UniProt UniProt http://www.uniprot.org High mobility group box 1 protein interacts with multiple Toll-like receptors. High mobility group box 1 protein interacts with multiple Toll-like receptors. American journal of physiology. Cell physiology 2006 imex curation Park JS., Gamboni-Robertson F., He Q., Svetkauskaite D., Kim JY., Strassheim D., Sohn JW., Yamada S., Maruyama I., Banerjee A., Ishizaka A., Abraham E. edward.abraham@uchsc.edu 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-raw264_7 Mus musculus monocyte macrophage cell line RAW264 raw264_7 Mouse monocyte macrophage cellline RAW264.7 fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Park JS., Gamboni-Robertson F., He Q., Svetkauskaite D., Kim JY., Strassheim D., Sohn JW., Yamada S., Maruyama I., Banerjee A., Ishizaka A., Abraham E. Detected extracellular HMGB1 might derive from exogenous Pig HGMB1 protein or endogenous (mouse) HMGB1. However the experimental conditions used would imply endogenous HMGB1 release has not been stimulated, so it has been assumed only Pig HMGB1 has been detected. FRET:rather than using fluorophore fused proteins, Cy3-labeled (anti-HMGB1) and Alexa Fluor 488-labeled (anti-TLR2/4) labelled antibodies were used. However it is not clear if the primary or secondary antibodies were labelled with fluorophores. Cells have been incubated with pig HMGB1 for 5, 15 and 60 min. edward.abraham@uchsc.edu Accepted 2015-AUG-03 by MGT American journal of physiology. Cell physiology 2006 IMEx imex curation High mobility group box 1 protein interacts with multiple Toll-like receptors. High mobility group box 1 protein interacts with multiple Toll-like receptors. American journal of physiology. Cell physiology 2006 imex curation Park JS., Gamboni-Robertson F., He Q., Svetkauskaite D., Kim JY., Strassheim D., Sohn JW., Yamada S., Maruyama I., Banerjee A., Ishizaka A., Abraham E. edward.abraham@uchsc.edu 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-raw264_7 Mus musculus monocyte macrophage cell line RAW264 raw264_7 Mouse monocyte macrophage cellline RAW264.7 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Park JS., Gamboni-Robertson F., He Q., Svetkauskaite D., Kim JY., Strassheim D., Sohn JW., Yamada S., Maruyama I., Banerjee A., Ishizaka A., Abraham E. Cells have been incubated with pig HMGB1 for 5, 15 and 60 min. edward.abraham@uchsc.edu Accepted 2015-AUG-03 by MGT American journal of physiology. Cell physiology 2006 IMEx imex curation hmgb1_pig High mobility group protein B1 HMGB1 HMG1 High mobility group protein 1 protein protein pig Sus scrofa (Pig) Pig MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKHPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEEEEEDDDDE 8BDCAECF68F62AB5 hmgb1_mouse High mobility group protein B1 Hmgb1 Hmg-1 Hmg1 High mobility group protein 1 protein protein mouse Mus musculus Mouse MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEDDEEDEEDEEEEEEEEDEDEEEDDDDE 8A868DE266D552B5 tlr2_mouse Toll-like receptor 2 Tlr2 protein protein mouse Mus musculus Mouse