IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Cross-phosphorylation of bacterial serine/threonine and tyrosine protein kinases on key regulatory residues. Cross-phosphorylation of bacterial serine/threonine and tyrosine protein kinases on key regulatory residues. Front Microbiol 2014 imex curation Shi L., Pigeonneau N., Ravikumar V., Dobrinic P., Macek B., Franjevic D., Noirot-Gros M.F., Mijakovic I. ivan.mijakovic@chalmers.se, marie-francoise.gros@jouy.inra.fr 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight by staining molecular weight estimation by staining weight by staining Shi L., Pigeonneau N., Ravikumar V., Dobrinic P., Macek B., Franjevic D., Noirot-Gros M.F., Mijakovic I. ivan.mijakovic@chalmers.se, marie-francoise.gros@jouy.inra.fr Accepted 2014-OCT-31 by MGT Front Microbiol 2014 Only protein-protein interactions imex curation Cross-phosphorylation of bacterial serine/threonine and tyrosine protein kinases on key regulatory residues. Cross-phosphorylation of bacterial serine/threonine and tyrosine protein kinases on key regulatory residues. Front Microbiol 2014 imex curation Shi L., Pigeonneau N., Ravikumar V., Dobrinic P., Macek B., Franjevic D., Noirot-Gros M.F., Mijakovic I. ivan.mijakovic@chalmers.se, marie-francoise.gros@jouy.inra.fr 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay autoradiography autoradiography Shi L., Pigeonneau N., Ravikumar V., Dobrinic P., Macek B., Franjevic D., Noirot-Gros M.F., Mijakovic I. ivan.mijakovic@chalmers.se, marie-francoise.gros@jouy.inra.fr Accepted 2014-OCT-31 by MGT Front Microbiol 2014 Only protein-protein interactions imex curation Cross-phosphorylation of bacterial serine/threonine and tyrosine protein kinases on key regulatory residues. Cross-phosphorylation of bacterial serine/threonine and tyrosine protein kinases on key regulatory residues. Front Microbiol 2014 imex curation Shi L., Pigeonneau N., Ravikumar V., Dobrinic P., Macek B., Franjevic D., Noirot-Gros M.F., Mijakovic I. ivan.mijakovic@chalmers.se, marie-francoise.gros@jouy.inra.fr 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Shi L., Pigeonneau N., Ravikumar V., Dobrinic P., Macek B., Franjevic D., Noirot-Gros M.F., Mijakovic I. ivan.mijakovic@chalmers.se, marie-francoise.gros@jouy.inra.fr Accepted 2014-OCT-31 by MGT Front Microbiol 2014 Only protein-protein interactions imex curation Cross-phosphorylation of bacterial serine/threonine and tyrosine protein kinases on key regulatory residues. Cross-phosphorylation of bacterial serine/threonine and tyrosine protein kinases on key regulatory residues. Front Microbiol 2014 imex curation Shi L., Pigeonneau N., Ravikumar V., Dobrinic P., Macek B., Franjevic D., Noirot-Gros M.F., Mijakovic I. ivan.mijakovic@chalmers.se, marie-francoise.gros@jouy.inra.fr 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay sequence tag sequence tag identification sequence tag Shi L., Pigeonneau N., Ravikumar V., Dobrinic P., Macek B., Franjevic D., Noirot-Gros M.F., Mijakovic I. ivan.mijakovic@chalmers.se, marie-francoise.gros@jouy.inra.fr Accepted 2014-OCT-31 by MGT Front Microbiol 2014 Only protein-protein interactions imex curation yvel_bacsu Putative tyrosine-protein kinase YveL yveL BSU34360 protein protein bacsu Bacillus subtilis MIFRKKKARRGLAQISVLHNKSVVAEQYRTIRTNIEFSSVQTNLRSILVTSSVPGEGKSFSAANLAAVFAQQQEKKVLLVDADLRKPTINQTFQVDNVTGLTNVLVGNASLSETVQKTPIDNLYVLTSGPTPPNPAELLSSKAMGDLISEIYEQFSLVIFDSPPLLAVADAQILANQTDGSVLVVLSGKTKTDTVLKAKDALEQSNAKLLGALLNKKKMKKSEHYSY 81C8B9D75278FFA9 sp2ab_bacsu Anti-sigma F factor spoIIAB Stage II sporulation protein AB BSU23460 protein protein bacsu Bacillus subtilis MKNEMHLEFSALSQNESFARVTVASFIAQLDPTMDELTEIKTVVSEAVTNAIIHGYEENCEGKVYISVTLEDHVVYMTIRDEGLGITDLEEARQPLFTTKPELERSGMGFTIMENFMDDVSIDSSPEMGTTIRLTKHLSKSKALCN 141A28D41EDEC997 rsbs_bacsu RsbT antagonist protein RsbS rsbS ycxS BSU04680 Stressosome protein RsbS protein protein bacsu Bacillus subtilis MRHPKIPILKLYNCLLVSIQWELDDQTALTFQEDLLNKIYETGANGVVIDLTSVDMIDSFIAKVLGDVITMSKLMGAKVVLTGIQPAVAVTLIELGIALEEIETALDLEQGLETLKRELGE 64BF3EF7D4A81CDA hprk_bacsu HPr kinase/phosphorylase ptsK yvoB HPr kinase/phosphatase HPr(Ser) kinase/phosphorylase BSU35000 hprK protein protein bacsu Bacillus subtilis MAKVRTKDVMEQFNLELISGEEGINRPITMSDLSRPGIEIAGYFTYYPRERVQLLGKTELSFFEQLPEEEKKQRMDSLCTDVTPAIILSRDMPIPQELIDASEKNGVPVLRSPLKTTRLSSRLTNFLESRLAPTTAIHGVLVDIYGVGVLITGKSGVGKSETALELVKRGHRLVADDCVEIRQEDQDTLVGNAPELIEHLLEIRGLGIINVMTLFGAGAVRSNKRITIVMNLELWEQGKQYDRLGLEEETMKIIDTEITKLTIPVRPGRNLAVIIEVAAMNFRLKRMGLNAAEQFTNKLADVIEDGEQEE