IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ An Lsm2-Lsm7 complex in Saccharomyces cerevisiae associates with the small nucleolar RNA snR5. An Lsm2-Lsm7 complex in Saccharomyces cerevisiae associates with the small nucleolar RNA snR5. Molecular biology of the cell 2004 imex curation Fernandez CF., Pannone BK., Chen X., Fuchs G., Wolin SL. sandra.wolin@yale.edu 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Fernandez CF., Pannone BK., Chen X., Fuchs G., Wolin SL. sandra.wolin@yale.edu Accepted 2015-AUG-18 by ORCHARD Molecular biology of the cell 2004 imex curation An Lsm2-Lsm7 complex in Saccharomyces cerevisiae associates with the small nucleolar RNA snR5. An Lsm2-Lsm7 complex in Saccharomyces cerevisiae associates with the small nucleolar RNA snR5. Molecular biology of the cell 2004 imex curation Fernandez CF., Pannone BK., Chen X., Fuchs G., Wolin SL. sandra.wolin@yale.edu 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip northern blot northern blot northen Fernandez CF., Pannone BK., Chen X., Fuchs G., Wolin SL. sandra.wolin@yale.edu Accepted 2015-AUG-18 by ORCHARD Molecular biology of the cell 2004 imex curation An Lsm2-Lsm7 complex in Saccharomyces cerevisiae associates with the small nucleolar RNA snR5. An Lsm2-Lsm7 complex in Saccharomyces cerevisiae associates with the small nucleolar RNA snR5. Molecular biology of the cell 2004 imex curation Fernandez CF., Pannone BK., Chen X., Fuchs G., Wolin SL. sandra.wolin@yale.edu 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast cosedimentation cosedimentation northern blot northern blot northen Fernandez CF., Pannone BK., Chen X., Fuchs G., Wolin SL. sandra.wolin@yale.edu Accepted 2015-AUG-18 by ORCHARD Molecular biology of the cell 2004 imex curation An Lsm2-Lsm7 complex in Saccharomyces cerevisiae associates with the small nucleolar RNA snR5. An Lsm2-Lsm7 complex in Saccharomyces cerevisiae associates with the small nucleolar RNA snR5. Molecular biology of the cell 2004 imex curation Fernandez CF., Pannone BK., Chen X., Fuchs G., Wolin SL. sandra.wolin@yale.edu 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip northern blot northern blot northen Fernandez CF., Pannone BK., Chen X., Fuchs G., Wolin SL. sandra.wolin@yale.edu Accepted 2015-AUG-18 by ORCHARD Molecular biology of the cell 2004 imex curation smd1_yeast Small nuclear ribonucleoprotein Sm D1 YGR074W SMD1 snRNP core protein D1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKLVNFLKKLRNEQVTIELKNGTTVWGTLQSVSPQMNAILTDVKLTLPQPRLNKLNSNGIAMASLYLTGGQQPTASDNIASLQYINIRGNTIRQIILPDSLNLDSLLVDQKQLNSLRRSGQIANDPSKKRRRDFGAPANKRPRRGL 9929F21EB9218447 9929F21EB9218447 ruxg_yeast Small nuclear ribonucleoprotein G SNP2 YFL017Bw YFL017W-A SMX2 Sm protein G protein protein yeast Saccharomyces cerevisiae Baker's yeast MVSTPELKKYMDKKILLNINGSRKVAGILRGYDIFLNVVLDDAMEINGEDPANNHQLGLQTVIRGNSIISLEALDAI 5C6208B8C5A9AB33 5C6208B8C5A9AB33 ruxf_yeast Small nuclear ribonucleoprotein F SMX3 YPR182W P9705.4 Sm protein F protein protein yeast Saccharomyces cerevisiae Baker's yeast MSESSDISAMQPVNPKPFLKGLVNHRVGVKLKFNSTEYRGTLVSTDNYFNLQLNEAEEFVAGVSHGTLGEIFIRCNNVLYIRELPN 1640D3237597B2D8 1640D3237597B2D8 smd2_yeast