IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The molecular basis of the Caskin1 and Mint1 interaction with CASK. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. (0022-2836) 2011 imex curation Stafford R.L., Ear J., Knight M.J., Bowie J.U. bowie@mbi.ucla.edu 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Stafford R.L., Ear J., Knight M.J., Bowie J.U. bowie@mbi.ucla.edu Accepted 2014-FEB-07 by PPORRAS J. Mol. Biol. (0022-2836) 2011 Only protein-protein interactions imex curation The molecular basis of the Caskin1 and Mint1 interaction with CASK. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. (0022-2836) 2011 imex curation Stafford R.L., Ear J., Knight M.J., Bowie J.U. bowie@mbi.ucla.edu 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro bn-page blue native page bn-page tag fluorescence tag visualisation by fluorescence tag fluorescence Stafford R.L., Ear J., Knight M.J., Bowie J.U. bowie@mbi.ucla.edu Accepted 2014-FEB-07 by PPORRAS J. Mol. Biol. (0022-2836) 2011 Only protein-protein interactions imex curation The molecular basis of the Caskin1 and Mint1 interaction with CASK. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. (0022-2836) 2011 imex curation Stafford R.L., Ear J., Knight M.J., Bowie J.U. bowie@mbi.ucla.edu 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Stafford R.L., Ear J., Knight M.J., Bowie J.U. bowie@mbi.ucla.edu Accepted 2014-FEB-07 by PPORRAS J. Mol. Biol. (0022-2836) 2011 Only protein-protein interactions imex curation apba1_human Amyloid-beta A4 precursor protein-binding family A member 1 APBA1 MINT1 X11 Neuron-specific X11 protein Neuronal Munc18-1-interacting protein 1 Adapter protein X11alpha protein protein human Homo sapiens Human MNHLEGSAEVEVTDEAAGGEVNESVEADLEHPEVEEEQQQPPQQQHYVGRHQRGRALEDLRAQLGQEEEERGECLARSASTESGFHNHTDTAEGDVIAAARDGYDAERAQDPEDESAYAVQYRPEAEEYTEQAEAEHAEATHRRALPNHLHFHSLEHEEAMNAAYSGYVYTHRLFHRGEDEPYSEPYADYGGLQEHVYEEIGDAPELDARDGLRLYEQERDEAAAYRQEALGARLHHYDERSDGESDSPEKEAEFAPYPRMDSYEQEEDIDQIVAEVKQSMSSQSLDKAAEDMPEAEQDLERPPTPAGGRPDSPGLQAPAGQQRAVGPAGGGEAGQRYSKEKRDAISLAIKDIKEAIEEVKTRTIRSPYTPDEPKEPIWVMRQDISPTRDCDDQRPMDGDSPSPGSSSPLGAESSSTSLHPSDPVEASTNKESRKSLASFPTYVEVPGPCDPEDLIDGIIFAANYLGSTQLLSDKTPSKNVRMMQAQEAVSRIKMAQKLAKSRKKAPEGESQPMTEVDLFISTQRIKVLNADTQETMMDHPLRTISYIADIGNIVVLMARRRMPRSNSQENVEASHPSQDGKRQYKMICHVFESEDAQLIAQSIGQAFSVAYQEFLRANGINPEDLSQKEYSDLLNTQDMYNDDLIHFSKSENCKDVFIEKQKGEILGVVIVESGWGSILPTVIIANMMHGGPAEKSGKLNIGDQIMSINGTSLVGLPLSTCQSIIKGLKNQSRVKLNIVRCPPVTTVLIRRPDLRYQLGFSVQNGIICSLMRGGIAERGGVRVGHRIIEINGQSVVATPHEKIVHILSNAVGEIHMKTMPAAMYRLLTAQEQPVYI 29C3620BBC89534B tiam1_human Rho guanine nucleotide exchange factor TIAM1 TIAM1 T-lymphoma invasion and metastasis-inducing protein 1 protein protein human Homo sapiens Human