C44ABD220881EC31 yvek_bacsu Uncharacterized protein YveK yveK BSU34370 protein protein bacsu Bacillus subtilis MNENMSFKELYAIVRHRFVLILLITIGVTLIMGFVQFKVISPTYQASTQVLVHESDGEENSNLSDIQRNLQYSSTFQSIMKSTALMEEVKAELHLSESASSLKGKVVTSSENESEIINVAVQDHDPAKAAEIANTLVNKFEKEVDERMNVQGVHILSEAKASESPMIKPARLRNMVMAFGAAVMGGITLAFFLHFLDDTCKSARQLSERTGLPCLGSVPDVHKGRNRGIKHFGE C1C6703E49B50812 ywqc_bacsu Probable capsular polysaccharide biosynthesis protein YwqC ywqC BSU36260 protein protein bacsu Bacillus subtilis MGESTSLKEILSTLTKRILLIMIVTAAATAAGGLISFFALTPIYENSTQILVNQSKNERKEVQFNDVQTNLQLINTYNVIIKSPAILDEVIKEMGLSMTSQELNDKITVSSEQDSQVVNISVRDENAETAAHIANTIASVFQDKITSIMNVDNVSILSKAEVSEHPSPVSPKPLLNIAIAFAAGLAGSIGLAFLLEHLDNTIKSEEQLESLLDIPVLGTVSTIANEQKTAKTLQGFQSEKTGSGHFGA 95DF89C01682FBED rsbw_bacsu Serine-protein kinase RsbW rsbW Anti-sigma-B factor Sigma-B negative effector RsbW BSU04720 protein protein bacsu Bacillus subtilis MKNNADYIEMKVPAQPEYVGIIRLTLSGVASRMGYTYDEIEDLKIAVSEACTNAVQHAYKEDKNGEVSIRFGVFEDRLEVIVADEGDSFDFDQKQQDLGPYTPSHTVDQLSEGGLGLYLMETLMDEVRVQNHSGVTVAMTKYLNGERVDHDTTIKNYETN A698AA3114646DA0 hpr_bacsu HTH-type transcriptional regulator Hpr hpr catA scoC BSU09990 Protease production regulatory protein Hpr protein protein bacsu Bacillus subtilis MNRVEPPYDVKEALVFTQKMAQLSKALWKSIEKDWQQWLKPYDLNINEHHILWIAYQLNGASISEIAKFGVMHVSTAFNFSKKLEERGYLRFSKRLNDKRNTYVQLTEEGTEVFWSLLEEFDPTRNAVFKGSQPLYHLFGKFPEVAEMMCMIRHIYGDDFMEIFETSLTNIDNDFESVNGKLKKKAKDSAADEPAEELEPVNS 26F46DFBD3E2F5AD pkn1_bacsu Probable serine/threonine-protein kinase YabT yabT BSU00660 protein protein bacsu Bacillus subtilis MMNDALTSLACSLKPGTTIKGKWNGNTYTLRKQLGKGANGIVYLAETSDGHVALKVSDDSLSITSEVNVLKSFSKAQSVTMGPSFFDTDDAYIPSANTKVSFYAMEYIKGPLLLKYVSDKGAEWIPVLMIQLLSSLSVLHQQGWIFGDLKPDNLIVTGPPARIRCIDVGGTTKEGRAIKEYTEFYDRGYWGYGTRKAEPSYDLFAVAMIMINSVHKKEFKKTNQPKEQLRSLIEGNPLLQKYKKALFSALNGDYQSADEMKKDMLDAGQKAAQRKQPIKASPQPATRQRQQKPRQGKITKTRYTPKQKPAKSGGLFETTLIVISVLALYFAYIIFFLI 71896CE0A0A03470 ywqe_bacsu Tyrosine-protein phosphatase YwqE ywqE BSU36240 protein protein bacsu Bacillus subtilis MIDIHCHILPAMDDGAGDSADSIEMARAAVRQGIRTIIATPHHNNGVYKNEPAAVREAADQLNKRLIKEDIPLHVLPGQEIRIYGEVEQDLAKRQLLSLNDTKYILIEFPFDHVPRYAEQLFYDLQLKGYIPVIAHPERNREIRENPSLLYHLVEKGAASQITSGSLAGIFGKQLKAFSLRLVEANLIHFVASDAHNVKTRNFHTQEALYVLEKEFGSELPYMLTENAELLLRNQTIFRQPPQPVKRRKLFGFF E2625AE51E0E9A94 rsbv_bacsu Anti-sigma-B factor antagonist rsbV Anti-anti-sigma-B factor BSU04710 protein protein bacsu Bacillus subtilis MNINVDVKQNENDIQVNIAGEIDVYSAPVLREKLVPLAEQGADLRICLKDVSYMDSTGLGVFVGTFKMVKKQGGSLKLENLSERLIRLFDITGLKDIIDISAKSEGGVQ 12CB03E940463F2C ywqd_bacsu Tyrosine-protein kinase YwqD ywqD BSU36250 protein protein bacsu Bacillus subtilis MALRKNRGSRMQRNVIAMTEPKSLNSEQYRTIRTNIEFASVDRQMKSVMITSACPGEGKSTTAANLAVVFAQQGKKVLLIDADLRKPTVHTAFFLENTVGLTSVLLKKSSMEQAVQASNEKHLDVLTSGPIPPNPAELLSSKWMKELAYEACAAYDMVIFDTPPILAVADAQILGNVADGSVLVISSGKTEKEQAAKAKEALATCKSKLLGAIMNGKKLSKHSEYGYYGTKDNFMQK 40BE35B5E3FF97D7 ybdm_bacsu Probable serine/threonine-protein kinase YbdM ybdM BSU02030 protein protein bacsu Bacillus subtilis MALKLLKKLLFDRPLKNGVILNHQYKIEECLGMGGYGLVYLCTDILAQTPYVLKQLRPTKAKKEKEKVRFQQEIKLLKNIHHPQIPGFIDEFIIDGQAYYVMQFIEGENIEELLFFRKQPFTELMALQLISQLLEIIEYLHDRLIFHSDIRTPNIIINDGRLCLIDFGLAKQLTPEEMEEIKVRKQDDFFDLGETLLFLLYSQYKGKKKKNGTWLEELTLTKEVTLLLKRLLGIEEEYQHTASIREDLNRAIQSVT 2F9750DB23295343 rsbt_bacsu Serine/threonine-protein kinase RsbT rsbT ycxT Switch protein/serine kinase BSU04690 Anti-sigma-B factor RsbT protein protein bacsu Bacillus subtilis MNDQSCVRIMTEWDIVAARQLGRNVAKELGFGTVDQARITTAISELARNIYLYAGKGQIGIEQVADRGKKGLKIIAEDQGPGIPDIRKVMEDGFSTSGGLGAGLPGVKRLMDEFSLNSVAGEGTEIQAIKWLR B9B5CF507F747F7B sp2aa_bacsu Anti-sigma F factor antagonist spoIIAA Stage II sporulation protein AA BSU23470 protein protein bacsu Bacillus subtilis MSLGIDMNVKESVLCIRLTGELDHHTAETLKQKVTQSLEKDDIRHIVLNLEDLSFMDSSGLGVILGRYKQIKQIGGEMVVCAISPAVKRLFDMSGLFKIIRFEQSEQQALLTLGVAS 2333E74207568AC2 prkc_bacsu Serine/threonine-protein kinase PrkC yloP BSU15770 prkC protein protein bacsu Bacillus subtilis