IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Genes Dev. (0890-9369) 2014 imex curation Dias J., Van Nguyen N., Georgiev P., Gaub A., Brettschneider J., Cusack S., Kadlec J., Akhtar A. akhtar@immunbio.mpg.de,kadlec@embl.fr 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. anti tag coip anti tag coimmunoprecipitation anti tag coip weight by comassie molecular weight estimation by coomasie staining weight by comassie Dias J., Van Nguyen N., Georgiev P., Gaub A., Brettschneider J., Cusack S., Kadlec J., Akhtar A. akhtar@immunbio.mpg.de,kadlec@embl.fr Accepted 2014-JUL-15 by MGT Genes Dev. (0890-9369) 2014 Only protein-protein interactions imex curation Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Genes Dev. (0890-9369) 2014 imex curation Dias J., Van Nguyen N., Georgiev P., Gaub A., Brettschneider J., Cusack S., Kadlec J., Akhtar A. akhtar@immunbio.mpg.de,kadlec@embl.fr 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Dias J., Van Nguyen N., Georgiev P., Gaub A., Brettschneider J., Cusack S., Kadlec J., Akhtar A. The histone H3 N-terminal peptide corresponds to an N-terminal sequence that is highly conserved in many histone proteins from many species. akhtar@immunbio.mpg.de,kadlec@embl.fr Accepted 2014-JUL-15 by MGT Genes Dev. (0890-9369) 2014 Only protein-protein interactions imex curation Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Genes Dev. (0890-9369) 2014 imex curation Dias J., Van Nguyen N., Georgiev P., Gaub A., Brettschneider J., Cusack S., Kadlec J., Akhtar A. akhtar@immunbio.mpg.de,kadlec@embl.fr 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Dias J., Van Nguyen N., Georgiev P., Gaub A., Brettschneider J., Cusack S., Kadlec J., Akhtar A. akhtar@immunbio.mpg.de,kadlec@embl.fr Accepted 2014-JUL-15 by MGT Genes Dev. (0890-9369) 2014 Only protein-protein interactions imex curation Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Genes Dev. (0890-9369) 2014 imex curation Dias J., Van Nguyen N., Georgiev P., Gaub A., Brettschneider J., Cusack S., Kadlec J., Akhtar A. akhtar@immunbio.mpg.de,kadlec@embl.fr 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Dias J., Van Nguyen N., Georgiev P., Gaub A., Brettschneider J., Cusack S., Kadlec J., Akhtar A. akhtar@immunbio.mpg.de,kadlec@embl.fr Accepted 2014-JUL-15 by MGT Genes Dev. (0890-9369) 2014 Only protein-protein interactions imex curation Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Genes Dev. (0890-9369) 2014 imex curation Dias J., Van Nguyen N., Georgiev P., Gaub A., Brettschneider J., Cusack S., Kadlec J., Akhtar A. akhtar@immunbio.mpg.de,kadlec@embl.fr 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by staining molecular weight estimation by staining weight by staining Dias J., Van Nguyen N., Georgiev P., Gaub A., Brettschneider J., Cusack S., Kadlec J., Akhtar A. akhtar@immunbio.mpg.de,kadlec@embl.fr Accepted 2014-JUL-15 by MGT Genes Dev. (0890-9369) 2014 Only protein-protein interactions imex curation Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Genes Dev. (0890-9369) 2014 imex curation Dias J., Van Nguyen N., Georgiev P., Gaub A., Brettschneider J., Cusack S., Kadlec J., Akhtar A. akhtar@immunbio.mpg.de,kadlec@embl.fr 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight silver stain molecular weight estimation by silver staining weight silver stain Dias J., Van Nguyen N., Georgiev P., Gaub A., Brettschneider J., Cusack S., Kadlec J., Akhtar A. akhtar@immunbio.mpg.de,kadlec@embl.fr Accepted 2014-JUL-15 by MGT Genes Dev. (0890-9369) 2014 Only protein-protein interactions imex curation Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Genes Dev. (0890-9369) 2014 imex curation Dias J., Van Nguyen N., Georgiev P., Gaub A., Brettschneider J., Cusack S., Kadlec J., Akhtar A. akhtar@immunbio.mpg.de,kadlec@embl.fr 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions drome-sl2 Drosophila melanogaster sl1 cells sl2 drosophila sl12 cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Dias J., Van Nguyen N., Georgiev P., Gaub A., Brettschneider J., Cusack S., Kadlec J., Akhtar A. akhtar@immunbio.mpg.de,kadlec@embl.fr Accepted 2014-JUL-15 by MGT Genes Dev. (0890-9369) 2014 Only protein-protein interactions imex curation Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Genes Dev. (0890-9369) 2014 imex curation Dias J., Van Nguyen N., Georgiev P., Gaub A., Brettschneider J., Cusack S., Kadlec J., Akhtar A. akhtar@immunbio.mpg.de,kadlec@embl.fr 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions drome-larva Drosophila melanogaster larva larva Immature form of an organism ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Dias J., Van Nguyen N., Georgiev P., Gaub A., Brettschneider J., Cusack S., Kadlec J., Akhtar A. Male larvae Histone H3 chromatin immunoprecipitation was performed as quality control experiment for the chromatin preparation (Fig. 4b); however the interactions from this experiment could not be captured as specific identification of the interaction participants was not possible. akhtar@immunbio.mpg.de,kadlec@embl.fr Accepted 2014-JUL-15 by MGT Genes Dev. (0890-9369) 2014 Only protein-protein interactions