MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Toll-like receptor-mediated IRE1α activation as a therapeutic target for inflammatory arthritis. Toll-like receptor-mediated IRE1α activation as a therapeutic target for inflammatory arthritis. EMBO J. (0261-4189) 2013 imex curation Qiu Q., Zheng Z., Chang L., Zhao Y.S., Tan C., Dandekar A., Zhang Z., Lin Z., Gui M., Li X., Zhang T., Kong Q., Li H., Chen S., Chen A., Kaufman R.J., Yang W.L., Lin H.K., Zhang D., Perlman H., Thorp E., Zhang K., Fang D. kzhang@med.wayne.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Qiu Q., Zheng Z., Chang L., Zhao Y.S., Tan C., Dandekar A., Zhang Z., Lin Z., Gui M., Li X., Zhang T., Kong Q., Li H., Chen S., Chen A., Kaufman R.J., Yang W.L., Lin H.K., Zhang D., Perlman H., Thorp E., Zhang K., Fang D. kzhang@med.wayne.edu Accepted 2015-FEB-09 by LUANA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation Toll-like receptor-mediated IRE1α activation as a therapeutic target for inflammatory arthritis. Toll-like receptor-mediated IRE1α activation as a therapeutic target for inflammatory arthritis. EMBO J. (0261-4189) 2013 imex curation Qiu Q., Zheng Z., Chang L., Zhao Y.S., Tan C., Dandekar A., Zhang Z., Lin Z., Gui M., Li X., Zhang T., Kong Q., Li H., Chen S., Chen A., Kaufman R.J., Yang W.L., Lin H.K., Zhang D., Perlman H., Thorp E., Zhang K., Fang D. kzhang@med.wayne.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Qiu Q., Zheng Z., Chang L., Zhao Y.S., Tan C., Dandekar A., Zhang Z., Lin Z., Gui M., Li X., Zhang T., Kong Q., Li H., Chen S., Chen A., Kaufman R.J., Yang W.L., Lin H.K., Zhang D., Perlman H., Thorp E., Zhang K., Fang D. kzhang@med.wayne.edu Accepted 2015-FEB-09 by LUANA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation Toll-like receptor-mediated IRE1α activation as a therapeutic target for inflammatory arthritis. Toll-like receptor-mediated IRE1α activation as a therapeutic target for inflammatory arthritis. EMBO J. (0261-4189) 2013 imex curation Qiu Q., Zheng Z., Chang L., Zhao Y.S., Tan C., Dandekar A., Zhang Z., Lin Z., Gui M., Li X., Zhang T., Kong Q., Li H., Chen S., Chen A., Kaufman R.J., Yang W.L., Lin H.K., Zhang D., Perlman H., Thorp E., Zhang K., Fang D. kzhang@med.wayne.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions mouse-raw-1 Mus musculus raw-1 raw-1 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Qiu Q., Zheng Z., Chang L., Zhao Y.S., Tan C., Dandekar A., Zhang Z., Lin Z., Gui M., Li X., Zhang T., Kong Q., Li H., Chen S., Chen A., Kaufman R.J., Yang W.L., Lin H.K., Zhang D., Perlman H., Thorp E., Zhang K., Fang D. kzhang@med.wayne.edu Accepted 2015-FEB-09 by LUANA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation Toll-like receptor-mediated IRE1α activation as a therapeutic target for inflammatory arthritis. Toll-like receptor-mediated IRE1α activation as a therapeutic target for inflammatory arthritis. EMBO J. (0261-4189) 2013 imex curation Qiu Q., Zheng Z., Chang L., Zhao Y.S., Tan C., Dandekar A., Zhang Z., Lin Z., Gui M., Li X., Zhang T., Kong Q., Li H., Chen S., Chen A., Kaufman R.J., Yang W.L., Lin H.K., Zhang D., Perlman H., Thorp E., Zhang K., Fang D. kzhang@med.wayne.