MLIGKRISGRYQILRVIGGGGMANVYLAEDIILDREVAIKILRFDYANDNEFIRRFRREAQSASSLDHPNIVSIYDLGEEDDIYYIVMEYVEGMTLKEYITANGPLHPKEALNIMEQIVSAIAHAHQNQIVHRDIKPHNILIDHMGNIKVTDFGIATALSSTTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGRIPFDGESAVSIALKHLQAETPSAKRWNPSVPQSVENIILKATAKDPFHRYETAEDMEADIKTAFDADRLNEKRFTIQEDEEMTKAIPIIKDEELAKAAGEKEAEVTTAQENKTKKNGKRKKWPWVLLTICLVFITAGILAVTVFPSLFMPKDVKIPDVSGMEYEKAAGLLEKEGLQVDSEVLEISDEKIEEGLMVKTDPKADTTVKEGATVTLYKSTGKAKTEIGDVTGQTVDQAKKALKDQGFNHVTVNEVNDEKNAGTVIDQNPSAGTELVPSEDQVKLTVSIGPEDITLRDLKTYSKEAASGYLEDNGLKLVEKEAYSDDVPEGQVVKQKPAAGTAVKPGNEVEVTFSLGPEKKPAKTVKEKVKIPYEPENEGDELQVQIAVDDADHSISDTYEEFKIKEPTERTIELKIEPGQKGYYQVMVNNKVVSYKTIEYPKDE 9653AB5CFBAA7900 rsbt-ybdm-1 2 17 enzyme enzyme unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 18 enzyme target enzyme target substrate substrate unspecified role unspecified role P42411:p.Asn49Ala mutation mutation certain certain sequence position certain certain certain sequence position certain N A Wed May 02 18:56:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn49ala' Tue Jun 30 16:48:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn49Ala' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 P42411 OPhosRes rsbt-yabt 2 18 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P42411:p.Asn49Ala mutation mutation certain certain sequence position certain certain certain sequence position certain N A Wed May 02 17:19:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn49ala' Wed May 02 18:14:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn49ala' Tue Jun 30 16:10:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn49Ala' ecoli Escherichia coli (strain K12) 13 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 P42411 OPhosRes prkc-rsbt 2 20 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 18 enzyme target enzyme target substrate substrate unspecified role unspecified role P42411:p.Asn49Ala mutation mutation certain certain sequence position certain certain certain sequence position certain N A Wed May 02 19:39:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn49ala' Tue Jun 30 19:08:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn49Ala' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 P42411 OPhosRes hprk-ybdm 2 17 enzyme enzyme unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 enzyme target enzyme target substrate substrate unspecified role unspecified role O34483:p.Lys159Met mutation mutation certain certain sequence position certain certain certain sequence position certain K M Wed May 02 14:38:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys159met' Tue Jun 30 17:57:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys159Met' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 O34483 OPhosRes hprk-prkc 2 8 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O34483:p.Lys159Met mutation mutation certain certain sequence position certain certain certain sequence position certain K M Wed May 02 15:38:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys159met' Tue Jun 30 17:19:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys159Met' ecoli Escherichia coli (strain K12) 20 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 O34483 OPhosRes hprk-ywqd 2 8 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O34483:p.Lys159Met mutation mutation certain certain sequence position certain certain certain sequence position certain K M Wed May 02 16:32:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys159met' Tue Jun 30 19:05:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys159Met' ecoli Escherichia coli (strain K12) 16 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 O34483 OPhosRes rsbw-ywqd 2 16 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 enzyme target enzyme target substrate substrate unspecified role unspecified role P17904:p.Asn53Ala mutation mutation certain certain sequence position certain certain certain sequence position certain N A Wed May 02 20:14:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn53ala' Tue Jun 30 19:40:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn53Ala' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 P17904 OPhosRes rsbw-yabt 2 13 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P17904:p.Asn53Ala mutation mutation certain certain sequence position certain certain certain sequence position certain N A Wed May 02 15:12:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn53ala' Tue Jun 30 18:11:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn53Ala' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 P17904 OPhosRes rsbw-prkc 2 11 enzyme target enzyme target substrate