UniProt UniProt http://www.uniprot.org Zipper-interacting protein kinase (ZIPK) modulates canonical Wnt/beta-catenin signaling through interaction with Nemo-like kinase and T-cell factor 4 (NLK/TCF4). Zipper-interacting protein kinase (ZIPK) modulates canonical Wnt/beta-catenin signaling through interaction with Nemo-like kinase and T-cell factor 4 (NLK/TCF4). J. Biol. Chem. (0021-9258) 2011 imex curation Togi S., Ikeda O., Kamitani S., Nakasuji M., Sekine Y., Muromoto R., Nanbo A., Oritani K., Kawai T., Akira S., Matsuda T. Results from a Y2H screen have not been included as species origin of proteins is unclear: results section states:- "....a yeast two-hybrid screen of a HeLa cDNA library"; yet the methods section states:- "mouse 11-day embryo MATCHMAKER cDNA library ..... were screened ". The authors did not reply to a request for clarification. The origins of transfected NLK and TCF7L2/TCF-4 have not been possible to trace, so interactions with tagged constructs of these molecules have not been entered here. tmatsuda@pharm.hokudai.ac.jp 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions human-sw480 Human SW480 adenocarcinoma cell line sw480 SW480- human primary colorectal adenocarcinoma cell line There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution and a C -> T mutation in codon 309 resulting in a Pro -> Ser substitution. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CCL-228&Template=cellBiology anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Togi S., Ikeda O., Kamitani S., Nakasuji M., Sekine Y., Muromoto R., Nanbo A., Oritani K., Kawai T., Akira S., Matsuda T. Results from a Y2H screen have not been included as species origin of proteins is unclear: results section states:- "....a yeast two-hybrid screen of a HeLa cDNA library"; yet the methods section states:- "mouse 11-day embryo MATCHMAKER cDNA library ..... were screened ". The authors did not reply to a request for clarification. The origins of transfected NLK and TCF7L2/TCF-4 have not been possible to trace, so interactions with tagged constructs of these molecules have not been entered here. tmatsuda@pharm.hokudai.ac.jp Accepted 2017-AUG-17 AT 09:16 BST AT 09:16 BST by MGT J. Biol. Chem. (0021-9258) 2011 imex curation Zipper-interacting protein kinase (ZIPK) modulates canonical Wnt/beta-catenin signaling through interaction with Nemo-like kinase and T-cell factor 4 (NLK/TCF4). Zipper-interacting protein kinase (ZIPK) modulates canonical Wnt/beta-catenin signaling through interaction with Nemo-like kinase and T-cell factor 4 (NLK/TCF4). J. Biol. Chem. (0021-9258) 2011 imex curation Togi S., Ikeda O., Kamitani S., Nakasuji M., Sekine Y., Muromoto R., Nanbo A., Oritani K., Kawai T., Akira S., Matsuda T. Results from a Y2H screen have not been included as species origin of proteins is unclear: results section states:- "....a yeast two-hybrid screen of a HeLa cDNA library"; yet the methods section states:- "mouse 11-day embryo MATCHMAKER cDNA library ..... were screened ". The authors did not reply to a request for clarification. The origins of transfected NLK and TCF7L2/TCF-4 have not been possible to trace, so interactions with tagged constructs of these molecules have not been entered here. tmatsuda@pharm.hokudai.ac.jp 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Togi S., Ikeda O., Kamitani S., Nakasuji M., Sekine Y., Muromoto R., Nanbo A., Oritani K., Kawai T., Akira S., Matsuda T. Results from a Y2H screen have not been included as species origin of proteins is unclear: results section states:- "....a yeast two-hybrid screen of a HeLa cDNA library"; yet the methods section states:- "mouse 11-day embryo MATCHMAKER cDNA library ..... were screened ". The authors did not reply to a request for clarification. The origins of transfected NLK and TCF7L2/TCF-4 have not been possible to trace, so interactions with tagged constructs of these molecules have not been entered here. tmatsuda@pharm.hokudai.ac.jp Accepted 2017-AUG-17 AT 09:16 BST AT 09:16 BST by MGT J. Biol. Chem. (0021-9258) 2011 imex curation nlk_human Serine/threonine-protein kinase NLK NLK LAK1 Nemo-like kinase Protein LAK1 protein protein human Homo sapiens Human MSLCGARANAKMMAAYNGGTSAAAAGHHHHHHHHLPHLPPPHLHHHHHPQHHLHPGSAAAVHPVQQHTSSAAAAAAAAAAAAAMLNPGQQQPYFPSPAPGQAPGPAAAAPAQVQAAAAATVKAHHHQHSHHPQQQLDIEPDRPIGYGAFGVVWSVTDPRDGKRVALKKMPNVFQNLVSCKRVFRELKMLCFFKHDNVLSALDILQPPHIDYFEEIYVVTELMQSDLHKIIVSPQPLSSDHVKVFLYQILRGLKYLHSAGILHRDIKPGNLLVNSNCVLKICDFGLARVEELDESRHMTQEVVTQYYRAPEILMGSRHYSNAIDIWSVGCIFAELLGRRILFQAQSPIQQLDLITDLLGTPSLEAMRTACEGAKAHILRGPHKQPSLPVLYTLSSQATHEAVHLLCRMLVFDPSKRISAKDALAHPYLDEGRLRYHTCMCKCCFSTSTGRVYTSDFEPVTNPKFDDTFEKNLSSVRQVKEIIHQFILEQQKGNRVPLCINPQSAAFKSFISSTVAQPSEMPPSPLVWE CE6AEBA7D8133989 dapk3_human Death-associated protein kinase 3 DAPK3 ZIPK DAP-like kinase Zipper-interacting protein kinase MYPT1 kinase protein protein human Homo sapiens Human MSTFRQEDVEDHYEMGEELGSGQFAIVRKCRQKGTGKEYAAKFIKKRRLSSSRRGVSREEIEREVNILREIRHPNIITLHDIFENKTDVVLILELVSGGELFDFLAEKESLTEDEATQFLKQILDGVHYLHSKRIAHFDLKPENIMLLDKNVPNPRIKLIDFGIAHKIEAGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGETKQETLTNISAVNYDFDEEYFSNTSELAKDFIRRLLVKDPKRRMTIAQSLEHSWIKAIRRRNVRGEDSGRKPERRRLKTTRLKEYTIKSHSSLPPNNSYADFERFSKVLEEAAAAEEGLRELQRSRRLCHEDVEALAAIYEEKEAWYREESDSLGQDLRRLRQELLKTEALKRQAQEEAKGALLGTSGLKRRFSRLENRYEALAKQVASEMRFVQDLVRALEQEKLQGVECGLR 56773008A6A61CF0 dapk3-nlk-2 2 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 1B nlk-dapk3 2 3 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association 1B nlk-dapk3-2 1 4 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection 3 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection physical association physical association 4A