IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The epithelial sodium channel (ENaC) is intracellularly located as a tetramer. The epithelial sodium channel (ENaC) is intracellularly located as a tetramer. Pflugers Archiv : European journal of physiology 2002 imex curation Dijkink L., Hartog A., van Os CH., Bindels RJ. r.bindels@ncmls.kun.nl 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation xenla-oocyte Xenopus laevis oocyte Oocyte anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Dijkink L., Hartog A., van Os CH., Bindels RJ. flag tag positions from PMID:8986818 r.bindels@ncmls.kun.nl Accepted 2016-FEB-04 AT 13:29 GMT AT 13:29 GMT by ORCHARD Pflugers Archiv : European journal of physiology 2002 Only protein-protein interactions imex curation imex curation The epithelial sodium channel (ENaC) is intracellularly located as a tetramer. The epithelial sodium channel (ENaC) is intracellularly located as a tetramer. Pflugers Archiv : European journal of physiology 2002 imex curation Dijkink L., Hartog A., van Os CH., Bindels RJ. r.bindels@ncmls.kun.nl 22-Feb-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation xenla-oocyte Xenopus laevis oocyte Oocyte molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Dijkink L., Hartog A., van Os CH., Bindels RJ. r.bindels@ncmls.kun.nl Accepted 2016-FEB-04 AT 13:29 GMT AT 13:29 GMT by ORCHARD Pflugers Archiv : European journal of physiology 2002 Only protein-protein interactions imex curation imex curation scnna_rat Amiloride-sensitive sodium channel subunit alpha Scnn1a Renac Alpha-NaCH Epithelial Na(+) channel subunit alpha Nonvoltage-gated sodium channel 1 subunit alpha SCNEA protein protein rat Rattus norvegicus (Rat) Rat MLDHTRAPELNIDLDLHASNSPKGSMKGNQFKEQDPCPPQPMQGLGKGDKREEQGLGPEPSAPRQPTEEEEALIEFHRSYRELFQFFCNNTTIHGAIRLVCSKHNRMKTAFWAVLWLCTFGMMYWQFALLFEEYLSYPVSLNINLNSDKLVFPAVTVCTLNPYRYTEIKEELEELDRITEQTLFDLYKYNSSYTRQAGARRRSSRDLLGAFPHPLQRLRTPPPPYSGRTARSGSSSVRDNNPQVDRKDWKIGFQLCNQNKSDCFYQTYSSGVDAVREWYRFHYINILSRLSDTSPALEEEALGNFIFTCRFNQAPCNQANYSKFHHPMYGNCYTFNDKNNSNLWMSSMPGVNNGLSLTLRTEQNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNLRPGVETSISMRKEALDSLGGNYGDCTENGSDVPVKNLYPSKYTQQVCIHSCFQENMIKKCGCAYIFYPKPKGVEFCDYRKQSSWGYCYYKLQGAFSLDSLGCFSKCRKPCSVINYKLSAGYSRWPSVKSQDWIFEMLSLQNNYTINNKRNGVAKLNIFFKELNYKTNSESPSVTMVSLLSNLGSQWSLWFGSSVLSVVEMAELIFDLLVITLLMLLRRFRSRYWSPGRGARGAREVASTPASSFPSRFCPHPTSPPPSLPQQGMTPPLALTAPPPAYATLGPSAPPLDSAAPDCSACALAAL DIP protein P37089 original sequence version: 121 B0CF7C15C3CE9763 scnng_rat Amiloride-sensitive sodium channel subunit gamma Scnn1g Epithelial Na(+) channel subunit gamma Gamma-NaCH Nonvoltage-gated sodium channel 1 subunit gamma SCNEG protein protein rat Rattus norvegicus (Rat) Rat MAPGEKIKAKIKKNLPVRGPQAPTIKDLMHWYCMNTNTHGCRRIVVSRGRLRRLLWIAFTLTAVALIIWQCALLVFSFYTVSVSIKVHFQKLDFPAVTICNINPYKYSAVSDLLTDLDSETKQALLSLYGVKESRKRREAGSMPSTLEGTPPRFFKLIPLLVFNENEKGKARDFFTGRKRKISGKIIHKASNVMHVHESKKLVGFQLCSNDTSDCATYTFSSGINAIQEWYKLHYMNIMAQVPLEKKINMSYSAEELLVTCFFDGMSCDARNFTLFHHPMYGNCYTFNNKENATILSTSMGGSEYGLQVILYINEDEYNPFLVSSTGAKVLIHQQNEYPFIEDVGMEIETAMSTSIGMHLTESFKLSEPYSQCTEDGSDVPVTNIYNAAYSLQICLYSCFQTKMVEKCGCAQYSQPLPPAANYCNYQQHPNWMYCYYQLYQAFVREELGCQSVCKQSCSFKEWTLTTSLAQWPSEASEKWLLNVLTWDQSQQINKKLNKTDLAKLLIFYKDLNQRSIMESPANSIEMLLSNFGGQLGLWMSCSVVCVIEIIEVFFIDFFSIIARRQWHKAKDWWARRQTPPSTETPSSRQGQDNPALDTDDDLPTFTSAMRLPPAPGSTVPGTPPPRYNTLRLDRAFSSQLTDTQLTNEL DIP protein P37091 original sequence version: 117 701F9B28B3250D8F scnnb_rat Amiloride-sensitive sodium channel subunit beta Scnn1b Beta-NaCH Epithelial Na(+) channel subunit beta Nonvoltage-gated sodium channel 1 subunit beta SCNEB protein protein rat Rattus norvegicus (Rat) Rat MPVKKYLLKCLHRLQKGPGYTYKELLVWYCNNTNTHGPKRIICEGPKKKAMWFLLTLLFACLVCWQWGVFIQTYLSWEVSVSLSMGFKTMNFPAVTVCNSSPFQYSKVKHLLKDLYKLMEAVLDKILAPKSSHTNTTSTLNFTIWNHTPLVLIDERNPDHPVVLNLFGDSHNSSNPAPGSTCNAQGCKVAMRLCSANGTVCTFRNFTSATQAVTEWYILQATNIFSQVLPQDLVGMGYAPDRIILACLFGTEPCSHRNFTPIFYPDYGNCYIFNWGMTEKALPSANPGTEFGLKLILDIGQEDYVPFLASTAGARLMLHEQRTYPFIREEGIYAMAGTETSIGVLLDKLQGKGEPYSPCTMNGSDVAIQNLYSDYNTTYSIQACLHSCFQDHMIHNCSCGHYLYPLPAGEKYCNNRDFPDWAYCYLSLQMSVVQRETCLSMCKESCNDTQYKMTISMADWPSEASEDWILHVLSQERDQSSNITLSRKGIVKLNIYFQEFNYRTIEESPANNIVWLLSNLGGQFGFWMGGSVLCLIEFGEIIIDFIWITVIKLVASCKGLRRRRPQAPYTGPPPTVAELVEAHTNFGFQPDTTSCRPNAEVYPDQQTLPIPGTPPPNYDSLRLQPLDTMESDSEVEAI 79176A282D5079F1 scnn1a-scnn1b-1 1 5 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged certain certain sequence position certain certain certain sequence position certain TRQAGARR DYKDDDDK physical association physical association Fig. 6 scnn1g-scnn1b-1 1 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged certain certain sequence position certain certain certain sequence position certain SMPSTLEG DYKDDDDK physical association physical association Fig. 6 scnn1a-scnn1b-2 2 4 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig.s 1 & 2