substrate unspecified role unspecified role P17904:p.Asn53Ala mutation mutation certain certain sequence position certain certain certain sequence position certain N A Wed May 02 17:44:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn53ala' Tue Jun 30 17:05:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn53Ala' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 20 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 P17904 OPhosRes rsbw-ybdm 2 17 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 enzyme target enzyme target substrate substrate unspecified role unspecified role P17904:p.Asn53Ala mutation mutation certain certain sequence position certain certain certain sequence position certain N A Wed May 02 18:49:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn53ala' Tue Jun 30 16:04:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn53Ala' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 P17904 OPhosRes ybdm-ywqd 2 17 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 16 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P96716:p.Lys59Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 19:53:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys59asp' Tue Jun 30 18:34:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys59Asp' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 P96716 OPhosRes yabt-ywqd 2 16 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P96716:p.Lys59Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 18:10:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys59asp' Tue Jun 30 16:43:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys59Asp' ecoli Escherichia coli (strain K12) 13 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 P96716 OPhosRes hprk-ywqd 2 16 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P96716:p.Lys59Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 14:35:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys59asp' Tue Jun 30 20:00:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys59Asp' ecoli Escherichia coli (strain K12) 8 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 P96716 OPhosRes hprk-ywqd-1 2 16 enzyme enzyme unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate unspecified role unspecified role P37562:p.Lys55Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 16:22:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys55asp' Tue Jun 30 17:46:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys55Asp' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 P37562 OPhosRes yabt-prkc 2 13 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P37562:p.Lys55Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 19:30:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys55asp' Tue Jun 30 16:43:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys55Asp' ecoli Escherichia coli (strain K12) 20 enzyme enzyme unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 P37562 OPhosRes ybdm-yabt 2 17 enzyme enzyme unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate unspecified role unspecified role P37562:p.Lys55Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 18:03:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys55asp' Tue Jun 30 15:47:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys55Asp' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 P37562 OPhosRes prkc-ybdm 2 20 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O34507:p.Lys40Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 18:06:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys40asp' Tue Jun 30 19:47:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys40Asp' ecoli Escherichia coli (strain K12) 17 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 O34507 OPhosRes hprk-prkm 2 17 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O31435:p.Lys54Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 18:33:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys54asp' Tue Jun 30 16:33:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys54Asp' ecoli Escherichia coli (strain K12) 8 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 O31435 OPhosRes prkc-yabt-1 2 13 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 20 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified O34507:p.Lys40Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 