mbinfo MBInfo http://www.mechanobio.info/ Identification of p130Cas as a mediator of focal adhesion kinase-promoted cell migration. Identification of p130Cas as a mediator of focal adhesion kinase-promoted cell migration. J. Cell Biol. (0021-9525) 1998 imex curation Cary L.A., Han D.C., Polte T.R., Hanks S.K., Guan J.L. Interaction detection method: GST pull down. The source of FAK and Src traced from references. Contact email not provided in the publication. 09-Sep-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay autoradiography autoradiography Cary L.A., Han D.C., Polte T.R., Hanks S.K., Guan J.L. Interaction detection method: GST pull down. The source of FAK and Src traced from references. Contact email not provided in the publication. Accepted 2013-AUG-15 by ORCHARD 09-Sep-2013: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 1998 Only protein-protein interactions imex curation Identification of p130Cas as a mediator of focal adhesion kinase-promoted cell migration. Identification of p130Cas as a mediator of focal adhesion kinase-promoted cell migration. J. Cell Biol. (0021-9525) 1998 imex curation Cary L.A., Han D.C., Polte T.R., Hanks S.K., Guan J.L. Interaction detection method: GST pull down. The source of FAK and Src traced from references. Contact email not provided in the publication. 09-Sep-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Cary L.A., Han D.C., Polte T.R., Hanks S.K., Guan J.L. Interaction detection method: GST pull down. The source of FAK and Src traced from references. Contact email not provided in the publication. Accepted 2013-AUG-15 by ORCHARD 09-Sep-2013: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 1998 Only protein-protein interactions imex curation Identification of p130Cas as a mediator of focal adhesion kinase-promoted cell migration. Identification of p130Cas as a mediator of focal adhesion kinase-promoted cell migration. J. Cell Biol. (0021-9525) 1998 imex curation Cary L.A., Han D.C., Polte T.R., Hanks S.K., Guan J.L. Interaction detection method: GST pull down. The source of FAK and Src traced from references. Contact email not provided in the publication. 09-Sep-2013: Contacted by IntAct-Help. Only protein-protein interactions crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Cary L.A., Han D.C., Polte T.R., Hanks S.K., Guan J.L. Interaction detection method: GST pull down. The source of FAK and Src traced from references. Contact email not provided in the publication. Accepted 2013-AUG-15 by ORCHARD 09-Sep-2013: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 1998 Only protein-protein interactions imex curation Identification of p130Cas as a mediator of focal adhesion kinase-promoted cell migration. Identification of p130Cas as a mediator of focal adhesion kinase-promoted cell migration. J. Cell Biol. (0021-9525) 1998 imex curation Cary L.A., Han D.C., Polte T.R., Hanks S.K., Guan J.L. Interaction detection method: GST pull down. The source of FAK and Src traced from references. Contact email not provided in the publication. 09-Sep-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Cary L.A., Han D.C., Polte T.R., Hanks S.K., Guan J.L. Interaction detection method: GST pull down. The source of FAK and Src traced from references. Contact email not provided in the publication. Accepted 2013-AUG-15 by ORCHARD 09-Sep-2013: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 1998 Only protein-protein interactions imex curation Identification of p130Cas as a mediator of focal adhesion kinase-promoted cell migration. Identification of p130Cas as a mediator of focal adhesion kinase-promoted cell migration. J. Cell Biol. (0021-9525) 1998 imex curation Cary L.A., Han D.C., Polte T.R., Hanks S.K., Guan J.L. Interaction detection method: GST pull down. The source of FAK and Src traced from references. Contact email not provided in the publication. 