MGNAESQHVEHEFYGEKHASLGRKHTSRSLRLSHKTRRTRHASSGKVIHRNSEVSTRSSSTPSIPQSLAENGLEPFSQDGTLEDFGSPIWVDRVDMGLRPVSYTDSSVTPSVDSSIVLTAASVQSMPDTEESRLYGDDATYLAEGGRRQHSYTSNGPTFMETASFKKKRSKSADIWREDSLEFSLSDLSQEHLTSNEEILGSAEEKDCEEARGMETRASPRQLSTCQRANSLGDLYAQKNSGVTANGGPGSKFAGYCRNLVSDIPNLANHKMPPAAAEETPPYSNYNTLPCRKSHCLSEGATNPQISHSNSMQGRRAKTTQDVNAGEGSEFADSGIEGATTDTDLLSRRSNATNSSYSPTTGRAFVGSDSGSSSTGDAARQGVYENFRRELEMSTTNSESLEEAGSAHSDEQSSGTLSSPGQSDILLTAAQGTVRKAGALAVKNFLVHKKNKKVESATRRKWKHYWVSLKGCTLFFYESDGRSGIDHNSIPKHAVWVENSIVQAVPEHPKKDFVFCLSNSLGDAFLFQTTSQTELENWITAIHSACATAVARHHHKEDTLRLLKSEIKKLEQKIDMDEKMKKMGEMQLSSVTDSKKKKTILDQIFVWEQNLEQFQMDLFRFRCYLASLQGGELPNPKRLLAFASRPTKVAMGRLGIFSVSSFHALVAARTGETGVRRRTQAMSRSASKRRSRFSSLWGLDTTSKKKQGRPSINQVFGEGTEAVKKSLEGIFDDIVPDGKREKEVVLPNVHQHNPDCDIWVHEYFTPSWFCLPNNQPALTVVRPGDTARDTLELICKTHQLDHSAHYLRLKFLIENKMQLYVPQPEEDIYELLYKEIEICPKVTQSIHIEKSDTAADTYGFSLSSVEEDGIRRLYVNSVKETGLASKKGLKAGDEILEINNRAADALNSSMLKDFLSQPSLGLLVRTYPELEEGVELLESPPHRVDGPADLGESPLAFLTSNPGHSLCSEQGSSAETAPEETEGPDLESSDETDHSSKSTEQVAAFCRSLHEMNPSDQSPSPQDSTGPQLATMRQLSDADKLRKVICELLETERTYVKDLNCLMERYLKPLQKETFLTQDELDVLFGNLTEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQFKKVLFSLGGSFLYYADRFKLYSAFCASHTKVPKVLVKAKTDTAFKAFLDAQNPKQQHSSTLESYLIKPIQRILKYPLLLRELFALTDAESEEHYHLDVAIKTMNKVASHINEMQKIHEEFGAVFDQLIAEQTGEKKEVADLSMGDLLLHTTVIWLNPPASLGKWKKEPELAAFVFKTAVVLVYKDGSKQKKKLVGSHRLSIYEDWDPFRFRHMIPTEALQVRALASADAEANAVCEIVHVKSESEGRPERVFHLCCSSPESRKDFLKAVHSILRDKHRRQLLKTESLPSSQQYVPFGGKRLCALKGARPAMSRAVSAPSKSLGRRRRRLARNRFTIDSDAVSASSPEKESQQPPGGGDTDRWVEEQFDLAQYEEQDDIKETDILSDDDEFCESVKGASVDRDLQERLQATSISQRERGRKTLDSHASRMAQLKKQAALSGINGGLESASEEVIWVRREDFAPSRKLNTEI DIP protein Q13009 original sequence version: 132 DIP protein Q13009 original sequence version: 130 DIP protein Q13009 original sequence version: 119 0DAFBEE9A84B3452 cski1_human Caskin-1 CASKIN1 KIAA1306 CASK-interacting protein 1 protein protein human Homo sapiens Human MGKEQELVQAVKAEDVGTAQRLLQRPRPGKAKLLGSTKKINVNFQDPDGFSALHHAALNGNTELISLLLEAQAAVDIKDNKGMRPLHYAAWQGRKEPMKLVLKAGSAVNIPSDEGHIPLHLAAQHGHYDVSEMLLQHQSNPCMVDNSGKTPLDLACEFGRVGVVQLLLSSNMCAALLEPRPGDATDPNGTSPLHLAAKNGHIDIIRLLLQAGIDINRQTKSGTALHEAALCGKTEVVRLLLDSGINAHVRNTYSQTALDIVHQFTTSQASREIKQLLREASAALQVRATKDYCNNYDLTSLNVKAGDIITVLEQHPDGRWKGCIHDNRTGNDRVGYFPSSLGEAIVKRAGSRAGTEPSLPQGSSSSGPSAPPEEIWVLRKPFAGGDRSGSISGMAGGRGSGGHALHAGSEGVKLLATVLSQKSVSESGPGDSPAKPPEGSAGVARSQPPVAHAGQVYGEQPPKKLEPASEGKSSEAVSQWLTAFQLQLYAPNFISAGYDLPTISRMTPEDLTAIGVTKPGHRKKIAAEISGLSIPDWLPEHKPANLAVWLSMIGLAQYYKVLVDNGYENIDFITDITWEDLQEIGITKLGHQKKLMLAVRKLAELQKAEYAKYEGGPLRRKAPQSLEVMAIESPPPPEPTPADCQSPKMTTFQDSELSDELQAAMTGPAEVGPTTEKPSSHLPPTPRATTRQDSSLGGRARHMSSSQELLGDGPPGPSSPMSRSQEYLLDEGPAPGTPPREARPGRHGHSIKRASVPPVPGKPRQVLPPGTSHFTPPQTPTKTRPGSPQALGGPHGPAPATAKVKPTPQLLPPTERPMSPRSLPQSPTHRGFAYVLPQPVEGEVGPAAPGPAPPPVPTAVPTLCLPPEADAEPGRPKKRAHSLNRYAASDSEPERDELLVPAAAGPYATVQRRVGRSHSVRAPAGADKNVNRSQSFAVRPRKKGPPPPPPKRSSSALASANLADEPVPDAEPEDGLLGVRAQCRRASDLAGSVDTGSAGSVKSIAAMLELSSIGGGGRAARRPPEGHPTPRPASPEPGRVATVLASVKHKEAIGPGGEVVNRRRTLSGPVTGLLATARRGPGESADPGPFVEDGTGRQRPRGPSKGEAGVEGPPLAKVEASATLKRRIRAKQNQQENVKFILTESDTVKRRPKAKEREAGPEPPPPLSVYHNGTGTVRRRPASEQAGPPELPPPPPPAEPPPTDLAHLPPLPPPEGEARKPAKPPVSPKPVLTQPVPKLQGSPTPTSKKVPLPGPGSPEVKRAHGTPPPVSPKPPPPPTAPKPVKAVAGLPSGSAGPSPAPSPARQPPAALAKPPGTPPSLGASPAKPPSPGAPALHVPAKPPRAAAAAAAAAAAPPAPPEGASPGDSARQKLEETSACLAAALQAVEEKIRQEDAQGPRDSAAEKSTGSILDDIGSMFDDLADQLDAMLE DIP protein Q8WXD9 