IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The PRKCI and SOX2 Oncogenes Are Coamplified and Cooperate to Activate Hedgehog Signaling in Lung Squamous Cell Carcinoma. The PRKCI and SOX2 Oncogenes Are Coamplified and Cooperate to Activate Hedgehog Signaling in Lung Squamous Cell Carcinoma. Cancer Cell (1535-6108) 2014 imex curation Justilien V., Walsh M.P., Ali S.A., Thompson E.A., Murray N.R., Fields A.P. Cancer - Interactions investigated in the context of cancer fields.alan@mayo.edu 02-Apr-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Justilien V., Walsh M.P., Ali S.A., Thompson E.A., Murray N.R., Fields A.P. Cancer - Interactions investigated in the context of cancer fields.alan@mayo.edu Accepted 2014-MAR-24 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation The PRKCI and SOX2 Oncogenes Are Coamplified and Cooperate to Activate Hedgehog Signaling in Lung Squamous Cell Carcinoma. The PRKCI and SOX2 Oncogenes Are Coamplified and Cooperate to Activate Hedgehog Signaling in Lung Squamous Cell Carcinoma. Cancer Cell (1535-6108) 2014 imex curation Justilien V., Walsh M.P., Ali S.A., Thompson E.A., Murray N.R., Fields A.P. Cancer - Interactions investigated in the context of cancer fields.alan@mayo.edu 02-Apr-2014: Contacted by IntAct-Help. Only protein-protein interactions human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Justilien V., Walsh M.P., Ali S.A., Thompson E.A., Murray N.R., Fields A.P. Cancer - Interactions investigated in the context of cancer There is no cell line stated for Fig 4D - but refers to Fig 4 A/B which is oncospheres of H1299.... Cells were grown as cell spheres (oncospheres). As a result of growth conditions these cells exhibit many stem-cell like properties. (They have no relationship to the embryonic form of a tapeworm, which is also called an "oncosphere".) fields.alan@mayo.edu Accepted 2014-MAR-24 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation The PRKCI and SOX2 Oncogenes Are Coamplified and Cooperate to Activate Hedgehog Signaling in Lung Squamous Cell Carcinoma. The PRKCI and SOX2 Oncogenes Are Coamplified and Cooperate to Activate Hedgehog Signaling in Lung Squamous Cell Carcinoma. Cancer Cell (1535-6108) 2014 imex curation Justilien V., Walsh M.P., Ali S.A., Thompson E.A., Murray N.R., Fields A.P. Cancer - Interactions investigated in the context of cancer fields.alan@mayo.edu 02-Apr-2014: Contacted by IntAct-Help. Only protein-protein interactions human-h1703 Human Lung squamous cell carcinoma h1703 H1703 Lung squamous cell carcinoma http://www.lgcstandards-atcc.org/products/all/CRL-5889.aspx?gclid=CMWes4X0-rwCFekBwwodIb8A_w&geo_country=gb ATCC = CRL-5889 ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Justilien V., Walsh M.P., Ali S.A., Thompson E.A., Murray N.R., Fields A.P. Cancer - Interactions investigated in the context of cancer Cells were grown as cell spheres (oncospheres). As a result of growth conditions these cells exhibit many stem-cell like properties. (They have no relationship to the embryonic form of a tapeworm, which is also called an "oncosphere".) fields.alan@mayo.edu Accepted 2014-MAR-24 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation The PRKCI and SOX2 Oncogenes Are Coamplified and Cooperate to Activate Hedgehog Signaling in Lung Squamous Cell Carcinoma. The PRKCI and SOX2 Oncogenes Are Coamplified and Cooperate to Activate Hedgehog Signaling in Lung Squamous Cell Carcinoma. Cancer Cell (1535-6108) 2014 imex curation Justilien V., Walsh M.P., Ali S.A., Thompson E.A., Murray N.R., Fields A.P. Cancer - Interactions investigated in the context of cancer fields.alan@mayo.edu 02-Apr-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay sequence tag sequence tag identification sequence tag Justilien V., Walsh M.P., Ali S.A., Thompson E.A., Murray N.R., Fields A.P. Cancer - Interactions investigated in the context of cancer fields.alan@mayo.edu Accepted 2014-MAR-24 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation sox2_human Transcription factor SOX-2 SOX2 protein protein human Homo sapiens Human MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAHMNGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSPTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPGAEVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM DIP protein P48431 original sequence version: 127 DIP protein P48431 original sequence version: 117 EFCCAFE3E3A2B67B hhat_human_gene HHAT_HUMAN_GENE gene gene human Homo sapiens Human kpci_human Protein kinase C iota type nPKC-iota Atypical protein kinase C-lambda/iota PRKCI DXS1179E protein protein human Homo sapiens Human MPTQRDSSTMSHTVAGGGSGDHSHQVRVKAYYRGDIMITHFEPSISFEGLCNEVRDMCSFDNEQLFTMKWIDEEGDPCTVSSQLELEEAFRLYELNKDSELLIHVFPCVPERPGMPCPGEDKSIYRRGARRWRKLYCANGHTFQAKRFNRRAHCAICTDRIWGLGRQGYKCINCKLLVHKKCHKLVTIECGRHSLPQEPVMPMDQSSMHSDHAQTVIPYNPSSHESLDQVGEEKEAMNTRESGKASSSLGLQDFDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSLSVKAASVLKSFLNKDPKERLGCHPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNEPVQLTPDDDDIVRKIDQSEFEGFEYINPLLMSAEECV DIP protein P41743 original sequence version: 146 1E3F8C1D4BFC734F prkci-sox2-1 1 5 enzyme target enzyme target substrate substrate neutral component neutral component ecoli Escherichia coli (strain K12) 7 enzyme enzyme neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. phosphorylation phosphorylation reaction phosphorylation 6A P48431 phosres prkci-sox2-2 4 7 enzyme enzyme neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. 5 enzyme target enzyme target substrate substrate neutral component neutral component ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 6A P48431 phosres P48431 thr-118 O-phospho-L-threonine sox2-hhat 2 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 4D PKCi knockdown reduces binding of SOX2. SOX2 regulates HHAT promoter activity and expression by a PKCi-dependent mechanism. P41743 is required for binding of P48431 to ENSG00000054392 P48431 increases expression at mRNA level of Q5VTY9 P48431 increases expression at protein level of Q5VTY9 sox2-hhat-1 3 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Supp 4D PKCi knockdown reduces binding of SOX2. SOX2 regulates HHAT promoter activity and expression by a PKCi-dependent mechanism. P41743 is required for binding of P48431 to ENSG00000054392 P48431 increases expression at mRNA level of Q5VTY9 P48431 increases expression at protein level of Q5VTY9