IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Genetic and biochemical interactions among Yar1, Ltv1 and Rps3 define novel links between environmental stress and ribosome biogenesis in Saccharomyces cerevisiae. Genetic and biochemical interactions among Yar1, Ltv1 and Rps3 define novel links between environmental stress and ribosome biogenesis in Saccharomyces cerevisiae. Genetics 2004 imex curation Loar JW., Seiser RM., Sundberg AE., Sagerson HJ., Ilias N., Zobel-Thropp P., Craig EA., Lycan DE. lycan@lclark.edu 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Loar JW., Seiser RM., Sundberg AE., Sagerson HJ., Ilias N., Zobel-Thropp P., Craig EA., Lycan DE. lycan@lclark.edu Accepted 2015-AUG-04 by ORCHARD Genetics 2004 imex curation Genetic and biochemical interactions among Yar1, Ltv1 and Rps3 define novel links between environmental stress and ribosome biogenesis in Saccharomyces cerevisiae. Genetic and biochemical interactions among Yar1, Ltv1 and Rps3 define novel links between environmental stress and ribosome biogenesis in Saccharomyces cerevisiae. Genetics 2004 imex curation Loar JW., Seiser RM., Sundberg AE., Sagerson HJ., Ilias N., Zobel-Thropp P., Craig EA., Lycan DE. lycan@lclark.edu 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast cosedimentation cosedimentation western blot western blot Immuno blot Immuno blot Loar JW., Seiser RM., Sundberg AE., Sagerson HJ., Ilias N., Zobel-Thropp P., Craig EA., Lycan DE. lycan@lclark.edu Accepted 2015-AUG-04 by ORCHARD Genetics 2004 imex curation Genetic and biochemical interactions among Yar1, Ltv1 and Rps3 define novel links between environmental stress and ribosome biogenesis in Saccharomyces cerevisiae. Genetic and biochemical interactions among Yar1, Ltv1 and Rps3 define novel links between environmental stress and ribosome biogenesis in Saccharomyces cerevisiae. Genetics 2004 imex curation Loar JW., Seiser RM., Sundberg AE., Sagerson HJ., Ilias N., Zobel-Thropp P., Craig EA., Lycan DE. lycan@lclark.edu 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Loar JW., Seiser RM., Sundberg AE., Sagerson HJ., Ilias N., Zobel-Thropp P., Craig EA., Lycan DE. lycan@lclark.edu Accepted 2015-AUG-13 by ORCHARD Yeast cDNA Genetics 2004 imex curation ltv1_yeast Protein LTV1 LTV1 YKL143W YKL2 Low-temperature viability protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSKKFSSKNSQRYVVVHRPHDDPSFYDTDASAHVLVPVSNPNKTSPEADLRKKDVSSTKPKGRRAHVGEAALYGINFDDSEYDYTQHLKPIGLDPENSIFIASKGNEQKVEKKNIEDLFIEPKYRRDEIEKDDALPVFQRGMAKPEYLLHQQDTTDEIRGFKPDMNPALREVLEALEDEAYVVNDDVVVEDISKKTQLQGDNYGEEEKEDDIFAQLLGSGEAKDEDEFEDEFDEWDIDNVENFEDENYVKEMAQFDNIENLEDLENIDYQADVRRFQKDNSILEKHNSDDEFSNAGLDSVNPSEEEDVLGELPSIQDKSKTGKKKRKSRQKKGAMSDVSGFSMSSSAIARTETMTVLDDQYDQIINGYENYEEELEEDEEQNYQPFDMSAERSDFESMLDDFLDNYELESGGRKLAKKDKEIERLKEAADEVSKGKLSQRRNRERQEKKKLEKVTNTLSSLKF F432C239B3B0A68A F432C239B3B0A68A sfm1_yeast Protein arginine N-methyltransferase SFM1 SFM1 SPOUT family methyltransferase 1 OR26.11 YOR021C protein protein yeast Saccharomyces cerevisiae Baker's yeast MKYIIEHMEEGFSEWVILEYSQILREVGAENLILSSLPESTTEKDIPQRLLKLGLRWTTKDLKGINEDFKDLELLKDGRVCLLDPRATIDLQPEDATKFDYFVFGGILGDHPPRDRTKELKTAYPNLLISRRLGDKQMTTDTAIRTTQLIIKDRIAFEDIKFIDYPEFRFNKNEATEMPFRYVLDKEGKPILPEGMLDLIKKDSAQSLDDLLM 11A1FB089B39E7EA rs3_yeast 40S