15:22:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys40asp' Tue Jun 30 15:59:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys40Asp' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 O34507 OPhosRes spoiiab-prkc 2 6 enzyme target enzyme target substrate substrate unspecified role unspecified role P10728:p.Asn50Ala mutation mutation certain certain sequence position certain certain certain sequence position certain N A Wed May 02 15:44:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn50ala' Tue Jun 30 16:55:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn50Ala' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 13 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 P10728 OPhosRes spoiiab-yabt 2 6 enzyme target enzyme target substrate substrate unspecified role unspecified role P10728:p.Asn50Ala mutation mutation certain certain sequence position certain certain certain sequence position certain N A Wed May 02 19:02:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn50ala' Tue Jun 30 18:20:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn50Ala' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 20 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 P10728 OPhosRes ywqd-spoiiab 2 6 enzyme target enzyme target substrate substrate unspecified role unspecified role P10728:p.Asn50Ala mutation mutation certain certain sequence position certain certain certain sequence position certain N A Wed May 02 16:12:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn50ala' Tue Jun 30 17:54:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn50Ala' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 16 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 P10728 OPhosRes spoiiab-ybdm-1 2 17 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P10728:p.Asn50Ala mutation mutation certain certain sequence position certain certain certain sequence position certain N A Wed May 02 19:12:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn50ala' Tue Jun 30 20:23:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn50Ala' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 P10728 OPhosRes ybdm-ybdm-1 2 17 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified region strep tag strep tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O31435:p.Lys54Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 14:41:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys54asp' Tue Jun 30 15:56:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys54Asp' ecoli Escherichia coli (strain K12) 17 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 2B O31435 OPhosRes yabt-yabt-1 2 13 enzyme target enzyme target substrate substrate unspecified role unspecified role P37562:p.Lys55Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 14:47:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys55asp' Tue Jun 30 17:21:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys55Asp' region strep tag strep tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 13 enzyme enzyme unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 2B P37562 OPhosRes prkc-prkc 2 20 enzyme enzyme unspecified role unspecified role region strep tag strep tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O34507:p.Lys40Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 19:25:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys40asp' Tue Jun 30 17:16:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys40Asp' ecoli Escherichia coli (strain K12) 20 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 2B O34507 OPhosRes rsbv-rsbw 2 15 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 enzyme enzyme unspecified role unspecified role P17904:p.Asn53Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain N A Wed May 02 19:29:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn53ala' Tue Jun 30 19:50:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn53Ala' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 2A P17903 OPhosRes hpr-hprk 2 12 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O34483:p.Gln53Met mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Q M Wed May 02 16:06:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln53met' Tue Jun 30 16:13:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln53Met' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 2A P11065 OPhosRes spoiiaa-spoiiab-5 2 19 