IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A promiscuous lipid-binding protein diversifies the subcellular sites of toll-like receptor signal transduction. A promiscuous lipid-binding protein diversifies the subcellular sites of toll-like receptor signal transduction. Cell (0092-8674) 2014 imex curation Bonham K.S., Orzalli M.H., Hayashi K., Wolf A.I., Glanemann C., Weninger W., Iwasaki A., Knipe D.M., Kagan J.C. jonathan.kagan@childrens.harvard.edu 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-bmdm Mus musculus bmdm bone marrow-derived macrophage anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bonham K.S., Orzalli M.H., Hayashi K., Wolf A.I., Glanemann C., Weninger W., Iwasaki A., Knipe D.M., Kagan J.C. jonathan.kagan@childrens.harvard.edu Accepted 2014-MAY-30 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation A promiscuous lipid-binding protein diversifies the subcellular sites of toll-like receptor signal transduction. A promiscuous lipid-binding protein diversifies the subcellular sites of toll-like receptor signal transduction. Cell (0092-8674) 2014 imex curation Bonham K.S., Orzalli M.H., Hayashi K., Wolf A.I., Glanemann C., Weninger W., Iwasaki A., Knipe D.M., Kagan J.C. jonathan.kagan@childrens.harvard.edu 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-bmdm Mus musculus bmdm bone marrow-derived macrophage pull down pull down western blot western blot Immuno blot Immuno blot Bonham K.S., Orzalli M.H., Hayashi K., Wolf A.I., Glanemann C., Weninger W., Iwasaki A., Knipe D.M., Kagan J.C. jonathan.kagan@childrens.harvard.edu Accepted 2014-MAY-30 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation A promiscuous lipid-binding protein diversifies the subcellular sites of toll-like receptor signal transduction. A promiscuous lipid-binding protein diversifies the subcellular sites of toll-like receptor signal transduction. Cell (0092-8674) 2014 imex curation Bonham K.S., Orzalli M.H., Hayashi K., Wolf A.I., Glanemann C., Weninger W., Iwasaki A., Knipe D.M., Kagan J.C. jonathan.kagan@childrens.harvard.edu 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Bonham K.S., Orzalli M.H., Hayashi K., Wolf A.I., Glanemann C., Weninger W., Iwasaki A., Knipe D.M., Kagan J.C. Details of GST-TIRAP purification adn purity taken from the referenced article, PMID: 16751103 jonathan.kagan@childrens.harvard.edu Accepted 2014-MAY-30 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation irak2_mouse Interleukin-1 receptor-associated kinase-like 2 Irak2 protein protein mouse Mus musculus Mouse MACYIYQLPSWVLDDLCRNIDTLSEWDWMQFASYVITDLTQLRKIKSMERVQGVSITRELLWWWSMRQATVQQLVDLLCHLELYRAAQIVLSWKPVPESTSPLPAFPEAVKPGAVATSRRNLKDEQEKVRPVKPRSLLDTGPIMAGAQRQRPCEMDAPCSLKTDAPDSPQSKYCSTSTSAPKQERLLGLPGDRLFWSEADVVQATEDFDQSHRISEGTFADIYQGQRNGVAFAFKKLREVAGSSPGSMDRFLQAEMQLCLRCCHANVLPLLGFCTGRQFHSLIYPYMANGSLHDRLWAQGNSDMLPWPQRASICSGLLLAVEHLHSLDIIHSNVKSANVLLDQHLNPKLAHPVAHPHPDNKKTKYTVMRTHLFQASAAYLPEHFIRVGQLTKQVDIFSCGIVLAEVLTGIPAMDKDRSPVYLKDLLLSEIPNSTSSVCSRKTSMGKAVVKEICQRHVEKRAGLLPEACEEAWATAVSVCLRRRNASVEEARVSLAGVEEQLRGQLSLPWSRVSEATGSSSNTPEETDDVDNSSLSVPSLVMMASCPGAASSPLFTGHGAAQPSTSGRQEADSSSEACTGPQTPQNATETSWKIEINEAKRRLMENIVLYKEERLDSSELFGP DIP protein Q8CFA1 original sequence version: 74 AD4692A0B6BFE733 tirap_human Toll/interleukin-1 receptor domain-containing adapter protein MyD88 adapter-like protein Adaptor protein Wyatt TIRAP MAL protein protein human Homo sapiens Human MASSTSLPAPGSRPKKPLGKMADWFRQTLLKKPKKRPNSPESTSSDASQPTSQDSPLPPSLSSVTSPSLPPTHASDSGSSRWSKDYDVCVCHSEEDLVAAQDLVSYLEGSTASLRCFLQLRDATPGGAIVSELCQALSSSHCRVLLITPGFLQDPWCKYQMLQALTEAPGAEGCTIPLLSGLSRAAYPPELRFMYYVDGRGPDGGFRQVKEAVMRYLQTLS DIP protein P58753 original sequence version: 119 44A8454D04704540 1-phosphatidyl-1d-myo-inositol 3,5-bisphosphate 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphates small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) phosphatidyl-l-serines phosphatidyl-L-serines small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) pi4p 1-phosphatidyl-1D-myo-inositol 4-phosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 1-phosphatidyl-1d-myo-inositol 4,5-bisphosphate 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphates small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) pi5p 1-phosphatidyl-1D-myo-inositol 5-phosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) myd88_mouse Myeloid differentiation primary response protein MyD88 Myd88 protein protein mouse Mus musculus Mouse MSAGDPRVGSGSLDSFMFSIPLVALNVGVRRRLSLFLNPRTPVAADWTLLAEEMGFEYLEIRELETRPDPTRSLLDAWQGRSGASVGRLLELLALLDREDILKELKSRIEEDCQKYLGKQQNQESEKPLQVARVESSVPQTKELGGITTLDDPLGQTPELFDAFICYCPNDIEFVQEMIRQLEQTDYRLKLCVSDRDVLPGTCVWSIASELIEKRCRRMVVVVSDDYLQSKECDFQTKFALSLSPGVQQKRLIPIKYKAMKKDFPSILRFITICDYTNPCTKSWFWTRLAKALSLP