IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A Parkinson's disease gene regulatory network identifies the signaling protein RGS2 as a modulator of LRRK2 activity and neuronal toxicity. A Parkinson's disease gene regulatory network identifies the signaling protein RGS2 as a modulator of LRRK2 activity and neuronal toxicity. Hum. Mol. Genet. (0964-6906) 2014 imex curation Dusonchet J., Li H., Guillily M., Liu M., Stafa K., Derada C., Boon J.Y., Glauser L., Mamais A., Citro A., Youmans K.L., Liu-Yesucevitz L., Schneider B.L., Aebischer P., Yue Z., Bandopadhyay R., Glicksman M.A., Moore D.J., Collins J.J., Wolozin B. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Results from Kinase, GTPase and RGS2 phosphorylation assays have not been included as LRRK2 was not further purified after IP. bwolozin@bu.edu,jcollins@bu.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Dusonchet J., Li H., Guillily M., Liu M., Stafa K., Derada C., Boon J.Y., Glauser L., Mamais A., Citro A., Youmans K.L., Liu-Yesucevitz L., Schneider B.L., Aebischer P., Yue Z., Bandopadhyay R., Glicksman M.A., Moore D.J., Collins J.J., Wolozin B. Parkinsons - Interactions investigated in the context of Parkinsons disease Figure 5C, bottom image:- It is stated that "excess amounts of free GTP compete for binding and eliminate precipitation of LRRK2 ." However according to the figure legend in the BOTTOM image (5C) there is NO WT-LRRK2-(V5) present when GTP is added (Lanes 3 and 4). However, an anti-myc antibody is apparently used to detect myc-tagged LRRK2, and the image does indeed show a decrease in binding suggesting the figure legend is incorrect. Results from Kinase, GTPase and RGS2 phosphorylation assays have not been included as LRRK2 was not further purified after IP. Fig 5C:- The authors replied on 4/8/14 said they would get back to me 'to get the definitive answer to this question..... after checking lab notebooks' - however these notebooks must have been lost/misplaced, as he has never got back to me. Please can you check bottom Fig 5C to make sure the caution I have added is not mis-placed.? Maybe the caution should be removed? The authors have stated that LRRK-V5 has a V5 Tag C-terminal and is human in origin.(as used in figure 5C) bwolozin@bu.edu,jcollins@bu.edu Accepted 2014-SEP-04 by ORCHARD Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation A Parkinson's disease gene regulatory network identifies the signaling protein RGS2 as a modulator of LRRK2 activity and neuronal toxicity. A Parkinson's disease gene regulatory network identifies the signaling protein RGS2 as a modulator of LRRK2 activity and neuronal toxicity. Hum. Mol. Genet. (0964-6906) 2014 imex curation Dusonchet J., Li H., Guillily M., Liu M., Stafa K., Derada C., Boon J.Y., Glauser L., Mamais A., Citro A., Youmans K.L., Liu-Yesucevitz L., Schneider B.L., Aebischer P., Yue Z., Bandopadhyay R., Glicksman M.A., Moore D.J., Collins J.J., Wolozin B. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Results from Kinase, GTPase and RGS2 phosphorylation assays have not been included as LRRK2 was not further purified after IP. bwolozin@bu.edu,jcollins@bu.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Dusonchet J., Li H., Guillily M., Liu M., Stafa K., Derada C., Boon J.Y., Glauser L., Mamais A., Citro A., Youmans K.L., Liu-Yesucevitz L., Schneider B.L., Aebischer P., Yue Z., Bandopadhyay R., Glicksman M.A., Moore D.J., Collins J.J., Wolozin B. Parkinsons - Interactions investigated in the context of Parkinsons disease Results from Kinase, GTPase and RGS2 phosphorylation assays have not been included as LRRK2 was not further purified after IP. bwolozin@bu.edu,jcollins@bu.edu Accepted 2014-SEP-04 by ORCHARD Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation