MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it VP16 fusion induces the multiple-knockout phenotype of redundant transcriptional repressors partly by Med25-independent mechanisms in Arabidopsis. VP16 fusion induces the multiple-knockout phenotype of redundant transcriptional repressors partly by Med25-independent mechanisms in Arabidopsis. FEBS Lett. (0014-5793) 2014 imex curation Fujiwara S., Sakamoto S., Kigoshi K., Suzuki K., Ohme-Takagi M. fujiwara-s@aist.go.jp 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions nicbe Nicotiana benthamiana anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western fujiwara-s@aist.go.jp Accepted 2014-AUG-06 by MARTA 2014 imex curation VP16 fusion induces the multiple-knockout phenotype of redundant transcriptional repressors partly by Med25-independent mechanisms in Arabidopsis. VP16 fusion induces the multiple-knockout phenotype of redundant transcriptional repressors partly by Med25-independent mechanisms in Arabidopsis. FEBS Lett. (0014-5793) 2014 imex curation Fujiwara S., Sakamoto S., Kigoshi K., Suzuki K., Ohme-Takagi M. fujiwara-s@aist.go.jp 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions nicbe Nicotiana benthamiana bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence fujiwara-s@aist.go.jp Accepted 2014-AUG-06 by MARTA 2014 imex curation vp16_hhv11 Tegument protein VP16 Vmw65 ICP25 Alpha-TIF Alpha trans-inducing protein UL48 protein protein hhv11 Human herpesvirus 1 (strain 17) (HHV-1) Human herpes simplex virus 1 HHV-1 MDLLVDELFADMNADGASPPPPRPAGGPKNTPAAPPLYATGRLSQAQLMPSPPMPVPPAALFNRLLDDLGFSAGPALCTMLDTWNEDLFSALPTNADLYRECKFLSTLPSDVVEWGDAYVPERTQIDIRAHGDVAFPTLPATRDGLGLYYEALSRFFHAELRAREESYRTVLANFCSALYRYLRASVRQLHRQAHMRGRDRDLGEMLRATIADRYYRETARLARVLFLHLYLFLTREILWAAYAEQMMRPDLFDCLCCDLESWRQLAGLFQPFMFVNGALTVRGVPIEARRLRELNHIREHLNLPLVRSAATEEPGAPLTTPPTLHGNQARASGYFMVLIRAKLDSYSSFTTSPSEAVMREHAYSRARTKNNYGSTIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG DIP protein P06492 original sequence version: 97 DIP protein P06492 original sequence version: 93 DIP protein P06492 original sequence version: 107 mint Responsible for transcriptional activation of immediate early promoters (alpha genes). Activation requires the formation of a heteromeric complex with the host cell factor. These two proteins then assemble with the octamer motif-binding protein Oct-1 on the cis-acting target sequence: 5'-TAATGARAT-3' 8DDDEDEDB2A699D3 med25_arath Mediator of RNA polymerase II transcription subunit 25 MED25 MED25_1 PFT1 Phytochrome and flowering time 1 protein Phytochrome and flowering time regulatory protein 1 F2J7.4 At1g25540 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSSEVKQLIVVAEGTAALGPYWQTIVSDYLEKIIRSFCGSELNGERNPVSTVELSLVIFNSHGSYCACLVQRSGWTRDVDIFLHWLSSIQFGGGGFNEVATAEGLAEALMMFSPPSGQAQPSNDLKRHCILITASNPHILPTPVYRPRLQNVERNENGDAQAESRLSDAETVASYFAKCSVSLSVVCPKQLPTIRALYNAGKPNQQSADLSIDTAKNTFYLVLISENFVEACAALSHSATNLPQTQSPVKVDRATVAPSIPVTGQPPAPVSSANGPIQNRQPVSVGPVPTATVKVEPSTVTSMAPVPSFPHIPAVARPATQAIPSIQTSSASPVSQDMVSNAENAPDIKPVVVSGMTPPLRTGPPGGANVNLLNNLSQVRQVMSSAALAGAASSVGQSAVAMHMSNMISTGMATSLPPSQTVFSTGQQGITSMAGSGALMGSAQTGQSPGPNNAFSPQTTSNVASNLGVSQPMQGMNQGSHSGAMMQGGISMNQNMMSGLGQGNVSSGTGGMMPTPGVGQQAQSGIQQLGGSNSSAPNMQLSQPSSGAMQTSQSKYVKVWEGNLSGQRQGQPVLITRLEGYRSASASDSLAANWPPTMQIVRLISQDHMNNKQYVGKADFLVFRAMSQHGFLGQLQDKKLCAVIQLPSQTLLLSVSDKACRLIGMLFPGDMVVFKPQIPNQQQQQQQQLHQQQQQQQQIQQQQQQQQHLQQQQMPQLQQQQQQHQQQQQQQHQLSQLQHHQQQQQQQQQQQQQHQLTQLQHHHQQQQQASPLNQMQQQTSPLNQMQQQTSPLNQMQQQQQPQQMVMGGQAFAQAPGRSQQGGGGGQPNMPGAGFMG 9A9C81527BE4F384 vp16-med25 1 VP16 3 anti tag western anti tag western blot anti tag western anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Med25 4 anti tag western anti tag western blot anti tag western anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f4a To test whether plant Med25 also interacts with VP16 in vivo, we performed immunoprecipitation assays using the N. benthamiana system to co-express the tagged Arabidopsis proteins (Figure 4A). FLAG-tagged Med25 (FLAG-Med25) was co-expressed with GFP-FLC, GFP-FLC-VP16, GFP-VP16, or GFP, and the protein complex including the GFP-tagged proteins was immunoprecipitated with an α-GFP antibody. FLAG-Med25 was co-immunoprecipitated with the VP16-fused proteins, GFP-FLC-VP16 and GFP-VP16, but not with GFP or GFP-FLC without VP16 fusion, indicating that VP16 directly or indirectly interacts with Arabidopsis Med25 in vivo. vp16-med25-1 2 VP16 3 tag fluorescence tag visualisation by fluorescence tag fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain yfp tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Med25 4 tag fluorescence tag visualisation by fluorescence tag fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell yfp tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association f4b To test whether plant Med25 also interacts with VP16 in vivo, we performed immunoprecipitation assays using the N. benthamiana system to co-express the tagged Arabidopsis proteins (Figure 4A). FLAG-tagged Med25 (FLAG-Med25) was co-expressed with GFP-FLC, GFP-FLC-VP16, GFP-VP16, or GFP, and the protein complex including the GFP-tagged proteins was immunoprecipitated with an α-GFP antibody. FLAG-Med25 was co-immunoprecipitated with the VP16-fused proteins, GFP-FLC-VP16 and GFP-VP16, but not with GFP or GFP-FLC without VP16 fusion, indicating that VP16 directly or indirectly interacts with Arabidopsis Med25 in vivo.