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 enzyme enzyme unspecified role unspecified role P10728:p.Asn50Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain N A Wed May 02 15:47:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn50ala' Tue Jun 30 19:40:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn50Ala' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 2A P10727 OPhosRes rsbs-rsbt 2 7 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 18 enzyme enzyme unspecified role unspecified role P42411:p.Asn49Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain N A Wed May 02 20:29:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn49ala' Tue Jun 30 20:12:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn49Ala' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 2A P42410 OPhosRes yabt-ywqd-2 4 16 enzyme enzyme unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P37562:p.Lys55Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 15:55:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys55asp' Tue Jun 30 17:36:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys55Asp' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 4 P37562 tyr-28 OPTyr P37562 tyr-92 OPTyr P37562 tyr-254 OPTyr yabt-ywqd-3 2 20 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 16 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified P96716:p.Lys59Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 18:32:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys59asp' Tue Jun 30 17:59:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys59Asp' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 P96716 OPhosRes prkc-ywqd 4 16 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified P96716:p.Lys59Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 14:37:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys59asp' Tue Jun 30 20:03:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys59Asp' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 20 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 6 P96716 ser-223 OPSer prkc-ybdm-1 2 20 enzyme enzyme unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 17 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O31435:p.Lys54Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 17:36:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys54asp' Tue Jun 30 16:55:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys54Asp' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 8B O31435 OPhosRes prkc-ybdm-2 4 17 enzyme target enzyme target substrate substrate unspecified role unspecified role O31435:p.Lys54Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 15:41:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys54asp' Tue Jun 30 18:00:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys54Asp' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 20 enzyme enzyme unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 5 O31435 Thr-213 OPThr O31435 Thr-241 OPThr Ser-243 OPSer hprk-ybdm-1 4 17 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified O31435:p.Lys54Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 18:27:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys54asp' Tue Jun 30 19:41:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys54Asp' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 5 O31435 ser-148 OPSer spoiiab-ybdm-2 4 6 enzyme target enzyme target substrate substrate unspecified role unspecified role P10728:p.Asn50Ala mutation mutation certain certain sequence position certain certain certain sequence position certain N A Wed May 02 14:53:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn50ala' Tue Jun 30 15:41:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn50Ala' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 17 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 7 P10728 ser-13 OPSer hprk-ybdm-2 4 17 enzyme enzyme unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O34483:p.Lys159Met mutation mutation certain certain sequence position certain certain certain sequence position certain K M Wed May 02 20:00:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys159met' Tue Jun 30 17:55:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys159Met' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 8 O34483 ser-160 OPSer O34483 thr-296 OPThr ywqd-1 2 16 putative self putative