IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Polo-like kinase 1 licenses CENP-A deposition at centromeres. Polo-like kinase 1 licenses CENP-A deposition at centromeres. Cell (0092-8674) 2014 imex curation McKinley K.L., Cheeseman I.M. Additional detail on constructs obtained from the author icheese@wi.mit.edu Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant McKinley K.L., Cheeseman I.M. Additional detail on constructs obtained from the author icheese@wi.mit.edu Accepted 2015-JUL-21 by PPORRAS Cell (0092-8674) 2014 Only protein-protein interactions imex curation Mock publication and experiment for abstract interactions that are not interaction evidences. Mock publication and experiment for abstract interactions that are not interaction evidences. unspecified method Polo-like kinase 1 licenses CENP-A deposition at centromeres. Polo-like kinase 1 licenses CENP-A deposition at centromeres. Cell (0092-8674) 2014 imex curation McKinley K.L., Cheeseman I.M. Additional detail on constructs obtained from the author icheese@wi.mit.edu Only protein-protein interactions ecoli Escherichia coli (strain K12) pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie McKinley K.L., Cheeseman I.M. Additional detail on constructs obtained from the author icheese@wi.mit.edu Accepted 2015-JUL-21 by PPORRAS Cell (0092-8674) 2014 Only protein-protein interactions imex curation Polo-like kinase 1 licenses CENP-A deposition at centromeres. Polo-like kinase 1 licenses CENP-A deposition at centromeres. Cell (0092-8674) 2014 imex curation McKinley K.L., Cheeseman I.M. Additional detail on constructs obtained from the author icheese@wi.mit.edu Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay ms participant Identification by mass spectrometry ms participant McKinley K.L., Cheeseman I.M. Additional detail on constructs obtained from the author icheese@wi.mit.edu Accepted 2015-JUL-21 by PPORRAS Cell (0092-8674) 2014 Only protein-protein interactions imex curation Polo-like kinase 1 licenses CENP-A deposition at centromeres. Polo-like kinase 1 licenses CENP-A deposition at centromeres. Cell (0092-8674) 2014 imex curation McKinley K.L., Cheeseman I.M. Additional detail on constructs obtained from the author icheese@wi.mit.edu Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot McKinley K.L., Cheeseman I.M. Additional detail on constructs obtained from the author icheese@wi.mit.edu Accepted 2015-JUL-21 by PPORRAS Cell (0092-8674) 2014 Only protein-protein interactions imex curation m18bp_mouse Mis18-binding protein 1 Mis18bp1 Kiaa1903 Knl2 M18bp1 Kinetochore-associated protein KNL-2 homolog protein protein mouse Mus musculus Mouse MIVTPLKHSGIHLSSGTLQRRNMPLDAVFIDSIPSGTLTPLKDLVKYQKSSLKVNGHKKNQLLEIRTSNNKDLFQSTMLSEATLPNSSLDISVIKPSMDRLRNEMIYESPGKIFQRMKAKVQRDKQEQLTRSSSMLGSPQGEHTKDFPPNTDKKAQLQQTYICEEKQTSVQSNDPSLGDPPILNQEQKNVSASCISKKALTRAQFGGQVLHSKESPVRITVSKKNTFVLGGIDCTYEKFENTDVNTISSLCVPIKNHSQSITSDNDVTTERTAKEDITEPNEEMMSRRTILQDPIKNTSKIKRSSPRPNLTLSGRSQRKCTKLETVVKEVKKYQAVHLQEWMIKVINNNTAICVEGKLVDMTDVYWHSNVIIERIKHNELRTLSGNIYILKGLIDSVSMKEAGYPCYLTRKFMFGFPHNWKEHIDKFLEQLRAEKKNKTRQETARVQEKQKSKKKDAEDKETYVLQKASITYDLNDNSLERTEVPTDPLNSLEQPTSGKERRHPLLSQKRAYVLITPLRNKKLIEQRCIDYSLSIEGISDFFKAKHQEESDSDIHGTPSSTSKSQETFEHRVGFEGNTKEDCNECDIITARHIQIPCPKSKQMLTNDFMKKNKLPSKLQKTENQIGVSQYCRSSSHLSSEENEVEIKSRTRARNTKERLNRERENTNHITKDILLISETEGERACYITPKRPRSCYITPKRPRSSAKESHYKSAVSKDFLTEGKASDRTSRQLLDHLPGLTDDEEWSEQELQKLHCAFTSLPKHKPGFWSDVAMAVGSRTADECQKKYTEEPQGQGSRKHGSKKKQANKVQNGEKDSADAKTIKITAKVGTLKRKRQMRDCLEHLAKDNHDDFFTATPLQKQRIQLPSFQYSQDDDFLLDMDRDPASPSSIITSPLRSTTPQCQHFSPSMLAAIERNNCDRYVYQMQKNAKKYGKSNGGLVWGNIRKKTVKTDLSSPPPTRKALFNKDLGKNTDISKYFIDDTESDEEEKDYYFSNSD E0194CC994543C8F m18bp_human Mis18-binding protein 1 MIS18BP1 C14orf106 KIAA1903 