A Parkinson's disease gene regulatory network identifies the signaling protein RGS2 as a modulator of LRRK2 activity and neuronal toxicity. A Parkinson's disease gene regulatory network identifies the signaling protein RGS2 as a modulator of LRRK2 activity and neuronal toxicity. Hum. Mol. Genet. (0964-6906) 2014 imex curation Dusonchet J., Li H., Guillily M., Liu M., Stafa K., Derada C., Boon J.Y., Glauser L., Mamais A., Citro A., Youmans K.L., Liu-Yesucevitz L., Schneider B.L., Aebischer P., Yue Z., Bandopadhyay R., Glicksman M.A., Moore D.J., Collins J.J., Wolozin B. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Results from Kinase, GTPase and RGS2 phosphorylation assays have not been included as LRRK2 was not further purified after IP. bwolozin@bu.edu,jcollins@bu.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro gtpase assay gtpase assay GTPase gtp hydrolisis gtp hydrolisis GTPase tag visualisation tag visualisation Dusonchet J., Li H., Guillily M., Liu M., Stafa K., Derada C., Boon J.Y., Glauser L., Mamais A., Citro A., Youmans K.L., Liu-Yesucevitz L., Schneider B.L., Aebischer P., Yue Z., Bandopadhyay R., Glicksman M.A., Moore D.J., Collins J.J., Wolozin B. Parkinsons - Interactions investigated in the context of Parkinsons disease In MATERIALS AND METHODS for the "GTP hydrolysis assay" it is stated that [a-33P]-GTP was used;however the image legend for Figure 5A states that a-32P-GTP was hydrolysed. Results from Kinase, GTPase and RGS2 phosphorylation assays have not been included as LRRK2 was not further purified after IP. yes - they have really used MOUSE LRRK2 transfected into 293 cells - see Ref 18. bwolozin@bu.edu,jcollins@bu.edu Accepted 2014-SEP-04 by ORCHARD Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation A Parkinson's disease gene regulatory network identifies the signaling protein RGS2 as a modulator of LRRK2 activity and neuronal toxicity. A Parkinson's disease gene regulatory network identifies the signaling protein RGS2 as a modulator of LRRK2 activity and neuronal toxicity. Hum. Mol. Genet. (0964-6906) 2014 imex curation Dusonchet J., Li H., Guillily M., Liu M., Stafa K., Derada C., Boon J.Y., Glauser L., Mamais A., Citro A., Youmans K.L., Liu-Yesucevitz L., Schneider B.L., Aebischer P., Yue Z., Bandopadhyay R., Glicksman M.A., Moore D.J., Collins J.J., Wolozin B. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Results from Kinase, GTPase and RGS2 phosphorylation assays have not been included as LRRK2 was not further purified after IP. bwolozin@bu.edu,jcollins@bu.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay tag visualisation tag visualisation Dusonchet J., Li H., Guillily M., Liu M., Stafa K., Derada C., Boon J.Y., Glauser L., Mamais A., Citro A., Youmans K.L., Liu-Yesucevitz L., Schneider B.L., Aebischer P., Yue Z., Bandopadhyay R., Glicksman M.A., Moore D.J., Collins J.J., Wolozin B. Parkinsons - Interactions investigated in the context of Parkinsons disease In MATERIALS AND METHODS for the "Protein Kinase assay" it is stated that 33P-ATP was added;however the image legend for Figure 5D states that 32P-ATP was used. The sequence for LRRKtide is stated to be "RLGRDKYKTLRQJRQ"; however this matches no sequence found anywhere else, so has been entered as Human Moesin with a sequence of RLGRDKYKTLRQIRQ. Results from Kinase, GTPase and RGS2 phosphorylation assays have not been included as LRRK2 was not further purified after IP. yes - they have really used MOUSE LRRK2 transfected into 293 cells - see Ref 18. bwolozin@bu.edu,jcollins@bu.edu Accepted 2014-SEP-04 by ORCHARD Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation A Parkinson's disease gene regulatory network identifies the signaling protein RGS2 as a modulator of LRRK2 activity and neuronal toxicity. A Parkinson's disease gene regulatory network identifies the signaling protein RGS2 as a modulator of LRRK2 activity and neuronal toxicity. Hum. Mol. Genet. (0964-6906) 2014 imex curation Dusonchet J., Li H., Guillily M., Liu M., Stafa K., Derada C., Boon J.Y., Glauser L., Mamais A., Citro A., Youmans K.L., Liu-Yesucevitz L., Schneider B.L., Aebischer P., Yue Z., Bandopadhyay R., Glicksman M.A., Moore D.J., Collins J.J., Wolozin B. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Results from Kinase, GTPase and RGS2 phosphorylation assays have not been included as LRRK2 was not further purified after IP. bwolozin@bu.edu,jcollins@bu.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Dusonchet J., Li H., Guillily M., Liu M., Stafa K., Derada C., Boon J.Y., Glauser L., Mamais A., Citro A., Youmans K.L., Liu-Yesucevitz L., Schneider B.L., Aebischer P., Yue Z., Bandopadhyay R., Glicksman M.A., Moore D.J., Collins J.J., Wolozin B. Parkinsons - Interactions investigated in the context of Parkinsons disease Results from Kinase, GTPase and RGS2 phosphorylation assays have not been included as LRRK2 was not further purified after IP. bwolozin@bu.edu,jcollins@bu.edu Accepted 2014-SEP-04 by ORCHARD Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation A Parkinson's disease gene regulatory network identifies the signaling protein RGS2 as a modulator of LRRK2 activity and neuronal toxicity. A Parkinson's disease gene regulatory network identifies the signaling protein RGS2 as a modulator of LRRK2 activity and neuronal toxicity. Hum. Mol. Genet. (0964-6906) 2014 imex curation Dusonchet J., Li H., Guillily M., Liu M., Stafa K., Derada C., Boon J.Y., Glauser L., Mamais A., Citro A., Youmans K.L., Liu-Yesucevitz L., Schneider B.L., Aebischer P., Yue Z., Bandopadhyay R., Glicksman M.A., Moore D.J., Collins J.J., Wolozin B. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Results from Kinase, GTPase and RGS2 phosphorylation assays have not been included as LRRK2 was not further purified after IP. bwolozin@bu.edu,jcollins@bu.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Dusonchet J., Li H., Guillily M., Liu M., Stafa K., Derada C., Boon J.Y., Glauser L., Mamais A., Citro A., Youmans K.L., Liu-Yesucevitz L., Schneider B.L., Aebischer P., Yue Z., Bandopadhyay R., Glicksman M.A., Moore D.J., Collins J.J., Wolozin B. Parkinsons - Interactions investigated in the context of Parkinsons disease In MATERIALS AND METHODS for the "RGS2 phosphorylation assay" it is stated that 33P-ATP was added;however the image legend for Figure 5F states that 32P-ATP was used. Results from Kinase, GTPase and RGS2 phosphorylation assays have not been included as LRRK2 was not further purified after IP. I have included Fig 5F as though there is only an IP for purification, there is a WB of the gel which does clearly identify RGS2 - and there is only one definite "unknown" band on autoradiograph which is indicated clearly. Please delete the Expt if you dont think it is clean enough to be included... bwolozin@bu.edu,jcollins@bu.edu Accepted 2014-SEP-04 by ORCHARD Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation A Parkinson's disease gene regulatory network identifies the signaling protein RGS2 as a modulator of LRRK2 activity and neuronal toxicity. A Parkinson's disease gene regulatory network identifies the signaling protein RGS2 as a modulator of LRRK2 activity and neuronal toxicity. Hum. Mol. Genet. (0964-6906) 2014 imex curation Dusonchet J., Li H., Guillily M., Liu M., Stafa K., Derada C., Boon J.Y., Glauser L., Mamais A., Citro A., Youmans K.L., Liu-Yesucevitz L., Schneider B.L., Aebischer P., Yue Z., Bandopadhyay R., Glicksman M.A., Moore D.J., Collins J.J., Wolozin B. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Results from Kinase, GTPase and RGS2 phosphorylation assays have not been included as LRRK2 was not further purified after IP. bwolozin@bu.edu,jcollins@bu.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-brain-cortex Mus musculus brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Dusonchet J., Li H., Guillily M., Liu M., Stafa K., Derada C., Boon J.Y., Glauser L., Mamais A., Citro A., Youmans K.L., Liu-Yesucevitz L., Schneider B.L., Aebischer P., Yue Z., Bandopadhyay R., Glicksman M.A., Moore D.J., Collins J.J., Wolozin B. Parkinsons - Interactions investigated in the context of Parkinsons disease Results from Kinase, GTPase and RGS2 phosphorylation assays have not been included as LRRK2 was not further purified after IP. bwolozin@bu.edu,jcollins@bu.edu Accepted 2014-SEP-04 by ORCHARD Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation A Parkinson's disease gene regulatory network identifies the signaling protein RGS2 as a modulator of LRRK2 activity and neuronal toxicity. A Parkinson's disease gene regulatory network identifies the signaling protein RGS2 as a modulator of LRRK2 activity and neuronal toxicity. Hum. Mol. Genet. (0964-6906) 2014 imex curation Dusonchet J., Li H., Guillily M., Liu M., Stafa K., Derada C., Boon J.Y., Glauser L., Mamais A., Citro A., Youmans K.L., Liu-Yesucevitz L., Schneider B.L., Aebischer P., Yue Z., Bandopadhyay R., Glicksman M.A., Moore D.J., Collins J.J., Wolozin B. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Results from Kinase, GTPase and RGS2 phosphorylation assays have not been included as LRRK2 was not further purified after IP. bwolozin@bu.edu,jcollins@bu.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-cerebral_cortical_neuron Mouse cerebral cortical neuron brain-cortex_neuron Cerebral cortical neuron confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Dusonchet J., Li H., Guillily M., Liu M., Stafa K., Derada C., Boon J.Y., Glauser L., Mamais A., Citro A., Youmans K.L., Liu-Yesucevitz L., Schneider B.L., Aebischer P., Yue Z., Bandopadhyay R., Glicksman M.A., Moore D.J., Collins J.J., Wolozin B. Parkinsons - Interactions investigated in the context of Parkinsons disease Results from Kinase, GTPase and RGS2 phosphorylation assays have not been included as LRRK2 was not further purified after IP. bwolozin@bu.edu,jcollins@bu.edu Accepted 2014-SEP-04 by ORCHARD Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation gtp guanosine triphosphate - GTP 5'-GTP GTP Guanosine 5'-triphosphate guanosine 5'-triphosphoric acid guanosine triphosphate GUANOSINE-5'-TRIPHOSPHATE H4gtp guanosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 lrrk2_human Leucine-rich repeat serine/threonine-protein kinase 2 LRRK2 PARK8 Dardarin protein protein human Homo sapiens Human MASGSCQGCEEDEETLKKLIVRLNNVQEGKQIETLVQILEDLLVFTYSERASKLFQGKNIHVPLLIVLDSYMRVASVQQVGWSLLCKLIEVCPGTMQSLMGPQDVGNDWEVLGVHQLILKMLTVHNASVNLSVIGLKTLDLLLTSGKITLLILDEESDIFMLIFDAMHSFPANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYMILLSALTNFKDEEEIVLHVLHCLHSLAIPCNNVEVLMSGNVRCYNIVVEAMKAFPMSERIQEVSCCLLHRLTLGNFFNILVLNEVHEFVVKAVQQYPENAALQISALSCLALLTETIFLNQDLEEKNENQENDDEGEEDKLFWLEACYKALTWHRKNKHVQEAACWALNNLLMYQNSLHEKIGDEDGHFPAHREVMLSMLMHSSSKEVFQASANALSTLLEQNVNFRKILLSKGIHLNVLELMQKHIHSPEVAESGCKMLNHLFEGSNTSLDIMAAVVPKILTVMKRHETSLPVQLEALRAILHFIVPGMPEESREDTEFHHKLNMVKKQCFKNDIHKLVLAALNRFIGNPGIQKCGLKVISSIVHFPDALEMLSLEGAMDSVLHTLQMYPDDQEIQCLGLSLIGYLITKKNVFIGTGHLLAKILVSSLYRFKDVAEIQTKGFQTILAILKLSASFSKLLVHHSFDLVIFHQMSSNIMEQKDQQFLNLCCKCFAKVAMDDYLKNVMLERACDQNNSIMVECLLLLGADANQAKEGSSLICQVCEKESSPKLVELLLNSGSREQDVRKALTISIGKGDSQIISLLLRRLALDVANNSICLGGFCIGKVEPSWLGPLFPDKTSNLRKQTNIASTLARMVIRYQMKSAVEEGTASGSDGNFSEDVLSKFDEWTFIPDSSMDSVFAQSDDLDSEGSEGSFLVKKKSNSISVGEFYRDAVLQRCSPNLQRHSNSLGPIFDHEDLLKRKRKILSSDDSLRSSKLQSHMRHSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVHLEHLEKLELHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDVSRNDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGNKISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAAMPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQYLPGPAHWKSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFKHIGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQSATVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMSKITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQLVVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRENQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQILTVKVEGCPKHPKGIISRRDVEKFLSKKRKFPKNYMSQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPYMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITVPSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILTTGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKENPQERPTSAQVFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASIWLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALVHLPVEKESWIVSGTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLVGTADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWGGCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAKQNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKELAEKMRRTSVE DIP protein Q5S007 original sequence version: 36 DIP protein Q5S007 original sequence version: 68 DIP protein Q5S007 original sequence version: 64 26142A0CECBBC3F4 lrrk2_mouse Leucine-rich repeat serine/threonine-protein kinase 2 Lrrk2 protein protein mouse Mus musculus Mouse MASGACQGCEEEEEEEALKKLIVRLNNVQEGKQIETLLQLLEDMLVFTYSDRASKLFEDKNFHVPLLIVLDSYMRVASVQQAGWSLLCKLIEVCPGTLQSLIGPQDIGNDWEVLGIHRLILKMLTVHHANVNLSIVGLKALDLLLDSGKLTLLILDEECDIFLLIFDAMHRYSANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYTILLSTFGSFRRDKEIVYHVLCCLHSLAVTCSNVEVLMSGNVRCYNLVVEAMKAFPTNENIQEVSCSLFQKLTLGNFFNILVLNEVHVFVVKAVRQYPENAALQISALSCLALLTETIFLNQDLEERSETQEQSEEEDSEKLFWLEPCYKALVRHRKDKHVQEAACWALNNLLMYQNSLHEKIGDEDGQFPAHREVMLSMLMHSSSKDVFQAAAHALSTLLEQNVNFRKILLAKGVYLNVLELMQKHAHAPEVAESGCKMLSHLFEGSNPSLDTMAAVVPKILTVMKAHGTSLSVQLEALRAILHFVVPGLLEESREDSQCRPNVLRKQCFRTDIHKLVLVALNRFIGNPGIQKCGLKVISSLAHLPDATETLSLQGAVDSVLHTLQMYPDDQEIQCLGLHLMGCLMTKKNFCIGTGHLLAKILASTLQRFKDVAEVQTTGLQTTLSILELSVSFSKLLVHYSFDVVIFHQMSSSVVEQKDEQFLNLCCKCFAKVAVDDELKNTMLERACDQNNSIMVECLLLLGADANQVKGATSLIYQVCEKESSPKLVELLLNGGCREQDVRKALTVSIQKGDSQVISLLLRKLALDLANNSICLGGFGIGKIDPSWLGPLFPDKSSNLRKQTNTGSVLARKVLRYQMRNTLQEGVASGSDGKFSEDALAKFGEWTFIPDSSMDSVFGQSDDLDSEGSESSFLVKRKSNSISVGEVYRDLALQRCSPNAQRHSNSLGPVFDHEDLLRRKRKILSSDESLRSSRLPSHMRQSDSSSSLASEREHITSLDLSANELKDIDALSQKCCLSSHLEHLTKLELHQNSLTSFPQQLCETLKCLIHLDLHSNKFTSFPSFVLKMPRITNLDASRNDIGPTVVLDPAMKCPSLKQLNLSYNQLSSIPENLAQVVEKLEQLLLEGNKISGICSPLSLKELKILNLSKNHIPSLPGDFLEACSKVESFSARMNFLAAMPALPSSITSLKLSQNSFTCIPEAIFSLPHLRSLDMSHNNIECLPGPAHWKSLNLRELIFSKNQISTLDFSENPHVWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDGLHLNFDFKHVGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKMKKPELGMQGATVGIDVRDWSIQIRGKRRKDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACISKITKELLNKRGFPTIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQPVVGQLIPDCYVELEKIILSERKAVPTEFPVINRKHLLQLVNEHQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKVMAQILTVKVDGCLKHPKGIISRRDVEKFLSKKKRFPKNYMMQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPFMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNRPESFLKITVPSCRKGCILLGRVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDELMKKAEEGDLLINPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEEAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLHDIWTTGSRIMEGLRFPNEFDELAIQGKLPDPVKEYGCAPWPMVEKLITKCLKENPQERPTSAQVFDILNSAELICLMRHILIPKNIIVECMVATNLNSKSATLWLGCGNTEKGQLSLFDLNTERYSYEEVADSRILCLALVHLAAEKESWVVCGTQSGALLVINVEEETKRHTLEKMTDSVTCLHCNSLAKQSKQSNFLLVGTADGNLMIFEDKAVKCKGAAPLKTLHIGDVSTPLMCLSESLNSSERHITWGGCGTKVFSFSNDFTIQKLIETKTNQLFSYAAFSDSNIIALAVDTALYIAKKNSPVVEVWDKKTEKLCELIDCVHFLKEVMVKLNKESKHQLSYSGRVKALCLQKNTALWIGTGGGHILLLDLSTRRVIRTIHNFCDSVRAMATAQLGSLKNVMLVLGYKRKSTEGIQEQKEIQSCLSIWDLNLPHEVQNLEKHIEVRTELADKMRKTSVE