IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Visualization of the domain structure of an L-type Ca2+ channel using electron cryo-microscopy. Visualization of the domain structure of an L-type Ca2+ channel using electron cryo-microscopy. J. Mol. Biol. (0022-2836) 2003 imex curation Wolf M., Eberhart A., Glossmann H., Striessnig J., Grigorieff N. Purification of the DHPR receptor is achieved with a well-established chromatographic method used in many other previous studies. For this reason, although some of the components of the purified receptor are only identified by MW, (as there are no specific antibodies against them) I accept this method of identification as valid. niko@brandeis.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Wolf M., Eberhart A., Glossmann H., Striessnig J., Grigorieff N. Purification of the DHPR receptor is achieved with a well-established chromatographic method used in many other previous studies. For this reason, although some of the components of the purified receptor are only identified by MW, (as there are no specific antibodies against them) I accept this method of identification as valid. Purification of the DHPR receptor is achieved with a well-established chromatographic method used in many other previous studies. For this reason, although some of the components of the purified receptor are only identified by MW, (as there are no specific antibodies against them) I accept this method of identification as valid. niko@brandeis.edu Accepted 2014-AUG-19 by ORCHARD J. Mol. Biol. (0022-2836) 2003 imex curation Visualization of the domain structure of an L-type Ca2+ channel using electron cryo-microscopy. Visualization of the domain structure of an L-type Ca2+ channel using electron cryo-microscopy. J. Mol. Biol. (0022-2836) 2003 imex curation Wolf M., Eberhart A., Glossmann H., Striessnig J., Grigorieff N. Purification of the DHPR receptor is achieved with a well-established chromatographic method used in many other previous studies. For this reason, although some of the components of the purified receptor are only identified by MW, (as there are no specific antibodies against them) I accept this method of identification as valid. niko@brandeis.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions rabbit-skeletal_muscle Oryctolagus cuniculus skeletal_muscle Skeletal muscle affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom weight silver stain molecular weight estimation by silver staining weight silver stain Wolf M., Eberhart A., Glossmann H., Striessnig J., Grigorieff N. Complex purified from rabbit skeletal muscle microsomes by a series of chromatographic/density gradient centrifugation steps. Purification of the DHPR receptor is achieved with a well-established chromatographic method used in many other previous studies. For this reason, although some of the components of the purified receptor are only identified by MW, (as there are no specific antibodies against them) I accept this method of identification as valid. Purification of the DHPR receptor is achieved with a well-established chromatographic method used in many other previous studies. For this reason, although some of the components of the purified receptor are only identified by MW, (as there are no specific antibodies against them) I accept this method of identification as valid. niko@brandeis.edu Accepted 2014-AUG-19 by ORCHARD J. Mol. Biol. (0022-2836) 2003 imex curation p13806-pro_0000005007 Voltage-dependent calcium channel subunit alpha-2-1 CACNA2D1 CACNL2A CCHL2A Voltage-gated