KNL2 M18BP1 Kinetochore-associated protein KNL-2 homolog P243 protein protein human Homo sapiens Human MIATPLKHSRIYLPPEASSQRRNLPMDAIFFDSIPSGTLTPVKDLVKYQNSSLKLNDHKKNQFLKMTTFNNKNIFQSTMLTEATTSNSSLDISAIKPNKDGLKNKANYESPGKIFLRMKEKVLRDKQEQPSRNSSLLEPQKSGNNETFTPNRVEKKKLQHTYLCEEKENNKSFQSDDSSLRASVQGVPLESSNNDIFLPVKQKIQCQQEKKAPLHNLTYELPTLNQEQENFLAVEARNKTLTRAQLAKQIFHSKESIVATTKSKKDTFVLESVDSADEQFQNTNAETLSTNCIPIKNGSLLMVSDSERTTEGTSQQKVKEGNGKTVPGETGLPGSMKDTCKIVLATPRLHITIPRRSKRNISKLSPPRIFQTVTNGLKKNQVVQLQEWMIKSINNNTAICVEGKLIDVTNIYWHSNVIIERIEHNKLRTISGNVYILKGMIDQISMKEAGYPNYLIRKFMFGFPENWKEHIDNFLEQLRAGEKNREKTKQKQKTGRSVRDIRKSMKNDARENQTDTAQRATTTYDFDCDNLELKSNKHSESPGATELNMCHSNCQNKPTLRFPDDQVNNTIQNGGGDDLSNQELIGKKEYKMSSKKLKIGERTNERIIKSQKQETTEELDVSIDILTSREQFFSDEERKYMAINQKKAYILVTPLKSRKVIEQRCMRYNLSAGTIKAVTDFVIPECQKKSPISKSMGTLENTFEGHKSKNKEDCDERDLLTVNRKIKISNLEKEQMLTSDFKKNTRLLPKLKKIENQVAMSFYKHQSSPDLSSEESETEKEIKRKAEVKKTKAGNTKEAVVHLRKSTRNTSNIPVILEPETEESENEFYIKQKKARPSVKETLQKSGVRKEFPITEAVGSDKTNRHPLECLPGLIQDKEWNEKELQKLHCAFASLPKHKPGFWSEVAAAVGSRSPEECQRKYMENPRGKGSQKHVTKKKPANSKGQNGKRGDADQKQTIKITAKVGTLKRKQQMREFLEQLPKDDHDDFFSTTPLQHQRILLPSFQDSEDDDDILPNMDKNPTTPSSVIFPLVKTPQCQHVSPGMLGSINRNDCDKYVFRMQKYHKSNGGIVWGNIKKKLVETDFSTPTPRRKTPFNTDLGENSGIGKLFTNAVESLDEEEKDYYFSNSDSA 92F2787CD4687059 plk1_human Serine/threonine-protein kinase PLK1 Polo-like kinase 1 Serine/threonine-protein kinase 13 PLK1 PLK protein protein human Homo sapiens Human MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS DIP protein P53350 original sequence version: 169 DIP protein P53350 original sequence version: 170 DIP protein P53350 original sequence version: 164 DIP protein P53350 original sequence version: 154 DIP protein P53350 original sequence version: 91 DIP protein P53350 original sequence version: 145 DIP protein P53350 original sequence version: 121 DIP protein P53350 original sequence version: 118 DIP protein P53350 original sequence version: 123 DIP protein P53350 original sequence version: 119 DIP protein P53350 original sequence version: 138 DIP protein P53350 original sequence version: 163 DIP protein P53350 original sequence version: 178 178C2F13C10E8206 ms18a_mouse Protein Mis18-alpha Mis18a protein protein mouse Mus musculus Mouse MSSESPLLEKRLSEDSSRYLRLQKWANMSSADALGLEKERPEEKAAAAENPLVFLCARCRRPLGDSLTWVASQEDTNCILLRSVSCNVSVDKEPKLSKCRDEDGCILEALYCTGCSLSLGYVYRCTPKNLDYKRDLFCLSVEAVESYTLGSSEKQIVSEDKELFNLESRVEIEKSIKQMEEVLTALQKKLREVESKLSLASRGS BBD667250FDF9B0F ms18a_human Protein Mis18-alpha MIS18A FAPP1-associated protein 1 C21orf46 FASP1 C21orf45 protein protein human Homo sapiens Human MAGVRSLRCSRGCAGGCECGDKGKCSDSSLLGKRLSEDSSRHQLLQKWASMWSSMSEDASVADMERAQLEEEAAAAEERPLVFLCSGCRRPLGDSLSWVASQEDTNCILLRCVSCNVSVDKEQKLSKREKENGCVLETLCCAGCSLNLGYVYRCTPKNLDYKRDLFCLSVEAIESYVLGSSEKQIVSEDKELFNLESRVEIEKSLTQMEDVLKALQMKLWEAESKLSFATCKS A07522806C4B6221 ms18b_human Protein Mis18-beta Cancer/testis antigen 86 OIP5 MIS18B Opa-interacting protein 5 protein protein human Homo sapiens Human MAAQPLRHRSRCATPPRGDFCGGTERAIDQASFTTSMEWDTQVVKGSSPLGPAGLGAEEPAAGPQLPSWLQPERCAVFQCAQCHAVLADSVHLAWDLSRSLGAVVFSRVTNNVVLEAPFLVGIEGSLKGSTYNLLFCGSCGIPVGFHLYSTHAALAALRGHFCLSSDKMVCYLLKTKAIVNASEMDIQNVPLSEKIAELKEKIVLTHNRLKSLMKILSEVTPDQSKPEN 0EBD4006193A3106 ms18b_mouse Protein Mis18-beta Oip5 Ms18b Opa-interacting protein 5 homolog protein protein mouse Mus musculus Mouse MATLSRRSLRFLETQSRRDSCDTPFSLTSSMEWDTQVVEGFSPLGSSESKVKASPVDLRLPAWLEPERCAVFHCARCYAVLGDTLHLAWDLSRSLGALAFSKVTNNVVLLEPFLVGIEGFLKSSTYNLLFCNSCGTPVGFHLYSTHAAMAALRGHFCLSSDKMLCYLLKTNAIVNTSEMDFHNVPLPEKIAELKEKIMLMHTRLNSLTGLLKGKSPHQFKQENQQARKQHILGLTASPKIL 63AC2693958E29F4 mis18a-mis18bp1-3 1 10 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey association association Fig. 1C mis18a-mis18bp1-4 3 11 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Supp. Fig. 