Small nuclear ribonucleoprotein Sm D2 SMD2 YLR275W L9328.5 snRNP core protein D2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSQIIDRPKHELSRAELEELEEFEFKHGPMSLINDAMVTRTPVIISLRNNHKIIARVKAFDRHCNMVLENVKELWTEKKGKNVINRERFISKLFLRGDSVIVVLKTPVE 7287070B10E090C0 7287070B10E090C0 ruxe_yeast Small nuclear ribonucleoprotein E SME1 YOR159C Sm protein E protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNKVKTKAMVPPINCIFNFLQQQTPVTIWLFEQIGIRIKGKIVGFDEFMNVVIDEAVEIPVNSADGKEDVEKGTPLGKILLKGDNITLITSAD B76D72DA8456F3B5 B76D72DA8456F3B5 lsm2_yeast U6 snRNA-associated Sm-like protein LSm2 LSM2 Small nuclear ribonucleoprotein D homolog SNP3 SMX5 SNP3 YBL0425 YBL026W protein protein yeast Saccharomyces cerevisiae Baker's yeast MLFFSFFKTLVDQEVVVELKNDIEIKGTLQSVDQFLNLKLDNISCTDEKKYPHLGSVRNIFIRGSTVRYVYLNKNMVDTNLLQDATRREVMTERK C23856268CEE92E2 C23856268CEE92E2 snru3a yeast small nuclear RNA U3a Yeast snrna small nuclear rna snRNA snrna yeast Saccharomyces cerevisiae Baker's yeast GUCGACGUACUUCAGUAUGUAAUAUACCCCAAACAUUUUACCCACAAAAAACCAGGAUUUGAAAACUAUAGCAUCUAAAAGUCUUAGGUACUAGAGUUUUCAUUUCGGAGCAGGCUUUUUGAAAAAUUUAAUUCAACCAUUGCAGCAGCUUUUGACUAACACAUUCUACAGUAGGAUCAUUUCUAUAGGAAUCGUCACUCUUUGACUCUUCAAAAGAGCCACUGAAUCCAACUUGGUUGAUGAGUCCCAUAACCUUUGUACCCCAGAGUGAGAAACCGAAAUUGAAUCUAAAUUAGCUUGGUCCGCAAUCCUUAGCGGUUCGGCCAUCUAUAAUUUUGAAUAAAAAUUUUGCUUUGCCGUUGCAUUUGUAGUUUUUUCCUUUGGAAGUAAUUACAAUAUUUUAUGGCGCGAUGAUCUUGACCCAUCCUAUGUACUUCUUUUUUGAAGGGAUAGGGCUCUAUGGGUGGGUACAAAUGGCAGUCUGACAAGU lsm3_yeast U6 snRNA-associated Sm-like protein LSm3 LSM3 SMX4 USS2 YLR438C-A SmX4 protein protein protein yeast Saccharomyces cerevisiae Baker's yeast METPLDLLKLNLDERVYIKLRGARTLVGTLQAFDSHCNIVLSDAVETIYQLNNEELSESERRCEMVFIRGDTVTLISTPSEDDDGAVEI 787084EF6A5945B7 787084EF6A5945B7 lsm4_yeast U6 snRNA-associated Sm-like protein LSm4 LSM4 SDB23 USS1 YER112W protein protein yeast Saccharomyces cerevisiae Baker's yeast MLPLYLLTNAKGQQMQIELKNGEIIQGILTNVDNWMNLTLSNVTEYSEESAINSEDNAESSKAVKLNEIYIRGTFIKFIKLQDNIIDKVKQQINSNNNSNSNGPGHKRYYNNRDSNNNRGNYNRRNNNNGNSNRRPYSQNRQYNNSNSSNINNSINSINSNNQNMNNGLGGSVQHHFNSSSPQKVEF 69CAC1F01D5BFA98 69CAC1F01D5BFA98 snr7s_u5_yeast small nuclear RNA U5 Yeast snrna small nuclear rna snRNA snrna yeast Saccharomyces cerevisiae Baker's yeast AAGCAGCUUUACAGAUCAAUGGCGGAGGGAGGUCAACAUCAAGAACUGUGGGCCUUUUAUUGCCUAUAGAACUUAUAACGAACAUGGUUCUUGCCUUUUACCAGAACCAUCCGGGUGUUGUCUCCAUAGAAACAGGUAAAGCUGUCCGUUACUGUGGGCUUGCCAUAUUUUUUGGAACU nhp2_yeast H/ACA ribonucleoprotein complex subunit NHP2 NHP2 YDL208W D1045 H/ACA snoRNP protein NHP2 High mobility group-like nuclear protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGKDNKEHKESKESKTVDNYEARMPAVLPFAKPLASKKLNKKVLKTVKKASKAKNVKRGVKEVVKALRKGEKGLVVIAGDISPADVISHIPVLCEDHSVPYIFIPSKQDLGAAGATKRPTSVVFIVPGSNKKKDGKNKEEEYKESFNEVVKEVQAL 008188132B494E5D 008188132B494E5D smd3_yeast Small nuclear ribonucleoprotein Sm D3 SMD3 YLR147C L9634.6 snRNP core protein D3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTMNGIPVKLLNEAQGHIVSLELTTGATYRGKLVESEDSMNVQLRDVIATEPQGAVTHMDQIFVRGSQIKFIVVPDLLKNAPLFKKNSSRPMPPIRGPKRR BB02A56C5AD70CF2 BB02A56C5AD70CF2 lsm6_yeast U6 snRNA-associated Sm-like protein LSm6 LSM6 D9481.18 