imex curation Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex. Genes Dev. (0890-9369) 2014 imex curation Dias J., Van Nguyen N., Georgiev P., Gaub A., Brettschneider J., Cusack S., Kadlec J., Akhtar A. akhtar@immunbio.mpg.de,kadlec@embl.fr 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight silver stain molecular weight estimation by silver staining weight silver stain Dias J., Van Nguyen N., Georgiev P., Gaub A., Brettschneider J., Cusack S., Kadlec J., Akhtar A. akhtar@immunbio.mpg.de,kadlec@embl.fr Accepted 2014-JUL-15 by MGT Genes Dev. (0890-9369) 2014 Only protein-protein interactions imex curation cg15011_drome_gene CG15011 gene gene gene drome Drosophila melanogaster (Fruit fly) Fruit fly patj_drome_gene Patj homolog gene gene gene drome Drosophila melanogaster (Fruit fly) Fruit fly a4v2z1_drome nsl1 wah Dmel_CG4699 Dmel\CG4699 E(nos) NSL1 Nsl1 l(3)06536 l(3)S009413 CG4699 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAPALTAEPLSPKEKLTSTASPSARSSSESGGVGCAVGLVKKPATPTPATATTRITRSSAAAASVTASPHLQQQRSSLAINNKRKSGNAKASNEPMGLSTAPTPHSTTSPNPSTDTAAASTSAVEHNNNDSNSTSAGNSTSADNSSTPKDNSTAQLLADLTINFEETISAEICLRKTLPDVSLGKEPVPPASVLAVATSSTSAGSALSLSAVDEVTKIEIDNIEERLSQLDGNATGRPEEHVAPPSVPVPVQEAPNTPTRPQAPPSAHLESPQPARTQQQSIQQMTPQSTTSSNNFLKDGAAGAVLEDQDIEEVLKALKTFDGGHVNPDTICEFFDEVWEEAAPAAPLPPAPPENVVDVPGAKPSCSGILVSGSNISQPNVIIKQEQRPWQESHAELEQQQHVISRRIEFLLRRMRKLQARAMCRHASEEVVGIMEWSARSSHKAPVPARSSTLTEQEATVLSIVSGRPSSTFWEEQNKHPLPASQMSNVIRHIATAAKHQQICHSASGSSATLAPSSSWYNSCNSSALPMKRPRKNQLDASVATGATPTAPAPGAVTSRQAAGTTGSATGSNTPRADDIVPGYDTYVTNELTHVSGLLHTELREVQNAIDSDATESSSGGESADEMVTYNNNQQLSLPITRRAVWRYSRDRAAIALRWSWLCSQLTDLEMKIRQHSDLYMDLTQSKGEIQLESTAKTSPSLAQPANGIKEEPGSDYLCSRARPLVLSEFRKRKLFQTTNMHTISKKAARPSNIKCGCQWPQVPCTLCTGRTDPTAPRDLVETMMPQNRVALLDAGYHPVLSFASDVSQSVHLEAVARQPDWQYRVMRSQPKAIVKAMWKAERETIASGGVGGQGGSRRSGDAAKRRYIRRKERNNNSNKEAGSGAKAAAVAAGGAGGNGSSSVGGGDSGNGTAARGKRQPQHPTGANSSSSLWPDSRQRQRQRHHSPSSTSISALSGAKKARKSATNSSSSNSTQQQQNHHGTKINNHHLNGYGDQWGEQSRSRRNSSPSNSHKNERTSERRVRPIYDINNIVIPYSMLAQSKMEIIPYKEIPIPKWRIVDSDNDKGKHSSDESMERKLSNGCVASKSEELAKQQLPPPPDQDQEQPALQPPKVNGLKENQAVKHNNNNNTNNNNNKNGLVNGNAKKDELKAAEDHDTQSKEDDDPKEKVVKPKLNGNLIKNPIDKTAETQDKIAQPAPEPPLPKRPKLETPTSNEVTKANGQLVQQQLESRESEEEPQHVDEDLSDEAFIMRHQRALLEERRRFETFLKFPWSTRSRANRRVDSRAESSGANTPDPASPAPHLGGPGHDNESIPSPLAYPLDAFNESGELLTGGQARQARRRTTSSKLKDQLERRSTTPDLRESYTVMPSPFEPLNFPLSDEVYQRLLAETYAPPRSMTVSKRAKSKSISSNCDGGNTFTGGSGSRRSSKCKIKPNGQLNGQLNGHPAAAKLNGIDAANEGGISEPEEEEMLLEDEDDDYPKHHLAPLDDEEPSTPDVEHELYDSAIDAYLADPEALEEDMGEDPFEDDDPNDPEWKTRTEGVRSRRI B05F8E9A6D930A10 wds_drome Protein will die slowly wds CG17437 l(1)3Ad l(1)zw8 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MVPIGAVHGGHPGVVHPPQQPLPTAPSGPNSLQPNSVGQPGATTSSNSSASNKSSLSVKPNYTLKFTLAGHTKAVSAVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSRLLVSGSDDKTLKVWELSTGKSLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVRTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNMVYIWNLQSKEVVQKLQGHTDTVLCTACHPTENIIASAALENDKTIKLWKSDT B2A64127F1E0292D q9vaf4_drome KAT8 regulatory NSL complex subunit 2 dgt1 Dgt1 Dmel\CG18041 NSL2 NSL complex protein NSL2 Non-specific lethal 2 homolog CG18041 Dmel_CG18041 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSSFSPNQLRQCARFTGTPCTPDKEHQLREQIHSELVKIKYCANPTYECSLMRIEGYEYCIRHILRDPRSNFRQCSHVYSNGRKCINAVAKYDNKKELILTTLCFEHNRQTQLQKTHMNVGRIRSRVETNETLLHSLSSHINVEDIKPEVKKIERDDDEIDVVSPHVTPFVTQDHRNSIGHVVESSRKRRRILDYASDSSSNDADPPCLRNTAQDIEFYESDHESIDSEDDDPLKHAGVFTRAEAVKIAETKLIKLQGLYREQISYLQNVLRQRRRKYLHDIRRERELYCSIHDQLKDTPHERKLYEQLKALNSYHRRQGVEAVLYKKLKEKRARDALYAAKSSSNPKCVFTEGGVKCGERTLPCCKHCRKHILEDKRQVLFRACGVERSGVVCQEPVASILEDSSCVLHLTVAPAKRQYIQKKCESETEEEETQSTSLISTIKKEEEDPSDKLYTSQALNVTTAHGEDNKGDVKPREYAPNRS 727331679E43D7A1 q9vga4_drome Dm Tam3 Dmel\CG10042 MBD MBDR2 Mbdr2 Mbrd2 NSL TAM3 dMBD-R2 mbd-R2 Dmel_CG10042 MBD-R2 CG17393 CG10042 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGRRCCVANCPSTSRLLEHNGVTYHSFPLDPIIRAIWIKNSRISLERQITKSVLVCSRHFRRLDFNTIRNGKYLLKPRVFPTVFPWGKMDTAEIEADQRALQHASVEGTTETPGNAQSSTNDDVIKATVDQIVAQILSESAERKATEEGKTGKAADDVKNTPESGEDANKAAAKEPSGTPVAASSEATVPAPGSASSSNSPLPGTPPKYSNPHNLTIGARLEALSVEGNWLPARIVEVNETEQTLLVRFERNHKLKVSPSTSGSFQEWMAIKSERLRQRLSNRVLPVFELDEKCMARWSGPRKFPGTIRKLLGNDTYEVLFDDGYVKNVRAVHMNKMPKQLPPVQVAEEGASKSPTPVGTPVSSAPVAPKRASTGSLGGSSGSSGSKKSKCAPQRRDWPLLDMASLDIASLGLPEIPHDGEWTCHWVNDQPIGTEGFLIVGEHQKPTVIVQDWRLPPGWIKHMYQRSNVLGKWDVILVSPSGKRFRSKSDLKLFLESQNLVYNPDVYDFSIHRRRAKDINAYVYTHGYNPQPPPKPRPMDVSMNSTLDQSITSQHSLPSTPMPVKESQYMEAPVASLMPPAELMSPQTQPADETKPKIEAEILEASEGGTSQLMLADPHVVENGFAFIGGLKVKIQDNLYVCPREDCAKTYRKEDFLLIHIRHYHKEFAEHVSHCPKMQELAVKRTHPSSIDQVEAVPKNQIPNQQFFAKMHQQDLQQSRSFKRQSVSATATSSTPSDITPTKALLSPKMEPPSVSPTVESGIKQEDVSLDAGPTQSFNPSLSRSCKRARLSPSKRPSGSRKSNRQRSQRRPILSDNPAGHGLTEQDVEETRQSFNTPTPDTRIDSKKRRSGAATTPISSIDSPAMGDSVSTPSSNDQTDINAALAPPPAETLSKAPQYIKENGELIRIVRMRQEEIINCICEYGEEDGLMIQCELCLCWQHGACNGIVKEADVPDKYVCYICRNPQRGRDSMRFKHDQDWLFEGKLPVAAYHTANPQATKQFELLKRSHTLTGNLLDAKRSMHSLLVKINIARNRCHPKLYLWAKKWDEDNLDSTALTPVKRAKLEAPDLPNVPQPEAAIDPEECQYRLIEHIKVQQSLVLDRLNDIEAEMDELEKEDTLDDLKDADISTTKEALATFIKELETMKRLAKLNQVANMKQTLRDSATSK 8E8BA905AB99D00C cg6506_drome_gene CG6506 gene gene gene drome Drosophila melanogaster (Fruit fly) Fruit fly