IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Extracellular Architecture of the SYG-1/SYG-2 Adhesion Complex Instructs Synaptogenesis. Extracellular Architecture of the SYG-1/SYG-2 Adhesion Complex Instructs Synaptogenesis. Cell (0092-8674) 2014 imex curation Ozkan E., Chia P.H., Wang R.R., Goriatcheva N., Borek D., Otwinowski Z., Walz T., Shen K., Garcia K.C. Coordinates of constructs used for crystallisation have been taken from the PDB submission. All other constructs have been inferred from the IgG ProSite profile. kcgarcia@stanford.edu 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Ozkan E., Chia P.H., Wang R.R., Goriatcheva N., Borek D., Otwinowski Z., Walz T., Shen K., Garcia K.C. Coordinates of constructs used for crystallisation have been taken from the PDB submission. All other constructs have been inferred from the IgG ProSite profile. kcgarcia@stanford.edu Accepted 2014-MAY-12 by PPORRAS Cell (0092-8674) 2014 Only protein-protein interactions imex curation Extracellular Architecture of the SYG-1/SYG-2 Adhesion Complex Instructs Synaptogenesis. Extracellular Architecture of the SYG-1/SYG-2 Adhesion Complex Instructs Synaptogenesis. Cell (0092-8674) 2014 imex curation Ozkan E., Chia P.H., Wang R.R., Goriatcheva N., Borek D., Otwinowski Z., Walz T., Shen K., Garcia K.C. Coordinates of constructs used for crystallisation have been taken from the PDB submission. All other constructs have been inferred from the IgG ProSite profile. kcgarcia@stanford.edu 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Ozkan E., Chia P.H., Wang R.R., Goriatcheva N., Borek D., Otwinowski Z., Walz T., Shen K., Garcia K.C. Coordinates of constructs used for crystallisation have been taken from the PDB submission. All other constructs have been inferred from the IgG ProSite profile. kcgarcia@stanford.edu Accepted 2014-MAY-12 by PPORRAS Cell (0092-8674) 2014 Only protein-protein interactions imex curation Extracellular Architecture of the SYG-1/SYG-2 Adhesion Complex Instructs Synaptogenesis. Extracellular Architecture of the SYG-1/SYG-2 Adhesion Complex Instructs Synaptogenesis. Cell (0092-8674) 2014 imex curation Ozkan E., Chia P.H., Wang R.R., Goriatcheva N., Borek D., Otwinowski Z., Walz T., Shen K., Garcia K.C. Coordinates of constructs used for crystallisation have been taken from the PDB submission. All other constructs have been inferred from the IgG ProSite profile. kcgarcia@stanford.edu 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ozkan E., Chia P.H., Wang R.R., Goriatcheva N., Borek D., Otwinowski Z., Walz T., Shen K., Garcia K.C. Coordinates of constructs used for crystallisation have been taken from the PDB submission. All other constructs have been inferred from the IgG ProSite profile. kcgarcia@stanford.edu Accepted 2014-MAY-12 by PPORRAS Cell (0092-8674) 2014 Only protein-protein interactions imex curation Extracellular Architecture of the SYG-1/SYG-2 Adhesion Complex Instructs Synaptogenesis. Extracellular Architecture of the SYG-1/SYG-2 Adhesion Complex Instructs Synaptogenesis. Cell (0092-8674) 2014 imex curation Ozkan E., Chia P.H., Wang R.R., Goriatcheva N., Borek D., Otwinowski