09-Sep-2013: Contacted by IntAct-Help. Only protein-protein interactions crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Cary L.A., Han D.C., Polte T.R., Hanks S.K., Guan J.L. Interaction detection method: GST pull down. The source of FAK and Src traced from references. Contact email not provided in the publication. Accepted 2013-AUG-15 by ORCHARD 09-Sep-2013: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 1998 Only protein-protein interactions imex curation grb2_human Growth factor receptor-bound protein 2 GRB2 ASH Adapter protein GRB2 SH2/SH3 adapter GRB2 Protein Ash protein protein human Homo sapiens Human MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV 83A4B0BA1B248DC4 fak1_chick Focal adhesion kinase 1 PTK2 FAK FAK1 pp125FAK Protein-tyrosine kinase 2 Focal adhesion kinase-related nonkinase p125FAK protein protein chick Gallus gallus (Chicken) Chicken MAAAYLDPNLNHTPSSSAKTHLGTGMERSPGAMERVLKVFHYFENSSEPTTWASIIRHGDATDVRGIIQKIVDCHKVKNVACYGLRLSHLQSEEVHWLHLDMGVSNVREKFELAHPPEEWKYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKNDYMLEIADQVDQEIALKLGCLEIRRSYGEMRGNALEKKSNYEVLEKDVGLRRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTDKGANPTHLADFNQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGATQSFIIRPQKEGERALPSIPKLANNEKQGVRSHTVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRERIELGRCIGEGQFGDVHQGIYMSPENPAMAVAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKFSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSATDCVKLGDFGLSRYMEDSTYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKLQQEERMRMESRRQVTVSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQPNHYQVSGYSGSHGIPAMAGSIYPGQASLLDQTDSWNHRPQEVSAWQPNMEDSGTLDVRGMGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLVMKPDVRLSRGSIEREDGGLQGPAGNQHIYQPVGKPDHAAPPKKPPRPGAPHLGSLASLNSPVDSYNEGVKIKPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDESLPVLPASTHREIEMAQKLLNSDLAELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMISQSRPH 8051154219B953B9 p130cas_crigr_protein p130Cas protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster bcar1_mouse Breast cancer anti-estrogen resistance protein 1 Bcar1 Cas Crkas p130cas CRK-associated substrate protein protein mouse Mus musculus Mouse MTVPNVLAKALYDNVAESPDELSFRKGDIMTVLERDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPVGPGPGPPATPPQPQPSLPQGVHAPVPPASQYSPMLPTAYQPQSDNVYLVPTPSKTQQGLYQAPGPNPQFQSPPAKQTSTFSKQTPHHSFPSPATDLYQVPPGPGSPAQDIYQVPPSAGIGHDIYQVPPSLDTRGWEGTKPPAKVVVPTRVGQGYVYEAAQTEQDEYDTPRHLLAPGPQDIYDVPPVRGLLPNQYGQEVYDTPPMAVKGPNGRDPLLDVYDVPPSVEKGLLSSSHHSVYDVPPSVSKDVPDGPLLREETYDVPPAFAKPKPFDPTRHPLILAAPPPDSPAAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGSVVDDGVYAVPPPAEREAPTDGKRLSASSTGSTRSSQSASSLEVVVPGREPLELEVAVESLARLQQGVSTTVAHLLDLVGSASGPGGWRGTSEPQEPPAQDLKAAVAAVHGAVHELLEFARGAVSNATHTSDRTLHAKLSRQLQKMEDVYQTLVVHGQVLDSGRGSPGFTPEDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKAPGPGPEGSSSLHPNPTDKASSIQSRPLPSPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGKEEFEKTQKELLERGNIMRQGKGQLELQQLKQFERLEQEVSRPIDHDLANWTPAQPLVPGRTGGLGPSDRQLLLFYLEQCEANLTTLTDAVDAFFTAVATNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSPSAAQDMVDRVKELGHSTQQFRRVLGQLAAA 81E56BC7AD87B095 g3gxx7_crigr Tyrosine-protein kinase CgPICR_001167 I79_002622 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MGSNKSKPKDASQRRRSLEPAENVHGAGGAFPASQTPSKPASADGHRGPSAAFVATTAAESKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLSTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKGETGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL 6EF52B803F097843 p85a_bovin Phosphatidylinositol 3-kinase regulatory subunit alpha Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha PIK3R1 protein protein bovin Bos taurus (Bovine) Bovine MSAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSDGQEAKPEEIGWLNGYNETTGERGDFPGTYVEYIGRKKISPPTPKPRPPRPLPVAPGPSKTEADSEQQASTLPDLAEQFAPPDVAPPLLIKLVEAIEKKGLECSTLYRTQSSSNPAELRQLLDCDTASLDLEMFDVHVLADAFKRYLLDLPNPVIPVAVSSELISLAPEVQSSEEYIQLLKKLIRSPSIPHQYWLTLQYLLKHFFKLSQTSSKNLLNARVLSELFSPLLFRFPAASSENTEHLIKIIEILISTEWNERQPAPALPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFNSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEDYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNETEIQRIMHNYEKLKSRISEIVDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR DIP protein P23727 original sequence version: 108 EBDF6E754BBF7321 src_chick Proto-oncogene tyrosine-protein kinase Src SRC pp60c-src Proto-oncogene c-Src protein protein chick Gallus gallus (Chicken) Chicken MGSSKSKPKDPSQRRRSLEPPDSTHHGGFPASQTPNKTAAPDTHRTPSRSFGTVATEPKLFGGFNTSDTVTSPQRAGALAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLTTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNPENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFSSLQQLVAYYSKHADGLCHRLTNVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKGEMGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKDPEERPTFEYLQAFLEDYFTSTEPQYQPGENL