YDR378C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGKASTEGSVTTEFLSDIIGKTVNVKLASGLLYSGRLESIDGFMNVALSSATEHYESNNNKLLNKFNSDVFLRGTQVMYISEQKI 74FE40A4509CEF33 74FE40A4509CEF33 lsm5_yeast U6 snRNA-associated Sm-like protein LSm5 LSM5 YER146W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLPEILPLEVIDKTINQKVLIVLQSNREFEGTLVGFDDFVNVILEDAVEWLIDPEDESRNEKVMQHHGRMLLSGNNIAILVPGGKKTPTEAL 84E8A1E8E2F078FE 84E8A1E8E2F078FE lsm8_yeast U6 snRNA-associated Sm-like protein LSm8 LSM8 J1464 YJR83.16 YJR022W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSATLKDYLNKRVVIIKVDGECLIASLNGFDKNTNLFITNVFNRISKEFICKAQLLRGSEIALVGLIDAENDDSLAPIDEKKVPMLKDTKNKIENEHVIWEKVYESKTK B6E37F585B6292EA B6E37F585B6292EA lsm7_yeast U6 snRNA-associated Sm-like protein LSm7 LSM7 N1202 N1780 YNL147W protein protein yeast Saccharomyces cerevisiae Baker's yeast MHQQHSKSENKPQQQRKKFEGPKREAILDLAKYKDSKIRVKLMGGKLVIGVLKGYDQLMNLVLDDTVEYMSNPDDENNTELISKNARKLGLTVIRGTILVSLSSAEGSDVLYMQK EA1D019FC5D1B5C5 EA1D019FC5D1B5C5 rsmb_yeast Small nuclear ribonucleoprotein-associated protein B SMB1 Sm protein B YER029C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSKIQVAHSSRLANLIDYKLRVLTQDGRVYIGQLMAFDKHMNLVLNECIEERVPKTQLDKLRPRKDSKDGTTLNIKVEKRVLGLTILRGEQILSTVVEDKPLLSKKERLVRDKKEKKQAQKQTKLRKEKEKKPGKIAKPNTANAKHTSSNSREIAQPSSSRYNGGNDNIGANRSRFNNEAPPQTRKFQPPPGFKRK 0A1078D5AA241430 0A1078D5AA241430 snr14_u4_yeast SNR14 snrna small nuclear rna snRNA snrna yeast Saccharomyces cerevisiae Baker's yeast AUCCUUAUGCACGGGAAAUACGCAUAUCAGUGAGGAUUCGUCCGAGAUUGUGUUUUUGCUGGUUGAAAUUUAAUUAUAAACCAGACCGUCUCCUCAUGGUCAAUUCGGUGUUCGCUUUUGAAUACUUCAAGACUAUGUAGGGAAUUUUUGGAAUACCUUU snr5 yeast small nucleolar RNA 5 Yeast snorna small nucleolar rna snoRNA snorna yeast Saccharomyces cerevisiae Baker's yeast AUCAUUCAAUAAACUGAUCUUCCGGAUUACCAUGCUUAAGACAUCACGCCUCCAUAUGUCUAUAUAAAGCGCAAAUGGCUGGAAGUAGACCAAUUUUUUUUGUUCCUAGCUUUUCAUUAUUGAAAUCUAAUCCAGUUUUAAUGGUUUUUCUUAAUUAAGAAAACAAAUUAUCAUUGGUUCGCUCUAGGUGUACAUAUCUUCACU snr19_u1_yeast SNR19 snrna small nuclear rna snRNA snrna yeast Saccharomyces cerevisiae Baker's yeast AUACUUACCUUAAGAUAUCAGAGGAGAUCAAGAAGUCCUACUGAUCAAACAUGCGCUUCCAAUAGUAGAAGGACGUUAAGCAUUUAUCAUUGAACUAUAAUUGUUCAUUGAAGUCAUUGAUGCAAACUCCUUGGUCACACACACAUACGGCGCGGAAGGCGUGUUUGCUGACGUUUCCAUUCCCUUGUUUCAAUCAUUGGUUAAUCCCUUGAUUCCUUUGGGGAUUUUUGGGUUAAACUGAUUUUUGGGGCCCUUUGUUUCUUCUGCCUGGAGAAGUUUGACACCAAAUUCAAAUUGGUGUUAGGGGAGCUGGGGCCUUUCAAAAGAGAGCUUUGUAGAGGCAUUCUUUUUGACUACUUUUCUCUAGCGUGCCAUUUUAGUUUUUGACGGCAGAUUCGAAUGAACUUAAGUUUAUGAUGAAGGUAUGGCUGUUGAGAUUAUUUGGUCGGGAUUGUAGUUUGAAGAUGUGCUCUUUUGAGCAGUCUCAACUUUGCUCGUUCCCGUUAUGGGAAAAAUUUUGGAAGGUCUUGGUAGGAACGGGUGGAUCUUAUAAUUUUUGAUUUAUUUU gar1_yeast H/ACA ribonucleoprotein complex subunit GAR1 YHR089C GAR1 snoRNP protein GAR1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFRGGNRGGRGGFRGGFRGGRTGSARSFQQGPPDTVLEMGAFLHPCEGDIVCRSINTKIPYFNAPIYLENKTQVGKVDEILGPLNEVFFTIKCGDGVQATSFKEGDKFYIAADKLLPIERFLPKPKVVGPPKPKNKKKRSGAPGGRGGASMGRGGSRGGFRGGRGGSSFRGGRGGSSFRGGSRGGSFRGGSRGGSRGGFRGGRR C197FE85644DD0F8 C197FE85644DD0F8 snru6_yeast small nuclear RNA U6 Yeast snrna small nuclear rna snRNA snrna yeast Saccharomyces cerevisiae Baker's yeast GUUCGCGAAGUAACCCUUCGUGGACAUUUGGUCAAUUUGAAACAAUACAGAGAUGAUCAGCAGUUCCCCUGCAUAAGGAUGAACCGUUUUACAAAGAGAUUUAUUUCGUUUU