DIP protein Q5S006 original sequence version: 57 706E25C173E36F98 moes_human Moesin Membrane-organizing extension spike protein MSN protein protein human Homo sapiens Human MPKTISVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWFFGLQYQDTKGFSTWLKLNKKVTAQDVRKESPLLFKFRAKFYPEDVSEELIQDITQRLFFLQVKEGILNDDIYCPPETAVLLASYAVQSKYGDFNKEVHKSGYLAGDKLLPQRVLEQHKLNKDQWEERIQVWHEEHRGMLREDAVLEYLKIAQDLEMYGVNYFSIKNKKGSELWLGVDALGLNIYEQNDRLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILALCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQMERAMLENEKKKREMAEKEKEKIEREKEELMERLKQIEEQTKKAQQELEEQTRRALELEQERKRAQSEAEKLAKERQEAEEAKEALLQASRDQKKTQEQLALEMAELTARISQLEMARQKKESEAVEWQQKAQMVQEDLEKTRAELKTAMSTPHVAEPAENEQDEQDENGAEASADLRADAMAKDRSEEERTTEAEKNERVQKHLKALTSELANARDESKKTANDMIHAENMRLGRDKYKTLRQIRQGNTKQRIDEFESM DIP protein P26038 original sequence version: 168 865A6C5CB14AE586 rgs2_human Regulator of G-protein signaling 2 G0/G1 switch regulatory protein 8 RGS2 G0S8 Cell growth-inhibiting gene 31 protein GIG31 protein protein human Homo sapiens Human MQSAMFLAVQHDCRPMDKSAGSGHKSEEKREKMKRTLLKDWKTRLSYFLQNSSTPGKPKTGKKSKQQAFIKPSPEEAQLWSEAFDELLASKYGLAAFRAFLKSEFCEENIEFWLACEDFKKTKSPQKLSSKARKIYTDFIEKEAPKEINIDFQTKTLIAQNIQEATSGCFTTAQKRVYSLMENNSYPRFLESEFYQDLCKKPQITTEPHAT EFFE4AE47EF9AD8F rgs2_mouse Regulator of G-protein signaling 2 Rgs2 protein protein mouse Mus musculus Mouse MQSAMFLAVQHDCVPMDKSAGNGPKVEEKREKMKRTLLKDWKTRLSYFLQNSSAPGKPKTGKKSKQQTFIKPSPEEAQLWAEAFDELLASKYGLAAFRAFLKSEFCEENIEFWLACEDFKKTKSPQKLSSKARKIYTDFIEKEAPKEINIDFQTKSLIAQNIQEATSGCFTTAQKRVYSLMENNSYPRFLESEFYQDLCKKPQITTEPHAT mint Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. May play a role in leukemogenesis 5D6E255C2BC7E7FA lrrk2-rgs2-1 5 13 unspecified role unspecified role prey prey n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 4 A/D PD-associated mutations in LRRK2 (G2019S, R1441C and Y1699C) had no effect on the strength of the interaction. RGS2 interacts most strongly with the F2 fragment of LRRK2 containing residues 480–895. RGS2 also interacts with all other fragments of LRRK2 tested:- 1-480,895-1503 (weakly),1534-1857,1866-2139,2125-2527(weakly). rgs2-lrrk2 5 13 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 4B lrrk2-rgs2-3 7 14 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association 4C lrrk2-rgs2-4 5 13 unspecified role unspecified role prey prey c_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range rgs_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 4 E gtp-lrrk2-21 1 9 unspecified role unspecified role bait bait gamma-amino-hexyl tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role prey prey Q5S007:p.Lys1347Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A Wed May 02 15:23:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1347ala' Tue Jun 30 17:36:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys1347Ala' n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 5B/C The presence or absence of RGS2 as well as PD-associated mutations in LRRK2 (G2019S and R1441C) had no effect on the interaction. gtp-lrrk2-22 1 9 unspecified role unspecified role bait bait gamma-amino-hexyl tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role prey prey c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 5 Ci The presence or absence of RGS2 as well as PD-associated mutations in LRRK2 (G2019S and R1441C) had no effect on the interaction. lrrk2-rgs2-6 2 13 unspecified role unspecified role neutral component neutral component n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role