q8t076_drome Dmel\CG8233 NSL3 Nsl3 cg8233 CG8233 Dmel_CG8233 Rcd1 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MKESGSVSKNQPQTSEARGSIITYSPSKFLTSVGTGRTTYSTHTHTTTATSAATTATKTNNLPMGGSSAAYYVVKAGSLGSAASMSGCIKVSSPPKGPPMAKSPALVVTSPCTPTESSSTISAPSESSFSEKMEHSYMRDTPVRSDVHNGLTPARNILVRHPPQCPSCHTYPPQHEELSEIQQAHVPPYDEVVAKEAMNECARIAKYVKNNNSDEQDWVARVNKFNWTPMQEMVFEKVCSILDQDQLARLANDKRQHEPIHRRISSDKSASRFRKLLASVAWESRITQWIHALLMEHLSPSYMASYLEILQTLKTKLPTLVDKMLFGRPLNNSQELLAPVMKKRWEPNIMPKSRQLTHNAIMVVLPNMPTTGTVSDRMQKWYQALSTITQVVQISLPNTNNRIGNQNLDHVAETIVSITRVKINELRTENPSRGIILVGFNAGAALALQVALSESVACVVCMGFAYNTIRGPRGTPDDRMLDIKAPILFVIGQNSARTSQEEMEGLRERMQSESSLVVVGSADDALRVPKSKRRIEGVTQSMVDYMVVEEIFEFVNRTLSNPPGPRMPTCLMHQQGYQRLPKQSPHILADGNANKAVQQMRKRKMDGGLDDSMGQPSKTKFVPHNDAQDYGDAQTTAGGSASLPKVITPGVLGKQLPPVNLAAGTKIKMIPSSQFVQIKSLPSQSKLINYTLNKPSVGTSSTANIGSVVKTLPCSSVSGQQIITLKTPSGQTQQFATAATSTGAAGSSTSTGTGQKKYTVFKNANGMTMLHLTKSVPTTGSSSGSANMDLSNIIDMPIVFADNEGNIPEHQQADKDIKPAPIRSANKSPLIISQKIIKEANKPSGMVTSGSITAVSKPGNIVLNKGLQQLLNSSPGGTLATQNKVVYLNRSTIKPMGNVAPGTHRVPIRIVSTPNTGGAVTTTASSSPIMVDPATGSKVAKTVNVQTIKPTGSVLAQGTIRQAGNVGTKSYLQMINTAPGASKTVAGDGKGSVRNIYFKSATGLKQLPVQMLGNRIPGGAVISSTASASSGSPGLRRVVNIGPVSKVTATSTTSSSIAATLTHNKM 72E8C9BAEA6707DD q9vzj3_drome Rcd5 Dmel\CG1135 MCRS1 MCRS2 dMCRS1 dMCRS2 l(3)r0166 l(3)rG166 p78 Dmel_CG1135 CG1135 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MEASRITAIASSAVSVTAPNPPTVSTIPTAAASTLIQVGVSPATTTMPTPAATTTTTTIGSTASSAVGISTPIRNPISNLQIEQQNDQKRRSSSRTIKRKRFDDEIVEYNIAVPTNRSGTDANRSSRPRTTSQNYPALVGVPHTTLAPLNIPTSTPQTPLSVDSLLPGTPSTVASLSLATPTTPAPLATPLPVAPIVTAVAHPKPPAMERSTTSERRSRPVRPASKKAQRRNGRPMGQMATKDLGRWKPIDDLALIIGIQQTNDLRIIHRGVKFSCKFTLQELQQRWYALLYEPAVSRIAVSAIRNLHPELVESVQRKALYSVQEEDLLGTIKSSEQPKLEQFQELLDKNASVFYCARTAKSLQNHWLLLKQYTLLPDQSVKPIYGTDQQPLSFSDAEDQIFEHDLNEPRDEALEMERALADRRNKRNIRLLENELSRWAVLVDSVLSPTAASEFDNQTLACLCGRHVRYLMRSKEITFGRDAKDCVVDVDLGLEGPAAKISRRQGTIKLRSNGDFFIANEGKRAIFIDGTPLLSANKARLGHNCTVEISGLRFTFLVNYELINAIRQESAKTSNPLN 066C075832031025 wdr5_human WD repeat-containing protein 5 BMP2-induced 3-kb gene protein WDR5 BIG3 protein protein human Homo sapiens Human MATEEKKPETEAARAQPTPSSSATQSKPTPVKPNYALKFTLAGHTKAVSSVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNLVYIWNLQTKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLWKSDC DIP protein P61964 original sequence version: 35 DIP protein P61964 original sequence version: 131 DIP protein P61964 original sequence version: 83 DIP protein P61964 original sequence version: 84 DIP protein P61964 original sequence version: 87 DIP protein P61964 original sequence version: 116 DIP protein P61964 original sequence version: 130 DIP protein P61964 original sequence version: 113 DIP protein P61964 original sequence version: 112 DIP protein P61964 original sequence version: 99 4BF30914A2250286 kanl2_human KAT8 regulatory NSL complex subunit 2 KANSL2 C12orf41 NSL complex protein NSL2 NSL2 Non-specific lethal 2 homolog protein protein human Homo sapiens Human MNRIRIHVLPTNRGRITPVPRSQEPLSCAFTHRPCSHPRLEGQEFCIKHILEDKNAPFKQCSYISTKNGKRCPNAAPKPEKKDGVSFCAEHVRRNALALHAQMKKTNPGPVGETLLCQLSSYAKTELGSQTPESSRSEASRILDEDSWSDGEQEPITVDQTWRGDPDSEADSIDSDQEDPLKHAGVYTAEEVALIMREKLIRLQSLYIDQFKRLQHLLKEKKRRYLHNRKVEHEALGSSLLTGPEGLLAKERENLKRLKCLRRYRQRYGVEALLHRQLKERRMLATDGAAQQAHTTRSSQRCLAFVDDVRCSNQSLPMTRHCLTHICQDTNQVLFKCCQGSEEVPCNKPVPVSLSEDPCCPLHFQLPPQMYKPEQVLSVPDDLEAGPMDLYLSAAELQPTESLPLEFSDDLDVVGDGMQCPPSPLLFDPSLTLEDHLVKEIAEDPVDILGQMQMAGDGCRSQGSRNSEKASAPLSQSGLATANGKPEPTSIS 738607766A98A87A h31_human Histone H3.1 H3FA H3FL H3FD H3FI H3FH H3FK H3FF H3FJ H3FB Histone H3/a Histone H3/b Histone H3/c Histone H3/d Histone H3/f Histone H3/h Histone H3/i Histone H3/j Histone H3/k Histone H3/l H3C1 HIST1H3A H3C2 HIST1H3B H3C3 H3FC HIST1H3C H3C4 HIST1H3D H3C6 HIST1H3E H3C7 HIST1H3F H3C8 HIST1H3G H3C10 HIST1H3H H3C11 HIST1H3I H3C12 HIST1H3J protein protein human Homo sapiens Human MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA DIP protein P68431 original sequence version: 78 DIP protein P68431 original sequence version: 79 DIP protein P68431 original sequence version: 76 DIP protein P68431 original sequence version: 92 DIP protein P68431 original sequence version: 87 DIP protein P68431 original sequence version: 101 DIP protein P68431 original sequence version: 130 DIP protein P68431 original sequence version: 115 9B89008EA50A0EF6 kanl1_human KAT8 regulatory NSL complex subunit 1 KANSL1 KIAA1267 MLL1/MLL complex subunit KANSL1 MSL1 homolog 1 MSL1V1 NSL complex protein NSL1 NSL1 Non-specific lethal 1 homolog CENP-36 protein protein human Homo sapiens Human MAAMAPALTDAAAEAHHIRFKLAPPSSTLSPGSAENNGNANILIAANGTKRKAIAAEDPSLDFRNNPTKEDLGKLQPLVASYLCSDVTSVPSKESLKLQGVFSKQTVLKSHPLLSQSYELRAELLGRQPVLEFSLENLRTMNTSGQTALPQAPVNGLAKKLTKSSTHSDHDNSTSLNGGKRALTSSALHGGEMGGSESGDLKGGMTNCTLPHRSLDVEHTTLYSNNSTANKSSVNSMEQPALQGSSRLSPGTDSSSNLGGVKLEGKKSPLSSILFSALDSDTRITALLRRQADIESRARRLQKRLQVVQAKQVERHIQHQLGGFLEKTLSKLPNLESLRPRSQLMLTRKAEAALRKAASETTTSEGLSNFLKSNSISEELERFTASGIANLRCSEQAFDSDVTDSSSGGESDIEEEELTRADPEQRHVPLRRRSEWKWAADRAAIVSRWNWLQAHVSDLEYRIRQQTDIYKQIRANKGLIVLGEVPPPEHTTDLFLPLSSEVKTDHGTDKLIESVSQPLENHGARIIGHISESLSTKSCGALRPVNGVINTLQPVLADHIPGDSSDAEEQLHKKQRLNLVSSSSDGTCVAARTRPVLSCKKRRLVRPNSIVPLSKKVHRNSTIRPGCDVNPSCALCGSGSINTMPPEIHYEAPLLERLSQLDSCVHPVLAFPDDVPTSLHFQSMLKSQWQNKPFDKIKPPKKLSLKHRAPMPGSLPDSARKDRHKLVSSFLTTAKLSHHQTRPDRTHRQHLDDVGAVPMVERVTAPKAERLLNPPPPVHDPNHSKMRLRDHSSERSEVLKHHTDMSSSSYLAATHHPPHSPLVRQLSTSSDSPAPASSSSQVTASTSQQPVRRRRGESSFDINNIVIPMSVAATTRVEKLQYKEILTPSWREVDLQSLKGSPDEENEEIEDLSDAAFAALHAKCEEMERARWLWTTSVPPQRRGSRSYRSSDGRTTPQLGSANPSTPQPASPDVSSSHSLSEYSHGQSPRSPISPELHSAPLTPVARDTPRHLASEDTRCSTPELGLDEQSVQPWERRTFPLAHSPQAECEDQLDAQERAARCTRRTSGSKTGRETEAAPTSPPIVPLKSRHLVAAATAQRPTHR