IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Role of Cue1p in ubiquitination and degradation at the ER surface. Role of Cue1p in ubiquitination and degradation at the ER surface. Science (0036-8075) 1997 imex curation Biederer T., Volkwein C., Sommer T. tsommer@mdc-berlin.de 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Biederer T., Volkwein C., Sommer T. Thioester-bonded proteins were eluted from the column with dithiothreitol tsommer@mdc-berlin.de Accepted 2014-FEB-19 by PPORRAS Science (0036-8075) 1997 imex curation Role of Cue1p in ubiquitination and degradation at the ER surface. Role of Cue1p in ubiquitination and degradation at the ER surface. Science (0036-8075) 1997 imex curation Biederer T., Volkwein C., Sommer T. tsommer@mdc-berlin.de 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom edman degradation edman degradation Biederer T., Volkwein C., Sommer T. Thioester-bonded proteins were eluted from both columns with dithiothreitol. Data relating to GST linked ubuiquitin could not be curated as the source of this construct could not be traced. tsommer@mdc-berlin.de Accepted 2014-FEB-19 by PPORRAS Science (0036-8075) 1997 imex curation ubc7_yeast Ubiquitin-conjugating enzyme E2 7 UBC7 QRI8 YMR022W YM9711.12 Ubiquitin-protein ligase Ubiquitin carrier protein Ubiquitin-conjugating enzyme E2-18 kDa E2 ubiquitin-conjugating enzyme 7 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSKTAQKRLLKELQQLIKDSPPGIVAGPKSENNIFIWDCLIQGPPDTPYADGVFNAKLEFPKDYPLSPPKLTFTPSILHPNIYPNGEVCISILHSPGDDPNMYELAEERWSPVQSVEKILLSVMSMLSEPNIESGANIDACILWRDNRPEFERQVKLSILKSLGF D3D297847DBB462D D3D297847DBB462D cue1_yeast Coupling of ubiquitin conjugation to ER degradation protein 1 KIS4 YM8156.06 YMR264W CUE1 Kinetochore-defect suppressor 4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MEDSRLLITLILVFGVIFLKKFFQSNQHPSAQRLSATGVNAHGRPQGSTQNALRRTGRVNGGHPVTTQMVETVQNLAPNLHPEQIRYSLENTGSVEETVERYLRGDEFSFPPGFEPSRAPMGANAAVDNNAAGGGEFNDPRKKNMICAENLLDKFHVDLNEDMSNLSFKDLDIEERKRLLVWQARKNLETKLQSDKDLQSLLT 3F795BD78DAC3D5B ubiq_bovin Ubiquitin RPS27A UBA80 UBCEP1 UBA52 UBCEP2 UBB UBC protein protein bovin Bos taurus (Bovine) Bovine MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG C42A35397FFD9B52 rps27a-ubi1 2 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey yeast Saccharomyces cerevisiae Baker's yeast physical association physical association Fig 1A cue1-ubc7 1 4 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association