UniProt UniProt http://www.uniprot.org Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D). Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D). J. Biol. Chem. (0021-9258) 2003 mimix curation Braybrooke J.P., Li J.L., Wu L., Caple F., Benson F.E., Hickson I.D. ian.hickson@cancer.org.uk 10-Mar-2014: Contacted by IntAct-Help. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Braybrooke J.P., Li J.L., Wu L., Caple F., Benson F.E., Hickson I.D. ian.hickson@cancer.org.uk Accepted 2014-FEB-20 by MGT J. Biol. Chem. (0021-9258) 2003 mimix curation Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D). Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D). J. Biol. Chem. (0021-9258) 2003 mimix curation Braybrooke J.P., Li J.L., Wu L., Caple F., Benson F.E., Hickson I.D. ian.hickson@cancer.org.uk 10-Mar-2014: Contacted by IntAct-Help. ecoli Escherichia coli (strain K12) pull down pull down anti tag western anti tag western blot anti tag western Braybrooke J.P., Li J.L., Wu L., Caple F., Benson F.E., Hickson I.D. ian.hickson@cancer.org.uk Accepted 2014-FEB-20 by MGT J. Biol. Chem. (0021-9258) 2003 mimix curation Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D). Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D). J. Biol. Chem. (0021-9258) 2003 mimix curation Braybrooke J.P., Li J.L., Wu L., Caple F., Benson F.E., Hickson I.D. ian.hickson@cancer.org.uk 10-Mar-2014: Contacted by IntAct-Help. ecoli Escherichia coli (strain K12) affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom weight by staining molecular weight estimation by staining weight by staining Braybrooke J.P., Li J.L., Wu L., Caple F., Benson F.E., Hickson I.D. ian.hickson@cancer.org.uk Accepted 2014-FEB-20 by MGT J. Biol. Chem. (0021-9258) 2003 mimix curation Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D). Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D). J. Biol. Chem. (0021-9258) 2003 mimix curation Braybrooke J.P., Li J.L., Wu L., Caple F., Benson F.E., Hickson I.D. ian.hickson@cancer.org.uk 10-Mar-2014: Contacted by IntAct-Help. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Braybrooke J.P., Li J.L., Wu L., Caple F., Benson F.E., Hickson I.D. ian.hickson@cancer.org.uk Accepted 2014-FEB-20 by MGT J. Biol. Chem. (0021-9258) 2003 mimix curation Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D). Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D). J. Biol. Chem. (0021-9258) 2003 mimix curation Braybrooke J.P., Li J.L., Wu L., Caple F., Benson F.E., Hickson I.D. ian.hickson@cancer.org.uk 10-Mar-2014: Contacted by IntAct-Help. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Braybrooke J.P., Li J.L., Wu L., Caple F., Benson F.E., Hickson I.D. ian.hickson@cancer.org.uk Accepted 2014-FEB-20 by MGT J. Biol. Chem. (0021-9258) 2003 mimix curation Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D). Functional interaction between the Bloom's syndrome helicase and the RAD51 paralog, RAD51L3 (RAD51D). J. Biol. Chem. (0021-9258) 2003 mimix curation Braybrooke J.P., Li J.L., Wu L., Caple F., Benson F.E., Hickson I.D. ian.hickson@cancer.org.uk 10-Mar-2014: Contacted by IntAct-Help. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Braybrooke J.P., Li J.L., Wu L., Caple F., Benson F.E., Hickson I.D. ian.hickson@cancer.org.uk Accepted 2014-FEB-20 by MGT J. Biol. Chem. (0021-9258) 2003 mimix curation xrcc2_human DNA repair protein XRCC2 XRCC2 X-ray repair cross-complementing protein 2 protein protein human Homo sapiens Human MCSAFHRAESGTELLARLEGRSSLKEIEPNLFADEDSPVHGDILEFHGPEGTGKTEMLYHLTARCILPKSEGGLEVEVLFIDTDYHFDMLRLVTILEHRLSQSSEEIIKYCLGRFFLVYCSSSTHLLLTLYSLESMFCSHPSLCLLILDSLSAFYWIDRVNGGESVNLQESTLRKCSQCLEKLVNDYRLVLFATTQTIMQKASSSSEEPSHASRRLCDVDIDYRPYLCKAWQQLVKHRMFFSKQDDSQSSNQFSLVSRCLKSNSLKKHFFIIGESGVEFC DIP protein O43543 original sequence version: 105 5656277E74C06074 blm_human Bloom syndrome protein RecQ protein-like 3 DNA helicase, RecQ-like type 2 BLM RECQ2 RECQL3 protein protein human Homo sapiens Human MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDNNVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTSETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTEEQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLEEAELHSTEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDVLSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS DIP protein P54132 original sequence version: 155 DIP protein P54132 original sequence version: 109 DIP protein P54132 original sequence version: 146 DIP protein P54132 original sequence version: 111 423DF5F381194E11 ra51d_human DNA repair protein RAD51 homolog 4 R51H3 RAD51-like protein 3 TRAD RAD51D RAD51L3 RAD51 homolog D protein protein human Homo sapiens Human MGVLRVGLCPGLTEEMIQLLRSHRIKTVVDLVSADLEEVAQKCGLSYKALVALRRVLLAQFSAFPVNGADLYEELKTSTAILSTGIGSLDKLLDAGLYTGEVTEIVGGPGSGKTQVCLCMAANVAHGLQQNVLYVDSNGGLTASRLLQLLQAKTQDEEEQAEALRRIQVVHAFDIFQMLDVLQELRGTVAQQVTGSSGTVKVVVVDSVTAVVSPLLGGQQREGLALMMQLARELKTLARDLGMAVVVTNHITRDRDSGRLKPALGRSWSFVPSTRILLDTIEGAGASGGRRMACLAKSSRQPTGFQEMVDIGTWGTSEQSATLQGDQT DIP protein O75771 original sequence version: 130 6038DA9356DF354A rad51d-blm 5 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association 1A and B rad51d-xrcc2-10 5 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 1A and B xrcc2-blm 6 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 1C rad51d-blm-1 6 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association 1C rad51d-blm-3 1 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait direct interaction direct interaction 2A rad51d-blm-4 4 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait direct interaction direct interaction 2B rad51d-xrcc2-11 4 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait direct interaction direct interaction 2B xrcc2-rad51d-3 2 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 3 xrcc2-rad51d-4 3 7 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 3