MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it An ER-peroxisome tether exerts peroxisome population control in yeast. An ER-peroxisome tether exerts peroxisome population control in yeast. EMBO J. (0261-4189) 2013 imex curation Knoblach B., Sun X., Coquelle N., Fagarasanu A., Poirier R.L., Rachubinski R.A. knoblach@ualberta.ca,rick.rachubinski@ualberta.ca 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Knoblach B., Sun X., Coquelle N., Fagarasanu A., Poirier R.L., Rachubinski R.A. knoblach@ualberta.ca,rick.rachubinski@ualberta.ca knoblach@ualberta.ca; rick.rachubinski@ualberta.ca Accepted 2014-DEC-11 by LUANA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation An ER-peroxisome tether exerts peroxisome population control in yeast. An ER-peroxisome tether exerts peroxisome population control in yeast. EMBO J. (0261-4189) 2013 imex curation Knoblach B., Sun X., Coquelle N., Fagarasanu A., Poirier R.L., Rachubinski R.A. knoblach@ualberta.ca,rick.rachubinski@ualberta.ca 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Knoblach B., Sun X., Coquelle N., Fagarasanu A., Poirier R.L., Rachubinski R.A. knoblach@ualberta.ca,rick.rachubinski@ualberta.ca knoblach@ualberta.ca; rick.rachubinski@ualberta.ca Accepted 2014-DEC-11 by LUANA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation An ER-peroxisome tether exerts peroxisome population control in yeast. An ER-peroxisome tether exerts peroxisome population control in yeast. EMBO J. (0261-4189) 2013 imex curation Knoblach B., Sun X., Coquelle N., Fagarasanu A., Poirier R.L., Rachubinski R.A. knoblach@ualberta.ca,rick.rachubinski@ualberta.ca 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati western blot western blot Immuno blot Immuno blot Knoblach B., Sun X., Coquelle N., Fagarasanu A., Poirier R.L., Rachubinski R.A. knoblach@ualberta.ca,rick.rachubinski@ualberta.ca knoblach@ualberta.ca; rick.rachubinski@ualberta.ca Accepted 2014-DEC-11 by LUANA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation An ER-peroxisome tether exerts peroxisome population control in yeast. An ER-peroxisome tether exerts peroxisome population control in yeast. EMBO J. (0261-4189) 2013 imex curation Knoblach B., Sun X., Coquelle N., Fagarasanu A., Poirier R.L., Rachubinski R.A. knoblach@ualberta.ca,rick.rachubinski@ualberta.ca 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Knoblach B., Sun X., Coquelle N., Fagarasanu A., Poirier R.L., Rachubinski R.A. knoblach@ualberta.ca,rick.rachubinski@ualberta.ca knoblach@ualberta.ca; rick.rachubinski@ualberta.ca Accepted 2014-DEC-11 by LUANA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation An ER-peroxisome tether exerts peroxisome population control in yeast. An ER-peroxisome tether exerts peroxisome population control in yeast. EMBO J. (0261-4189) 2013 imex curation Knoblach B., Sun X., Coquelle N., Fagarasanu A., Poirier R.L., Rachubinski R.A. knoblach@ualberta.ca,rick.rachubinski@ualberta.ca 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Knoblach B., Sun X., Coquelle N., Fagarasanu A., Poirier R.L., Rachubinski R.A. knoblach@ualberta.ca,rick.rachubinski@ualberta.ca knoblach@ualberta.ca; rick.rachubinski@ualberta.ca Accepted 2014-DEC-11 by LUANA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation psb6_yeast Proteasome subunit beta type-6 PRE7 PRS3 PTS1 YBL0407 YBL041W Proteasome component C5 Multicatalytic endopeptidase complex subunit C5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MATIASEYSSEASNTPIEHQFNPYGDNGGTILGIAGEDFAVLAGDTRNITDYSINSRYEPKVFDCGDNIVMSANGFAADGDALVKRFKNSVKWYHFDHNDKKLSINSAARNIQHLLYGKRFFPYYVHTIIAGLDEDGKGAVYSFDPVGSYEREQCRAGGAAASLIMPFLDNQVNFKNQYEPGTNGKVKKPLKYLSVEEVIKLVRDSFTSATERHIQVGDGLEILIVTKDGVRKEFYELKRD 770A4DF1DF0A0F0E 770A4DF1DF0A0F0E inp1_yeast Inheritance of peroxisomes protein 1 INP1 YM8325.05C