IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Single-step protease cleavage elution for identification of protein-protein interactions from GST pull-down and mass spectrometry. Single-step protease cleavage elution for identification of protein-protein interactions from GST pull-down and mass spectrometry. Proteomics (1615-9853) 2014 imex curation Luo L., King N.P., Yeo J.C., Jones A., Stow J.L. l.luo@imb.uq.edu.au 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-raw264_7 Mus musculus monocyte macrophage cell line RAW264 raw264_7 Mouse monocyte macrophage cellline RAW264.7 pull down pull down sequence tag sequence tag identification sequence tag Luo L., King N.P., Yeo J.C., Jones A., Stow J.L. Proteins were released from the GSH-beads by selective protease cleavage using PreScission protease. Only those preys of mouse origin and with a MASCOT score above that of known contaminants have been included. l.luo@imb.uq.edu.au Accepted 2014-FEB-25 by ORCHARD Proteomics (1615-9853) 2014 imex curation Single-step protease cleavage elution for identification of protein-protein interactions from GST pull-down and mass spectrometry. Single-step protease cleavage elution for identification of protein-protein interactions from GST pull-down and mass spectrometry. Proteomics (1615-9853) 2014 imex curation Luo L., King N.P., Yeo J.C., Jones A., Stow J.L. l.luo@imb.uq.edu.au 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-raw264_7 Mus musculus monocyte macrophage cell line RAW264 raw264_7 Mouse monocyte macrophage cellline RAW264.7 pull down pull down western blot western blot Immuno blot Immuno blot Luo L., King N.P., Yeo J.C., Jones A., Stow J.L. no Proteins were released from the GSH-beads by selective protease cleavage using PreScission protease. l.luo@imb.uq.edu.au Accepted 2014-FEB-25 by ORCHARD Proteomics (1615-9853) 2014 imex curation Single-step protease cleavage elution for identification of protein-protein interactions from GST pull-down and mass spectrometry. Single-step protease cleavage elution for identification of protein-protein interactions from GST pull-down and mass spectrometry. Proteomics (1615-9853) 2014 imex curation Luo L., King N.P., Yeo J.C., Jones A., Stow J.L. l.luo@imb.uq.edu.au 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-raw264_7 Mus musculus monocyte macrophage cell line RAW264 raw264_7 Mouse monocyte macrophage cellline RAW264.7 pull down pull down sequence tag sequence tag identification sequence tag Luo L., King N.P., Yeo J.C., Jones A., Stow J.L. Proteins were released from the GSH-beads by boiling the sample. Only those preys of mouse origin and with a MASCOT score above that of known contaminants have been included. l.luo@imb.uq.edu.au Accepted 2014-FEB-25 by ORCHARD Proteomics (1615-9853) 2014 imex curation Single-step protease cleavage elution for identification of protein-protein interactions from GST pull-down and mass spectrometry. Single-step protease cleavage elution for identification of protein-protein interactions from GST pull-down and mass spectrometry. Proteomics (1615-9853) 2014 imex curation Luo L., King N.P., Yeo J.C., Jones A., Stow J.L. l.luo@imb.uq.edu.au 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-raw264_7 Mus musculus monocyte macrophage cell line RAW264 raw264_7 Mouse monocyte macrophage cellline RAW264.7 pull down pull down western blot western blot Immuno blot Immuno blot Luo L., King N.P., Yeo J.C., Jones A., Stow J.L. no Proteins were released from the GSH-beads by boiling the sample. l.luo@imb.uq.edu.au Accepted 2014-FEB-25 by ORCHARD Proteomics (1615-9853) 2014 imex curation atpa_mouse ATP synthase subunit alpha, mitochondrial Atp5f1a ATP synthase F1 subunit alpha Atp5a1 