IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Declining NAD(+) Induces a Pseudohypoxic State Disrupting Nuclear-Mitochondrial Communication during Aging. Declining NAD(+) Induces a Pseudohypoxic State Disrupting Nuclear-Mitochondrial Communication during Aging. Cell (0092-8674) 2013 imex curation Gomes A.P., Price N.L., Ling A.J., Moslehi J.J., Montgomery M.K., Rajman L., White J.P., Teodoro J.S., Wrann C.D., Hubbard B.P., Mercken E.M., Palmeira C.M., de Cabo R., Rolo A.P., Turner N., Bell E.L., Sinclair D.A. david_sinclair@hms.harvard.edu 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-myoblast Mus musculus myoblasts myoblast myoblast - a type of embryonic progenitor cell that differentiates to give rise to muscle cells ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Gomes A.P., Price N.L., Ling A.J., Moslehi J.J., Montgomery M.K., Rajman L., White J.P., Teodoro J.S., Wrann C.D., Hubbard B.P., Mercken E.M., Palmeira C.M., de Cabo R., Rolo A.P., Turner N., Bell E.L., Sinclair D.A. david_sinclair@hms.harvard.edu Accepted 2014-FEB-12 by MGT Cell (0092-8674) 2013 Only protein-protein interactions imex curation Declining NAD(+) Induces a Pseudohypoxic State Disrupting Nuclear-Mitochondrial Communication during Aging. Declining NAD(+) Induces a Pseudohypoxic State Disrupting Nuclear-Mitochondrial Communication during Aging. Cell (0092-8674) 2013 imex curation Gomes A.P., Price N.L., Ling A.J., Moslehi J.J., Montgomery M.K., Rajman L., White J.P., Teodoro J.S., Wrann C.D., Hubbard B.P., Mercken E.M., Palmeira C.M., de Cabo R., Rolo A.P., Turner N., Bell E.L., Sinclair D.A. david_sinclair@hms.harvard.edu 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-myoblast Mus musculus myoblasts myoblast myoblast - a type of embryonic progenitor cell that differentiates to give rise to muscle cells crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Gomes A.P., Price N.L., Ling A.J., Moslehi J.J., Montgomery M.K., Rajman L., White J.P., Teodoro J.S., Wrann C.D., Hubbard B.P., Mercken E.M., Palmeira C.M., de Cabo R., Rolo A.P., Turner N., Bell E.L., Sinclair D.A. DSP was used as a Crosslinking agent followed by IP. david_sinclair@hms.harvard.edu Accepted 2014-FEB-12 by MGT Cell (0092-8674) 2013 Only protein-protein interactions imex curation tfam_mouse_gene Transcription factor A, mitochondrial Tfam Hmgts Testis-specific high mobility group protein gene gene mouse Mus musculus Mouse ldha_mouse_gene Mouse L-lactate dehydrogenase A chain gene gene gene mouse Mus musculus Mouse hif1a_mouse Hypoxia-inducible factor 1-alpha Hif1a ARNT-interacting protein protein protein mouse Mus musculus Mouse MEGAGGENEKKKMSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGGLDSEDEMKAQMDCFYLKALDGFVMVLTDDGDMVYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKELNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDALTLLAPAAGDTIISLDFGSDDTETEDQQLEDVPLYNDVMFPSSNEKLNINLAMSPLPSSETPKPLRSSADPALNQEVALKLESSPESLGLSFTMPQIQDQPASPSDGSTRQSSPERLLQENVNTPNFSQPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSMSTVTGFQQTQLQKPTITATATTTATTDESKTETKDNKEDIKILIASPSSTQVPQETTTAKASAYSGTHSRTASPDRAGKRVIEQTDKAHPRSLNLSATLNQRNTVPEEELNPKTIASQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDCAPTMSLSWKRVKGFISSEQNGTEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN 84D64ECAC2CC753B myc_mouse Myc proto-oncogene protein Proto-oncogene c-Myc Transcription factor p64 Myc protein protein mouse Mus musculus Mouse MPLNVNFTNRNYDLDYDSVQPYFICDEEENFYHQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVATSFSPREDDDGGGGNFSTADQLEMMTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSTSLSPARGHSVCSTSSLYLQDLTAAASECIDPSVVFPYPLNDSSSPKSCTSSDSTAFSPSSDSLLSSESSPRASPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQTPAKRSESGSSPSRGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRAKLDSGRVLKQISNNRKCSSPRSSDTEENDKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSIQADEHKLTSEKDLLRKRREQLKHKLEQLRNSGA DIP protein P01108 original sequence version: 146 DIP protein P01108 original sequence version: 167 3FCA39BFFD6FC59E hif1a-myc 2 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Fig. 5G Deletion of SIRT1 in primary myoblasts increased the binding between HIF-1α and c-Myc. myc-tfam 1 6 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 5M, 5N, 5O. Binding of c-Myc to the TFAM promoter was markedly reduced when SIRT1 was deleted, but not when HIF-1a was also knocked down. P01108 increases expression at RNA level of ENSMUSG00000003923 hif1a-ldha-1 1 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Binding was increased in Sirt1-/- mice Supp Figs. 5M, 5N