DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The Salmonella virulence protein SifA is a G protein antagonist. The Salmonella virulence protein SifA is a G protein antagonist. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Jackson LK., Nawabi P., Hentea C., Roark EA., Haldar K. khaldar@nd.edu Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Jackson LK., Nawabi P., Hentea C., Roark EA., Haldar K. khaldar@nd.edu Accepted 2015-MAY-06 by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation The Salmonella virulence protein SifA is a G protein antagonist. The Salmonella virulence protein SifA is a G protein antagonist. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Jackson LK., Nawabi P., Hentea C., Roark EA., Haldar K. khaldar@nd.edu Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Jackson LK., Nawabi P., Hentea C., Roark EA., Haldar K. khaldar@nd.edu Accepted 2015-MAY-06 by ORCHARD Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation rab9a_human Ras-related protein Rab-9A RAB9A RAB9 protein protein human Homo sapiens Human MAGKSSLFKVILLGDGGVGKSSLMNRYVTNKFDTQLFHTIGVEFLNKDLEVDGHFVTMQIWDTAGQERFRSLRTPFYRGSDCCLLTFSVDDSQSFQNLSNWKKEFIYYADVKEPESFPFVILGNKIDISERQVSTEEAQAWCRDNGDYPYFETSAKDATNVAAAFEEAVRRVLATEDRSDHLIQTDTVNLHRKPKPSSSCC DIP protein P51151 original sequence version: 89 DIP protein P51151 original sequence version: 165 65B502C21E97DB72 sifa_salty Secreted effector protein SifA STM1224 sifA protein protein salty Salmonella typhimurium MPITIGNGFLKSEILTNSPRNTKEAWWKVLWEKIKDFFFSTGKAKADRCLHEMLFAERAPTRERLTEIFFELKELACASQRDRFQVHNPHENDATIILRIMDQNEENELLRITQNTDTFSCEVMGNLYFLMKDRPDILKSHPQMTAMIKRRYSEIVDYPLPSTLCLNPAGAPILSVPLDNIEGYLYTELRKGHLDGWKAQEKATYLAAKIQSGIEKTTRILHHANISESTQQNAFLETMAMCGLKQLEIPPPHTHIPIEKMVKEVLLADKTFQAFLVTDPSTSQSMLAEIVEAISDQVFHAIFRIDPQAIQKMAEEQLTTLHVRSEQQSGCLCCFL 83C6185148A774B9 83C6185148A774B9 rilp_human Rab-interacting lysosomal protein RILP PP10141 protein protein human Homo sapiens Human MEPRRAAPGVPGWGSREAAGSASAAELVYHLAGALGTELQDLARRFGPEAAAGLVPLVVRALELLEQAAVGPAPDSLQVSAQPAEQELRRLREENERLRRELRAGPQEERALLRQLKEVTDRQRDELRAHNRDLRQRGQETEALQEQLQRLLLVNAELRHKLAAMQTQLRAAQDRERERQQPGEAATPQAKERARGQAGRPGHQHGQEPEWATAGAGAPGNPEDPAEAAQQLGRPSEAGQCRFSREEFEQILQERNELKAKVFLLKEELAYFQRELLTDHRVPGLLLEAMKVAVRKQRKKIKAKMLGTPEEAESSEDEAGPWILLSDDKGDHPPPPESKIQSFFGLWYRGKAESSEDETSSPAPSKLGGEEEAQPQSPAPDPPCSALHEHLCLGASAAPEA 1057A1DAC2FFED94 1057A1DAC2FFED94 pkhm2_human Pleckstrin homology domain-containing family M member 2 KIAA0842 SKIP Salmonella-induced filaments A and kinesin-interacting protein PLEKHM2 protein protein human Homo sapiens Human MEPGEVKDRILENISLSVKKLQSYFAACEDEIPAIRNHDKVLQRLCEHLDHALLYGLQDLSSGYWVLVVHFTRREAIKQIEVLQHVATNLGRSRAWLYLALNENSLESYLRLFQENLGLLHKYYVKNALVCSHDHLTLFLTLVSGLEFIRFELDLDAPYLDLAPYMPDYYKPQYLLDFEDRLPSSVHGSDSLSLNSFNSVTSTNLEWDDSAIAPSSEDYDFGDVFPAVPSVPSTDWEDGDLTDTVSGPRSTASDLTSSKASTRSPTQRQNPFNEEPAETVSSSDTTPVHTTSQEKEEAQALDPPDACTELEVIRVTKKKKIGKKKKSRSDEEASPLHPACSQKKCAKQGDGDSRNGSPSLGRDSPDTMLASPQEEGEGPSSTTESSERSEPGLLIPEMKDTSMERLGQPLSKVIDQLNGQLDPSTWCSRAEPPDQSFRTGSPGDAPERPPLCDFSEGLSAPMDFYRFTVESPSTVTSGGGHHDPAGLGQPLHVPSSPEAAGQEEEGGGGEGQTPRPLEDTTREAQELEAQLSLVREGPVSEPEPGTQEVLCQLKRDQPSPCLSSAEDSGVDEGQGSPSEMVHSSEFRVDNNHLLLLMIHVFRENEEQLFKMIRMSTGHMEGNLQLLYVLLTDCYVYLLRKGATEKPYLVEEAVSYNELDYVSVGLDQQTVKLVCTNRRKQFLLDTADVALAEFFLASLKSAMIKGCREPPYPSILTDATMEKLALAKFVAQESKCEASAVTVRFYGLVHWEDPTDESLGPTPCHCSPPEGTITKEGMLHYKAGTSYLGKEHWKTCFVVLSNGILYQYPDRTDVIPLLSVNMGGEQCGGCRRANTTDRPHAFQVILSDRPCLELSAESEAEMAEWMQHLCQAVSKGVIPQGVAPSPCIPCCLVLTDDRLFTCHEDCQTSFFRSLGTAKLGDISAVSTEPGKEYCVLEFSQDSQQLLPPWVIYLSCTSELDRLLSALNSGWKTIYQVDLPHTAIQEASNKKKFEDALSLIHSAWQRSDSLCRGRASRDPWC