSRSEHINILIPKRKKDAILNKAVTLKSGKKRVAPTLISTSSSSPFSNGIKKPTLDSKRIENNVKSSTKTINSKNTLLNVPEGVEKKISISSFPLPRLGIHSLIMGTKERSAWKISNSELENDDADNAGGKGSDGTSNSIDDIAVLSEEENDFHRMTLNAKLTQEKIWSEEPTTRCLLQSDVIPDTDVVVLEGGSLDDIAVLEIRNGVERSIQFDSEALLDNPTRILGYQGGKRTIETFIPEVIICAIGSKDCKCWCLASANGSIYILSYNGQQRIPKICLGHKVIKMVTSSKYLLVLTERGLFFAWDLLDLKLVLRNVPILPILNGQPIHGNKVRINKVIKCFRLDGSSCDLLLEVGDPKNVYKWTKDLGCWSLYK 40A2932F3306358A 40A2932F3306358A ccna2_human_gene Human CCNA2 gene gene gene human Homo sapiens Human ubn1-asf1a-1 1 11 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey association association Fig. 1E ubn1-asf1a-2 4 11 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey association association Fig. 1F hira-asf1a-3 3 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait association association Fig. 1F ubn1-asf1a-4 5 11 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey association association Fig. 1F hira-asf1a-1 8 12 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 9 unspecified role unspecified role bait bait N/A required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain P54198:p.Arg227Lys mutation mutation certain certain sequence position certain certain certain sequence position certain R K Wed May 02 16:18:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg227lys' Tue Jun 30 17:12:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg227Lys' in vitro In vitro physical association physical association Fig. 3A, 5D hira-ubn1-1 8 11 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 9 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined wd40 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range in vitro In vitro physical association physical association Fig. 2B, 3A ubn1-hira-1 8 11 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9NPG3:p.Phe138_Asp140delinsGlyGlyLeu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain FID GGL Fri Jul 22 11:05:58 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'phe138glu_ile139glu_asp140leu' Wed May 02 19:55:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe_ile_asp138-140gly_gly_leu' Tue Jun 30 18:12:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe138_Asp140delinsGlyGlyLeu' region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q9NPG3:p.[Phe160Glu;Ile162Glu] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F E certain certain sequence position certain certain certain sequence position certain I E Wed May 02 20:19:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe160glu,ile162glu' Tue Jun 30 17:25:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe160Glu;Ile162Glu]' Q9NPG3:p.Phe160Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F E Wed May 02 18:20:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe160glu' Tue Jun 30 18:01:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe160Glu' Q9NPG3:p.Ile162Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I E Wed May 02 14:54:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile162glu' Tue Jun 30 17:22:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile162Glu' in vitro In vitro 9 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined wd40 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P54198:p.Arg227Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R K Wed May 02 18:03:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg227lys' Tue Jun 30 18:40:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg227Lys' in vitro In vitro 60 52 physical association physical association Fig.3B, 5B, 5C, 5E ubn2-hira-1 8 13 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 9 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined wd40 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P54198:p.Arg227Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R K Wed May 02 15:05:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg227lys' Tue Jun 30 18:51:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg227Lys' in vitro In vitro 69 66 physical association physical association Fig. 3B, 5E ubn1-hira-2 7 11 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined wd40 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 77 74 direct interaction direct interaction Fig.4A hir1-hpc2-1 6 10 unspecified role unspecified role prey prey hrd region required to bind necessary binding region required to bind range range range range 14 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 83 80 physical association physical association Fig. 4B ccna2_human_gene-ubn1-1 2 15 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait association association Fig. 7A ubn1-ccna2_human_gene-1 2 15 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait N/A ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 7B asf1a-ccna2_human_gene-1 2 15 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait association association Fig. 7E asf1a-hira-1 1 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey association association Fig. 1E hira-asf1a-2 4 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait association association Fig. 1F hira-asf1a-4 5 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait association association Fig. 1F hira-asf1a-5 8 11 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 12 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 9 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain in vitro In vitro association association Fig. 3A, 5D