Z., Walz T., Shen K., Garcia K.C. Coordinates of constructs used for crystallisation have been taken from the PDB submission. All other constructs have been inferred from the IgG ProSite profile. kcgarcia@stanford.edu 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Ozkan E., Chia P.H., Wang R.R., Goriatcheva N., Borek D., Otwinowski Z., Walz T., Shen K., Garcia K.C. Coordinates of constructs used for crystallisation have been taken from the PDB submission. All other constructs have been inferred from the IgG ProSite profile. kcgarcia@stanford.edu Accepted 2014-MAY-12 by PPORRAS Cell (0092-8674) 2014 Only protein-protein interactions imex curation q9v787_drome hbs CG7449 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQFWITLTFAVHLATTICHVMAAVTAPTPAAQVAAPPVQKFHLTPHDLQILEGTDTLLRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGPAAGNPAIRANAKLSIVAAPSSIVIEGYARNARVEVEERQNLTLHCIAENANPAAEIVWFQGEVPVSTAPIVTVNQTAPKRFTTSSTLHLQPRAEDDYKEFSCEARHKALPPDVPMRAQVQLSVLYPPGAPFFEGYSQGETLHRGQEVQIACRSRGGNPPAQLTWYRNGVAISSPQRTSGRLSENVYKFTAAAEDNGANLVCEAKNLLATTPLRAELNLTVLYAPKDVYLSGANQAKVGDSVQLSCVTAPSNPQARISWSINGRPLDNSTYKTTSSSDGGWVSSSNISLTIDSQSRTFIAVCHALNTELTQNVVGSHTVNVLYPPSPPLLTGYNDGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAPSKLVDSKITSELSLLVNASDNNAIYKCKVQNAAIDIPLFATKTLGVHFPPETVKISVVPKNLVPGIRAKLICDSSSSNPPAKISWWKDGIPVEGLNLANRPGLWGGSVSTLEMYVNITQDLDGIVYTCQSHNEVLQRSVHETISLDILYPPKFETPQSTTFVGVEGAPFHVELLASGNPMVITYTWTKDGLPISSNSLSGQRLISDGPRLNISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITAVSEGRSFVAGEPAVLACHIRARPLEAAHVRWSRDGYDLATRTISSFENGTALLQIASVERSDIGNFTCIVDNQRGAPAAQNVLLVVQTAPEIDHSPGFTRYAARLGVRAQLICRSLASPQPSFIWRRHGKDLKMQRRNKFKSVERQVDALNFESALLIENTSPDDYGQYECVVRNSLGQASTTLEFSKPSRPDAPLQLRVGNVSDTGVDLNWTPGFDGGMQTYFRLRLKQHGEDKYKYVDAKPGHQNISLDGLKPGATYYFSVMAANEAGGSKFMPDIKLTLSKGSQPHSAEYTEKDELPNVMIIGITSAAMVLLVLNAALVAWFVIRRQNKSQSEAEPSNDDVYSKDDSQSVYKLPITALQADVQKKAAASTYLVENVDIIQSTAYPPKYQESSMCTPPYPLCNPDFTRTLPNPKRHSQRNSATGMIEGMQMRSKDDHMLISNGLYIPSPSPASSLVIKGSYISSPSPAPPADGSYFNMSDKYMSYPPVTY 77503C1A2A4A0600 syg1_caeel Synaptogenesis protein syg-1 syg-1 Synaptogenesis abnormal protein 1 K02E10.8 protein protein caeel Caenorhabditis elegans MVRWQTWPLLLLFQLVTCQQLQQRIVEAPKDTLAAVGETAILTCRVEHQQGPVQWMKDDFGLGTDRDKPLPGNKRYRMVGSAANGEYNLEISNVTLFDDDDFACQISESDHAKAVVSSKAKLTVLVRPTPPKIVKSHHSLKAIAGDPITQSCLSRKGKPPPTIGWAIASDEHGKHIVSWLGESRSKFGGIHAKPEISQETVIAHVNETTQVEEGGNNSREDSSIYSIMSNLSFIPRPEDDHKYLICISQHMTFPNKIEVDSVKLSLRYAPQINLTVASKLPLRENGSALLACNVNAKPLDNVKISWYKGNQKLRETGDTLTFETLKMEDHNRDIFCEATNEIGTTRGSIKLNVAFGARIMSTSQDKEVNEGDNAFFHCATLANPAPAIFWTRGDSDEIIGHGENLTLENVRTWQQGNYNCTATVEGFRKQILSHYLHIRGPPTVSMKDEVSASLDEATEIICEISGRPKTNNVRWTVNGKEINFNNGRITVHQYPKPYGKESILKIKDLKEEDFGVYNCSANNGLGFDNRGTLLKKRNILDWIVITAKFDRMVALAIISAGVLLVSLLCCLCMCRSNCRSRKSKFIDDQSDVTVKCEALDGQYFPEMYSSSPVDNVHLSTKDYISIPQNNPDLDFLGATGSFGPPGGLYPKCFNNSANEYIYNRYEHSYGSFGSGLSTPGGVSDMYGVAMSDKLPVMETLQEVETPKTSNYNFLSSPEVVRPISRTSTHV A1B9796DDF95727A iccr_drome Irregular chiasm C-roughest protein rst CG4125 