UniProt UniProt http://www.uniprot.org Interaction of ataxin-3 with huntingtin-associated protein 1 through Josephin domain. Interaction of ataxin-3 with huntingtin-associated protein 1 through Josephin domain. Neuroreport (0959-4965) 2011 imex curation Takeshita Y., Fujinaga R., Kokubu K., Islam M.N., Jahan M.R., Yanai A., Kakizuka A., Shinoda K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Huntington's - Publications describing interactions involved in Huntington's disease shinoda@yamaguchi-u.ac.jp 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-neuro_2_a Mus musculus albino neuroblastoma cell line neuro 2 a (N2a) neuro_2_a Mouse Albino neuroblastoma neuro 2 a cell line anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Takeshita Y., Fujinaga R., Kokubu K., Islam M.N., Jahan M.R., Yanai A., Kakizuka A., Shinoda K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. shinoda@yamaguchi-u.ac.jp Accepted 2014-AUG-13 by ORCHARD Neuroreport (0959-4965) 2011 imex curation Interaction of ataxin-3 with huntingtin-associated protein 1 through Josephin domain. Interaction of ataxin-3 with huntingtin-associated protein 1 through Josephin domain. Neuroreport (0959-4965) 2011 imex curation Takeshita Y., Fujinaga R., Kokubu K., Islam M.N., Jahan M.R., Yanai A., Kakizuka A., Shinoda K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Huntington's - Publications describing interactions involved in Huntington's disease shinoda@yamaguchi-u.ac.jp 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-neuro_2_a Mus musculus albino neuroblastoma cell line neuro 2 a (N2a) neuro_2_a Mouse Albino neuroblastoma neuro 2 a cell line fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Takeshita Y., Fujinaga R., Kokubu K., Islam M.N., Jahan M.R., Yanai A., Kakizuka A., Shinoda K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. shinoda@yamaguchi-u.ac.jp Accepted 2014-AUG-13 by ORCHARD Neuroreport (0959-4965) 2011 imex curation hap1_human Huntingtin-associated protein 1 Neuroan 1 HAP1 HLP1 HAP2 protein protein human Homo sapiens Human MRPKRLGRCCAGSRLGPGDPAALTCAPSPSASPAPEPSAQPQARGTGQRVGSRATSGSQFLSEARTGARPASEAGAKAGARRPSAFSAIQGDVRSMPDNSDAPWTRFVFQGPFGSRATGRGTGKAAGIWKTPAAYVGRRPGVSGPERAAFIRELEEALCPNLPPPVKKITQEDVKVMLYLLEELLPPVWESVTYGMVLQRERDLNTAARIGQSLVKQNSVLMEENSKLEALLGSAKEEILYLRHQVNLRDELLQLYSDSDEEDEDEEEEEEEKEAEEEQEEEEAEEDLQCAHPCDAPKLISQEALLHQHHCPQLEALQEKLRLLEEENHQLREEASQLDTLEDEEQMLILECVEQFSEASQQMAELSEVLVLRLENYERQQQEVARLQAQVLKLQQRCRMYGAETEKLQKQLASEKEIQMQLQEESVWVGSQLQDLREKYMDCGGMLIEMQEEVKTLRQQPPVSTGSATHYPYSVPLETLPGFQETLAEELRTSLRRMISDPVYFMERNYEMPRGDTSSLRYDFRYSEDREQVRGFEAEEGLMLAADIMRGEDFTPAEEFVPQEELGAAKKVPAEEGVMEEAELVSEETEGWEEVELELDEATRMNVVTSALEASGLGPSHLDMNYVLQQLANWQDAHYRRQLRWKMLQKGECPHGALPAASRTSCRSSCR 10971B807941B4EE p54252-1 Ataxin-3 ATXN3 ATX3 MJD MJD1 SCA3 Machado-Joseph disease protein 1 Spinocerebellar ataxia type 3 protein MJD1a protein protein human Homo sapiens Human MESIFHEKQEGSLCAQHCLNNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNMDDSGFFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEHWFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDLPDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDGSGMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMTQTSGTNLTSEELRKRREAYFEKQQQKQQQQQQQQQQGDLSGQSSHPCERPATSSGALGSDLGKACSPFIMFATFTLYLTYELHVIFALHYSSFPL Variant in position: 349:YELHVIFALHYSSFPL->Missing (in short isoform 1, due to a stop-gain single nucleotide polymorphism, has reduced half-life due to increased proteasomal degradation, has reduced solubility and increased tendency to form aggregates, increased localization to the nucleus). 4B2477EB67C30EFF atxn3-hap1 2 3 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection 4 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P54252-1:p.Gln296[24] q27 poly-glutamine extension mutation mutation range range range range QQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQQQQQ Wed Jun 13 19:04:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.gln296[24]' Tue Jun 30 17:51:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln296[24]' colocalization colocalization 1i atxn3-hap1-2 1 4 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection josephin sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 3 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection physical association physical association 2o atxn3-hap1-3 2 3 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection 4 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection josephin sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 2n atxn3-hap1-4 2 3 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection 4 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P54252-1:p.Gln296[76] q79 poly-glutamine extension mutation mutation range range range range QQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ Wed Jun 13 18:34:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.gln296[76]' Tue Jun 30 17:17:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln296[76]' colocalization colocalization 3f atxn3-hap1-5 1 4 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P54252-1:p.Gln296[76] q79 poly-glutamine extension mutation mutation range range range range QQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ Wed Jun 13 18:36:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.gln296[76]' Tue Jun 30 17:32:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln296[76]' 3 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection physical association physical association 3h atxn3-hap1-6 1 3 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection 4 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P54252-1:p.Gln296[24] q27 poly-glutamine extension mutation mutation range range range range QQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQQQQQ Wed Jun 13 17:37:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.gln296[24]' Tue Jun 30 16:25:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln296[24]' physical association physical association 2o and 3h