IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ DJ-1 interacts with RACK1 and protects neurons from oxidative-stress-induced apoptosis. DJ-1 interacts with RACK1 and protects neurons from oxidative-stress-induced apoptosis. Biochem. J. (0264-6021) 2014 imex curation Ma J., Wu R., Zhang Q., Wu J., Lou J., Zheng Z., Ding J.Q., Yuan Z. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. I have a paper copy of the PDF file on the left of my desk should you wish to use it. zqyuan@sun5.ibp.ac.cn 18-Sep-2014: Contacted by IntAct-Help. from Rebecca Foulger Only protein-protein interactions mouse-cerebral_cortical_neuron Mouse cerebral cortical neuron brain-cortex_neuron Cerebral cortical neuron anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ma J., Wu R., Zhang Q., Wu J., Lou J., Zheng Z., Ding J.Q., Yuan Z. Parkinsons - Interactions investigated in the context of Parkinsons disease I have a paper copy of the PDF file on the left of my desk should you wish to use it. zqyuan@sun5.ibp.ac.cn Accepted 2014-SEP-01 by ORCHARD from Rebecca Foulger Biochem. J. (0264-6021) 2014 Only protein-protein interactions imex curation DJ-1 interacts with RACK1 and protects neurons from oxidative-stress-induced apoptosis. DJ-1 interacts with RACK1 and protects neurons from oxidative-stress-induced apoptosis. Biochem. J. (0264-6021) 2014 imex curation Ma J., Wu R., Zhang Q., Wu J., Lou J., Zheng Z., Ding J.Q., Yuan Z. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. I have a paper copy of the PDF file on the left of my desk should you wish to use it. zqyuan@sun5.ibp.ac.cn 18-Sep-2014: Contacted by IntAct-Help. from Rebecca Foulger Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Ma J., Wu R., Zhang Q., Wu J., Lou J., Zheng Z., Ding J.Q., Yuan Z. Parkinsons - Interactions investigated in the context of Parkinsons disease I have a paper copy of the PDF file on the left of my desk should you wish to use it. The authors have provided the identities of the proteins in Figure 1A.(PPP1R12A, PTBP1, SOD1, IQGAP1). zqyuan@sun5.ibp.ac.cn Accepted 2014-SEP-01 by ORCHARD from Rebecca Foulger Biochem. J. (0264-6021) 2014 Only protein-protein interactions imex curation DJ-1 interacts with RACK1 and protects neurons from oxidative-stress-induced apoptosis. DJ-1 interacts with RACK1 and protects neurons from oxidative-stress-induced apoptosis. Biochem. J. (0264-6021) 2014 imex curation Ma J., Wu R., Zhang Q., Wu J., Lou J., Zheng Z., Ding J.Q., Yuan Z. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. I have a paper copy of the PDF file on the left of my desk should you wish to use it. zqyuan@sun5.ibp.ac.cn 18-Sep-2014: Contacted by IntAct-Help. from Rebecca Foulger Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ma J., Wu R., Zhang Q., Wu J., Lou J., Zheng Z., Ding J.Q., Yuan Z. Parkinsons - Interactions investigated in the context of Parkinsons disease I have a paper copy of the PDF file on the left of my desk should you wish to use it. zqyuan@sun5.ibp.ac.cn Accepted 2014-SEP-01 by ORCHARD from Rebecca Foulger Biochem. J. (0264-6021) 2014 Only protein-protein interactions imex curation DJ-1 interacts with RACK1 and protects neurons from oxidative-stress-induced apoptosis. DJ-1 interacts with RACK1 and protects neurons from oxidative-stress-induced apoptosis. Biochem. J. (0264-6021) 2014 imex curation Ma J., Wu R., Zhang Q., Wu J., Lou J., Zheng Z., Ding J.Q., Yuan Z. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. I have a paper copy of the PDF file on the left of my desk should you wish to use it. zqyuan@sun5.ibp.ac.cn 18-Sep-2014: Contacted by IntAct-Help. from Rebecca Foulger Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Ma J., Wu R., Zhang Q., Wu J., Lou J., Zheng Z., Ding J.Q., Yuan Z. Parkinsons - Interactions investigated in the context of Parkinsons disease I have a paper copy of the PDF file on the left of my desk should you wish to use it. zqyuan@sun5.ibp.ac.cn Accepted 2014-SEP-01 by ORCHARD from Rebecca Foulger Biochem. J. (0264-6021) 2014 Only protein-protein interactions imex curation DJ-1 interacts with RACK1 and protects neurons from oxidative-stress-induced apoptosis. DJ-1 interacts with RACK1 and protects neurons from oxidative-stress-induced apoptosis. Biochem. J. (0264-6021) 2014 imex curation Ma J., Wu R., Zhang Q., Wu J., Lou J., Zheng Z., Ding J.Q., Yuan Z. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. I have a paper copy of the PDF file on the left of my desk should you wish to use it. zqyuan@sun5.ibp.ac.cn 18-Sep-2014: Contacted by IntAct-Help. from Rebecca Foulger Only protein-protein interactions mouse-cerebral_cortical_neuron Mouse cerebral cortical neuron brain-cortex_neuron Cerebral cortical neuron confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Ma J., Wu R., Zhang Q., Wu J., Lou J., Zheng Z., Ding J.Q., Yuan Z. Parkinsons - Interactions investigated in the context of Parkinsons disease I have a paper copy of the PDF file on the left of my desk should you wish to use it. zqyuan@sun5.ibp.ac.cn Accepted 2014-SEP-01 by ORCHARD from Rebecca Foulger Biochem. J. (0264-6021) 2014 Only protein-protein interactions imex curation park7_human Parkinson disease protein 7 PARK7 Oncogene DJ1 Parkinsonism-associated deglycase Maillard deglycase Protein DJ-1 Protein/nucleic acid deglycase DJ-1 protein protein human Homo sapiens Human MASKRALVILAKGAEEMETVIPVDVMRRAGIKVTVAGLAGKDPVQCSRDVVICPDASLEDAKKEGPYDVVVLPGGNLGAQNLSESAAVKEILKEQENRKGLIAAICAGPTALLAHEIGFGSKVTTHPLAKDKMMNGGHYTYSENRVEKDGLILTSRGPGTSFEFALAIVEALNGKEVAAQVKAPLVLKD DIP protein Q99497 original sequence version: 102 4B21661B3A76BC67 mypt1_human Protein phosphatase 1 regulatory subunit 12A PPP1R12A MBS MYPT1 Myosin phosphatase-targeting subunit 1 Protein phosphatase myosin-binding subunit protein protein human Homo sapiens Human MKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLKLLHRGADINYANVDGLTALHQACIDDNVDMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGDTPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERIMLRDARQWLNSGHINDVRHAKSGGTALHVAAAKGYTEVLKLLIQAGYDVNIKDYDGWTPLHAAAHWGKEEACRILVDNLCDMEMVNKVGQTAFDVADEDILGYLEELQKKQNLLHSEKRDKKSPLIESTANMDNNQSQKTFKNKETLIIEPEKNASRIESLEQEKVDEEEEGKKDESSCSSEEDEEDDSESEAETDKTKPLASVTNANTSSTQAAPVAVTTPTVSSGQATPTSPIKKFPTTATKISPKEEERKDESPATWRLGLRKTGSYGALAEITASKEGQKEKDTAGVTRSASSPRLSSSLDNKEKEKDSKGTRLAYVAPTIPRRLASTSDIEEKENRDSSSLRTSSSYTRRKWEDDLKKNSSVNEGSTYHKSCSFGRRQDDLISSSVPSTTSTPTVTSAAGLQKSLLSSTSTTTKITTGSSSAGTQSSTSNRLWAEDSTEKEKDSVPTAVTIPVAPTVVNAAASTTTLTTTTAGTVSSTTEVRERRRSYLTPVRDEESESQRKARSRQARQSRRSTQGVTLTDLQEAEKTIGRSRSTRTREQENEEKEKEEKEKQDKEKQEEKKESETSREDEYKQKYSRTYDETYQRYRPVSTSSSTTPSSSLSTMSSSLYASSQLNRPNSLVGITSAYSRGITKENEREGEKREEEKEGEDKSQPKSIRERRRPREKRRSTGVSFWTQDSDENEQEQQSDTEEGSNKKETQTDSISRYETSSTSAGDRYDSLLGRSGSYSYLEERKPYSSRLEKDDSTDFKKLYEQILAENEKLKAQLHDTNMELTDLKLQLEKATQRQERFADRSLLEMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK DIP protein O14974 original sequence version: 95 DIP protein O14974 original sequence version: 141 EA43E9BFF5DA08FF rack1_mouse Receptor of activated protein C kinase 1 p205 Receptor of activated protein kinase C 1 12-3 Gnb2-rs1 Gnb2l1 Guanine nucleotide-binding protein subunit beta-2-like 1 Rack1 Receptor for activated C kinase protein protein mouse Mus musculus Mouse MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR 257F91E369ED2044 ptbp1_human Polypyrimidine tract-binding protein 1 PTBP1 PTB Heterogeneous nuclear ribonucleoprotein I 57 kDa RNA-binding protein PPTB-1 protein protein human Homo sapiens Human MDGIVPDIAVGTKRGSDELFSTCVTNGPFIMSSNSASAANGNDSKKFKGDSRSAGVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIEMNTEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQARAQAALQAVNSVQSGNLALAASAAAVDAGMAMAGQSPVLRIIVENLFYPVTLDVLHQIFSKFGTVLKIITFTKNNQFQALLQYADPVSAQHAKLSLDGQNIYNACCTLRIDFSKLTSLNVKYNNDKSRDYTRPDLPSGDSQPSLDQTMAAAFGLSVPNVHGALAPLAIPSAAAAAAAAGRIAIPGLAGAGNSVLLVSNLNPERVTPQSLFILFGVYGDVQRVKILFNKKENALVQMADGNQAQLAMSHLNGHKLHGKPIRITLSKHQNVQLPREGQEDQGLTKDYGNSPLHRFKKPGSKNFQNIFPPSATLHLSNIPPSVSEEDLKVLFSSNGGVVKGFKFFQKDRKMALIQMGSVEEAVQALIDLHNHDLGENHHLRVSFSKSTI BE12D5EA21537BED park7_mouse Parkinson disease protein 7 homolog Park7 Protein/nucleic acid deglycase DJ-1 Maillard deglycase Parkinsonism-associated deglycase Protein DJ-1 protein protein mouse Mus musculus Mouse MASKRALVILAKGAEEMETVIPVDVMRRAGIKVTVAGLAGKDPVQCSRDVMICPDTSLEDAKTQGPYDVVVLPGGNLGAQNLSESPMVKEILKEQESRKGLIAAICAGPTALLAHEVGFGCKVTTHPLAKDKMMNGSHYSYSESRVEKDGLILTSRGPGTSFEFALAIVEALVGKDMANQVKAPLVLKD 877C825CCA07468F sodc_human Superoxide dismutase [Cu-Zn] Superoxide dismutase 1 SOD1 protein protein human Homo sapiens Human MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ DIP protein P00441 original sequence version: 194 DIP protein P00441 original sequence version: 189 DIP protein P00441 original sequence version: 186 DIP protein P00441 original sequence version: 147 DIP protein P00441 original sequence version: 148 DIP protein P00441 original sequence version: 145 DIP protein P00441 original sequence version: 169 25CA38DA8D564483 iqga1_human Ras GTPase-activating-like protein IQGAP1 IQGAP1 KIAA0051 p195 protein protein human Homo sapiens Human MSAADEVDGLGVARPHYGSVLDNERLTAEEMDERRRQNVAYEYLCHLEEAKRWMEACLGEDLPPTTELEEGLRNGVYLAKLGNFFSPKVVSLKKIYDREQTRYKATGLHFRHTDNVIQWLNAMDEIGLPKIFYPETTDIYDRKNMPRCIYCIHALSLYLFKLGLAPQIQDLYGKVDFTEEEINNMKTELEKYGIQMPAFSKIGGILANELSVDEAALHAAVIAINEAIDRRIPADTFAALKNPNAMLVNLEEPLASTYQDILYQAKQDKMTNAKNRTENSERERDVYEELLTQAEIQGNINKVNTFSALANIDLALEQGDALALFRALQSPALGLRGLQQQNSDWYLKQLLSDKQQKRQSGQTDPLQKEELQSGVDAANSAAQQYQRRLAAVALINAAIQKGVAEKTVLELMNPEAQLPQVYPFAADLYQKELATLQRQSPEHNLTHPELSVAVEMLSSVALINRALESGDVNTVWKQLSSSVTGLTNIEEENCQRYLDELMKLKAQAHAENNEFITWNDIQACVDHVNLVVQEEHERILAIGLINEALDEGDAQKTLQALQIPAAKLEGVLAEVAQHYQDTLIRAKREKAQEIQDESAVLWLDEIQGGIWQSNKDTQEAQKFALGIFAINEAVESGDVGKTLSALRSPDVGLYGVIPECGETYHSDLAEAKKKKLAVGDNNSKWVKHWVKGGYYYYHNLETQEGGWDEPPNFVQNSMQLSREEIQSSISGVTAAYNREQLWLANEGLITRLQARCRGYLVRQEFRSRMNFLKKQIPAITCIQSQWRGYKQKKAYQDRLAYLRSHKDEVVKIQSLARMHQARKRYRDRLQYFRDHINDIIKIQAFIRANKARDDYKTLINAEDPPMVVVRKFVHLLDQSDQDFQEELDLMKMREEVITLIRSNQQLENDLNLMDIKIGLLVKNKITLQDVVSHSKKLTKKNKEQLSDMMMINKQKGGLKALSKEKREKLEAYQHLFYLLQTNPTYLAKLIFQMPQNKSTKFMDSVIFTLYNYASNQREEYLLLRLFKTALQEEIKSKVDQIQEIVTGNPTVIKMVVSFNRGARGQNALRQILAPVVKEIMDDKSLNIKTDPVDIYKSWVNQMESQTGEASKLPYDVTPEQALAHEEVKTRLDSSIRNMRAVTDKFLSAIVSSVDKIPYGMRFIAKVLKDSLHEKFPDAGEDELLKIIGNLLYYRYMNPAIVAPDAFDIIDLSAGGQLTTDQRRNLGSIAKMLQHAASNKMFLGDNAHLSIINEYLSQSYQKFRRFFQTACDVPELQDKFNVDEYSDLVTLTKPVIYISIGEIINTHTLLLDHQDAIAPEHNDPIHELLDDLGEVPTIESLIGESSGNLNDPNKEALAKTEVSLTLTNKFDVPGDENAEMDARTILLNTKRLIVDVIRFQPGETLTEILETPATSEQEAEHQRAMQRRAIRDAKTPDKMKKSKSVKEDSNLTLQEKKEKIQTGLKKLTELGTVDPKNKYQELINDIARDIRNQRRYRQRRKAELVKLQQTYAALNSKATFYGEQVDYYKSYIKTCLDNLASKGKVSKKPREMKGKKSKKISLKYTAARLHEKGVLLEIEDLQVNQFKNVIFEISPTEEVGDFEVKAKFMGVQMETFMLHYQDLLQLQYEGVAVMKLFDRAKVNVNLLIFLLNKKFYGK DIP protein P46940 original sequence version: 158 DIP protein P46940 original sequence version: 152 A52EC629596EAC33 rack1_human Receptor of activated protein C kinase 1 Small ribosomal subunit protein RACK1 HLC7 PIG21 RACK1 Cell proliferation-inducing gene 21 protein GNB2L1 Guanine nucleotide-binding protein subunit beta-2-like 1 Guanine nucleotide-binding protein subunit beta-like protein 12.3 Human lung cancer oncogene 7 protein Receptor for activated C kinase protein protein human Homo sapiens Human MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR DIP protein P63244 original sequence version: 71 257F91E369ED2044 park7-gnb2l1-9 2 12 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 1A park7-gnb2l1-1 4 13 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1B park7-gnb2l1-3 3 13 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 1C park7-gnb2l1-4 1 10 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 1D and 4 CHEBI:16240 hydrogen peroxide (causes formation of reactive oxygen species) CHEBI:641 MPP+ (1-methyl-4-phenylpyridinium) (causes formation of reactive oxygen species) gnb2l1-park7-2 5 8 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization 1E gnb2l1-park7 4 13 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q99497:p.Cys46Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C S Wed May 02 16:27:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys46ser' Tue Jun 30 17:44:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys46Ser' Q99497:p.Cys53Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain C S Wed May 02 19:50:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys53ser' Tue Jun 30 19:50:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys53Ser' Q99497:p.Cys106Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C S Wed May 02 16:55:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys106ser' Tue Jun 30 19:48:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys106Ser' physical association physical association 1F DJ-1 forms a complex with RACK1 possibly through C46 and C106 residues. I dont think the L166P mutation decreases the reaction?? Text says nowt. gnb2l1-park7-1 4 13 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2C DJ-1 expression enhanced the homodimerisation of RACK1, (whereas the dimerization of DJ-1 was not affected by RACK1). gnb2l1-gnb2l1 4 6 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2D Only a faint band is ever visible when blotting with anti-GFP. The dimerization of DJ-1 was not affected by RACK1, (whereas DJ-1 expression did enhance the homodimerisation of RACK1.)