ribosomal protein S3 RPS3 SUF14 YNL178W N1653 YS3 RP13 Small ribosomal subunit protein uS3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVALISKKRKLVADGVFYAELNEFFTRELAEEGYSGVEVRVTPTKTEVIIRATRTQDVLGENGRRINELTLLVQKRFKYAPGTIVLYAERVQDRGLSAVAQAESMKFKLLNGLAIRRAAYGVVRYVMESGAKGCEVVVSGKLRAARAKAMKFADGFLIHSGQPVNDFIDTATRHVLMRQGVLGIKVKIMRDPAKSRTGPKALPDAVTIIEPKEEEPILAPSVKDYRPAEETEAQAEPVEA B85C0DFEB011745F B85C0DFEB011745F rs2_yeast 40S ribosomal protein S2 RPS2 SUP38 SUP44 YGL123W G2893 RPS4 S4 YS5 RP12 Omnipotent suppressor protein SUP44 Small ribosomal subunit protein uS5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAPEAQQQKRGGFGGRNRGRPNRRGPRNTEEKGWVPVTKLGRLVKAGKITTIEEIFLHSLPVKEFQIIDTLLPGLQDEVMNIKPVQKQTRAGQRTRFKAVVVVGDSNGHVGLGIKTAKEVAGAIRAGIIIAKLSVIPIRRGYWGTNLGQPHSLATKTTGKCGSVTVRLIPAPRGSGIVASPAVKKLLQLAGVEDVYTQSNGKTRTLENTLKAAFVAIGNTYGFLTPNLWAEQPLPVSPLDIYSDEASAQKKRF 5EFDA4C7DE7BDFFB yar1_yeast Ankyrin repeat-containing protein YAR1 YPL239W YAR1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGLHSEPLDQEDQDTIILDARAGDLDSLKDIFTTLVSPELLSTCKESESDSTALHMAAANGHIETVRYILETVSRANSAEDLKAFVNEVNKTGNTALHWASLNGKLDVVKLLCDEYEADPFIRNKFGHDAIFEAENSGKEEVETYFLKKYDVEPEDDEEDTQTEGKNSVQITKGTEIEQVTKEATEALREETEKLNINKD 8089CFC062E65395 rl3_yeast 60S ribosomal protein L3 MAK8 RPL3 TCM1 YOR063W YOR29-14 YL1 RP1 Maintenance of killer protein 8 Trichodermin resistance protein Large ribosomal subunit protein uL3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSHRKYEAPRHGHLGFLPRKRAASIRARVKAFPKDDRSKPVALTSFLGYKAGMTTIVRDLDRPGSKFHKREVVEAVTVVDTPPVVVVGVVGYVETPRGLRSLTTVWAEHLSDEVKRRFYKNWYKSKKKAFTKYSAKYAQDGAGIERELARIKKYASVVRVLVHTQIRKTPLAQKKAHLAEIQLNGGSISEKVDWAREHFEKTVAVDSVFEQNEMIDAIAVTKGHGFEGVTHRWGTKKLPRKTHRGLRKVACIGAWHPAHVMWSVARAGQRGYHSRTSINHKIYRVGKGDDEANGATSFDRTKKTITPMGGFVHYGEIKNDFIMVKGCIPGNRKRIVTLRKSLYTNTSRKALEEVSLKWIDTASKFGKGRFQTPAEKHAFMGTLKKDL 986769A218E9698A yar1_rps3-1 3 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Fig2C yar1_ltv1-1 3 4 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait P46683:p.Lys91Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K A Wed May 02 14:30:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys91ala' Tue Jun 30 17:26:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys91Ala' P46683:p.Lys125Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K A This mutanion has no effect when alone but increases the effect of K91 mutation Wed May 02 18:48:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys125ala' Tue Jun 30 17:22:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys125Ala' physical association physical association Fig2B yar_rs3-1 1 6 unspecified role unspecified role prey prey label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 8 unspecified role unspecified role bait bait GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig1A ltv1_l3-1 2 7 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component HA tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role neutral component neutral component myc myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Fig7 Only a small fraction of Yar1 is associated to S2, while Ltv1 is almost all associated with S2 but it colocalize with the 40S subunit, not with polysomes yar1-sfm1-1 3 5 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association