neutral component neutral component n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Supp 4A multiple areas of co-localization were observed. lrrk2-rgs2-7 8 10 unspecified role unspecified role neutral component neutral component n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 13 unspecified role unspecified role neutral component neutral component n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Supp Figs. 4B, 6 multiple areas of co-localization were observed. gtp-lrrk2-25 3 9 enzyme target enzyme target substrate substrate neutral component neutral component p32 or p33 isotope radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 11 enzyme enzyme neutral component neutral component n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 13 stimulator stimulator neutral component neutral component ecoli Escherichia coli (strain K12) gtpase reaction gtpase reaction 5A P41220 increases activity of Q5S006 lrrk2-msn-16 4 11 enzyme enzyme neutral component neutral component n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 13 inhibitor inhibitor neutral component neutral component ecoli Escherichia coli (strain K12) 12 enzyme target enzyme target substrate substrate neutral component neutral component region_lrrktide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) phosphorylation phosphorylation reaction phosphorylation 5D P41220 decreases activity of Q5S006 P26038 phoshores lrrk2-msn-17 6 13 enzyme target enzyme target substrate substrate neutral component neutral component n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range possible _region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 10 enzyme enzyme neutral component neutral component Q5S007:p.Gly2019Ser mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain G S Wed May 02 15:29:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser' Tue Jun 30 17:05:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly2019Ser' Q5S007:p.Asp1994Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain D A Wed May 02 15:43:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1994ala' Tue Jun 30 18:56:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1994Ala' n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation 5F P41220 phosphorylated residue It is not known if Full length (aas 1-211) or aas 111-211 GST-RGS2 were used as Novus sell both. lrrk2-3 6 10 putative self putative self putative self putative self Q5S007:p.Asp1994Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain D A Wed May 02 15:09:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1994ala' Tue Jun 30 16:18:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1994Ala' n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q5S007:p.Gly2019Ser mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain G S Wed May 02 15:32:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2019ser' Tue Jun 30 19:53:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly2019Ser' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation true 5Fi Q5S007 phosphorylated residue gtp-lrrk2-26 3 9 enzyme target enzyme target substrate substrate neutral component neutral component p32 or p33 isotope radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 11 enzyme enzyme neutral component neutral component n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. gtpase reaction gtpase reaction 5A lrrk2-msn-18 4 11 enzyme enzyme neutral component neutral component n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 12 enzyme target enzyme target substrate substrate neutral component neutral component region_lrrktide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) phosphorylation phosphorylation reaction phosphorylation 5D P26038 phosphorylated residue