IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Kallikrein-related peptidase 5 functions in proteolytic processing of profilaggrin in cultured human keratinocytes. Kallikrein-related peptidase 5 functions in proteolytic processing of profilaggrin in cultured human keratinocytes. J. Biol. Chem. (0021-9258) 2013 imex curation Sakabe J., Yamamoto M., Hirakawa S., Motoyama A., Ohta I., Tatsuno K., Ito T., Kabashima K., Hibino T., Tokura Y. I would think the UniProt entry is for Pro-filaggrin - it has the two EF hand regions.... jsakabe@hama-med.ac.jp 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protease assay protease assay western blot western blot Immuno blot Immuno blot Sakabe J., Yamamoto M., Hirakawa S., Motoyama A., Ohta I., Tatsuno K., Ito T., Kabashima K., Hibino T., Tokura Y. The sequence used here for "Recombinant Human Filaggrin Fusion Protein" is a 'best guess' - as the exact sequence is not given. Mapping for the linker sequence is taken from the most C-terminal instance found. I would think the UniProt entry is for Pro-filaggrin - it has the two EF hand regions.... jsakabe@hama-med.ac.jp Accepted 2014-AUG-05 by ORCHARD J. Biol. Chem. (0021-9258) 2013 Only protein-protein interactions imex curation Kallikrein-related peptidase 5 functions in proteolytic processing of profilaggrin in cultured human keratinocytes. Kallikrein-related peptidase 5 functions in proteolytic processing of profilaggrin in cultured human keratinocytes. J. Biol. Chem. (0021-9258) 2013 imex curation Sakabe J., Yamamoto M., Hirakawa S., Motoyama A., Ohta I., Tatsuno K., Ito T., Kabashima K., Hibino T., Tokura Y. I would think the UniProt entry is for Pro-filaggrin - it has the two EF hand regions.... jsakabe@hama-med.ac.jp 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-epidermis HUMAN EPDERMIS epidermis A thin surface layer of tissue in higher plants formed by growth of a primary meristem. pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Sakabe J., Yamamoto M., Hirakawa S., Motoyama A., Ohta I., Tatsuno K., Ito T., Kabashima K., Hibino T., Tokura Y. I would think the UniProt entry is for Pro-filaggrin - it has the two EF hand regions.... jsakabe@hama-med.ac.jp Accepted 2014-AUG-05 by ORCHARD J. Biol. Chem. (0021-9258) 2013 Only protein-protein interactions imex curation Kallikrein-related peptidase 5 functions in proteolytic processing of profilaggrin in cultured human keratinocytes. Kallikrein-related peptidase 5 functions in proteolytic processing of profilaggrin in cultured human keratinocytes. J. Biol. Chem. (0021-9258) 2013 imex curation Sakabe J., Yamamoto M., Hirakawa S., Motoyama A., Ohta I., Tatsuno K., Ito T., Kabashima K., Hibino T., Tokura Y. I would think the UniProt entry is for Pro-filaggrin - it has the two EF hand regions.... jsakabe@hama-med.ac.jp 