IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. Hum. Mol. Genet. (0964-6906) 2014 imex curation Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions mouse-cerebral_cortical_neuron Mouse cerebral cortical neuron brain-cortex_neuron Cerebral cortical neuron confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Accepted 2015-JUL-31 by PPORRAS Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation I have added a comment for Fig 8D- maybe this should be a "caution"??; I would have added the Kms on the sufficient to bind feature - but as it would probably not be visible on the public web site I have separated them as you suggest. (if you add the parameters on the feature it is not even visible on the Summary view...). I have altered the PTMs to the newer version of these - as a feature. The VMax values are not on any drop-down menu - and we rarely get these. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. Hum. Mol. Genet. (0964-6906) 2014 imex curation Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip sequence tag sequence tag identification sequence tag Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Accepted 2015-JUL-31 by PPORRAS Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation I have added a comment for Fig 8D- maybe this should be a "caution"??; I would have added the Kms on the sufficient to bind feature - but as it would probably not be visible on the public web site I have separated them as you suggest. (if you add the parameters on the feature it is not even visible on the Summary view...). I have altered the PTMs to the newer version of these - as a feature. The VMax values are not on any drop-down menu - and we rarely get these. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. Hum. Mol. Genet. (0964-6906) 2014 imex curation Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions mouse-cerebral_cortical_neuron Mouse cerebral cortical neuron brain-cortex_neuron Cerebral cortical neuron anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Accepted 2015-JUL-31 by PPORRAS Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation I have added a comment for Fig 8D- maybe this should be a "caution"??; I would have added the Kms on the sufficient to bind feature - but as it would probably not be visible on the public web site I have separated them as you suggest. (if you add the parameters on the feature it is not even visible on the Summary view...). I have altered the PTMs to the newer version of these - as a feature. The VMax values are not on any drop-down menu - and we rarely get these. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. Hum. Mol. Genet. (0964-6906) 2014 imex curation Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. EBI-9213734:- Y39 is NOT phosph-ed in the LUC transfected/control IP so this must be due to c-Abl =- hence I have entered here as Y39 PTM -cannot add a separate Kinase assay as purification has not occurred! The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Accepted 2015-JUL-31 by PPORRAS Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation I have added a comment for Fig 8D- maybe this should be a "caution"??; I would have added the Kms on the sufficient to bind feature - but as it would probably not be visible on the public web site I have separated them as you suggest. (if you add the parameters on the feature it is not even visible on the Summary view...). I have altered the PTMs to the newer version of these - as a feature. The VMax values are not on any drop-down menu - and we rarely get these. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. Hum. Mol. Genet. (0964-6906) 2014 imex curation Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Accepted 2015-JUL-31 by PPORRAS Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation I have added a comment for Fig 8D- maybe this should be a "caution"??; I would have added the Kms on the sufficient to bind feature - but as it would probably not be visible on the public web site I have separated them as you suggest. (if you add the parameters on the feature it is not even visible on the Summary view...). I have altered the PTMs to the newer version of these - as a feature. The VMax values are not on any drop-down menu - and we rarely get these. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. Hum. Mol. Genet. (0964-6906) 2014 imex curation Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay tag visualisation tag visualisation Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Method was found in Pluk etal.,2002, pmid:11832214. Purified Abl protein was bound to protein G-Sepharose beads. y-32P-ATP, ATP were added in buffer, and after incubation, SDS-PAGE bands from the gel underwent scintillation counting and/or phosphoimager analysis. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Accepted 2015-JUL-31 by PPORRAS Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation I have added a comment for Fig 8D- maybe this should be a "caution"??; I would have added the Kms on the sufficient to bind feature - but as it would probably not be visible on the public web site I have separated them as you suggest. (if you add the parameters on the feature it is not even visible on the Summary view...). I have altered the PTMs to the newer version of these - as a feature. The VMax values are not on any drop-down menu - and we rarely get these. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. Hum. Mol. Genet. (0964-6906) 2014 imex curation Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Accepted 2015-JUL-31 by PPORRAS Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation I have added a comment for Fig 8D- maybe this should be a "caution"??; I would have added the Kms on the sufficient to bind feature - but as it would probably not be visible on the public web site I have separated them as you suggest. (if you add the parameters on the feature it is not even visible on the Summary view...). I have altered the PTMs to the newer version of these - as a feature. The VMax values are not on any drop-down menu - and we rarely get these. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. Hum. Mol. Genet. (0964-6906) 2014 imex curation Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Accepted 2015-JUL-31 by PPORRAS Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation I have added a comment for Fig 8D- maybe this should be a "caution"??; I would have added the Kms on the sufficient to bind feature - but as it would probably not be visible on the public web site I have separated them as you suggest. (if you add the parameters on the feature it is not even visible on the Summary view...). I have altered the PTMs to the newer version of these - as a feature. The VMax values are not on any drop-down menu - and we rarely get these. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. Hum. Mol. Genet. (0964-6906) 2014 imex curation Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay fingerprinting peptide massfingerprinting fingerprinting Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Accepted 2015-JUL-31 by PPORRAS Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation I have added a comment for Fig 8D- maybe this should be a "caution"??; I would have added the Kms on the sufficient to bind feature - but as it would probably not be visible on the public web site I have separated them as you suggest. (if you add the parameters on the feature it is not even visible on the Summary view...). I have altered the PTMs to the newer version of these - as a feature. The VMax values are not on any drop-down menu - and we rarely get these. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. c-Abl phosphorylates alpha-synuclein and regulates its degradation, implication for alpha-synuclein clearance and contribution to the pathogenesis of Parkinson's Disease. Hum. Mol. Genet. (0964-6906) 2014 imex curation Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch 17-Sep-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Mahul-Mellier A.L., Fauvet B., Gysbers A., Dikiy I., Oueslati A., Georgeon S., Lamontanara A.J., Bisquertt A., Eliezer D., Masliah E., Halliday G., Hantschel O., Lashuel H.A. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. alpha-Syn was purified from E.coli grown in isotopically supplemented medium. "Real-Time NMR":- Sequential 1 h 1H–15N HSQC spectra were collected overnight and the relative intensity of the peaks arising from unphosphorylated and phosphorylated protein was calculated and plotted for well-resolved resonances corresponding to residues near to Y39 (Leu38 and Gly41). -the appearance of new cross-peaks for residues L38 and G41 (among others) reports on the phosphorylation at Y39. The authors have confirmed that the origin of the (Mammalian expression vectors) WT c-Abl, c-Abl K290R, and the PP c-Abl; P242E/P249E constructs, (donated by Prof. Oliver Hantschel) are Human in origin. hilal.lashuel@epfl.ch Accepted 2015-JUL-31 by PPORRAS Hum. Mol. Genet. (0964-6906) 2014 Only protein-protein interactions imex curation I have added a comment for Fig 8D- maybe this should be a "caution"??; I would have added the Kms on the sufficient to bind feature - but as it would probably not be visible on the public web site I have separated them as you suggest. (if you add the parameters on the feature it is not even visible on the Summary view...). I have altered the PTMs to the newer version of these - as a feature. The VMax values are not on any drop-down menu - and we rarely get these. abl1_mouse Tyrosine-protein kinase ABL1 Abl1 Abl Abelson murine leukemia viral oncogene homolog 1 Proto-oncogene c-Abl p150 Abelson tyrosine-protein kinase 1 protein protein mouse Mus musculus Mouse MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTIYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVSAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKRGTRGGAGSMLQAPELPTKTRTCRRAAEQKDAPDTPELLHTKGLGESDALDSEPAVSPLLPRKERGPPDGSLNEDERLLPRDRKTNLFSALIKKKKKMAPTPPKRSSSFREMDGQPDRRGASEDDSRELCNGPPALTSDAAEPTKSPKASNGAGVPNGAFREPGNSGFRSPHMWKKSSTLTGSRLAAAEEESGMSSSKRFLRSCSASCMPHGARDTEWRSVTLPRDLPSAGKQFDSSTFGGHKSEKPALPRKRTSESRSEQVAKSTAMPPPRLVKKNEEAAEEGFKDTESSPGSSPPSLTPKLLRRQVTASPSSGLSHKEEATKGSASGMGTPATAEPAPPSNKVGLSKASSEEMRVRRHKHSSESPGRDKGRLAKLKPAPPPPPACTGKAGKPAQSPSQEAGEAGGPTKTKCTSLAMDAVNTDPTKAGPPGEGLRKPVPPSVPKPQSTAKPPGTPTSPVSTPSTAPAPSPLAGDQQPSSAAFIPLISTRVSLRKTRQPPERIASGTITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLESNLRELQICPATASSGPAATQDFSKLLSSVKEISDIVRR BD48ADE8557AE95C p00519-2 Tyrosine-protein kinase ABL1 ABL1 ABL JTK7 Abelson murine leukemia viral oncogene homolog 1 Abelson tyrosine-protein kinase 1 Proto-oncogene c-Abl p150 protein protein human Homo sapiens Human MGQQPGKVLGDQRRPSLPALHFIKGAGKKESSRHGGPHCNVFVEHEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAPELPTKTRTSRRAAEHRDTTDVPEMPHSKGQGESDPLDHEPAVSPLLPRKERGPPEGGLNEDERLLPKDKKTNLFSALIKKKKKTAPTPPKRSSSFREMDGQPERRGAGEEEGRDISNGALAFTPLDTADPAKSPKPSNGAGVPNGALRESGGSGFRSPHLWKKSSTLTSSRLATGEEEGGGSSSKRFLRSCSASCVPHGAKDTEWRSVTLPRDLQSTGRQFDSSTFGGHKSEKPALPRKRAGENRSDQVTRGTVTPPPRLVKKNEEAADEVFKDIMESSPGSSPPNLTPKPLRRQVTVAPASGLPHKEEAGKGSALGTPAAAEPVTPTSKAGSGAPGGTSKGPAEESRVRRHKHSSESPGRDKGKLSRLKPAPPPPPAASAGKAGGKPSQSPSQEAAGEAVLGAKTKATSLVDAVNSDAAKPSQPGEGLKKPVLPATPKPQSAKPSGTPISPAPVPSTLPSASSALAGDQPSSTAFIPLISTRVSLRKTRQPPERIASGAITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLENNLRELQICPATAGSGPAATQDFSKLLSSVKEISDIVQR DIP protein P00519-2 original sequence version: 160 Contains a N-myristoyl glycine at position 2. DF9D4512F78FFE52 syua_human Alpha-synuclein Non-A beta component of AD amyloid Non-A4 component of amyloid precursor SNCA NACP PARK1 protein protein human Homo sapiens Human MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA DIP protein P37840 original sequence version: 124 DIP protein P37840 original sequence version: 175 DIP protein P37840 original sequence version: 178 6BB2F12128931663 crkl_human Crk-like protein CRKL protein protein human Homo sapiens Human MSSARFDSSDRSAWYMGPVSRQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSENSRVSHYIINSLPNRRFKIGDQEFDHLPALLEFYKIHYLDTTTLIEPAPRYPSPPMGSVSAPNLPTAEDNLEYVRTLYDFPGNDAEDLPFKKGEILVIIEKPEEQWWSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVNGRKGLFPFTHVKIFDPQNPDENE DIP protein P46109 original sequence version: 64 DIP protein P46109 original sequence version: 103 DIP protein P46109 original sequence version: 104 DIP protein P46109 original sequence version: 156 294CF1EE2CD44B81 syua_mouse Alpha-synuclein Snca Syn Non-A beta component of AD amyloid Non-A4 component of amyloid precursor protein protein