snr10_yeast_rna small nucleolar RNA 10 Yeast snorna small nucleolar rna snoRNA snorna yeast Saccharomyces cerevisiae Baker's yeast AACGCAAAUUUAACAGCCAUUCGUAACACGUACAGUAUCUCGUCGAGGUUGAACCCCUCCGGGGCGUUCUUGACCCAUGAAGAAUGUGGAUUGGUGUUGCAAUAUAUUUAUUAUUGGCUUGGACUGGAGAACAAAUUUAUCGAUCUUGGGUGCAACAGUCUUUCUGUCGUCUGUUUUUUAGCAGAUCUAAGGGUUUACCUUCGUGUGCCCGGAUGAGGACCGUUGCAAGGAUUGAUAAUACAACU lsm3_rna-1 4 23 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence northern blot northern blot northen unspecified role unspecified role prey prey label 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain 14 unspecified role unspecified role prey prey label 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain 26 unspecified role unspecified role prey prey label 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 22 unspecified role unspecified role prey prey label 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain association association Fig1 lsm5-snru4-1 4 18 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 23 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence northern blot northern blot northen unspecified role unspecified role prey prey label 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain 14 unspecified role unspecified role prey prey label 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain 26 unspecified role unspecified role prey prey label 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain 22 unspecified role unspecified role prey prey label 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain association association Fig1 lsm8-snru4-1 4 19 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 14 unspecified role unspecified role prey prey label 33p radiolabel 33p radiolabel P33 33P certain certain sequence position certain certain certain sequence position certain 26 unspecified role unspecified role prey prey label 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain 22 unspecified role unspecified role prey prey label 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain association association Fig1 lsm2_snr5-1 4 26 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Fig2A lsm3_snr5-1 4 26 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Fig2A lsm4_snr5-1 4 26 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Fig2A lsm5_snr5-1 4 26 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Fig2A,B Fig5A lsm6_snr5-1 4 26 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 17 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Fig2A lsm7_snr5-1 4 20 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 23 unspecified role unspecified role prey prey association association Fig2A lsm8_u6-1 4 19 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 26 unspecified role unspecified role prey prey association association Fig2A,B smb1-1 4 26 unspecified role unspecified role prey prey 21 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 24 unspecified role unspecified role prey prey association association Fig2A smd1_u1-1 2 26 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait 24 unspecified role unspecified role prey prey association association Fig2A smd2_u1-1 4 26 