DIP protein Q8IWE5 original sequence version: 33 DIP protein Q8IWE5 original sequence version: 97 FFD857F4C11BC0DB rab7a_human Ras-related protein Rab-7a RAB7A RAB7 protein protein human Homo sapiens Human MTSRKKVLLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVMVDDRLVTMQIWDTAGQERFQSLGVAFYRGADCCVLVFDVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAKASAESCSC DIP protein P51149 original sequence version: 113 DIP protein P51149 original sequence version: 92 DIP protein P51149 original sequence version: 162 A2AF33B16A672971 skip-rab9-1 1 3 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P51151:p.Gln66Leu mutation mutation certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 14:40:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln66leu' Tue Jun 30 20:21:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln66Leu' human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 6 unspecified role unspecified role prey prey N/A binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association 1A, 1C, 3A-D RAB9A was pre-loaded with guanosine 5'-[gamma-thio]triphosphate skip-sifa-1 1 4 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q56061:p.[Trp197Ala;Glu201Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain W A Fri Jul 22 10:58:43 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'glu201ala,trp197ala' Wed May 02 15:53:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp197ala,glu201ala' Tue Jun 30 18:46:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Trp197Ala;Glu201Ala]' ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey N/A binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association 1A, 2D, 3A-D rilp-rab7-1 1 7 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P51149:p.Gln67Leu mutation mutation certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 19:54:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln67leu' Tue Jun 30 18:05:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln67Leu' 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction 1B RAB7A was pre-loaded with guanosine 5'-[gamma-thio]triphosphate skip-rab9-2 2 6 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined N/A binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association 1D sifa-rab9-skip-1 1 3 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P51151:p.Gln66Leu mutation mutation certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 17:17:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln66leu' Wed May 02 18:41:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln66leu' Tue Jun 30 16:31:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln66Leu' 4 competitor competitor competitor unspecified role unspecified role ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey N/A binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association 2B rab9-skip-sifa-1 2 4 competitor competitor competitor unspecified role unspecified role 6 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined N/A binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association 2C