IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Andersen's syndrome mutation effects on the structure and assembly of the cytoplasmic domains of Kir2.1. Andersen's syndrome mutation effects on the structure and assembly of the cytoplasmic domains of Kir2.1. Biochemistry (0006-2960) 2006 imex curation Pegan S., Arrabit C., Slesinger P.A., Choe S. choe@salk.edu 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Pegan S., Arrabit C., Slesinger P.A., Choe S. choe@salk.edu Accepted 2014-MAY-20 by MGT Biochemistry (0006-2960) 2006 Only protein-protein interactions imex curation Andersen's syndrome mutation effects on the structure and assembly of the cytoplasmic domains of Kir2.1. Andersen's syndrome mutation effects on the structure and assembly of the cytoplasmic domains of Kir2.1. Biochemistry (0006-2960) 2006 imex curation Pegan S., Arrabit C., Slesinger P.A., Choe S. choe@salk.edu 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Pegan S., Arrabit C., Slesinger P.A., Choe S. choe@salk.edu Accepted 2014-MAY-20 by MGT Biochemistry (0006-2960) 2006 Only protein-protein interactions imex curation kcnj2_mouse Inward rectifier potassium channel 2 Potassium channel, inwardly rectifying subfamily J member 2 Inward rectifier K(+) channel Kir2.1 Kcnj2 Irk1 protein protein mouse Mus musculus Mouse MGSVRTNRYSIVSSEEDGMKLATMAVANGFGNGKSKVHTRQQCRSRFVKKDGHCNVQFINVGEKGQRYLADIFTTCVDIRWRWMLVIFCLAFVLSWLFFGCVFWLIALLHGDLDTSKVSKACVSEVNSFTAAFLFSIETQTTIGYGFRCVTDECPIAVFMVVFQSIVGCIIDAFIIGAVMAKMAKPKKRNETLVFSHNAVIAMRDGKLCLMWRVGNLRKSHLVEAHVRAQLLKSRITSEGEYIPLDQIDINVGFDSGIDRIFLVSPITIVHEIDEDSPLYDLSKQDIDNADFEIVVILEGMVEATAMTTQCRSSYLANEILWGHRYEPVLFEEKHYYKVDYSRFHKTYEVPNTPLCSARDLAEKKYILSNANSFCYENEVALTSKEEEEDSENGVPESTSTDSPPGIDLHNQASVPLEPRPLRRESEI 5B4219F979BBA41C kcnj2-1 2 3 unspecified role unspecified role neutral component neutral component cytoplasmic_domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P35561:p.[Arg218Gln;Thr309Lys] mutation mutation certain certain sequence position certain certain certain sequence position certain R Q certain certain sequence position certain certain certain sequence position certain T K Wed May 02 20:28:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg218gln,thr309lys' Tue Jun 30 16:08:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg218Gln;Thr309Lys]' ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 R = 0.19, Rwork = 0.18, Rfree = 0.23 2.02 A The structure of a slightly longer construct (44-64 & 189-428) is depicted in Fig. 2a and 4d but has not been submitted to the PDB. kcnj2-2 1 3 unspecified role unspecified role neutral component neutral component cytoplasmic_domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 2b Mutations Glu303Lys, Gly300Val, Arg218Gln/Thr309Lys and Arg218Gln/Thr309Arg all cause slight shifts in the absorbance profile towards a larger molecular weight but less than an even number of change in stoichiometry. kcnj2-1 1 3 unspecified role unspecified role neutral component neutral component P35561:p.Arg218Gln mutation mutation certain certain sequence position certain certain certain sequence position certain R Q Wed May 02 14:41:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg218gln' Tue Jun 30 18:56:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg218Gln' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cytoplasmic_domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role neutral component neutral component P35561:p.Arg218Gln mutation mutation certain certain sequence position certain certain certain sequence position certain R Q Wed May 02 14:58:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg218gln' Tue Jun 30 20:15:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg218Gln' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cytoplasmic_domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 2b Arg218Gln mutant causes aggregations.