self putative self putative self purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P96716:p.Lys59Met mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K M Wed May 02 19:55:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys59met' Tue Jun 30 17:08:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys59Met' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true Figs. 2B, 3 Trans-autophosphorylation could not be detected for this kinase. P96716 OPhosRes ybdm-2 2 17 putative self putative self putative self putative self purified purified O31435:p.Lys54Asp mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K D Wed May 02 19:02:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys54asp' Tue Jun 30 17:28:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys54Asp' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true Figs. 2B, 3 O31435 OPhosRes ywqd-ywqc-1 3 16 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Fig. 1A ywqc-ywqd-3 3 16 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association Fig. 1A ywqc-yvel-1 3 10 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Fig. 1B yvel-ywqc-1 3 10 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Fig. 1C yvek-ywqc-1 3 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Fig. 1C yvel-ywqd 3 16 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Fig. 1C yvel-yabt-1 3 5 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey physical association physical association Fig. 1C yabt-ywqc-1 3 10 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait physical association physical association Fig. 1D yabt-ywqe-1 3 13 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association Fig. 1D rsbt-ybdm-2 3 18 unspecified role unspecified role bait bait 17 unspecified role unspecified role prey prey physical association physical association Fig. 1E rsbt-ywqd-1 3 16 unspecified role unspecified role prey prey P96716:p.Asp81Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain D A Wed May 02 19:02:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp81ala' Tue Jun 30 18:40:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp81Ala' 18 unspecified role unspecified role bait bait physical association physical association Fig. 1E rsbt-ywqe-1 3 14 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait physical association physical association Fig. 1E yabt-ybdm 2 17 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O31435:p.Lys54Asp mutation mutation certain certain sequence position certain certain certain sequence position certain K D Wed May 02 19:58:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys54asp' Tue Jun 30 19:11:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys54Asp' ecoli Escherichia coli (strain K12) 13 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 3 O31435 OPhosRes yabt-1 2 13 putative self putative self putative self putative self region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P37562:p.Lys55Asp mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K D Wed May 02 17:14:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys55asp' Tue Jun 30 18:57:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys55Asp' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true Fig. 3, 2B. P37562 OPhosRes prkc-4 2 20 putative self putative self putative self putative self O34507:p.Lys40Asp mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain K D Wed May 02 15:53:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys40asp' Tue Jun 30 18:02:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys40Asp' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true Fig. 3, 2B. O34507 OPhosRes hpr-hprk-1 1 12 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 9 O34507 OPhosRes ybdm-1 1 17 putative self putative self putative self putative self purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true Fig. 9 O31435 OPhosRes hpr-hprk-2 1 12 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 17 inhibitor inhibitor unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 9 O34507 OPhosRes The presence of PrkD in the phosphorylation assay significantly diminished the capacity of HprK/P to phosphorylate HPr. hprk-ybdm-3 1 8 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 17 enzyme enzyme unspecified role unspecified role purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 9 O34483 OPhosRes