original sequence version: 76 CDF60E68B81E884A cskp_human Peripheral plasma membrane protein CASK CASK LIN2 Calcium/calmodulin-dependent serine protein kinase Protein lin-2 homolog protein protein human Homo sapiens Human MADDDVLFEDVYELCEVIGKGPFSVVRRCINRETGQQFAVKIVDVAKFTSSPGLSTEDLKREASICHMLKHPHIVELLETYSSDGMLYMVFEFMDGADLCFEIVKRADAGFVYSEAVASHYMRQILEALRYCHDNNIIHRDVKPHCVLLASKENSAPVKLGGFGVAIQLGESGLVAGGRVGTPHFMAPEVVKREPYGKPVDVWGCGVILFILLSGCLPFYGTKERLFEGIIKGKYKMNPRQWSHISESAKDLVRRMLMLDPAERITVYEALNHPWLKERDRYAYKIHLPETVEQLRKFNARRKLKGAVLAAVSSHKFNSFYGDPPEELPDFSEDPTSSGLLAAERAVSQVLDSLEEIHALTDCSEKDLDFLHSVFQDQHLHTLLDLYDKINTKSSPQIRNPPSDAVQRAKEVLEEISCYPENNDAKELKRILTQPHFMALLQTHDVVAHEVYSDEALRVTPPPTSPYLNGDSPESANGDMDMENVTRVRLVQFQKNTDEPMGITLKMNELNHCIVARIMHGGMIHRQGTLHVGDEIREINGISVANQTVEQLQKMLREMRGSITFKIVPSYRTQSSSCERDSPSTSRQSPANGHSSTNNSVSDLPSTTQPKGRQIYVRAQFEYDPAKDDLIPCKEAGIRFRVGDIIQIISKDDHNWWQGKLENSKNGTAGLIPSPELQEWRVACIAMEKTKQEQQASCTWFGKKKKQYKDKYLAKHNAVFDQLDLVTYEEVVKLPAFKRKTLVLLGAHGVGRRHIKNTLITKHPDRFAYPIPHTTRPPKKDEENGKNYYFVSHDQMMQDISNNEYLEYGSHEDAMYGTKLETIRKIHEQGLIAILDVEPQALKVLRTAEFAPFVVFIAAPTITPGLNEDESLQRLQKESDILQRTYAHYFDLTIINNEIDETIRHLEEAVELVCTAPQWVPVSWVY 6C02008CE52728BA caskin1-cask 1 7 unspecified role unspecified role prey prey ser/thr_dual-sp_kinase_dom sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobd Escherichia coli (strain B / BL21-DE3) 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mektr-tag n-terminus tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobd Escherichia coli (strain B / BL21-DE3) 13 10 direct interaction direct interaction Fig. 2b cask-caskin1-2 3 7 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ser/thr_dual-sp_kinase_dom sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecobd Escherichia coli (strain B / BL21-DE3) 6 unspecified role unspecified role neutral component neutral component c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q8WXD9:p.Ile375_Val377delinsAlaAlaAla mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain IWV AAA Thu Jul 07 12:17:55 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was ile375ala_trp376ala_val377ala Fri Jul 22 10:50:16 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ile375ala,trp376ala,val377ala' Wed May 02 16:51:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile_trp_val375-377ala_ala_ala' Tue Jun 30 16:32:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile375_Val377delinsAlaAlaAla' region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain Q8WXD9:p.Leu378_Lys380delinsAlaAlaAla mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain LRK AAA Thu Jul 07 12:17:57 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was leu378ala_arg379ala_lys380ala Fri Jul 22 11:02:36 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu378ala,arg379ala,lys380ala' Wed May 02 14:59:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu_arg_lys378-380ala_ala_ala' Tue Jun 30 17:23:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu378_Lys380delinsAlaAlaAla' mektr-tag n-terminus tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobd Escherichia coli (strain B / BL21-DE3) 19 23 direct interaction direct interaction Fig. 3a, Fig. 3b cask-tiam1 2 5 unspecified role unspecified role neutral component neutral component n-terminus supercharged green fluorescent protein supercharged green fluorescent protein n-term range n-terminal range n-term range n-term range n-terminal range n-term range lig_camk_cask_1 motif sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli-ari814 Escherichia coli strain ARI814 ecoli-ari814 Escherichia coli strain ARI814 7 unspecified role unspecified role neutral component neutral component O14936:p.Val117Asp mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V D Wed May 02 18:50:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val117asp' Tue Jun 30 17:09:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val117Asp' ser/thr_dual-sp_kinase_dom sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O14936:p.Val117Gln mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V Q Wed May 02 15:41:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val117gln' Tue Jun 30 18:11:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val117Gln' ecobd Escherichia coli (strain B / BL21-DE3) 32 29 physical association physical association Fig. 4g, Fig. 6c apba1-cask 2 4 unspecified role unspecified role neutral component neutral component n-terminus supercharged green fluorescent protein supercharged green fluorescent protein n-term range n-terminal range n-term range n-term range n-terminal range n-term range lig_camk_cask_1 motif sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli-ari814 Escherichia coli strain ARI814 ecoli-ari814 Escherichia coli strain ARI814 7 unspecified role unspecified role neutral component neutral component ser/thr_dual-sp_kinase_dom sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain O14936:p.Val117Gln mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V Q Wed May 02 16:10:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val117gln' Tue Jun 30 17:11:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val117Gln' n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O14936:p.Val117Asp mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V D Wed May 02 16:49:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val117asp' Tue Jun 30 17:07:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val117Asp' ecobd Escherichia coli (strain B / BL21-DE3) 38 40 physical association physical association Fig. 4g, Fig. 6c cask-caskin1-3 2 6 unspecified role unspecified role neutral component neutral component n-terminus supercharged green fluorescent protein supercharged green fluorescent protein n-term range n-terminal range n-term range n-term range n-terminal range n-term range lig_camk_cask_1 motif required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q8WXD9:p.Ile375Ala mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A Wed May 02 19:06:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile375ala' Tue Jun 30 