protein protein mouse Mus musculus Mouse MLSVRVAAAVARALPRRAGLVSKNALGSSFVGARNLHASNTRLQKTGTAEMSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDVVKRTGAIVDVPVGEELLGRVVDALGNAIDGKGPIGSKTRRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIGRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGTDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDSFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREVAAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVRGYLDKLEPSKITKFENAFLSHVISQHQSLLGNIRSDGKISEQSDAKLKEIVTNFLAGFEP CF35B4FBA7ED431D atpb_mouse ATP synthase subunit beta, mitochondrial Atp5f1b ATP synthase F1 subunit beta Atp5b protein protein mouse Mus musculus Mouse MLSLVGRVASASASGALRGLSPSAALPQAQLLLRAAPAGVHPARDYAAQASAAPKAGTATGRIVAVIGAVVDVQFDEGLPPILNALEVQGRDSRLVLEVAQHLGESTVRTIAMDGTEGLVRGQKVLDSGAPIKIPVGPETLGRIMNVIGEPIDERGPIKTKQFAPIHAEAPEFIEMSVEQEILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGNEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFQVAEVFTGHMGKLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEEHGS F3E1100C390A78A7 eea1_mouse Early endosome antigen 1 Eea1 protein protein mouse Mus musculus Mouse MFRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQCMKSLGSADELFKHYQAVHDAGNDSGHGGEAGLALTRDDITLLRQEVQDLQASLKEEKWYSEELKKELEKYQGLQQQEAKSDGLVTDSSAELQALEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEEKSLRAAAEQKVTHLTEDLNKQTTVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSEHAHLEATINQLRSELAKGPQEVAVYVQEIQKLKGSINELTQKNQNLTEKLQKKDLDYTHLEEKHNEESASRKTLQASLHQRDLDCQQLQARLTASESSLQRAQGELSEKAEAAQKLREELREVESTRQHLKVEVKQLQQQREEKEQHGLQLQGEVSQLHCKLLETERQLGEAHGRLKEQRQLSSEKLMEKEQQVADLQLKLSRLEEQLKEKVTNSTELQHQLEKSKQQHQEQQALQQSATAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKGKESVSLLEKEREDLYAKIQAGEGETAVLNQLQEKNHALQQQLTQLTEKLKNQSESHKQAEENLHDQVQEQKAHLRAAQDRVLSLETSVSELSSQLNESKEKVSQLDIQIKAKTELLLSAEAAKAAQRADLQNHLDTAQHALQDKQQELNKVSVQLDQLTAKFQEKQEHCIQLESHLKDHKEKHLSLEQKVEDLEGHIKKLEADALEVKASKEQALQSLQQQRQLSTDLELRNAELSRELQEQEEVVSCTKLDLQNKSEILENIKQTLTKKEEENVVLKQEFEKLSQDSKTQHKELGDRMQAAVTELTAVKAQKDALLAELSTTKEKLSKVSDSLKNSKSEFEKENQKGKAAVLDLEKACKELKHQLQVQAESALKEQEDLKKSLEKEKETSQQLKIELNSVKGEVSQAQNTLKQKEKDEQQLQGTINQLKQSAEQKKKQIEALQGEVKNAVSQKTVLENKLQQQSSQAAQELAAEKGKLSALQSNYEKCQADLKQLQSDLYGKESELLATRQDLKSVEEKLTLAQEDLISNRNQIGNQNKSIQELQAAKASLEQDSAKKEALLKEQSKALEDAQREKSVKEKELVAEKSKLAEMEEIKCRQEKEITKLNEELKSHKQESIKEITNLKDAKQLLIQQKLELQGRVDSLKAALEQEKESQQLMREQVKKEEEKRKEEFSEKEAKLHSEIKEKEAGMKKHEENEAKLTMQVTTLNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKALELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMSCGKCFSVTVRRHHCRQCGNIFCAECSTKNALTPSSKKPVRVCDACFNDLQG 2365A51EF92019FD rab31_mouse Ras-related protein Rab-31 Rab31 protein protein mouse Mus musculus Mouse MAIRELKVCLLGDTGVGKSSIVCRFVQDHFDHNISPTIGASFMTKTVPCGNELHKFLIWDTAGQERFHSLAPMYYRGSAAAVIVYDITKQDSFHTLKKWVKELKEHGPENIVMAIAGNKCDLSDIREVPLKDAKEYAESIGAIVVETSAKNAINIEELFQGISRQIPPLGPQENGNSGGIKLGNQSLQASRRCC mint 4EBCAF6E2431AD02 rab31-eea1-2 4 8 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 7 unspecified role unspecified role prey prey association association 2b, 2c Actin was also detected in this interaction, but could not be identified which specific actin was pulled-down, so it was not included. rab31-eea1-1 2 8 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 7 unspecified role unspecified role prey prey association association 2b, 2c Actin was also detected in this interaction, but could not be identified which specific actin was pulled-down, so it was not included. rab31-atp5a1-2 1 8 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey association association Supplementary table 1 rab31-atp5a1-1 3 8 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey association association Supplementary table 1