UniProt UniProt http://www.uniprot.org Physical and functional interactions between STAT3 and ZIP kinase. Physical and functional interactions between STAT3 and ZIP kinase. Int. Immunol. (0953-8178) 2005 imex curation Sato N., Kawai T., Sugiyama K., Muromoto R., Imoto S., Sekine Y., Ishida M., Akira S., Matsuda T. Origin of STAT3 according pmid 10458605 (publication Ref. 14). Interaction with transfected Myc-tagged DAPK kinase domain (anti-tag CoIP, fig 1F) not included as protein cannot be mapped. tmatsuda@pharm.hokudai.ac.jp 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Sato N., Kawai T., Sugiyama K., Muromoto R., Imoto S., Sekine Y., Ishida M., Akira S., Matsuda T. Origin of STAT3 according pmid 10458605 (publication Ref. 14). Interaction with transfected Myc-tagged DAPK kinase domain (anti-tag CoIP, fig 1F) not included as protein cannot be mapped. tmatsuda@pharm.hokudai.ac.jp Accepted 2014-AUG-20 by ORCHARD Int. Immunol. (0953-8178) 2005 imex curation Physical and functional interactions between STAT3 and ZIP kinase. Physical and functional interactions between STAT3 and ZIP kinase. Int. Immunol. (0953-8178) 2005 imex curation Sato N., Kawai T., Sugiyama K., Muromoto R., Imoto S., Sekine Y., Ishida M., Akira S., Matsuda T. Origin of STAT3 according pmid 10458605 (publication Ref. 14). Interaction with transfected Myc-tagged DAPK kinase domain (anti-tag CoIP, fig 1F) not included as protein cannot be mapped. tmatsuda@pharm.hokudai.ac.jp 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Sato N., Kawai T., Sugiyama K., Muromoto R., Imoto S., Sekine Y., Ishida M., Akira S., Matsuda T. Origin of STAT3 according pmid 10458605 (publication Ref. 14). Interaction with transfected Myc-tagged DAPK kinase domain (anti-tag CoIP, fig 1F) not included as protein cannot be mapped. tmatsuda@pharm.hokudai.ac.jp Accepted 2014-AUG-20 by ORCHARD Int. Immunol. (0953-8178) 2005 imex curation Physical and functional interactions between STAT3 and ZIP kinase. Physical and functional interactions between STAT3 and ZIP kinase. Int. Immunol. (0953-8178) 2005 imex curation Sato N., Kawai T., Sugiyama K., Muromoto R., Imoto S., Sekine Y., Ishida M., Akira S., Matsuda T. Origin of STAT3 according pmid 10458605 (publication Ref. 14). Interaction with transfected Myc-tagged DAPK kinase domain (anti-tag CoIP, fig 1F) not included as protein cannot be mapped. tmatsuda@pharm.hokudai.ac.jp 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-skw6.4 Human B-lymphoblastoid EBV-transformed cell line skw6.4 Human B-lymphoblastoid EBV-transformed cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sato N., Kawai T., Sugiyama K., Muromoto R., Imoto S., Sekine Y., Ishida M., Akira S., Matsuda T. Origin of STAT3 according pmid 10458605 (publication Ref. 14). Interaction with transfected Myc-tagged DAPK kinase domain (anti-tag CoIP, fig 1F) not included as protein cannot be mapped. tmatsuda@pharm.hokudai.ac.jp Accepted 2014-AUG-20 by ORCHARD Int. Immunol. (0953-8178) 2005 imex curation Physical and functional interactions between STAT3 and ZIP kinase. Physical and functional interactions between STAT3 and ZIP kinase. Int. Immunol. (0953-8178) 2005 imex curation Sato N., Kawai T., Sugiyama K., Muromoto R., Imoto S., Sekine Y., Ishida M., Akira S., Matsuda T. Origin of STAT3 according pmid 10458605 (publication Ref. 14). Interaction with transfected Myc-tagged DAPK kinase domain (anti-tag CoIP, fig 1F) not included as protein cannot be mapped. tmatsuda@pharm.hokudai.ac.jp 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Sato N., Kawai