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions mouse-macrophage Mus musculus macrophage macrophage Macrophage anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Qiu Q., Zheng Z., Chang L., Zhao Y.S., Tan C., Dandekar A., Zhang Z., Lin Z., Gui M., Li X., Zhang T., Kong Q., Li H., Chen S., Chen A., Kaufman R.J., Yang W.L., Lin H.K., Zhang D., Perlman H., Thorp E., Zhang K., Fang D. kzhang@med.wayne.edu Accepted 2015-FEB-09 by LUANA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation traf6_mouse TNF receptor-associated factor 6 RING-type E3 ubiquitin transferase TRAF6 Traf6 E3 ubiquitin-protein ligase TRAF6 protein protein mouse Mus musculus Mouse MSLLNCENSCGSSQSSSDCCAAMAASCSAAVKDDSVSGSASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLTVKCPNKGCLQKMELRHLEDHQVHCEFALVNCPQCQRPFQKCQVNTHIIEDCPRRQVSCVNCAVSMAYEEKEIHDQSCPLANIICEYCGTILIREQMPNHYDLDCPTAPIPCTFSVFGCHEKMQRNHLARHLQENTQLHMRLLAQAVHNVNLALRPCDAASPSRGCRPEDPNYEETIKQLESRLVRQDHQIRELTAKMETQSMYVGELKRTIRTLEDKVAEMEAQQCNGIYIWKIGNFGMHLKSQEEERPVVIHSPGFYTGRPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEALIRQNHEEVMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEALRQGTFIKDDTLLVRCEVSTRFDMGGLRKEGFQPRSTDAGV DIP protein P70196 original sequence version: 139 DIP protein P70196 original sequence version: 128 DIP protein P70196 original sequence version: 144 DIP protein P70196 original sequence version: 83 DIP protein P70196 original sequence version: 98 DIP protein P70196 original sequence version: 104 DIP protein P70196 original sequence version: 112 DIP protein P70196 original sequence version: 163 F8389250425E4E2D ern1_mouse Serine/threonine-protein kinase/endoribonuclease IRE1 Ern1 Ire1 Endoplasmic reticulum-to-nucleus signaling 1 Inositol-requiring protein 1 Ire1-alpha protein protein mouse Mus musculus Mouse MPARWLLLLLALLLPPPGPGSFGRTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEEPAFLPDPNDGSLYTLGGKNNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQGEVLRKVVHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDLKFDPGLKGKSKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKRSFEEVINIVGQTSDNTPTTVSQDVEEKLARAPAKPEAPVDSMLKDMATIILSTFLLVGWVAFIITYPLSVHQQRQLQHQQFQKELEKIQLLQQQQLPFHPHGDLTQDPEFLDSSGPFSESSGTSSPSPSPRASNHSLHPSSSASRAGTSPSLEQDDEDEETRMVIVGKISFCPKDVLGHGAEGTIVYKGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLHQTTSGLAHLHSLNIVHRDLKPHNILLSMPNAHGRIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKDNPTYTVDIFSAGCVFYYVISEGNHPFGKSLQRQANILLGACNLDCFHSDKHEDVIARELIEKMIAMDPQQRPSAKHVLKHPFFWSLEKQLQFFQDVSDRIEKEALDGPIVRQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHHYRELPVEVQETLGSIPDDFVRYFTSRFPHLLSHTYQAMELCRHERLFQTYYWHEPTEPQPPVIPYAL 216E3E2FA2FF3F70 ern1-traf6 2 6 anti tag western anti tag western blot anti tag western anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 anti tag western anti tag western blot anti tag western anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association F5A ern1-traf6-1 4 6 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 5 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association F5B traf6-ern1 3 6 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 5 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association F5c traf6-ern1-1 2 6 anti tag western anti tag western blot anti tag western anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 anti tag western anti tag western blot anti tag western anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association F5d ern1-traf6-2 2 6 anti tag western anti tag western blot anti tag western anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 5 anti tag western anti tag western blot anti tag western anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association F5e ern1-traf6-3 1 5 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell P70196:p.Cys70Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain C A Wed May 02 18:58:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys70ala' Tue Jun 30 18:07:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys70Ala' flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell physical association physical association F6f