calcium channel subunit alpha-2/delta-1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit EPFPSAVTIKSWVDKMQEDLVTLAKTASGVHQLVDIYEKYQDLYTVEPNNARQLVEIAARDIEKLLSNRSKALVRLALEAEKVQAAHQWREDFASNEVVYYNAKDDLDPEKNDSEPGSQRIKPVFIDDANFRRQVSYQHAAVHIPTDIYEGSTIVLNELNWTSALDDVFKKNREEDPSLLWQVFGSATGLARYYPASPWVDNSRTPNKIDLYDVRRRPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEMLETLSDDDFVNVASFNSNAQDVSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVSRANCNKIIMLFTDGGEERAQEIFAKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIRINTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNITGQFENKTNLKNQLILGVMGVDVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKPIGVGIPTINLRKRRPNVQNPKSQEPVTLDFLDAELENDIKVEIRNKMIDGESGEKTFRTLVKSQDERYIDKGNRTYTWTPVNGTDYSSLALVLPTYSFYYIKAKIEETITQARYSETLKPDNFEESGYTFLAPRDYCSDLKPSDNNTEFLLNFNEFIDRKTPNNPSCNTDLINRVLLDAGFTNELVQNYWSKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYFNKSGPGAYESGIMVSKAVEIYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCDCKRNSDVMDCVILDDGGFLLMANHDDYTNQIGRFFGEIDPSLMRHLVNISVYAFNKSYDYQSVCEPGAAPKQGAGHRSAYVPSIADILQIGWWATAAAWSILQQFLLSLTFPRLLEA 27 960 1B2CFD6B5C5A6006 cac1s_rabit Voltage-dependent L-type calcium channel subunit alpha-1S CACNA1S CACH1 CACNL1A3 Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle Voltage-gated calcium channel subunit alpha Cav1.1 Dihydropyridine receptor alpha-1S subunit protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MEPSSPQDEGLRKKQPKKPLPEVLPRPPRALFCLTLQNPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNSLNLGLEKLEYFFLTVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTAILEQVNVIQSNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYYIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCITMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWITQGEVMDVEDLREGKLSLEEGGSDTESLYEIEGLNKIIQFIRHWRQWNRVFRWKCHDLVKSRVFYWLVILIVALNTLSIASEHHNQPLWLTHLQDIANRVLLSLFTIEMLLKMYGLGLRQYFMSIFNRFDCFVVCSGILELLLVESGAMTPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIIIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWNSVMYNGIMAYGGPSYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSRGLPDKTEEEKSVMAKKLEQKPKGEGIPTTAKLKVDEFESNVNEVKDPYPSADFPGDDEEDEPEIPVSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKVRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRAESVRNQILGYFDIAFTSVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSTISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFFSCNDLSKMTEEECRGYYYVYKDGDPTQMELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYRAIDSNEEDMGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQHYHQSEEMNHISDILNVAFTIIFTLEMILKLLAFKARGYFGDPWNVFDFLIVIGSIIDVILSEIDTFLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLIKLLSRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPGEEYTCGTNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKDTVQIQAGLRTIEEEAAPEIRRTISGDLTAEEELERAMVEAAMEERIFRRTGGLFGQVDTFLERTNSLPPVMANQRPLQFAEIEMEELESPVFLEDFPQDARTNPLARANTNNANANVAYGNSNHSNNQMFSSVHCEREFPGEAETPAAGRGALSHSHRALGPHSKPCAGKLNGQLVQPGMPINQAPPAPCQQPSTDPPERGQRRTSLTGSLQDEAPQRRSSEGSTPRRPAPATALLIQEALVRGGLDTLAADAGFVTATSQALADACQMEPEEVEVAATELLKARESVQGMASVPGSLSRRSSLGSLDQVQGSQETLIPPRP DIP protein P07293 original sequence version: 136 mint 047B10D1946B0796 ccg1_rabit Voltage-dependent calcium channel gamma-1 subunit CACNG1 CACNLG Dihydropyridine-sensitive L-type, skeletal muscle calcium channel subunit gamma protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MSPTEAPKVRVTLFCILVGIVLAMTAVVSDHWAVLSPHMENHNTTCEAAHFGLWRICTKRIALGEDRSCGPITLPGEKNCSYFRHFNPGESSEIFEFTTQKEYSISAAAISVFSLGFLIMGTICALMAFRKKRDYLLRPASMFYVFAGLCLFVSLEVMRQSVKRMIDSEDTVWIEYYYSWSFACACAAFVLLFLGGISLLLFSLPRMPQNPWESCMDAEPEH