UniProt UniProt http://www.uniprot.org LEM2 is a novel MAN1-related inner nuclear membrane protein associated with A-type lamins. LEM2 is a novel MAN1-related inner nuclear membrane protein associated with A-type lamins. Journal of cell science 2005 imex curation Brachner A., Reipert S., Foisner R., Gotzmann J., Brachner A., Reipert S., Foisner R., Gotzmann J. Origin of lamin C accord. Dechet et al (2000a), PubMed 10984438. Origin of LAP2alpha accord. Vleck et all (1999), PubMed 10562549 cited by Dechet et al (2000a), PubMed 10984438 josef.gotzmann@meduniwien.ac.at 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Brachner A., Reipert S., Foisner R., Gotzmann J., Brachner A., Reipert S., Foisner R., Gotzmann J. Origin of lamin C accord. Dechet et al (2000a), PubMed 10984438. Origin of LAP2alpha accord. Vleck et all (1999), PubMed 10562549 cited by Dechet et al (2000a), PubMed 10984438 josef.gotzmann@meduniwien.ac.at Accepted 2015-AUG-03 by MGT Journal of cell science 2005 Only protein-protein interactions imex curation IMEx imex curation 1) lamin c: 10984438 does not give the spec directly but fig 7 depicts the domains and constructs used. the length of lamin A and lamin C isoform fit well with human (not mouse). coordinates would also fit with pig but for pig there is only genomic data with isoform prediction. And as you said 10984438 is citing refs linked to human. human shoud be fine then. 2) LAP2a: as i said i took it from Vleck et all (1999), PubMed 10562549 following Dechet et al (2000), PubMed 10984438. 10562549 is depicting LAP2A domains and constructs and says in fig 1: '...numbering according human....' with full length 693 aa which fits with uniprot human 694 aa including the cut off ini-met. (not the mouse). To me human is fine here. the data processing comment might be more explicit. LEM2 is a novel MAN1-related inner nuclear membrane protein associated with A-type lamins. LEM2 is a novel MAN1-related inner nuclear membrane protein associated with A-type lamins. Journal of cell science 2005 imex curation Brachner A., Reipert S., Foisner R., Gotzmann J., Brachner A., Reipert S., Foisner R., Gotzmann J. Origin of lamin C accord. Dechet et al (2000a), PubMed 10984438. Origin of LAP2alpha accord. Vleck et all (1999), PubMed 10562549 cited by Dechet et al (2000a), PubMed 10984438 josef.gotzmann@meduniwien.ac.at 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Brachner A., Reipert S., Foisner R., Gotzmann J., Brachner A., Reipert S., Foisner R., Gotzmann J. Origin of lamin C accord. Dechet et al (2000a), PubMed 10984438. Origin of LAP2alpha accord. Vleck et all (1999), PubMed 10562549 cited by Dechet et al (2000a), PubMed 10984438 josef.gotzmann@meduniwien.ac.at Accepted 2015-AUG-03 by MGT Journal of cell science 2005 Only protein-protein interactions imex curation IMEx imex curation 1) lamin c: 10984438 does not give the spec directly but fig 7 depicts the domains and constructs used. the length of lamin A and lamin C isoform fit well with human (not mouse). coordinates would also fit with pig but for pig there is only genomic data with isoform prediction. And as you said 10984438 is citing refs linked to human. human shoud be fine then. 2) LAP2a: as i said i took it from Vleck et all (1999), PubMed 10562549 following Dechet et al (2000), PubMed 10984438. 