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 24 unspecified role unspecified role prey prey association association text smd3_u1-1 4 26 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 24 unspecified role unspecified role prey prey association association sme1-1 4 26 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 24 unspecified role unspecified role prey prey association association smf_u1-1 4 26 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 24 unspecified role unspecified role prey prey association association smg_u1-1 4 26 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 24 unspecified role unspecified role prey prey association association text lms2_srn5-1 1 23 unspecified role unspecified role prey prey label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 26 unspecified role unspecified role prey prey Label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 10 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Fig3, Fig4 lsm5-snr5-1 1 18 unspecified role unspecified role bait bait Tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 23 unspecified role unspecified role prey prey label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain UGUACAUAU CCCCCCCCC in vitro In vitro 26 unspecified role unspecified role prey prey Label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro association association Fig3, Fig4 lsm6-snr5-1 1 17 unspecified role unspecified role bait bait tag tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 23 unspecified role unspecified role prey prey label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 26 unspecified role unspecified role prey prey Label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro association association Fig3, Fig4 lsm8_snu6-1 1 26 unspecified role unspecified role prey prey label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 19 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig3 nhp2-5 2 15 unspecified role unspecified role bait bait 27 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey association association Fig5A gar_snr5-1 3 11 unspecified role unspecified role neutral component neutral component 26 unspecified role unspecified role neutral component neutral component 23 unspecified role unspecified role neutral component neutral component 25 anti tag western anti tag western blot anti tag western northern blot northern blot northen unspecified role unspecified role neutral component neutral component tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 22 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig5B lsm5_snr5-2 3 11 unspecified role unspecified role neutral component neutral component 26 unspecified role unspecified role neutral component neutral component 23 unspecified role unspecified role neutral component neutral component 18 anti tag western anti tag western blot anti tag western northern blot northern blot northen unspecified role unspecified role neutral component neutral component tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 24 unspecified role unspecified role neutral component neutral component 22 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig5B