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MLHTMQLLLLATIVGMVRSSPYTSYQNQRFAMEPQDQTAVVGARVTLPCRVINKQGTLQWTKDDFGLGTSRDLSGFERYAMVGSDEEGDYSLDIYPVMLDDDARYQCQVSPGPEGQPAIRSTFAGLTVLVPPEAPKITQGDVIYATEDRKVEIECVSVGGKPAAEITWIDGLGNVLTDNIEYTVIPLPDQRRFTAKSVLRLTPKKEHHNTNFSCQAQNTADRTYRSAKIRVEVKYAPKVKVNVMGSLPGGAGGSVGGAGGGSVHMSTGSRIVEHSQVRLECRADANPSDVRYRWFINDEPIIGGQKTEMVIRNVTRKFHDAIVKCEVQNSVGKSEDSETLDISYAPSFRQRPQSMEADVGSVVSLTCEVDSNPQPEIVWIQHPSDRVVGTSTNLTFSVSNETAGRYYCKANVPGYAEISADAYVYLKGSPAIGSQRTQYGLVGDTARIECFASSVPRARHVSWTFNGQEISSESGHDYSILVDAVPGGVKSTLIIRDSQAYHYGKYNCTVVNDYGNDVAEIQLQAKKSVSLLMTIVGGISVVAFLLVLTILVVVYIKCKKRTKLPPADVISEHQITKNGGVSCKLEPGDRTSNYSDLKVDISGGYVPYGDYSTHYSPPPQYLTTCSTKSNGSSTIMQNNHQNQLQLQQQQQQSHHQHHTQTTTLPMTFLTNSSGGSLTGSIIGSREIRQDNGLPSLQSTTASVVSSSPNGSCSNQSTTAATTTTTHVVVPSSMALSVDPRYSAIYGNPYLRSSNSSLLPPPTAV BE25D436F8D8261E kirr1_mouse Kin of IRRE-like protein 1 Kirrel1 Kin of irregular chiasm-like protein 1 Kirrel Neph1 Nephrin-like protein 1 protein protein mouse Mus musculus Mouse MTLESPSTRLMTCQSSLLPEKPRFLSQKMWAPHLVVAYLIFVTLALALPGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEETRIDGGPVILLQAGTPYNLTCRAFNAKPAATIIWFRDGTQQEGAVTSTELLKDGKRETTISQLLIEPTDLDIGRVFTCRSMNEAIPNGKETSIELDVHHPPTVTLSIEPQTVLEGERVIFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVYNKVGSTNVSTLVNVHFAPRIVVYPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQFAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGAGILVVFSFAALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASISTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPTRFDGRPSSRLSHSSGYAQLNTYSRAPASDYGTEPTPSGPSAPGGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHV 27FB31FFCCC25A2A syg2_caeel Synaptogenesis protein syg-2 syg-2 Synaptogenesis abnormal protein 2 C26G2.1 protein protein caeel Caenorhabditis elegans MLSQAILLWLFGSIHISSQFFLESPSNLSTIAGESITFRCSAEKSPEPIVYSQWKSNTGSLLGYHQEGILPGHQGRFSYIKQNAEELHLKITHVNLDDDGEYECQMLHPEEGPIRAKSFLNIIVPPQLVYFSNYQPNSIIAVKENTPLNITCVVPNVKPEPEVLWYMDGKVMSRDVKQASTPHLNKTFTVYTSLVVQSDRNDHGKVITCEAFQKETDIRITTNTTLDVLFPPSDPTVEILRNPSALRSGDNVTIACSVTGGNPPPDVFWYHENKRLQSHSTLDTRSKEIKNIYSFIASQNDNMAEYECRANNSRTGNPKRKAMKLEVNYPPASVELFGESNIRYGSSANIQCKSLPSNPASQITWIINGRSVPTPTQREFVVENGIVSSSNVSVHSNELSVEAHQINVECMATNPEGSSAKQHVIKIIAPPKAPIITGLEDRKFFEGDIVNVTCEAQGGNPLAELSWYRGSEKHLVKGRKKMKWSQVKKKLVMSLLCLCEVVIMNFFRFSKNVFYFFRFTLFIECGVNGAHNEVAGFSSYSTLALRVDRTMNTQRLKCEATNAALDEPLIESQYLSVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRATDDNKVHLGDVSLNETTRDNGFNVENVLSFTPTEEYDGTVVHCIANHPEWKHSVNTSFPLNVMYPPKMLVNDPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFDHTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPSKANMIHWYKNGEILAAQHRGHKKAILRLNATEQQSGEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKTAGILGGVAKARCKVYAVPTVEFVWEKDEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHLVNTTHTNLRLSGLATANTYFFQIRSINARGFASSWTSPVIFATLNEDGVNVAMIRNEKLKLTTWIPYILIFIVLFMLLNVVICCFVCNRTKKRKLREKTEMARTAINGGDVRQVQMYGTMMGNDGMSRRDIDDRPEMSEDEHSVRTMIEVSPNGYMQPIEPMLYESTGLLEYDYQTRSYMGSSQGTRSMTYANVPYPEPPQPSYHSNWNSLQRQSNNNTSPQHHLSTFINPNMGVRAGPINYAQLDGDLV D2E8B0A8935B4AB5 q0e9f2_drome sns 43-49 CG12495 CG13752 CG13753 CG13754 CG13755 CG18464 CG2385 CG8278 CT33230 CT33232 Dmel\CG33141 SNS Sns rost sns20 CG33141 Dmel_CG33141 