B69D11BC522B9CAD kmt2b_human Histone-lysine N-methyltransferase 2B KMT2B HRX2 KIAA0304 MLL2 MLL4 Myeloid/lymphoid or mixed-lineage leukemia protein 4 TRX2 Trithorax homolog 2 WBP7 WW domain-binding protein 7 protein protein human Homo sapiens Human MAAAAGGGSCPGPGSARGRFPGRPRGAGGGGGRGGRGNGAERVRVALRRGGGATGPGGAEPGEDTALLRLLGLRRGLRRLRRLWAGPRVQRGRGRGRGRGWGPSRGCVPEEESSDGESDEEEFQGFHSDEDVAPSSLRSALRSQRGRAPRGRGRKHKTTPLPPPRLADVAPTPPKTPARKRGEEGTERMVQALTELLRRAQAPQAPRSRACEPSTPRRSRGRPPGRPAGPCRRKQQAVVVAEAAVTIPKPEPPPPVVPVKHQTGSWKCKEGPGPGPGTPRRGGQSSRGGRGGRGRGRGGGLPFVIKFVSRAKKVKMGQLSLGLESGQGQGQHEESWQDVPQRRVGSGQGGSPCWKKQEQKLDDEEEEKKEEEEKDKEGEEKEERAVAEEMMPAAEKEEAKLPPPPLTPPAPSPPPPLPPPSTSPPPPLCPPPPPPVSPPPLPSPPPPPAQEEQEESPPPVVPATCSRKRGRPPLTPSQRAEREAARAGPEGTSPPTPTPSTATGGPPEDSPTVAPKSTTFLKNIRQFIMPVVSARSSRVIKTPRRFMDEDPPKPPKVEVSPVLRPPITTSPPVPQEPAPVPSPPRAPTPPSTPVPLPEKRRSILREPTFRWTSLTRELPPPPPAPPPPPAPSPPPAPATSSRRPLLLRAPQFTPSEAHLKIYESVLTPPPLGAPEAPEPEPPPADDSPAEPEPRAVGRTNHLSLPRFAPVVTTPVKAEVSPHGAPALSNGPQTQAQLLQPLQALQTQLLPQALPPPQPQLQPPPSPQQMPPLEKARIAGVGSLPLSGVEEKMFSLLKRAKVQLFKIDQQQQQKVAASMPLSPGGQMEEVAGAVKQISDRGPVRSEDESVEAKRERPSGPESPVQGPRIKHVCRHAAVALGQARAMVPEDVPRLSALPLRDRQDLATEDTSSASETESVPSRSRRGKVEAAGPGGESEPTGSGGTLAHTPRRSLPSHHGKKMRMARCGHCRGCLRVQDCGSCVNCLDKPKFGGPNTKKQCCVYRKCDKIEARKMERLAKKGRTIVKTLLPWDSDESPEASPGPPGPRRGAGAGGPREEVVAHPGPEEQDSLLQRKSARRCVKQRPSYDIFEDSDDSEPGGPPAPRRRTPRENELPLPEPEEQSRPRKPTLQPVLQLKARRRLDKDALAPGPFASFPNGWTGKQKSPDGVHRVRVDFKEDCDLENVWLMGGLSVLTSVPGGPPMVCLLCASKGLHELVFCQVCCDPFHPFCLEEAERPLPQHHDTWCCRRCKFCHVCGRKGRGSKHLLECERCRHAYHPACLGPSYPTRATRKRRHWICSACVRCKSCGATPGKNWDVEWSGDYSLCPRCTQLYEKGNYCPICTRCYEDNDYESKMMQCAQCDHWVHAKCEGLSDEDYEILSGLPDSVLYTCGPCAGAAQPRWREALSGALQGGLRQVLQGLLSSKVVGPLLLCTQCGPDGKQLHPGPCGLQAVSQRFEDGHYKSVHSFMEDMVGILMRHSEEGETPDRRAGGQMKGLLLKLLESAFGWFDAHDPKYWRRSTRLPNGVLPNAVLPPSLDHVYAQWRQQEPETPESGQPPGDPSAAFQGKDPAAFSHLEDPRQCALCLKYGDADSKEAGRLLYIGQNEWTHVNCAIWSAEVFEENDGSLKNVHAAVARGRQMRCELCLKPGATVGCCLSSCLSNFHFMCARASYCIFQDDKKVFCQKHTDLLDGKEIVNPDGFDVLRRVYVDFEGINFKRKFLTGLEPDAINVLIGSIRIDSLGTLSDLSDCEGRLFPIGYQCSRLYWSTVDARRRCWYRCRILEYRPWGPREEPAHLEAAEENQTIVHSPAPSSEPPGGEDPPLDTDVLVPGAPERHSPIQNLDPPLRPDSGSAPPPAPRSFSGARIKVPNYSPSRRPLGGVSFGPLPSPGSPSSLTHHIPTVGDPDFPAPPRRSRRPSPLAPRPPPSRWASPPLKTSPQLRVPPPTSVVTALTPTSGELAPPGPAPSPPPPEDLGPDFEDMEVVSGLSAADLDFAASLLGTEPFQEEIVAAGAMGSSHGGPGDSSEEESSPTSRYIHFPVTVVSAPGLAPSATPGAPRIEQLDGVDDGTDSEAEAVQQPRGQGTPPSGPGVVRAGVLGAAGDRARPPEDLPSEIVDFVLKNLGGPGDGGAGPREESLPPAPPLANGSQPSQGLTASPADPTRTFAWLPGAPGVRVLSLGPAPEPPKPATSKIILVNKLGQVFVKMAGEGEPVPPPVKQPPLPPTISPTAPTSWTLPPGPLLGVLPVVGVVRPAPPPPPPPLTLVLSSGPASPPRQAIRVKRVSTFSGRSPPAPPPYKAPRLDEDGEASEDTPQVPGLGSGGFSRVRMKTPTVRGVLDLDRPGEPAGEESPGPLQERSPLLPLPEDGPPQVPDGPPDLLLESQWHHYSGEASSSEEEPPSPDDKENQAPKRTGPHLRFEISSEDGFSVEAESLEGAWRTLIEKVQEARGHARLRHLSFSGMSGARLLGIHHDAVIFLAEQLPGAQRCQHYKFRYHQQGEGQEEPPLNPHGAARAEVYLRKCTFDMFNFLASQHRVLPEGATCDEEEDEVQLRSTRRATSLELPMAMRFRHLKKTSKEAVGVYRSAIHGRGLFCKRNIDAGEMVIEYSGIVIRSVLTDKREKFYDGKGIGCYMFRMDDFDVVDATMHGNAARFINHSCEPNCFSRVIHVEGQKHIVIFALRRILRGEELTYDYKFPIEDASNKLPCNCGAKRCRRFLN DIP protein Q9UMN6 original sequence version: 158 DIP protein Q9UMN6 original sequence version: 110 DIP protein Q9UMN6 original sequence version: 111 DIP protein Q9UMN6 original sequence version: 143 DIP protein Q9UMN6-1 original sequence version: 169 DIP protein Q9UMN6-1 original sequence version: 127 C0615B981BBEB7BF tho2_drome_gene Tho2 gene gene gene drome Drosophila melanogaster (Fruit fly) Fruit fly mof_drome Males-absent on the first protein mof Histone acetyl transferase MOF CG3025 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSEAELEQTPSAGHVQEQPIEEEHEPEQEPTDAYTIGGPPRTPVEDAAAELSASLDVSGSDQSAEQSLDLSGVQAEAAAESEPPAKRQHRDISPISEDSTPASSTSTSSTRSSSSSRYDDVSEAEEAPPEPEPEQPQQQQQEEKKEDGQDQVKSPGPVELEAQEPAQPQKQKEVVDQEIETEDEPSSDTVICVADINPYGSGSNIDDFVMDPDAPPNAIITEVVTIPAPLHLKGTQQLGLPLAAPPPPPPPPAAEQVPETPASPTDDGEEPPAVYLSPYIRSRYMQESTPGLPTRLAPRDPRQRNMPPPAVVLPIQTVLSANVEAISDDSSETSSSDDDEEEEEDEDDALTMEHDNTSRETVITTGDPLMQKIDISENPDKIYFIRREDGTVHRGQVLQSRTTENAAAPDEYYVHYVGLNRRLDGWVGRHRISDNADDLGGITVLPAPPLAPDQPSTSREMLAQQAAAAAAASSERQKRAANKDYYLSYCENSRYDYSDRKMTRYQKRRYDEINHVQKSHAELTATQAALEKEHESITKIKYIDKLQFGNYEIDTWYFSPFPEEYGKARTLYVCEYCLKYMRFRSSYAYHLHECDRRRPPGREIYRKGNISIYEVNGKEESLYCQLLCLMAKLFLDHKVLYFDMDPFLFYILCETDKEGSHIVGYFSKEKKSLENYNVACILVLPPHQRKGFGKLLIAFSYELSRKEGVIGSPEKPLSDLGRLSYRSYWAYTLLELMKTRCAPEQITIKELSEMSGITHDDIIYTLQSMKMIKYWKGQNVICVTSKTIQDHLQLPQFKQPKLTIDTDYLVWSPQTAAAVVRAPGNSG DIP protein O02193 original sequence version: 70 8B9C5E87E3B01317 sec5_drome_gene Exocyst complex component 2 gene gene gene drome Drosophila melanogaster (Fruit fly) Fruit fly kansl1-wdr5 4 19 unspecified role unspecified role neutral component neutral component wd40 repeat sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 