T., Sugiyama K., Muromoto R., Imoto S., Sekine Y., Ishida M., Akira S., Matsuda T. Origin of STAT3 according pmid 10458605 (publication Ref. 14). Interaction with transfected Myc-tagged DAPK kinase domain (anti-tag CoIP, fig 1F) not included as protein cannot be mapped. tmatsuda@pharm.hokudai.ac.jp Accepted 2014-AUG-20 by ORCHARD mouse 11-day embryo cDNA Int. Immunol. (0953-8178) 2005 imex curation Physical and functional interactions between STAT3 and ZIP kinase. Physical and functional interactions between STAT3 and ZIP kinase. Int. Immunol. (0953-8178) 2005 imex curation Sato N., Kawai T., Sugiyama K., Muromoto R., Imoto S., Sekine Y., Ishida M., Akira S., Matsuda T. Origin of STAT3 according pmid 10458605 (publication Ref. 14). Interaction with transfected Myc-tagged DAPK kinase domain (anti-tag CoIP, fig 1F) not included as protein cannot be mapped. tmatsuda@pharm.hokudai.ac.jp 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Sato N., Kawai T., Sugiyama K., Muromoto R., Imoto S., Sekine Y., Ishida M., Akira S., Matsuda T. Origin of STAT3 according pmid 10458605 (publication Ref. 14). Interaction with transfected Myc-tagged DAPK kinase domain (anti-tag CoIP, fig 1F) not included as protein cannot be mapped. tmatsuda@pharm.hokudai.ac.jp Accepted 2014-AUG-20 by ORCHARD Int. Immunol. (0953-8178) 2005 imex curation Physical and functional interactions between STAT3 and ZIP kinase. Physical and functional interactions between STAT3 and ZIP kinase. Int. Immunol. (0953-8178) 2005 imex curation Sato N., Kawai T., Sugiyama K., Muromoto R., Imoto S., Sekine Y., Ishida M., Akira S., Matsuda T. Origin of STAT3 according pmid 10458605 (publication Ref. 14). Interaction with transfected Myc-tagged DAPK kinase domain (anti-tag CoIP, fig 1F) not included as protein cannot be mapped. tmatsuda@pharm.hokudai.ac.jp 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Sato N., Kawai T., Sugiyama K., Muromoto R., Imoto S., Sekine Y., Ishida M., Akira S., Matsuda T. Origin of STAT3 according pmid 10458605 (publication Ref. 14). Interaction with transfected Myc-tagged DAPK kinase domain (anti-tag CoIP, fig 1F) not included as protein cannot be mapped. tmatsuda@pharm.hokudai.ac.jp Accepted 2014-AUG-20 by ORCHARD Int. Immunol. (0953-8178) 2005 imex curation Physical and functional interactions between STAT3 and ZIP kinase. Physical and functional interactions between STAT3 and ZIP kinase. Int. Immunol. (0953-8178) 2005 imex curation Sato N., Kawai T., Sugiyama K., Muromoto R., Imoto S., Sekine Y., Ishida M., Akira S., Matsuda T. Origin of STAT3 according pmid 10458605 (publication Ref. 14). Interaction with transfected Myc-tagged DAPK kinase domain (anti-tag CoIP, fig 1F) not included as protein cannot be mapped. tmatsuda@pharm.hokudai.ac.jp 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Sato N., Kawai T., Sugiyama K., Muromoto R., Imoto S., Sekine Y., Ishida M., Akira S., Matsuda T. Origin of STAT3 according pmid 10458605 (publication Ref. 14). Interaction with transfected Myc-tagged DAPK kinase domain (anti-tag CoIP, fig 1F) not included as protein cannot be mapped. tmatsuda@pharm.hokudai.ac.jp Accepted 2014-AUG-20 by ORCHARD mouse 11-day embryo cDNA Int. Immunol. (0953-8178) 2005 imex curation dapk3_human Death-associated protein kinase 3 DAPK3 ZIPK DAP-like kinase Zipper-interacting protein kinase MYPT1 kinase protein protein human Homo sapiens Human