4A mis18-plk1-1 4 26 enzyme target enzyme target substrate substrate unspecified role unspecified role q9nyp9 region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 enzyme enzyme unspecified role unspecified role ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Supp. Fig. 4B, 4C EBI-9872194 Q6P0N0 ser-93 O-phospho-L-serine EBI-9872194 Q6P0N0 ser-179 O-phospho-L-serine EBI-9872194 Q6P0N0 ser-192 O-phospho-L-serine EBI-9872194 Q6P0N0 ser-218 O-phospho-L-serine EBI-9872194 Q6P0N0 thr-570 O-phospho-L-threonine EBI-9872194 Q6P0N0 ser-622 O-phospho-L-serine EBI-9872194 Q6P0N0 thr-702 O-phospho-L-threonine EBI-9872194 Q6P0N0 ser-1104 O-phospho-L-serine EBI-9872194 Q9NYP9 ser-53 O-phospho-L-serine EBI-9872194 Q9NYP9 ser-54 O-phospho-L-serine EBI-9872194 Q9NYP9 ser-56 O-phospho-L-serine EBI-9872194 Q9NYP9 ser-60 O-phospho-L-serine EBI-9872194 Q9NYP9 ser-204 O-phospho-L-serine EBI-9872194 Q9NYP9 thr-206 O-phospho-L-threonine EBI-9872194 O43482 thr-221 O-phospho-L-threonine EBI-9872194 O43482 ser-225 O-phospho-L-serine P53350 increases activity of EBI-9872194 plk1-mis18 5 8 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pbd region sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) 34 unspecified role unspecified role prey prey q9nyp9 region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 4G mis18_mouse Mis18 complex Mis18a:Mis18b:Mis18bp1 6 unspecified role unspecified role 9 unspecified role unspecified role 12 unspecified role unspecified role physical association physical association mouse Mus musculus Mouse stable complex stable complex biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion Orchestrates the deposition of CENP-A, the centromere-specific histone H3 variant which is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. The redistribution of CENP-A nucleosomes between the two new DNA strands is necessary to maintain the epigenetic mark of the centromere and leads to the dilution of CENP-A nucleosomes. The Mis18 complex localizes to centromeres just prior to the pre-nucleosomal HJURP/CENP-A/H4 complex and is absolutely required for the CENP-A-specific chaperone, Holliday junction recognition protein (HJURP, Q6PG16) to reach the centromeres. CDK phosphorylation of MISBP1 during G2 and mitosis, prior to the metaphase-to-anaphase transition, negatively regulates complex assembly. Plk1 phosphorylation activates Mis18 complex recruitment to the centromeres during G1. Accepted 2014-DEC-01 by BMELDAL mis18_human Mis18 complex MIS18A:MIS18B:MIS18BP1 11 unspecified role unspecified role mis18a binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role 10 unspecified role unspecified role mis18b binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 40 43 physical association physical association human Homo sapiens Human stable complex stable complex biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion Orchestrates the deposition of CENP-A, the centromere-specific histone H3 variant which is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. The redistribution of CENP-A nucleosomes between the two new DNA strands is necessary to maintain the epigenetic mark of the centromere and leads to the dilution of CENP-A nucleosomes. The Mis18 complex localizes to centromeres just prior to the pre-nucleosomal HJURP/CENP-A/H4 complex and is absolutely required for the CENP-A-specific chaperone, Holliday junction recognition protein (HJURP, Q8NCD3) to reach the centromeres. CDK phosphorylation of MISBP1 during G2 and mitosis, prior to the metaphase-to-anaphase transition, negatively regulates complex assembly. Plk1 phosphorylation activates Mis18 complex recruitment to the centromeres during G1. Accepted 2014-DEC-01 by BMELDAL