22 unspecified role unspecified role neutral component neutral component lig_wd40_wdr5_win_1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q7Z3B3:p.Cys599Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain C Y Wed May 02 20:22:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys599tyr' Tue Jun 30 19:28:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys599Tyr' chemical synthesis Chemical synthesis (Chemical synthesis) 31 29 direct interaction direct interaction Table 1 working 17.2%, free 18.4% 1.50A kansl1-kansl2 4 19 unspecified role unspecified role neutral component neutral component wd40 repeat sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 20 unspecified role unspecified role neutral component neutral component lig_wd40_wdr5_vdv_1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9H9L4:p.Leu405Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain L Y Wed May 02 20:00:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu405tyr' Tue Jun 30 20:19:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu405Tyr' chemical synthesis Chemical synthesis (Chemical synthesis) 22 unspecified role unspecified role neutral component neutral component lig_wd40_wdr5_win_1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q7Z3B3:p.Cys599Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain C Y Wed May 02 15:25:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys599tyr' Tue Jun 30 19:36:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys599Tyr' chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1 2.00A working 20.2%, free 22.2% nsl1-wds 4 13 unspecified role unspecified role neutral component neutral component wd40 repeat sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. NSL1 12 unspecified role unspecified role neutral component neutral component lig_wd40_wdr5_win_2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 46 44 direct interaction direct interaction Table 1 1.40A working 18.0%, free 19.0% dgt1-nsl1 4 NSL1 12 unspecified role unspecified role neutral component neutral component lig_wd40_wdr5_win_2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) NSL2 14 unspecified role unspecified role neutral component neutral component lig_wd40_wdr5_vdv_1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9VAF4:p.Glu154Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain E Y Wed May 02 19:45:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu154tyr' Tue Jun 30 18:28:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu154Tyr' chemical synthesis Chemical synthesis (Chemical synthesis) 13 unspecified role unspecified role neutral component neutral component wd40 repeat sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Table 1 2.30A working 20.8%, free 24.6% wdr5-kansl1 2 19 unspecified role unspecified role bait bait wd40 repeat sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 22 unspecified role unspecified role prey prey lig_wd40_wdr5_win_1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q7Z3B3:p.Arg592Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:51:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg592ala' Tue Jun 30 18:55:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg592Ala' Q7Z3B3:p.Cys599Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain C Y Wed May 02 18:03:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys599tyr' Tue Jun 30 18:18:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys599Tyr' chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig. 1j, Fig. 1k Temp = 25C wdr5-kansl2 2 19 unspecified role unspecified role bait bait wd40 repeat sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 20 unspecified role unspecified role prey prey lig_wd40_wdr5_vdv_1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9H9L4:p.Leu405Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain L Y Wed May 02 16:32:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu405tyr' Tue Jun 30 15:50:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu405Tyr' Q9H9L4:p.[Leu411Glu;Val413Asp] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L E certain certain sequence position certain certain certain sequence position certain V D Wed May 02 16:34:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu411glu,val413asp' Tue Jun 30 15:38:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu411Glu;Val413Asp]' chemical synthesis Chemical synthesis (Chemical synthesis) 66 64 direct interaction direct interaction Fig. 2f, Fig. 2g Temp = 25C wds-dgt1 2 13 unspecified role unspecified role bait bait wd40 repeat sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. NSL2 14 unspecified role unspecified role prey prey lig_wd40_wdr5_vdv_1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9VAF4:p.Glu154Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain E Y Wed May 02 17:33:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu154tyr' Tue Jun 30 17:28:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu154Tyr' Q9VAF4:p.