YMR204C protein protein yeast Saccharomyces cerevisiae Baker's yeast MVLSRGETKKNSVRLTAKQEKKPQSTFQTLKQSLKLSNNKKLKQDSTQHSNDTNKSVKAKKNGTSSKKTGTQRKRISTQRFSLFTYGNVQVMNSFVPIHNDIPNSSCIRRNSQVSANNVTESSGVFFNDTQSQDSQNTIKLKPTSLMAKGPIEIYQICTGFDKLKENIAPFQKSSKASSHDGHVVNYLSIGRHGDIVHPVLPKLQITRLNGAGFKYFISFYNPERYWEIEFLPLISQSQSELENSVKAFENVISKICQFSHINEGATIGNNESLSDKFKLPPTSDIEPPNTEIINNDDDNDDDDDNYDDDDLNYLLDEEYEQGCTDNSFSVISNTCSNLNASFLYPSDPTDAVSISINEAFKNAIRRTAPVLNIPIAAPSIHSKQQNKRYSSYPFIDSPPYLQDRHRRFQRRSISGLGDL A11EF51211DEF675 pex3_yeast Peroxisomal biogenesis factor 3 PEX3 PAS3 YDR329C D9798.15 Peroxin-3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAPNQRSRSLLQRHRGKVLISLTGIAALFTTGSVVVFFVKRWLYKQQLRITEQHFIKEQIKRRFEQTQEDSLYTIYELLPVWRMVLNENDLNLDSIVTQLKDQKNQLTRAKSSESRESSPLKSKAELWNELELKSLIKLVTVTYTVSSLILLTRLQLNILTRNEYLDSAIKLTMQQENCNKLQNRFYNWVTSWWSDPEDKADDAMVMAAKKSKKEGQEVYINEQAFLSLSWWILNKGWLSYNEIITNQIEIEFDGIHPRDTLTLEEFSSRLTNIFRNTNSQIFQQNNNNLTSILLPKDSSGQEFLLSQTLDADALTSFHSNTLVFNQLVNELTQCIESTATSIVLESLINESFHFIMNKVGIKTIAKKKPGQEDQQQYQMAVFAMSMKDCCQEMLQTTAGSSHSGSVNEYLATLDSVQPLDDLSASVYSNFGVSSSFSFKP 3CDC1D036DF0D0F9 3CDC1D036DF0D0F9 pex3-inp1 5 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell 8 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P28795:p.Val81Glu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain V E Wed May 02 20:00:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val81glu' Tue Jun 30 16:47:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val81Glu' physical association physical association f1c pex3-inp1-1 5 7 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell 8 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell P28795:p.Asn188Ile mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain N I Wed May 02 18:51:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn188ile' Tue Jun 30 16:58:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn188Ile' range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association f1c inp1-pex3-1 4 8 tag fluorescence tag visualisation by fluorescence tag fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell P28795:p.Val81Glu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain V E Wed May 02 19:38:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val81glu' Tue Jun 30 15:38:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val81Glu' mcherry tag mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 tag fluorescence tag visualisation by fluorescence tag fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f2a f2b inp1-pex3-1 4 6 tag fluorescence tag visualisation by fluorescence tag fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell mcherry tag mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 tag fluorescence tag visualisation by fluorescence tag fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f2a inp1-pex3-1 3 8 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed P28795:p.Val81Glu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain V E Wed May 02 17:01:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val81glu' Tue Jun 30 16:58:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val81Glu' 7 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f2c inp1-pex3-1 1 8 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed P28795:p.Val81Glu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain V E Wed May 02 16:04:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val81glu' Tue Jun 30 20:23:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val81Glu' 7 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f2d inp1-pex3-1 2 8 anti tag western anti tag western blot anti tag western anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 anti tag western anti tag western blot anti tag western anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f4