MSTFRQEDVEDHYEMGEELGSGQFAIVRKCRQKGTGKEYAAKFIKKRRLSSSRRGVSREEIEREVNILREIRHPNIITLHDIFENKTDVVLILELVSGGELFDFLAEKESLTEDEATQFLKQILDGVHYLHSKRIAHFDLKPENIMLLDKNVPNPRIKLIDFGIAHKIEAGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGETKQETLTNISAVNYDFDEEYFSNTSELAKDFIRRLLVKDPKRRMTIAQSLEHSWIKAIRRRNVRGEDSGRKPERRRLKTTRLKEYTIKSHSSLPPNNSYADFERFSKVLEEAAAAEEGLRELQRSRRLCHEDVEALAAIYEEKEAWYREESDSLGQDLRRLRQELLKTEALKRQAQEEAKGALLGTSGLKRRFSRLENRYEALAKQVASEMRFVQDLVRALEQEKLQGVECGLR 56773008A6A61CF0 stat3_mouse Signal transducer and activator of transcription 3 Acute-phase response factor Stat3 Aprf protein protein mouse Mus musculus Mouse MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMDLTSECATSPM DIP protein P42227 original sequence version: 169 DIP protein P42227 original sequence version: 162 DIP protein P42227 original sequence version: 164 DIP protein P42227 original sequence version: 141 6C00626711C8012D dapk3_mouse Death-associated protein kinase 3 Dapk3 Zipk DAP-like kinase ZIP-kinase MYPT1 kinase protein protein mouse Mus musculus Mouse MSTFRQEDVEDHYEMGEELGSGQFAIVRKCQQKGTGMEYAAKFIKKRRLPSSRRGVSREEIEREVSILREIRHPNIITLHDVFENKTDVVLILELVSGGELFDFLAEKESLTEDEATQFLKQILDGVHYLHSKRIAHFDLKPENIMLLDKHAASPRIKLIDFGIAHRIEAGSEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGETKQETLTNISAVNYDFDEEYFSSTSELAKDFIRRLLVKDPKRRMTIAQSLEHSWIKVRRREDGARKPERRRLRAARLREYSLKSHSSMPRNTSYASFERFSRVLEDVAAAEQGLRELQRGRRQCRERVCALRAAAEQREARCRDGSAGLGRDLRRLRTELGRTEALRTRAQEEARAALLGAGGLKRRLCRLENRYDALAAQVAAEVQFVRDLVRALEQERLQAECGVR DA32EF3EB1F20EFC stat3_human Signal transducer and activator of transcription 3 Acute-phase response factor STAT3 APRF protein protein human Homo sapiens Human MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM 6C00632211C8012D DIP protein P40763 original sequence version: 157 DIP protein P40763-1 original sequence version: 147 DIP protein P40763-1 original sequence version: 135 DIP protein P40763-1 original sequence version: 157 DIP protein P40763 original sequence version: 165 DIP protein P40763 original sequence version: 182 DIP protein P40763 original sequence version: 135 6C00632211C8012D stat3-dapk3-1 4 10 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 14 16 physical association physical association no figure stat3-dapk3 7 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 19 21 physical association physical association 1A stat3-dapk3-2 1 9 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection 10 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1B stat3-dapk3-3 5 10 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1C dapk3-stat3 5 9 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain DAPK3/ZIPK kinase-negative mutant lys42ala (mutant full length and aa1-275 fragment) also interacted. physical association physical association 1D stat3-dapk3-4 3 11 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 1E IL-6 stimulation stat3-dapk3-5 5 9 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined DAPK3/ZIPK kinase-negative mutant lys42ala (mutant full length and aa1-275 fragment) also interacted. physical association physical association 1F dapk3-3 6 10 putative self putative self putative self putative self region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true 2B Ser/Thr-protein kinase inhibitor H7 O54784 inhibits O54784 dapk3-stat3-1 6 9 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain synthetic construct synthetic construct 10 enzyme enzyme neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 2B Ser/Thr-protein kinase inhibitor H7 O54784 increases activity of P42227 P42227 ser-727 O-phospho-L-serine CHEBI:82739 decreases activity of O54784 dapk3-stat3-2 2 9 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 5B LIF (Leukemia inhibitory factor) stimulation