DIP protein P19518 original sequence version: 99 903B6FD8DD78F698 cacb1_rabit Voltage-dependent L-type calcium channel subunit beta-1 Calcium channel voltage-dependent subunit beta 1 CACNB1 CACNLB1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MVQKTSMSRGPYPPSQEIPMEVFDPSPQGKYSKRKGRFKRSDGSTSSDTTSNSFVRQGSAESYTSRPSDSDVSLEEDREALRKEAERQALAQLEKAKTKPVAFAVRTNVGYNPSPGDEVPVEGVAITFEPKDFLHIKEKYNNDWWIGRLVKEGCEVGFIPSPVKLDSLRLLQEQKLRQSRLSSSKSGDNSSSSLGDVVTGTRRPTPPASGNEMTNLAFELEPLDLEEDEAELGEQSGSAKTSVSSVTTPPPHGTRIPFFKKTEHVPPYDVVPSMRPIILVGPSLKGYEVTDMMQKALFDFLKHLFDGRISITRVTADISLAKRSVLNNPSKHIIIERSNTRSSLAEVQSEIERIFELARTLQLVALDADTINHPAQLSKTSLAPIIVYIKITSPKVLQRLIKSRGKSQSKHLNVQIAASEKLAQCPPEMFDIILDENQLEDACEHLAEYLEAYWKATHPPSSTPPNPLLNRTMATAALAASPAPVSNLQVQVLTSLRRNLSFWGGLETSQRGGGAVPQQQEHAM DIP protein P19517 original sequence version: 117 629420640A7457CC p13806-pro_0000005008 Voltage-dependent calcium channel subunit delta-1 CACNA2D1 CACNL2A CCHL2A Voltage-gated calcium channel subunit alpha-2/delta-1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit ADMEDDDFTASMSKQSCITEQTQYFFDNDSKSFSGVLDCGNCSRIFHVEKLMNTNLIFIMVESKGTCPCDTRLLIQAEQTSDGPDPCDMVKQPRYRKGPDVCFDNNVLEDYTDCGGVSGLNPSLWSIIGIQFVLLWLVSGSRHCLL 961 1106 BDAF12DD6A8B793C cacna1s-cacna2d1 1 4 unspecified role unspecified role neutral component neutral component purified purified rabbit-skeletal_muscle Oryctolagus cuniculus skeletal_muscle Skeletal muscle 5 unspecified role unspecified role neutral component neutral component purified purified rabbit-skeletal_muscle Oryctolagus cuniculus skeletal_muscle Skeletal muscle 6 unspecified role unspecified role neutral component neutral component purified purified rabbit-skeletal_muscle Oryctolagus cuniculus skeletal_muscle Skeletal muscle 7 unspecified role unspecified role neutral component neutral component purified purified cys102 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain Cys 102 in delta1 subunit forms a disulphide bridge with Cys 406 in alpha 2 subunit to form the alpha2/delta1 subunit. rabbit-skeletal_muscle Oryctolagus cuniculus skeletal_muscle Skeletal muscle 3 unspecified role unspecified role neutral component neutral component purified purified cys406 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain Cys 406 in alpha2 subunit forms a disulphide bridge with Cys 102 in delta1 subunit to form the alpha2/delta1 subunit. rabbit-skeletal_muscle Oryctolagus cuniculus skeletal_muscle Skeletal muscle 15 13 physical association physical association Fig. 1A, B, Fig. 3 and Fig. 6A cacna1s-cacna2d1-1 2 4 unspecified role unspecified role neutral component neutral component purified purified 3 unspecified role unspecified role neutral component neutral component purified purified cys406 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain Cys 406 in alpha2 subunit forms a disulphide bridge with Cys 102 in delta1 subunit to form the alpha2/delta1 subunit. 7 unspecified role unspecified role neutral component neutral component purified purified cys102 disulfide crosslinked residues disulfide crosslinked residues certain certain sequence position certain certain certain sequence position certain Cys 102 in delta1 subunit forms a disulphide bridge with Cys 406 in alpha 2 subunit to form the alpha2/delta1 subunit. 5 unspecified role unspecified role neutral component neutral component purified purified 6 unspecified role unspecified role neutral component neutral component purified purified physical association physical association Fig. 1A, B