MLRALWLFWILVAITVLFSKRCSAQESLSCDASGVCDGRSRSFTSIPSGLTAAMKSLDLSFNKITYIGHGDLRACANLQVLILKSSRINTIEGDAFYSLGSLEHLDLSDNHLSSLSSSWFGPLSSLKYLNLMGNPYQTLGVTSLFPNLTNLQTLRIGNVETFSEIRRIDFAGLTSLNELEIKALSLRNYQSQSLKSIRDIHHLTLHLSESAFLLEIFADILSSVRYLELRDTNLARFQFSPLPVDEVSSPMKKLAFRGSVLTDESFNELLKLLRYILELSEVEFDDCTLNGLGDFNPSESDVVSELGKVETVTIRRLHIPQFYLFYDLSTVYSLLEKVKRITVENSKVFLVPCSFSQHLKSLEFLDLSENLMVEEYLKNSACKGAWPSLQTLVLSQNHLRSMQKTGEILLTLKNLTSLDISRNTFHPMPDSCQWPEKMRFLNLSSTGIRVVKTCIPQTLEVLDVSNNNLDSFSLFLPRLQELYISRNKLKTLPDASLFPVLLVMKIRENAVSTFSKDQLGSFPKLETLEAGDNHFVCSCELLSFTMETPALAQILVDWPDSYLCDSPPRLHGHRLQDARPSVLECHQAALVSGVCCALLLLILLVGALCHHFHGLWYLRMMWAWLQAKRKPKKAPCRDVCYDAFVSYSEQDSHWVENLMVQQLENSDPPFKLCLHKRDFVPGKWIIDNIIDSIEKSHKTVFVLSENFVRSEWCKYELDFSHFRLFDENNDAAILVLLEPIERKAIPQRFCKLRKIMNTKTYLEWPLDEGQQEVFWVNLRTAIKS DIP protein Q9QUN7 original sequence version: 131 606D56BF85F320A2 tlr4_mouse Toll-like receptor 4 Tlr4 Lps protein protein mouse Mus musculus Mouse MMPPWLLARTLIMALFFSCLTPGSLNPCIEVVPNITYQCMDQKLSKVPDDIPSSTKNIDLSFNPLKILKSYSFSNFSELQWLDLSRCEIETIEDKAWHGLHHLSNLILTGNPIQSFSPGSFSGLTSLENLVAVETKLASLESFPIGQLITLKKLNVAHNFIHSCKLPAYFSNLTNLVHVDLSYNYIQTITVNDLQFLRENPQVNLSLDMSLNPIDFIQDQAFQGIKLHELTLRGNFNSSNIMKTCLQNLAGLHVHRLILGEFKDERNLEIFEPSIMEGLCDVTIDEFRLTYTNDFSDDIVKFHCLANVSAMSLAGVSIKYLEDVPKHFKWQSLSIIRCQLKQFPTLDLPFLKSLTLTMNKGSISFKKVALPSLSYLDLSRNALSFSGCCSYSDLGTNSLRHLDLSFNGAIIMSANFMGLEELQHLDFQHSTLKRVTEFSAFLSLEKLLYLDISYTNTKIDFDGIFLGLTSLNTLKMAGNSFKDNTLSNVFANTTNLTFLDLSKCQLEQISWGVFDTLHRLQLLNMSHNNLLFLDSSHYNQLYSLSTLDCSFNRIETSKGILQHFPKSLAFFNLTNNSVACICEHQKFLQWVKEQKQFLVNVEQMTCATPVEMNTSLVLDFNNSTCYMYKTIISVSVVSVIVVSTVAFLIYHFYFHLILIAGCKKYSRGESIYDAFVIYSSQNEDWVRNELVKNLEEGVPRFHLCLHYRDFIPGVAIAANIIQEGFHKSRKVIVVVSRHFIQSRWCIFEYEIAQTWQFLSSRSGIIFIVLEKVEKSLLRQQVELYRLLSRNTYLEWEDNPLGRHIFWRRLKNALLDGKASNPEQTAEEEQETATWT DIP protein Q9QUK6 original sequence version: 103 DIP protein Q9QUK6 original sequence version: 118 DIP protein Q9QUK6 original sequence version: 71 9C83B59F9A220C17 hmgb1-tlr2-1 1 3 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept 5 unspecified role unspecified role fluorescence donor fluorescence donor proximity proximity close-contact colocalization neighbourhood interaction 1A Association shown after 5 min of incubation, decreased intensity after 60 min. tlr4-hmgb1-2 1 6 unspecified role unspecified role fluorescence donor fluorescence donor 3 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept proximity proximity close-contact colocalization neighbourhood interaction 1B Association shown after 5 min of incubation, decreased intensity after 60 min. tlr4-hmgb1-3 2 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 3B Detected HMGB1 might derive from added pig HGMB1 protein or endogenous (mouse) HMGB1. However, an association of endogenous proteins is shown in the C60 lane. An increase of association shown after 5 min of incubation, decreased intensity after 60 min. tlr2-hmgb1-1 2 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 3A Detected HMGB1 might derive from added pig HGMB1 protein or endogenous (mouse) HMGB1. However, an association of endogenous proteins is shown in the C60 Lane . An increase of association is shown after 5 min of incubation, decreased intensity after 60 min.