mouse Mus musculus Mouse MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVTTVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGFVKKDQMGKGEEGYPQEGILEDMPVDPGSEAYEMPSEEGYQDYEPEA 1FFD19D7E15E636C p00519-1 Tyrosine-protein kinase ABL1 ABL1 ABL JTK7 Abelson murine leukemia viral oncogene homolog 1 Abelson tyrosine-protein kinase 1 Proto-oncogene c-Abl p150 protein protein human Homo sapiens Human MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAPELPTKTRTSRRAAEHRDTTDVPEMPHSKGQGESDPLDHEPAVSPLLPRKERGPPEGGLNEDERLLPKDKKTNLFSALIKKKKKTAPTPPKRSSSFREMDGQPERRGAGEEEGRDISNGALAFTPLDTADPAKSPKPSNGAGVPNGALRESGGSGFRSPHLWKKSSTLTSSRLATGEEEGGGSSSKRFLRSCSASCVPHGAKDTEWRSVTLPRDLQSTGRQFDSSTFGGHKSEKPALPRKRAGENRSDQVTRGTVTPPPRLVKKNEEAADEVFKDIMESSPGSSPPNLTPKPLRRQVTVAPASGLPHKEEAGKGSALGTPAAAEPVTPTSKAGSGAPGGTSKGPAEESRVRRHKHSSESPGRDKGKLSRLKPAPPPPPAASAGKAGGKPSQSPSQEAAGEAVLGAKTKATSLVDAVNSDAAKPSQPGEGLKKPVLPATPKPQSAKPSGTPISPAPVPSTLPSASSALAGDQPSSTAFIPLISTRVSLRKTRQPPERIASGAITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLENNLRELQICPATAGSGPAATQDFSKLLSSVKEISDIVQR 85FE6C1C0E483EA2 p46108-1 Adapter molecule crk Proto-oncogene c-Crk CRK p38 protein protein human Homo sapiens Human MAGNFDSEERSSWYWGRLSRQEAVALLQGQRHGVFLVRDSSTSPGDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPALLEFYKIHYLDTTTLIEPVSRSRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDILRIRDKPEEQWWNAEDSEGKRGMIPVPYVEKYRPASASVSALIGGNQEGSHPQPLGGPEPGPYAQPSVNTPLPNLQNGPIYARVIQKRVPNAYDKTALALEVGELVKVTKINVSGQWEGECNGKRGHFPFTHVRLLDQQNPDEDFS DIP protein P46108-1 original sequence version: 73 DIP protein P46108 original sequence version: 122 DIP protein P46108-1 original sequence version: 171 DIP protein P46108 original sequence version: 171 4CFBFB65BFC2E265 abl1-snca-13 9 13 enzyme target enzyme target substrate substrate unspecified role unspecified role P37840:p.[Tyr39Phe;Tyr125Phe] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 14:54:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr39phe,tyr125phe' Tue Jun 30 17:40:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr39Phe;Tyr125Phe]' P37840:p.Tyr39Phe mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 19:28:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr39phe' Tue Jun 30 17:10:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr39Phe' ecoli Escherichia coli (strain K12) 16 enzyme enzyme unspecified role unspecified role sh2_+_catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 1A P37840 O-phospho-L-tyrosine abl1-snca-1 7 13 enzyme target enzyme target substrate substrate unspecified role unspecified role P37840:p.[Tyr39Phe;Tyr125Phe] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 18:57:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr39phe,tyr125phe' Tue Jun 30 15:49:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr39Phe;Tyr125Phe]' P37840:p.Tyr39Phe mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 14:47:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr39phe' Tue Jun 30 16:20:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr39Phe' undefined mutation mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:45:20 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'tyr125phe' ecoli Escherichia coli (strain K12) 16 enzyme enzyme unspecified role unspecified role sh2_+_catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 1B P37840 tyr-39 O-phospho-L-tyrosine P37840 tyr-125 O-phospho-L-tyrosine abl1-snca-2 6 16 enzyme enzyme unspecified role unspecified role sh2_+_catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range tyr-39 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm chemical synthesis Chemical synthesis (Chemical synthesis) phosphorylation phosphorylation reaction phosphorylation 1C Y39 peptide Vmax = 13 pmol/min/ug abl1-snca-3 6 14 enzyme target enzyme target substrate substrate unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 16 enzyme enzyme unspecified role unspecified role sh2_+_catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 1C-2 P46109 tyr-207 O-phospho-L-tyrosine Y207 peptide Vmax = 36 pmol/min/ug positive control interaction abl1-snca-4 6 13 enzyme target enzyme target substrate substrate unspecified role unspecified role P37840:p.Tyr39Phe mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 18:21:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr39phe' Tue Jun 30 20:25:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr39Phe' ecoli Escherichia coli (strain K12) 16 enzyme enzyme unspecified role unspecified role sh2_+_catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 1D, suppl fig. 2A P37840 ?