[Ile160Glu;Val162Asp] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I E certain certain sequence position certain certain certain sequence position certain V D Wed May 02 15:16:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile160glu,val162asp' Tue Jun 30 18:47:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile160Glu;Val162Asp]' chemical synthesis Chemical synthesis (Chemical synthesis) 73 71 direct interaction direct interaction Fig. 2h, Fig. 2i Temp = 25C wdr5-kmt2b 2 19 unspecified role unspecified role bait bait wd40 repeat sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) MLL4 23 unspecified role unspecified role prey prey lig_wd40_wdr5_win_1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 80 78 direct interaction direct interaction Suppl. Fig. S6a Temp = 25C wdr5-kansl1-1 2 22 competitor competitor competitor prey prey lig_wd40_wdr5_win_1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q7Z3B3:p.Cys599Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain C Y Wed May 02 16:32:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys599tyr' Tue Jun 30 20:17:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys599Tyr' chemical synthesis Chemical synthesis (Chemical synthesis) MLL4 23 unspecified role unspecified role prey prey lig_wd40_wdr5_win_1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 19 unspecified role unspecified role bait bait wd40 repeat sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) physical association physical association Suppl. Fig. S6b Temp = 25C wdr5-h3-2 2 21 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P68431:p.Ser11Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain S Y Wed May 02 16:54:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser11tyr' Tue Jun 30 20:21:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser11Tyr' chemical synthesis Chemical synthesis (Chemical synthesis) 19 unspecified role unspecified role bait bait wd40 repeat sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 94 91 direct interaction direct interaction Suppl. Fig. S6c Temp = 25C wdr5-h3-3 2 19 unspecified role unspecified role bait bait wd40 repeat sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 22 competitor competitor competitor prey prey Q7Z3B3:p.Cys599Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain C Y Wed May 02 18:15:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys599tyr' Tue Jun 30 19:24:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys599Tyr' lig_wd40_wdr5_win_1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role prey prey P68431:p.Ser11Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain S Y Wed May 02 16:41:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser11tyr' Tue Jun 30 20:02:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser11Tyr' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Suppl. Fig. S6d Temp = 25C nsl1-mof 6 NSL1 12 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. 25 unspecified role unspecified role prey prey spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. physical association physical association Fig. 1a, Suppl. Fig. 1a nsl1-wds-1 6 13 unspecified role unspecified role prey prey spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. NSL1 12 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. physical association physical association Fig. 1a, Suppl. Fig. 1a nsl1-rcd5 6 NSL1 12 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. MCRS2 18 unspecified role unspecified role prey prey spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. physical association physical association Fig. 1a, Suppl. Fig. 1a nsl1-mbd_r2 6 MBD-R2 15 unspecified role unspecified role prey prey spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. NSL1 12 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. physical association physical association Fig. 1a, Suppl. Fig. 1a mbd_r2-nsl1 3 NSL1 12 unspecified role unspecified role prey prey spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. MBD-R2 15 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. physical association physical association Fig. 1b, Suppl. Fig. 1b mbd_r2-nsl1-1 3 MBD-R2 15 unspecified role unspecified role bait bait c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. NSL1 12 unspecified role unspecified role prey prey spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. physical association physical association Fig. 1b, Suppl. Fig. 1b rcd5-nsl1 3 NSL1 12 unspecified role unspecified role prey prey spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. MCRS2 18 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. physical association physical association Fig. 1b, Suppl. Fig. 1b rcd5-nsl1-1 3 NSL1 12 unspecified role unspecified role prey prey spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. MCRS2 18 unspecified role unspecified role bait bait c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. physical association physical association Fig. 1b, Suppl. Fig. 1b wds-nsl1 3 NSL1 12 unspecified role unspecified role prey prey spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. 