18:27:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile375Ala' Q8WXD9:p.Trp376Ala mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A Wed May 02 17:50:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp376ala' Tue Jun 30 17:13:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp376Ala' Q8WXD9:p.Val377Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A Wed May 02 15:30:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val377ala' Tue Jun 30 18:12:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val377Ala' Q8WXD9:p.Arg379Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A Wed May 02 20:03:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg379ala' Tue Jun 30 18:10:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg379Ala' ecoli-ari814 Escherichia coli strain ARI814 ecoli-ari814 Escherichia coli strain ARI814 7 unspecified role unspecified role neutral component neutral component O14936:p.Val117Gln mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V Q Wed May 02 17:17:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val117gln' Tue Jun 30 19:29:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val117Gln' O14936:p.Val117Asp mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V D Wed May 02 17:23:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val117asp' Tue Jun 30 17:02:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val117Asp' ser/thr_dual-sp_kinase_dom sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobd Escherichia coli (strain B / BL21-DE3) 55 47 physical association physical association Fig. 4a, Fig. 4b, Fig. 4c, Fig. 4g, Fig. 6c cask-caskin1-4 2 6 unspecified role unspecified role neutral component neutral component n-terminus supercharged green fluorescent protein supercharged green fluorescent protein n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecoli-ari814 Escherichia coli strain ARI814 ecoli-ari814 Escherichia coli strain ARI814 6 competitor competitor competitor neutral component neutral component lig_camk_cask_1 motif required to bind necessary binding region required to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain n-terminus supercharged green fluorescent protein supercharged green fluorescent protein n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli-ari814 Escherichia coli strain ARI814 ecoli-ari814 Escherichia coli strain ARI814 7 unspecified role unspecified role neutral component neutral component ser/thr_dual-sp_kinase_dom sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobd Escherichia coli (strain B / BL21-DE3) association association Fig. 4d, Fig. 4e, Fig. 4f The EEIWVLRK peptide of Caskin-1 (residues 373-380) has higher affinity for CASK (residues 1-310) then the Caskin-1 CID (CASK Interaction Domain) region (residues 375-471) but a similar affinity then an extended CID region of Caskin-1 (residues 373-471). The minimal EIWVLR peptide of Caskin-1 (residues 374-379) has a slightly reduced affinity for CASK (residues 1-310) compared to the extended CID region of Caskin-1 (residues 373-471).