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MALQQQHQRHQQHQQHQQQQQQQQQQQHIKWQQHATKRRRCRQHWIKHLQLLRLLAVIVLASAPVPSHAQQQKFRTTPHDLQVLEGAEAMMRCEVANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPARLNSAIRANAKLTVISPPASIEIKGYSHNSKVEVRENQDLQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVEESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKALSPDMPMRATVQLSVLYPPGPPYIEGYSAGETLRRGQTVELMCRSRGGNPPAQLIWYKNGSQIRMAYRTSGRLSENIYTFTAEAGDNKARFRCEASNVMSQNPLKAEVELSVLFAPTHVTVMGPTEARVGDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTIVSPEGGWTTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYMEGQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRINSVIRAADKSVSAEITILANVSDNQAQYRCEASNSATEIPLFQSTTLSVHFAPETVKIRIEPEELRPGMEATIICDSSSSNPPAKLSWWKDGIPIEGINNTSKPGLWGGTVSTLEFRVNVTQEMNGQVYTCQSANEALQRSAHEAVSLDVLYRPKFVPPPSSTAVGVEGESLQVSLQTRANPTPVTYKWTKDGTTIPQDGDHRIFADGGSLNFTRLHRDDAGIYSCSASNSQGGATLNITVVVEYGTTIKSVSENIVVNPGEDAMLSCTVEGKPLTEEHVKWERVGYDMTVKTSTTFANGTSYLHIKDAKREDVGNFRCVADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSPKPQFIWRQDKKDLPINRTYKYEVEERKIDSLTYESTLIVDKVAPADYGAYECVARNELGEAVETVRLEITSQPDPPLSLNILNVTHDTVTLAWTPGFDGGLKASYRVRYRMADREQYKYIDGLPNSHKLTIGGLRMNTLYLFSVMSWNELGQSSYLPDLARAETKEAPPPSHPASSLGGGPPTTSQTPLGGTSGMLLVGVGAGIVVVLLNVFVIGCCLHKRNEKRLKRGLELMPAELTEDSSNTPNLVIIGISLAAFGFLLVNASLVAWFFVHQRRKKVAETTNQPAKTATIEMYAPSSYNDTVTGETLSSVSEKSESYSNEGSSQPEYIDEVRKKAASTYLVEGSDMPPPRYQKDGTLPVIYPNNVVNACTLPHPRHNNGSAAIHMTRDDQMLISKGVYIPSPSPAPPPDGSYYNMNSDRYLSYPPMEYPAALDFTAQPMPMAHLQPMTVTSLATNGGPTLIGNGSVAAVAGGSGTLRRGILRGVVGVPQPDVTHHTSTVGGSPMQMLHDLHPVNLSASTLTTSTTLNGSLPTATATLPRQPHGILKDPNRNKQQQQQQQQQQQQQQLQHQQQLLNASLVGVGVPVSAPLGSLQILNLPPASGGLGNNLLMTTNAFDPTTVGLSSFGAVSQAGGIPVAYTDADGHLV D0F0D5003BA5882E q9w4t9_drome kirre CT12279 DUF Dmel\CG3653 Duf EG:163A10.1 KIRRE Kirre RH09239 duf Dmel_CG3653 CG3653 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MKRMRSSRLLVLPLILVLILTLLLQPIAVHAKSKKNKSSQSSHHGDSSSSSSSSSSSSGSSSAAASSANDESKPKGGDNGGQHFAMEPQDQTAVVGSRVTLPCRVMEKVGALQWTKDDFGLGQHRNLSGFERYSMVGSDEEGDFSLDIYPLMLDDDAKYQCQVGPGPQGEQGIRSRFAKLTVLVPPEAPKITQGDYLVTTEDREIELECVSQGGKPAAEITWIDGLGNVLTKGIEYVKEPLADSRRITARSILKLAPKKEHHNTTFTCQAQNTADRTYRSAKLLLEVKYAPKVIVSVVGGALAGGKIPEGAEVILSCQADANPHELSYRWFINDELMTGDFTTKMIIHNVSRQYHDAIVKCEVVNAVGKSEQSKKLDISFGPVFRQRPVSVEADLGATVSMRCDVAGNPEPEIEWISENSDQVVGVAAELKLKVSSETAGRYFCKAVVNGFPEIGAEATLYVKRAPIITSHKVQFGGVGGRVKIDCLAFSIPKAEHILWSFEGKIINMSSADPDIYIFEEHHLPEGVRAALIIRDSKATHFGKYNCTVMNSYGGDSLVITLLREPGNIPVLLVVMGSMFCVAIILMIVMIIIVYRKRRSRKKPMPADVIPEASRGGDKLNELKSELRSKAYDVEYSEAGGDGLAINLTQSPMPDVQMKGATLGVPLAGPVKFDERFSGDFGGDRYNRQCHIKNLKNQQETAYKGSPQANGYAHYFEYALDYSPPGGEGAAVVVGSGGVGKLKNGGMNSATLPHSAAATVNGGGAGNGGGASLPRNQRHEIQQSQQSNGFLGQPLLQNGIDSRFSAIYGNPYLRTNSSLLPPLPPPSTANPAATPAPPPYHAARHGHAHHANGGLKHFVGGAVITTSPVGNVNINGGGGGGSTPSGGGGVGVGVAAGGSVSGSSSNLTASSNTLAATPLAGGGVGNSGQCAQSPSGQFILSNNGKGHTQKGPLATHV 3E8AE918C8EF77D8 q9n9y9_drome kirre duf CG3653 