13 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. physical association physical association Fig. 1b, Suppl. Fig. 1b wds-nsl1-1 3 13 unspecified role unspecified role bait bait c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. NSL1 12 unspecified role unspecified role prey prey spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. physical association physical association Fig. 1b, Suppl. Fig. 1b wds-nsl1-2 6 13 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. NSL1 12 unspecified role unspecified role prey prey spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. physical association physical association Fig. 1c, Suppl. Fig. 1c wds-dgt1-1 6 13 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. NSL2 14 unspecified role unspecified role prey prey spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. physical association physical association Fig. 1c, Suppl. Fig. 1c kansl1-wdr5-1 9 22 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q7Z3B3:p.Arg592Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:05:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg592ala' Tue Jun 30 19:55:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg592Ala' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 19 unspecified role unspecified role prey prey wd40 repeat sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) physical association physical association Fig. 1i rcd5-dgt1 3 MCRS2 18 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. NSL2 14 unspecified role unspecified role prey prey spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. physical association physical association Fig. 3e, Suppl. Fig. S1d rcd5-dgt1-1 3 NSL2 14 unspecified role unspecified role prey prey spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. MCRS2 18 unspecified role unspecified role bait bait c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. physical association physical association Fig. 3e, Suppl. Fig. S1d wds-dgt1-2 3 13 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. NSL2 14 unspecified role unspecified role prey prey spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. physical association physical association Fig. 3e, Suppl. Fig. S1d wds-dgt1-3 3 13 unspecified role unspecified role bait bait c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. NSL2 14 unspecified role unspecified role prey prey spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. physical association physical association Fig. 3e, Suppl. Fig. S1d wds-nsl1-3 1 13 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range NSL1 12 unspecified role unspecified role prey prey A4V2Z1:p.Arg721Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:47:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg721ala' Tue Jun 30 16:26:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg721Ala' physical association physical association Fig. 1l dgt1-mbd_r2 7 NSL1 12 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NSL3 17 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey NSL2 14 unspecified role unspecified role prey prey MCRS2 18 unspecified role unspecified role prey prey MBD-R2 15 unspecified role unspecified role prey prey 25 unspecified role unspecified role prey prey association association Fig. 3c dgt1-mbd_r2-1 7 MBD-R2 15 unspecified role unspecified role prey prey 25 unspecified role unspecified role prey prey MCRS2 18 unspecified role unspecified role prey prey NSL2 14 unspecified role unspecified role prey prey NSL1 12 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined A4V2Z1:p.Arg721Ala mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Wed May 02 17:15:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg721ala' Tue Jun 30 19:01:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg721Ala' association association Fig. 3c dgt1-mbd_r2-2 7 13 unspecified role unspecified role prey prey 25 unspecified role unspecified role prey prey MCRS2 18 unspecified role unspecified role prey prey MBD-R2 15 unspecified role unspecified role prey prey NSL3 17 unspecified role unspecified role prey prey NSL1 12 unspecified role unspecified role prey prey NSL2 14 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 3d nsl1-sec5 8 NSL1 12 unspecified role unspecified role bait bait A4V2Z1:p.Arg721Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:28:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg721ala' Tue Jun 30 18:11:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg721Ala' 26 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 4a nsl1-sec5-1 8 NSL1 12 unspecified role unspecified role bait bait A4V2Z1:p.Arg721Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:52:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg721ala' Tue Jun 30 17:16:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg721Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 26 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Suppl. Fig. S7a nsl1-cg15011 8 NSL1 12 unspecified role unspecified role bait bait A4V2Z1:p.Arg721Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:44:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg721ala' Tue Jun 30 17:42:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg721Ala' 10 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 