-? O-phospho-L-tyrosine abl1-snca-5 10 16 enzyme enzyme unspecified role unspecified role sh2_+_catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate unspecified role unspecified role region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 1E/F, Supp 2B. P37840 tyr-39 O-phospho-L-tyrosine abl1-snca-6 1 15 unspecified role unspecified role neutral component neutral component possible_phospho_39tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain possible_phospho_125tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role neutral component neutral component P00519-2:p.Lys290Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K R Wed May 02 16:12:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys290arg' Tue Jun 30 18:21:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys290Arg' colocalization colocalization 5B, Supp3B. abl1-snca-7 8 13 unspecified role unspecified role neutral component neutral component possible_phospho_39tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain possible_phospho_125tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role neutral component neutral component colocalization colocalization 6C snca-abl1-1 3 11 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait physical association physical association 8A abl1-snca-8 4 13 unspecified role unspecified role bait bait possible_phospho_39tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain possible_phospho_125tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role prey prey physical association physical association When c-Abl KD ( lys290arg mutation) was transfected rather than WT c-Abl, a band is clearly visible when an anti-c-Abl antibody is used for detection;however the c-Abl detected is not phosphorylated at Y39 or Y125. However the text stated that "co-immunoprecipitations of alpha-syn with the c-Abl kinase-dead mutant (KD c-Abl) revealed a lack of interaction between the two proteins". 8B/F/G a-synuclein was phosphorylated at Y39 only subsequent to c-Abl transfection. Y125 was already phosphorylated in control non-c-Abl transfected samples, wheras Y39 was not. Mutating Y39, Y125 or both residues to phenylalanine in a-syn did not affect its interaction with c-Abl but only its phosphorylation status. abl1-snca-9 2 12 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait possible_phospho_39tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain physical association physical association 8C/D abl1-snca-10 4 13 unspecified role unspecified role prey prey possible_phospho_39tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain possible_phospho_125tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role bait bait physical association physical association 8E abl1-snca-11 6 17 enzyme target enzyme target substrate substrate unspecified role unspecified role ecoli Escherichia coli (strain K12) 16 enzyme enzyme unspecified role unspecified role sh2_+_catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Supp 2A p46108-1 O-phospho-L-tyrosine I assume that the HIs tag has been removed as no other tag is mentioned wrt TEV cleavage. abl1-snca-12 5 12 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component possible_phospho_125tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain possible_phospho_39tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain colocalization colocalization Supp 4C abl1-snca-14 6 16 enzyme enzyme unspecified role unspecified role sh2_+_catalytic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate unspecified role unspecified role n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tyr-125 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm chemical synthesis Chemical synthesis (Chemical synthesis) phosphorylation phosphorylation reaction phosphorylation 1Ci Y125 peptide Vmax = 21pmol/min/ug