DIP protein P22366 original sequence version: 131 DIP protein P22366 original sequence version: 141 DIP protein P22366-1 original sequence version: 143 DIP protein P22366-1 original sequence version: 144 DIP protein P22366 original sequence version: 122 393A75C3723FFE69 1-phosphatidyl-1d-myo-inositol 3,4,5-trisphosphate 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphates small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 1-phosphatidyl-1d-myo-inositol 3,4-bisphosphate 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) irak4_mouse Interleukin-1 receptor-associated kinase 4 Irak4 protein protein mouse Mus musculus Mouse MNKPLTPSTYIRNLNVGILRKLSDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTGKSPTCELLFDWGTTNCTVGDLVDLLVQIELFAPATLLLPDAVPQTVKSLPPREAATVAQTHGPCQEKDRTSVMPMPKLEHSCEPPDSSSPDNRSVESSDTRFHSFSFHELKSITNNFDEQPASAGGNRMGEGGFGVVYKGCVNNTIVAVKKLGAMVEISTEELKQQFDQEIKVMATCQHENLVELLGFSSDSDNLCLVYAYMPNGSLLDRLSCLDGTPPLSWHTRCKVAQGTANGIRFLHENHHIHRDIKSANILLDKDFTAKISDFGLARASARLAQTVMTSRIVGTTAYMAPEALRGEITPKSDIYSFGVVLLELITGLAAVDENREPQLLLDIKEEIEDEEKTIEDYTDEKMSDADPASVEAMYSAASQCLHEKKNRRPDIAKVQQLLQEMSA DIP protein Q8R4K2 original sequence version: 107 DIP protein Q8R4K2 original sequence version: 98 DIP protein Q8R4K2 original sequence version: 77 FC11AD06983B7AEB pi3p 1-phosphatidyl-1D-myo-inositol 3-phosphates small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) myd88-irak2 1 14 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey association association Fig. 3A, 3B Lipopolysaccharide CpG DNA tirap-irak4 2 14 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Figs. 3C, 3D LPS, CpG DNA irak2-irak4 1 14 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey association association Supp. Fig. 2A Lipopolysaccharide CpG DNA myd88-irak2-4 1 14 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 5 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait association association Supp. Fig. 2B myd88-irak2-2 1 14 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 5 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait association association Supp. Fig.2B pi3p-tirap 3 5 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P58753:p.[Lys15_Lys16delinsAlaAla;Lys31_Lys32delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain KK AA certain certain sequence position certain certain certain sequence position certain KK AA Thu Jul 07 12:07:12 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was lys15_16_31_32ala Fri Jul 22 11:14:28 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'lys15ala,lys16ala,lys31ala,lys32ala' Wed May 02 19:30:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys_lys15-16ala_ala,lys_lys31-32ala_ala' Tue Jun 30 17:52:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys15_Lys16delinsAlaAla;Lys31_Lys32delinsAlaAla]' ecoli Escherichia coli (strain K12) 15 unspecified role unspecified role bait bait direct interaction direct interaction pi4p-tirap 3 5 unspecified role unspecified role prey prey P58753:p.[Lys15_Lys16delinsAlaAla;Lys31_Lys32delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain KK AA certain certain sequence position certain certain certain sequence position certain KK AA Thu Jul 07 12:07:37 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was lys15_16_31_32ala Fri Jul 22 10:56:37 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'lys15ala,lys16ala,lys31ala,lys32ala' Wed May 02 14:56:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys_lys15-16ala_ala,lys_lys31-32ala_ala' Tue Jun 30 17:40:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys15_Lys16delinsAlaAla;Lys31_Lys32delinsAlaAla]' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait direct interaction direct interaction Fig. 4A pi5p-tirap 3 5 unspecified role unspecified role prey prey P58753:p.[Lys15_Lys16delinsAlaAla;Lys31_Lys32delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain KK AA certain certain sequence position certain certain certain sequence position certain KK AA Thu Jul 07 12:07:24 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was lys15_16_31_32ala Fri Jul 22 11:13:59 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'lys15ala,lys16ala,lys31ala,lys32ala' Wed May 02 15:45:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys_lys15-16ala_ala,lys_lys31-32ala_ala' Tue Jun 30 18:29:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys15_Lys16delinsAlaAla;Lys31_Lys32delinsAlaAla]' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role bait bait direct interaction direct interaction Fig. 4A 1_phosphatidyl_1d_myo_inositol_3_4_bisphosphate-tirap 3 5 unspecified role unspecified role prey prey P58753:p.