4a nsl1-cg15011-1 8 10 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NSL1 12 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined A4V2Z1:p.Arg721Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Wed May 02 20:25:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg721ala' Tue Jun 30 17:35:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg721Ala' association association Suppl. Fig. S7a nsl1-patj 8 11 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NSL1 12 unspecified role unspecified role bait bait A4V2Z1:p.Arg721Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:36:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg721ala' Tue Jun 30 18:44:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg721Ala' association association Fig. 4a nsl1-patj-1 8 NSL1 12 unspecified role unspecified role bait bait A4V2Z1:p.Arg721Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:22:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg721ala' Tue Jun 30 16:22:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg721Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Suppl. Fig. S7a nsl1-tho2 8 24 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NSL1 12 unspecified role unspecified role bait bait A4V2Z1:p.Arg721Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:51:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg721ala' Tue Jun 30 19:24:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg721Ala' association association Fig. 4a nsl1-tho2-1 8 NSL1 12 unspecified role unspecified role bait bait A4V2Z1:p.Arg721Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:58:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg721ala' Tue Jun 30 20:21:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg721Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 24 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Suppl. Fig. S7a nsl1-cg6506 8 NSL1 12 unspecified role unspecified role bait bait A4V2Z1:p.Arg721Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Wed May 02 17:35:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg721ala' Tue Jun 30 17:14:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg721Ala' 16 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 4a nsl1-cg6506-1 8 16 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NSL1 12 unspecified role unspecified role bait bait A4V2Z1:p.Arg721Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:15:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg721ala' Tue Jun 30 18:04:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg721Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Suppl. Fig. S7a mbd_r2-sec5 8 26 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined MBD-R2 15 unspecified role unspecified role bait bait association association Fig. 4c MBD-R2 ChIP recovery was reduced in Drosophila larvae containing the arg721ala NSL1 mutant. mbd_r2-cg15011 8 10 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined MBD-R2 15 unspecified role unspecified role bait bait association association Fig. 4c MBD-R2 ChIP recovery was reduced in Drosophila larvae containing the arg721ala NSL1 mutant. mbd_r2-patj 8 MBD-R2 15 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 4c MBD-R2 ChIP recovery was reduced in Drosophila larvae containing the arg721ala NSL1 mutant. mbd_r2-tho2 8 MBD-R2 15 unspecified role unspecified role bait bait 24 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 4c MBD-R2 ChIP recovery was reduced in Drosophila larvae containing the arg721ala NSL1 mutant. mbd_r2-cg6506 8 16 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined MBD-R2 15 unspecified role unspecified role bait bait association association Fig. 4c MBD-R2 ChIP recovery was reduced in Drosophila larvae containing the arg721ala NSL1 mutant. wdr5-kansl1-2 9 22 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 19 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range wd40 repeat sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) physical association physical association Suppl. Fig. S2a kansl1-wdr5-2 5 22 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 19 unspecified role unspecified role neutral component neutral component wd40 repeat sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) physical association physical association Suppl. Fig. S2b, Suppl. Fig. S2c kansl2-wdr5 5 19 unspecified role unspecified role neutral component neutral component wd40 repeat sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 20 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9H9L4:p.Val413Asp mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V D Wed May 02 15:49:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val413asp' Tue Jun 30 16:09:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val413Asp' ecobd Escherichia coli (strain B / BL21-DE3) physical association physical association Suppl. Fig. S4, Suppl. Fig. S5a wds-nsl1-4 3 25 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. NSL1 12 unspecified role unspecified role prey prey spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. physical association physical association Fig. 1b