IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ CCAAT/enhancer-binding protein β and NF-κB mediate high level expression of chemokine genes CCL3 and CCL4 by human chondrocytes in response to IL-1β. CCAAT/enhancer-binding protein β and NF-κB mediate high level expression of chemokine genes CCL3 and CCL4 by human chondrocytes in response to IL-1β. J. Biol. Chem. (0021-9258) 2010 imex curation Zhang Z., Bryan J.L., DeLassus E., Chang L.W., Liao W., Sandell L.J. IBD - Inflammatory bowel disease sandelll@wustl.edu 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro emsa supershift electrophoretic mobility supershift assay emsa supershift autoradiography autoradiography Zhang Z., Bryan J.L., DeLassus E., Chang L.W., Liao W., Sandell L.J. IBD - Inflammatory bowel disease sandelll@wustl.edu Accepted 2014-NOV-17 by MGT J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation CCAAT/enhancer-binding protein β and NF-κB mediate high level expression of chemokine genes CCL3 and CCL4 by human chondrocytes in response to IL-1β. CCAAT/enhancer-binding protein β and NF-κB mediate high level expression of chemokine genes CCL3 and CCL4 by human chondrocytes in response to IL-1β. J. Biol. Chem. (0021-9258) 2010 imex curation Zhang Z., Bryan J.L., DeLassus E., Chang L.W., Liao W., Sandell L.J. IBD - Inflammatory bowel disease sandelll@wustl.edu 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions human-tc28a2 Homo sapiens T/C-28a2 chondrocyte cell line tc-28a2 Human chondrocyte cell line T/C-28a2 ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Zhang Z., Bryan J.L., DeLassus E., Chang L.W., Liao W., Sandell L.J. IBD - Inflammatory bowel disease sandelll@wustl.edu Accepted 2014-NOV-17 by MGT J. Biol. Chem. (0021-9258) 2010 Only protein-protein interactions imex curation ccl4_human_gene C-C motif chemokine 4 (CCL4) human gene gene gene human Homo sapiens Human ccl3_human_cebpb-site_dna-probe Human CCL3 gene promoter CEBPB binding site DNA probe dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid human Homo sapiens Human CCTTGGGTTGACATATACCCCTCT cebpb_human CCAAT/enhancer-binding protein beta CEBPB TCF5 PP9092 Liver activator protein Nuclear factor NF-IL6 Transcription factor 5 Liver-enriched inhibitory protein protein protein human Homo sapiens Human MQRLVAWDPACLPLPPPPPAFKSMEVANFYYEADCLAAAYGGKAAPAAPPAARPGPRPPAGELGSIGDHERAIDFSPYLEPLGAPQAPAPATATDTFEAAPPAPAPAPASSGQHHDFLSDLFSDDYGGKNCKKPAEYGYVSLGRLGAAKGALHPGCFAPLHPPPPPPPPPAELKAEPGFEPADCKRKEEAGAPGGGAGMAAGFPYALRAYLGYQAVPSGSSGSLSTSSSSSPPGTPSPADAKAPPTACYAGAAPAPSQVKSKAKKTVDKHSDEYKIRRERNNIAVRKSRDKAKMRNLETQHKVLELTAENERLQKKVEQLSRELSTLRNLFKQLPEPLLASSGHC DIP protein P17676 original sequence version: 102 9B725BD6CCAA771D ccl4_human_cebpb-site_dna-probe Human CCL4 gene promoter CEBPB binding site DNA probe dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid human Homo sapiens Human CTTTCCTCTATGATGACATCGCCCT ccl3_human_gene C-C motif chemokine 3 (CCL3) human gene CCL3 G0S19-1 MIP1A SCYA3 gene gene human Homo sapiens Human cebpb-ccl3-1 2 7 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait association association Fig. 5a, 5b, 5c, 5e Interleukin-1 beta (IL-1beta) P17676 increases expression at RNA level of ENSG00000277632 cebpb-ccl4-1 2 3 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait association association Fig. 5a, 5b, 5d Interleukin-1 beta (IL-1beta) P17676 increases expression at RNA level of ENSG0000275302 ccl3-cebpb-1 1 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role prey prey human-tc28a2 Homo sapiens T/C-28a2 chondrocyte cell line tc-28a2 Human chondrocyte cell line T/C-28a2 physical association physical association Fig. 4c ccl4-cebpb-1 1 5 unspecified role unspecified role prey prey human-tc28a2 Homo sapiens T/C-28a2 chondrocyte cell line tc-28a2 Human chondrocyte cell line T/C-28a2 6 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Fig. 4c