IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ VCP is essential for mitochondrial quality control by PINK1/Parkin and this function is impaired by VCP mutations. VCP is essential for mitochondrial quality control by PINK1/Parkin and this function is impaired by VCP mutations. Neuron (0896-6273) 2013 imex curation Kim N.C., Tresse E., Kolaitis R.M., Molliex A., Thomas R.E., Alami N.H., Wang B., Joshi A., Smith R.B., Ritson G.P., Winborn B.J., Moore J., Lee J.Y., Yao T.P., Pallanck L., Kundu M., Taylor J.P. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. email sent 21/3 jpaul.taylor@stjude.org 02-Apr-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kim N.C., Tresse E., Kolaitis R.M., Molliex A., Thomas R.E., Alami N.H., Wang B., Joshi A., Smith R.B., Ritson G.P., Winborn B.J., Moore J., Lee J.Y., Yao T.P., Pallanck L., Kundu M., Taylor J.P. Parkinsons - Interactions investigated in the context of Parkinsons disease email sent 21/3 Cells were transfected with YFP-Parkin. The authors have confirmed that FLAG-VCP is of Human origin. jpaul.taylor@stjude.org Accepted 2014-MAR-25 by PPORRAS Neuron (0896-6273) 2013 Only protein-protein interactions imex curation VCP is essential for mitochondrial quality control by PINK1/Parkin and this function is impaired by VCP mutations. VCP is essential for mitochondrial quality control by PINK1/Parkin and this function is impaired by VCP mutations. Neuron (0896-6273) 2013 imex curation Kim N.C., Tresse E., Kolaitis R.M., Molliex A., Thomas R.E., Alami N.H., Wang B., Joshi A., Smith R.B., Ritson G.P., Winborn B.J., Moore J., Lee J.Y., Yao T.P., Pallanck L., Kundu M., Taylor J.P. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. email sent 21/3 jpaul.taylor@stjude.org 02-Apr-2014: Contacted by IntAct-Help. Only protein-protein interactions drome-larval_brain Drosophila melanogaster larval brain larva_brain brain in insect larval stage of development anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kim N.C., Tresse E., Kolaitis R.M., Molliex A., Thomas R.E., Alami N.H., Wang B., Joshi A., Smith R.B., Ritson G.P., Winborn B.J., Moore J., Lee J.Y., Yao T.P., Pallanck L., Kundu M., Taylor J.P. Parkinsons - Interactions investigated in the context of Parkinsons disease Figure 6E interaction cannot be included due to lack of antibody specificity;however the text states that "that VCP interacts with Mfn2 in vitro but only after mitochondrial membrane depolarization". As the molecular weights of Mfn1 and 2 are very similar (84 and 86 kDa respectively) it is not clear how Mfn2 was distinguished from Mfn1. email sent 21/3 jpaul.taylor@stjude.org Accepted 2014-MAR-25 by PPORRAS Neuron (0896-6273) 2013 Only protein-protein interactions imex curation marf_drome Transmembrane GTPase Marf Marf dmfn Mitochondrial assembly regulatory factor Mitofusin CG3869 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAAYLNRTISMVTGQTGPADDDRHASSTDTVDKSGPGSPLSRFNSSLQQSGSTMAANLLPESRLYQSNDKSPLQIFVRAKKKINDIYGEIEEYVHETTTFINALHAEAEIVDKAERELFESYVYKVAAIREVLQRDHMKVAFFGRTSNGKSSVINAMLREKILPSGIGHTTNCFCQVEGSNGGEAYLMTEGSEEKLNVVNIKQLANALCQEKLCESSLVRIFWPRERCSLLRDDVVFVDSPGVDVSANLDDWIDNHCLNADVFVLVLNAESTMTRAEKQFFHTVSQKLSKPNIFILNNRWDASANEPECQESVKSQHTERCIDFLTKELKVSNEKEAAERVFFVSARETLQARIEEAKGNPPHMGAIAEGFQIRYFEFQDFERKFEECISQSAVKTKFQQHSSRGKSVSGDMKSMLDNIYERITIFRNLKQDQKNLLTERIQGTETQMMQVTREMKMKIHNMVEEVEEKVSKALNEEIWRLGVLIDEFNMPFHPERLVLNIYKKELNAHVESGLGSNLRARLSMALAMNVESAQTEMTDRMHALVPNEQLLATSTKMVVRTQPFEMLYSLNCQNLCADFQEDLEFKFSWGIAAMIQRFTGKVRERSKKGQPALVNRQSSIGHSVSTPTTTPVEATPVCLLPAPVVAGITPEQLSLISRFAVSSIGSQGTVGGLVVAGVMLKTIGWRVLVGVGALYGCIYLYERLSWTNSAKERTFKSQYVRHATKKLKMIVDLTSANCSHQVQQELSSTFARLCRTVDTATTDMNDELKTLDSQLNILEANQKQLKLLRNKANYIQNELDIFEHNYISPQ DIP protein Q7YU24 original sequence version: 58 8A3362BE1DD2A115 tera_drome Transitional endoplasmic reticulum ATPase TER94 TER94 VCP Valosin-containing protein homolog CG2331 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MADSKGEDLATAILKRKDRPNRLIVEEAQNDDNSVVSLSQAKMDELQLFRGDTVILKGKRRKETVCIVLSDDTCPDEKIRMNRVVRNNLCVHLSDVVSVQSCPDVKYGKRVRILPIDESTEGVTGNLFEIYLKPYFLEAYRPIHMGDNFIVRAAMRPIEFKVVLTDPEPYCIVAPETVIFCDGDPIKREEEEESLNAVGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPPRGILMYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNSPAIIFIDEIDAIAPKRDKTHGEVERRIVSQLLTLMDGMKKSSHLIVMAATNRPNSIDPALRRFGRFDREIDIGIPDATGRLEVLRIHTKNMKLHDDVDLEQIAAESHGHVGADLASLCSEAALQQIREKMDLIDLEDDKIDAEVLASLAVTMENFRYAMTKSSPSALRETVVEVPNTTWTDIGGLESVKKELQELVQYPVEHPDKFLKFGMQPSRGVLFYGPPGCGKTLLAKAIANECQANFISVKGPELLTMWFGESEANVRDIFDKARSAAPCVLFFDELDSIAKARGGNVGDAGGAADRVINQILTEMDGMGAKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDDKSREAILKANLRKSPLAKEVDLTYIAKVTQGFSGADLTEICQRACKLAIRQAIEAEIRREKERAENQNSAMDMDEDDPVPEITSAHFEEAMKFARRSVSDNDIRKYEMFAQTLQQSRGFGQNFRFPGQTGNTSGSGNNLPVNSPGDNGDDDLYS DIP protein Q7KN62 original sequence version: 92 ECF8F173B3B10B07 tera_human Transitional endoplasmic reticulum ATPase VCP 15S Mg(2+)-ATPase p97 subunit Valosin-containing protein protein protein human Homo sapiens Human MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG DIP protein P55072 original sequence version: 89 DIP protein P55072 original sequence version: 114 DIP protein P55072 original sequence version: 123 DIP protein P55072 original sequence version: 150 DIP protein P55072 original sequence version: 174 DIP protein P55072 original sequence version: 175 DIP protein P55072 original sequence version: 176 DIP protein P55072 original sequence version: 160 DIP protein P55072 original sequence version: 128 DIP protein P55072 original sequence version: 132 501B721D3A77BA8A mfn2_human Mitofusin-2 MFN2 CPRP1 KIAA0214 Transmembrane GTPase MFN2 protein protein human Homo sapiens Human MSLLFSRCNSIVTVKKNKRHMAEVNASPLKHFVTAKKKINGIFEQLGAYIQESATFLEDTYRNAELDPVTTEEQVLDVKGYLSKVRGISEVLARRHMKVAFFGRTSNGKSTVINAMLWDKVLPSGIGHTTNCFLRVEGTDGHEAFLLTEGSEEKRSAKTVNQLAHALHQDKQLHAGSLVSVMWPNSKCPLLKDDLVLMDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKHFFHKVSERLSRPNIFILNNRWDASASEPEYMEEVRRQHMERCTSFLVDELGVVDRSQAGDRIFFVSAKEVLNARIQKAQGMPEGGGALAEGFQVRMFEFQNFERRFEECISQSAVKTKFEQHTVRAKQIAEAVRLIMDSLHMAAREQQVYCEEMREERQDRLKFIDKQLELLAQDYKLRIKQITEEVERQVSTAMAEEIRRLSVLVDDYQMDFHPSPVVLKVYKNELHRHIEEGLGRNMSDRCSTAITNSLQTMQQDMIDGLKPLLPVSVRSQIDMLVPRQCFSLNYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALMGYNDQVQRPIPLTPANPSMPPLPQGSLTQEEFMVSMVTGLASLTSRTSMGILVVGGVVWKAVGWRLIALSFGLYGLLYVYERLTWTTKAKERAFKRQFVEHASEKLQLVISYTGSNCSHQVQQELSGTFAHLCQQVDVTRENLEQEIAAMNKKIEVLDSLQSKAKLLRNKAGWLDSELNMFTHQYLQPSR DIP protein O95140 original sequence version: 125 DIP protein O95140-1 original sequence version: 67 DIP protein O95140 original sequence version: 136 6F859D740152DFAD ter94-marf-1 2 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 6H vcp-mfn2 1 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey physical association physical association 6E VCP interacts with Mfn2, but only after mitochondrial membrane depolarization. CCCP- carbonyl cyanide m-chlorophenyl-hydrazone. A mitochondrial uncoupling agent, inhibitor of oxidative phosphorylation