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MKRMRSSRLLVLPLILVLILTLLLQPIAVHAKSKKNKSSQSSHHGDSSSSSSSSSSSSSSSGSSSAAASSANDESKPKGGDNGGQHFAMEPQDQTAVVGSRVTLPCRVMEKVGALQWTKDDFGLGQHRNLSGFERYSMVGSDEEGDFSLDIYPLMLDDDAKYQCQVGPGPQGEQGIRSRFAKLTVLVPPEAPKITQGDYLVTTEDREIELECVSQGGKPAAEITWIDGLGNVLTKGIEYVKEPLADSRRITARSILKLAPKKEHHNTTFTCQAQNTADRTYRSAKLLLEVKYAPKVIVSVVGGALAGGKIPEGAEVILSCQADANPHELSYRWFINDELMTGDFTTKMIIHNVSRQYHDAIVKCEVVNAVGKSEQSKKLDISFGPVFRQRPVSVEADLGATVSMRCDVAGNPEPEIEWISENSDQVVGVAAELKLKVSSETAGRYFCKAVVNGFPEIGAEATLYVKRAPIITSHKVQFGGVGGRVKIDCLAFSIPKAEHILWSFEGKIINMSSADPDIYIFEEHHLPEGVRAALIIRDSKATHFGKYNCTVLNSYGGDSLVITLLREPGNIPVLLVVMGSMFCVAIILMIVMIIIVYRKRRSRKKPMPADVIPEASRGGDKLNELKSELRSKAYDVEYSEAGGDGLAINLTQSPMPDVQMKGATLGVPLAGPVKFDERFSGDFGGDRYNRQCHIKNLKNQQETAYKGSPQANGYAHYFEYALDYSPPGGEGAAVVVGSGGVGKLKNGGMNSATLPHSAAATVNGGGAGNGGGASLPRNQRHEIQQSQQSNGFLGQPLLQNGIDSRFSAIYGNPYLRTNSSLLPPLPPPSTANPAATPAPPPYHAARHGHAHHANGGLKHFVGGAVITTSPVGNVNINGGGGGGSTPSGGGGVGVGVAAGGSVSGSSSNLTASSNTLAATPLAGGGVGNSGQCAQSPSGQFILSNNGKGHTQKGPLATHV DIP protein Q9N9Y9 original sequence version: 77 mint 0C4DDC392A22A197 syg_1-syg_2 3 9 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 6 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 18 16 direct interaction direct interaction Supp. Table 2 3.3A Rworking = 0.26 Rfree = 0.31 rst-1 3 7 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supp. Table 2 1.9A Rworking = 0.17 Rfree = 0.21 kirrel-1 3 8 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supp. Table 2 3.94A Rworking = 0.3 Rfree = 0.33 kirre-1 3 11 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supp. Table 2 2.3A Rworking = 0.19 Rfree = 0.24 syg_1-syg_2-1 1 9 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 6 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind range range range range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 38 35 direct interaction direct interaction Supp. Figs. 1A, 1B syg_1-syg_2-2 4 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9U3P2:p.Leu61Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A Wed May 02 18:08:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu61ala' Tue Jun 30 15:41:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu61Ala' Q9U3P2:p.Gln105Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 15:39:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln105ala' Tue Jun 30 17:49:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln105Ala' Q9U3P2:p.Gln53Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 20:26:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln53ala' Tue Jun 30 16:11:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln53Ala' Q9U3P2:p.Arg115Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 17:02:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg115ala' Tue Jun 30 18:25:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg115Ala' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 48 41 direct interaction direct interaction Supp. Figs. 1C, 1D, 3B, 3I-K, Table 1 syg_2-syg_1 4 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 6 unspecified role unspecified role prey prey B1Q236:p.Gln54Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 19:51:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln54ala' Tue Jun 30 17:37:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln54Ala' B1Q236:p.Gln105Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 19:51:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln105ala' Tue Jun 30 19:51:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln105Ala' c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind range range range range B1Q236:p.Phe60Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A Wed May 02 17:10:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe60ala' Tue Jun 30 20:14:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe60Ala' B1Q236:p.