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-epidermis HUMAN EPDERMIS epidermis A thin surface layer of tissue in higher plants formed by growth of a primary meristem. fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Sakabe J., Yamamoto M., Hirakawa S., Motoyama A., Ohta I., Tatsuno K., Ito T., Kabashima K., Hibino T., Tokura Y. I would think the UniProt entry is for Pro-filaggrin - it has the two EF hand regions.... jsakabe@hama-med.ac.jp Accepted 2014-AUG-05 by ORCHARD J. Biol. Chem. (0021-9258) 2013 Only protein-protein interactions imex curation Kallikrein-related peptidase 5 functions in proteolytic processing of profilaggrin in cultured human keratinocytes. Kallikrein-related peptidase 5 functions in proteolytic processing of profilaggrin in cultured human keratinocytes. J. Biol. Chem. (0021-9258) 2013 imex curation Sakabe J., Yamamoto M., Hirakawa S., Motoyama A., Ohta I., Tatsuno K., Ito T., Kabashima K., Hibino T., Tokura Y. I would think the UniProt entry is for Pro-filaggrin - it has the two EF hand regions.... jsakabe@hama-med.ac.jp 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-epidermis HUMAN EPDERMIS epidermis A thin surface layer of tissue in higher plants formed by growth of a primary meristem. electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy antibody detection identification by antibody antibody detection Sakabe J., Yamamoto M., Hirakawa S., Motoyama A., Ohta I., Tatsuno K., Ito T., Kabashima K., Hibino T., Tokura Y. I would think the UniProt entry is for Pro-filaggrin - it has the two EF hand regions.... Skin specimens were treated with 10- (KLK5) and 40-(profilaggrin) nm gold particle-conjugated secondary antibodies. jsakabe@hama-med.ac.jp Accepted 2014-AUG-05 by ORCHARD J. Biol. Chem. (0021-9258) 2013 Only protein-protein interactions imex curation fila_human Filaggrin FLG protein protein human Homo sapiens Human MSTLLENIFAIINLFKQYSKKDKNTDTLSKKELKELLEKEFRQILKNPDDPDMVDVFMDHLDIDHNKKIDFTEFLLMVFKLAQAYYESTRKENLPISGHKHRKHSHHDKHEDNKQEENKENRKRPSSLERRNNRKGNKGRSKSPRETGGKRHESSSEKKERKGYSPTHREEEYGKNHHNSSKKEKNKTENTRLGDNRKRLSERLEEKEDNEEGVYDYENTGRMTQKWIQSGHIATYYTIQDEAYDTTDSLLEENKIYERSRSSDGKSSSQVNRSRHENTSQVPLQESRTRKRRGSRVSQDRDSEGHSEDSERHSGSASRNHHGSAWEQSRDGSRHPRSHDEDRASHGHSADSSRQSGTRHAETSSRGQTASSHEQARSSPGERHGSGHQQSADSSRHSATGRGQASSAVSDRGHRGSSGSQASDSEGHSENSDTQSVSGHGKAGLRQQSHQESTRGRSGERSGRSGSSLYQVSTHEQPDSAHGRTGTSTGGRQGSHHEQARDSSRHSASQEGQDTIRGHPGSSRGGRQGSHHEQSVNRSGHSGSHHSHTTSQGRSDASHGQSGSRSASRQTRNEEQSGDGTRHSGSRHHEASSQADSSRHSQVGQGQSSGPRTSRNQGSSVSQDSDSQGHSEDSERWSGSASRNHHGSAQEQSRDGSRHPRSHHEDRAGHGHSADSSRKSGTRHTQNSSSGQAASSHEQARSSAGERHGSRHQLQSADSSRHSGTGHGQASSAVRDSGHRGSSGSQATDSEGHSEDSDTQSVSGHGQAGHHQQSHQESARDRSGERSRRSGSFLYQVSTHKQSESSHGWTGPSTGVRQGSHHEQARDNSRHSASQDGQDTIRGHPGSSRRGRQGSHHEQSVDRSGHSGSHHSHTTSQGRSDASRGQSGSRSASRTTRNEEQSRDGSRHSGSRHHEASSHADISRHSQAGQGQSEGSRTSRRQGSSVSQDSDSEGHSEDSERWSGSASRNHRGSAQEQSRHGSRHPRSHHEDRAGHGHSADSSRQSGTPHAETSSGGQAASSHEQARSSPGERHGSRHQQSADSSRHSGIPRRQASSAVRDSGHWGSSGSQASDSEGHSEESDTQSVSGHGQDGPHQQSHQESARDWSGGRSGRSGSFIYQVSTHEQSESAHGRTRTSTGRRQGSHHEQARDSSRHSASQEGQDTIRAHPGSRRGGRQGSHHEQSVDRSGHSGSHHSHTTSQGRSDASHGQSGSRSASRQTRKDKQSGDGSRHSGSRHHEAASWADSSRHSQVGQEQSSGSRTSRHQGSSVSQDSDSERHSDDSERLSGSASRNHHGSSREQSRDGSRHPGFHQEDRASHGHSADSSRQSGTHHTESSSHGQAVSSHEQARSSPGERHGSRHQQSADSSRHSGIGHRQASSAVRDSGHRGSSGSQVTNSEGHSEDSDTQSVSAHGQAGPHQQSHKESARGQSGESSGRSRSFLYQVSSHEQSESTHGQTAPSTGGRQGSRHEQARNSSRHSASQDGQDTIRGHPGSSRGGRQGSYHEQSVDRSGHSGYHHSHTTPQGRSDASHGQSGPRSASRQTRNEEQSGDGSRHSGSRHHEPSTRAGSSRHSQVGQGESAGSKTSRRQGSSVSQDRDSEGHSEDSERRSESASRNHYGSAREQSRHGSRNPRSHQEDRASHGHSAESSRQSGTRHAETSSGGQAASSQEQARSSPGERHGSRHQQSADSSTDSGTGRRQDSSVVGDSGNRGSSGSQASDSEGHSEESDTQSVSAHGQAGPHQQSHQESTRGQSGERSGRSGSFLYQVSTHEQSESAHGRTGPSTGGRQRSRHEQARDSSRHSASQEGQDTIRGHPGSSRGGRQGSHYEQSVDSSGHSGSHHSHTTSQERSDVSRGQSGSRSVSRQTRNEKQSGDGSRHSGSRHHEASSRADSSRHSQVGQGQSSGPRTSRNQGSSVSQDSDSQGHSEDSERWSGSASRNHLGSAWEQSRDGSRHPGSHHEDRAGHGHSADSSRQSGTRHTESSSRGQAASSHEQARSSAGERHGSHHQLQSADSSRHSGIGHGQASSAVRDSGHRGYSGSQASDSEGHSEDSDTQSVSAQGKAGPHQQSHKESARGQSGESSGRSGSFLYQVSTHEQSESTHGQSAPSTGGRQGSHYDQAQDSSRHSASQEGQDTIRGHPGPSRGGRQGSHQEQSVDRSGHSGSHHSHTTSQGRSDASRGQSGSRSASRKTYDKEQSGDGSRHSGSHHHEASSWADSSRHSLVGQGQSSGPRTSRPRGSSVSQDSDSEGHSEDSERRSGSASRNHHGSAQEQSRDGSRHPRSHHEDRAGHGHSAESSRQSGTHHAENSSGGQAASSHEQARSSAGERHGSHHQQSADSSRHSGIGHGQASSAVRDSGHRGSSGSQASDSEGHSEDSDTQSVSAHGQAGPHQQSHQESTRGRSAGRSGRSGSFLYQVSTHEQSESAHGRTGTSTGGRQGSHHKQARDSSRHSTSQEGQDTIHGHPGSSSGGRQGSHYEQLVDRSGHSGSHHSHTTSQGRSDASHGHSGSRSASRQTRNDEQSGDGSRHSGSRHHEASSRADSSGHSQVGQGQSEGPRTSRNWGSSFSQDSDSQGHSEDSERWSGSASRNHHGSAQEQLRDGSRHPRSHQEDRAGHGHSADSSRQSGTRHTQTSSGGQAASSHEQARSSAGERHGSHHQQSADSSRHSGIGHGQASSAVRDSGHRGYSGSQASDNEGHSEDSDTQSVSAHGQAGSHQQSHQESARGRSGETSGHSGSFLYQVSTHEQSESSHGWTGPSTRGRQGSRHEQAQDSSRHSASQDGQDTIRGHPGSSRGGRQGYHHEHSVDSSGHSGSHHSHTTSQGRSDASRGQSGSRSASRTTRNEEQSGDGSRHSGSRHHEASTHADISRHSQAVQGQSEGSRRSRRQGSSVSQDSDSEGHSEDSERWSGSASRNHHGSAQEQLRDGSRHPRSHQEDRAGHGHSADSSRQSGTRHTQTSSGGQAASSHEQARSSAGERHGSHHQQSADSSRHSGIGHGQASSAVRDSGHRGYSGSQASDNEGHSEDSDTQSVSAHGQAGSHQQSHQESARGRSGETSGHSGSFLYQVSTHEQSESSHGWTGPSTRGRQGSRHEQAQDSSRHSASQYGQDTIRGHPGSSRGGRQGYHHEHSVDSSGHSGSHHSHTTSQGRSDASRGQSGSRSASRTTRNEEQSGDSSRHSVSRHHEASTHADISRHSQAVQGQSEGSRRSRRQGSSVSQDSDSEGHSEDSERWSGSASRNHRGSVQEQSRHGSRHPRSHHEDRAGHGHSADRSRQSGTRHAETSSGGQAASSHEQARSSPGERHGSRHQQSADSSRHSGIPRGQASSAVRDSRHWGSSGSQASDSEGHSEESDTQSVSGHGQAGPHQQSHQESARDRSGGRSGRSGSFLYQVSTHEQSESAHGRTRTSTGRRQGSHHEQARDSSRHSASQEGQDTIRGHPGSSRRGRQGSHYEQSVDRSGHSGSHHSHTTSQGRSDASRGQSGSRSASRQTRNDEQSGDGSRHSWSHHHEASTQADSSRHSQSGQGQSAGPRTSRNQGSSVSQDSDSQGHSEDSERWSGSASRNHRGSAQEQSRDGSRHPTSHHEDRAGHGHSAESSRQSGTHHAENSSGGQAASSHEQARSSAGERHGSHHQQSADSSRHSGIGHGQASSAVRDSGHRGSSGSQASDSEGHSEDSDTQSVSAHGQAGPHQQSHQESTRGRSAGRSGRSGSFLYQVSTHEQSESAHGRAGPSTGGRQGSRHEQARDSSRHSASQEGQDTIRGHPGSRRGGRQGSYHEQSVDRSGHSGSHHSHTTSQGRSDASHGQSGSRSASRETRNEEQSGDGSRHSGSRHHEASTQADSSRHSQSGQGESAGSRRSRRQGSSVSQDSDSEAYPEDSERRSESASRNHHGSSREQSRDGSRHPGSSHRDTASHVQSSPVQSDSSTAKEHGHFSSLSQDSAYHSGIQSRGSPHSSSSYHYQSEGTERQKGQSGLVWRHGSYGSADYDYGESGFRHSQHGSVSYNSNPVVFKERSDICKASAFGKDHPRYYATYINKDPGLCGHSSDISKQLGFSQSQRYYYYE 