[Lys15_Lys16delinsAlaAla;Lys31_Lys32delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain KK AA certain certain sequence position certain certain certain sequence position certain KK AA Thu Jul 07 12:15:32 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was lys15_16_31_32ala Fri Jul 22 11:09:25 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'lys15ala,lys16ala,lys31ala,lys32ala' Wed May 02 20:06:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys_lys15-16ala_ala,lys_lys31-32ala_ala' Tue Jun 30 17:01:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys15_Lys16delinsAlaAla;Lys31_Lys32delinsAlaAla]' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role bait bait direct interaction direct interaction Fig. 4A 1_phosphatidyl_1d_myo_inositol_3_5_bisphosphate-tirap 3 5 unspecified role unspecified role prey prey P58753:p.[Lys15_Lys16delinsAlaAla;Lys31_Lys32delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain KK AA certain certain sequence position certain certain certain sequence position certain KK AA Thu Jul 07 12:07:38 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was lys15_16_31_32ala Fri Jul 22 10:59:38 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'lys15ala,lys16ala,lys31ala,lys32ala' Wed May 02 16:19:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys_lys15-16ala_ala,lys_lys31-32ala_ala' Tue Jun 30 17:40:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys15_Lys16delinsAlaAla;Lys31_Lys32delinsAlaAla]' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait direct interaction direct interaction Fig. 4A 1_phosphatidyl_1d_myo_inositol_3_4_5_trisphosphate-tirap 3 5 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P58753:p.[Lys15_Lys16delinsAlaAla;Lys31_Lys32delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain KK AA certain certain sequence position certain certain certain sequence position certain KK AA Thu Jul 07 12:16:26 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was lys15_16_31_32ala Fri Jul 22 10:55:12 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'lys15ala,lys16ala,lys31ala,lys32ala' Wed May 02 16:35:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys_lys15-16ala_ala,lys_lys31-32ala_ala' Tue Jun 30 17:12:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys15_Lys16delinsAlaAla;Lys31_Lys32delinsAlaAla]' ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait direct interaction direct interaction Fig. 4A 1_phosphatidyl_1d_myo_inositol_4_5_bisphosphate-tirap 3 5 unspecified role unspecified role prey prey P58753:p.[Lys15_Lys16delinsAlaAla;Lys31_Lys32delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain KK AA certain certain sequence position certain certain certain sequence position certain KK AA Thu Jul 07 12:16:20 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was lys15_16_31_32ala Fri Jul 22 11:11:54 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'lys15ala,lys16ala,lys31ala,lys32ala' Wed May 02 15:54:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys_lys15-16ala_ala,lys_lys31-32ala_ala' Tue Jun 30 18:32:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys15_Lys16delinsAlaAla;Lys31_Lys32delinsAlaAla]' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role bait bait direct interaction direct interaction Fig. 4A phosphatidyl_l_serines-tirap 3 5 unspecified role unspecified role prey prey P58753:p.[Lys15_Lys16delinsAlaAla;Lys31_Lys32delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain KK AA certain certain sequence position certain certain certain sequence position certain KK AA Thu Jul 07 12:16:43 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was lys15_16_31_32ala Fri Jul 22 11:00:01 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'lys15ala,lys16ala,lys31ala,lys32ala' Wed May 02 16:07:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys_lys15-16ala_ala,lys_lys31-32ala_ala' Tue Jun 30 17:17:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys15_Lys16delinsAlaAla;Lys31_Lys32delinsAlaAla]' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait direct interaction direct interaction Fig. 4A myd88-irak2-3 1 14 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Fig.3E, 5C TIRAP -/- cells reconstituted withwild-type TIRAP formed myddosomes in response to both lipopolysaccharide (LPS) and CpG DNA. By contrast, PLC-TIRAP was able to rescue myddosome formation solely in response to LPS, whereas PX-TIRAP and SLP2a-TIRAP were able to rescue myddosome formation in response to CpG DNA, but not LPS Lipopolysaccharide CpG DNA