[Gln54Ala;Phe60Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Q A certain certain sequence position certain certain certain sequence position certain F A Wed May 02 16:26:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln54ala,phe60ala' Tue Jun 30 20:08:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln54Ala;Phe60Ala]' B1Q236:p.[Gln54Ala;Met56Ala;Phe60Ala;Ser107Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Q A certain certain sequence position certain certain certain sequence position certain M A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:08:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln54ala,met56ala,phe60ala,ser107ala' Tue Jun 30 17:21:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln54Ala;Met56Ala;Phe60Ala;Ser107Ala]' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 53 60 direct interaction direct interaction Fig. 2D, Supp. Figs. 1E, 3A-H, Table 1 sns-rst-1 4 7 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind range range range range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 10 unspecified role unspecified role bait bait extracellular region sufficient to bind sufficient binding region sufficient to bind range range range range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 67 69 direct interaction direct interaction Supp. Figs. 2A, 2C, 2G Table 1 hbs-rst-1 4 5 unspecified role unspecified role bait bait extracellular region sufficient to bind sufficient binding region sufficient to bind range range range range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 78 74 direct interaction direct interaction Supp. Fig. 2B, 2D, 2H, Table 1 sns-kirre-1 4 12 unspecified role unspecified role prey prey extracellular region sufficient to bind sufficient binding region sufficient to bind range range range range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role bait bait extracellular region sufficient to bind sufficient binding region sufficient to bind range range range range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 85 82 direct interaction direct interaction Supp. Figs. 2E, Table 1 hbs-kirre-2 4 12 unspecified role unspecified role prey prey extracellular region sufficient to bind sufficient binding region sufficient to bind range range range range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 5 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range extracellular region sufficient to bind sufficient binding region sufficient to bind range range range range c-terminal biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 90 94 direct interaction direct interaction Supp. Figs. 2F, Table 1 rst-rst-1 4 7 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range c-terminal biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 99 102 direct interaction direct interaction Supp. Fig. 2I, Table 1 syg_1-syg_2-3 2 6 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig. 4D, Supp. Fig. 4F