3F4B1181F04AD9C0 klk5_human Kallikrein-5 KLK5 Kallikrein-like protein 2 SCTE Stratum corneum tryptic enzyme UNQ570/PRO1132 protein protein human Homo sapiens Human MATARPPWMWVLCALITALLLGVTEHVLANNDVSCDHPSNTVPSGSNQDLGAGAGEDARSDDSSSRIINGSDCDMHTQPWQAALLLRPNQLYCGAVLVHPQWLLTAAHCRKKVFRVRLGHYSLSPVYESGQQMFQGVKSIPHPGYSHPGHSNDLMLIKLNRRIRPTKDVRPINVSSHCPSAGTKCLVSGWGTTKSPQVHFPKVLQCLNISVLSQKRCEDAYPRQIDDTMFCAGDKAGRDSCQGDSGGPVVCNGSLQGLVSWGDYPCARPNRPGVYTNLCKFTKWIQETIQANS D92C92F5609E5946 flg-klk5 1 6 enzyme enzyme neutral component neutral component peptidase s1_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mouse-nso NSO-mouse myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 5 enzyme target enzyme target substrate substrate neutral component neutral component P20930:p.[Ser3385His;Tyr3388_Gln3389delinsHisHis;Ser3391_Thr3392delinsHisHis] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain ST HH certain certain sequence position certain certain certain sequence position certain S H certain certain sequence position certain certain certain sequence position certain YQ HH Thu Jul 07 12:06:57 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was ser3385his,tyr3388his,gln3389his,ser3391his,thr3392his Fri Jul 22 10:57:59 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser3385his,tyr3388his,gln3389his,ser3391his,thr3392his' Wed May 02 17:36:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser3385his,tyr_gln3388-3389his_his,ser_thr3391-3392his_his' Tue Jun 30 19:45:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser3385His;Tyr3388_Gln3389delinsHisHis;Ser3391_Thr3392delinsHisHis]' c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range monomer(s)_with linker sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) cleavage cleavage reaction cleavage 2C, Figure 3. AEBSF, a kallikrein inhibitor. Mutating the linker sequence from SFLYQVST to HFLHHVHH stopped the cleavage. flg-klk5-1 3 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component colocalization colocalization 4 D-F Both proteins are expressed and localize in the granular layer. flg-klk5-2 2 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 4 G-H There is an association of profilaggrin and KLK5 at the boundary of the granular and cornified layers of human epidermis. flg-klk5-3 4 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component colocalization colocalization 5 D-F Profilaggrin and KLK5 were co-localized in the vicinity of keratohyalin granules. (Keratohyalin is a protein structure found in granules in the stratum granulosum of the epidermis).