10562549 is depicting LAP2A domains and constructs and says in fig 1: '...numbering according human....' with full length 693 aa which fits with uniprot human 694 aa including the cut off ini-met. (not the mouse). To me human is fine here. the data processing comment might be more explicit. lemd2_human LEM domain-containing protein 2 LEMD2 protein protein human Homo sapiens Human MAGLSDLELRRELQALGFQPGPITDTTRDVYRNKLRRLRGEARLRDEERLREEARPRGEERLREEARLREDAPLRARPAAASPRAEPWLSQPASGSAYATPGAYGDIRPSAASWVGSRGLAYPARPAQLRRRASVRGSSEEDEDARTPDRATQGPGLAARRWWAASPAPARLPSSLLGPDPRPGLRATRAGPAGAARARPEVGRRLERWLSRLLLWASLGLLLVFLGILWVKMGKPSAPQEAEDNMKLLPVDCERKTDEFCQAKQKAALLELLHELYNFLAIQAGNFECGNPENLKSKCIPVMEAQEYIANVTSSSSAKFEAALTWILSSNKDVGIWLKGEDQSELVTTVDKVVCLESAHPRMGVGCRLSRALLTAVTNVLIFFWCLAFLWGLLILLKYRWRKLEEEEQAMYEMVKKIIDVVQDHYVDWEQDMERYPYVGILHVRDSLIPPQSRRRMKRVWDRAVEFLASNESRIQTESHRVAGEDMLVWRWTKPSSFSDSER 5C24BF18B0423952 lap2a_human Lamina-associated polypeptide 2, isoform alpha TMPO LAP2 Thymopoietin isoform alpha Thymopoietin-related peptide isoform alpha protein protein human Homo sapiens Human MPEFLEDPSVLTKDKLKSELVANNVTLPAGEQRKDVYVQLYLQHLTARNRPPLPAGTNSKGPPDFSSDEEREPTPVLGSGAAAAGRSRAAVGRKATKKTDKPRQEDKDDLDVTELTNEDLLDQLVKYGVNPGPIVGTTRKLYEKKLLKLREQGTESRSSTPLPTISSSAENTRQNGSNDSDRYSDNEEGKKKEHKKVKSTRDIVPFSELGTTPSGGGFFQGISFPEISTRPPLGSTELQAAKKVHTSKGDLPREPLVATNLPGRGQLQKLASERNLFISCKSSHDRCLEKSSSSSSQPEHSAMLVSTAASPSLIKETTTGYYKDIVENICGREKSGIQPLCPERSHISDQSPLSSKRKALEESESSQLISPPLAQAIRDYVNSLLVQGGVGSLPGTSNSMPPLDVENIQKRIDQSKFQETEFLSPPRKVPRLSEKSVEERDSGSFVAFQNIPGSELMSSFAKTVVSHSLTTLGLEVAKQSQHDKIDASELSFPFHESILKVIEEEWQQVDRQLPSLACKYPVSSREATQILSVPKVDDEILGFISEATPLGGIQAASTESCNQQLDLALCRAYEAAASALQIATHTAFVAKAMQADISQAAQILSSDPSRTHQALGILSKTYDAASYICEAAFDEVKMAAHTMGNATVGRRYLWLKDCKINLASKNKLASTPFKGGTLFGGEVCKVIKKRGNKH 1B514B0FB61D0D75 p02545-2 Prelamin-A/C Lamin C LMNA LMN1 protein protein human Homo sapiens Human METPSQRRATRSGAQASSTPLSPTRITRLQEKEDLQELNDRLAVYIDRVRSLETENAGLRLRITESEEVVSREVSGIKAAYEAELGDARKTLDSVAKERARLQLELSKVREEFKELKARNTKKEGDLIAAQARLKDLEALLNSKEAALSTALSEKRTLEGELHDLRGQVAKLEAALGEAKKQLQDEMLRRVDAENRLQTMKEELDFQKNIYSEELRETKRRHETRLVEIDNGKQREFESRLADALQELRAQHEDQVEQYKKELEKTYSAKLDNARQSAERNSNLVGAAHEELQQSRIRIDSLSAQLSQLQKQLAAKEAKLRDLEDSLARERDTSRRLLAEKEREMAEMRARMQQQLDEYQELLDIKLALDMEIHAYRKLLEGEEERLRLSPSPTSQRSRGRASSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQNTWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHVSGSRR CFF0FE0F230FCDE2 lmna_human Prelamin-A/C LMNA LMN1 protein protein human Homo sapiens Human METPSQRRATRSGAQASSTPLSPTRITRLQEKEDLQELNDRLAVYIDRVRSLETENAGLRLRITESEEVVSREVSGIKAAYEAELGDARKTLDSVAKERARLQLELSKVREEFKELKARNTKKEGDLIAAQARLKDLEALLNSKEAALSTALSEKRTLEGELHDLRGQVAKLEAALGEAKKQLQDEMLRRVDAENRLQTMKEELDFQKNIYSEELRETKRRHETRLVEIDNGKQREFESRLADALQELRAQHEDQVEQYKKELEKTYSAKLDNARQSAERNSNLVGAAHEELQQSRIRIDSLSAQLSQLQKQLAAKEAKLRDLEDSLARERDTSRRLLAEKEREMAEMRARMQQQLDEYQELLDIKLALDMEIHAYRKLLEGEEERLRLSPSPTSQRSRGRASSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQNTWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHGSHCSSSGDPAEYNLRSRTVLCGTCGQPADKASASGSGAQVGGPISSGSSASSVTVTRSYRSVGGSGGGSFGDNLVTRSYLLGNSSPRTQSPQNCSIM E0855F7699F0318B lemd2-lmna-1 1 3 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 5 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 10 13 direct interaction direct interaction 4F lmna-tmpo-2 1 4 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 5 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 4F lemd2-lmna-2 2 3 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 7