DIP protein P00523 original sequence version: 105 ABDB036F7D63C30A src-ptk2-1 2 7 unspecified role unspecified role prey prey n-terminal region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q00944:p.Tyr397Phe mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 18:51:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr397phe' Tue Jun 30 18:29:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr397Phe' c-terminal t-antigen tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range FAK C-terminus was tagged with T-antigen epitope. crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 12 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9bact Bacteria physical association physical association Fig 2A. grb2-ptk2-2 4 7 unspecified role unspecified role prey prey n-terminal region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range FAK1 was tagged with three repeats of HA epitope at the amino terminus. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9bact Bacteria physical association physical association Fig 2B. Grb2-SH2 interacted with FAK tagged with HA epitope at the amino terminus, but did not interact with FAK tagged with HA tag at the amino terminus and T-antigen tag at the carboxyl terminus. ptk2-1 1 7 unspecified role unspecified role putative self putative self n-terminal region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal t-antigen tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range The C-terminal region of FAK was tagged with T-antigen epitope. Q00944:p.[Pro712Ala;Pro715Ala] mutation increasing rate mutation increasing interaction rate mutation increasing rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain P A Wed May 02 17:59:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro712ala,pro715ala' Tue Jun 30 18:37:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro712Ala;Pro715Ala]' crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. putative self interaction putative self interaction true Fig 4C. g3gxx7-ptk2 5 7 unspecified role unspecified role prey prey n-terminal region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal region tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range The C-terminal region of FAK was tagged with T-antigen epitope. Q00944:p.Tyr397Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 14:54:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr397phe' Tue Jun 30 17:45:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr397Phe' 10 unspecified role unspecified role bait bait physical association physical association Fig 5B. p130cas-ptk2-1 5 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey Q00944:p.[Pro712Ala;Pro715Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain P A Wed May 02 16:41:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro712ala,pro715ala' Tue Jun 30 16:48:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro712Ala;Pro715Ala]' n-terminal region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal region tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range The C-terminal region of FAK was tagged with T-antigen epitope. physical association physical association Fig 6B. src-p130cas-2 4 8 unspecified role unspecified role prey prey crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 12 unspecified role unspecified role bait bait sh2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9bact Bacteria physical association physical association Fig 7D. Src interacted with p130Cas when GST-Src was incubated with p130Cas immunoprecipitated from cells transfected with FAK-WT and FAK-KD. Src interaction with p130Cas was diminished when GST-Src was incubated with p130Cas immunoprecipitated from cells transfected with FAK-Y397F and FAK-P712/715A. pik3r1-p130cas 4 8 unspecified role unspecified role prey prey crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-sh2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9bact Bacteria physical association physical association Fig 7F. p85 interacted with p130Cas when GST-p85 was incubated with p130Cas immunoprecipitated from cells transfected with FAK-WT and FAK-KD. p85 interaction with p130Cas was diminished when GST-p85 was incubated with p130Cas immunoprecipitated from cells transfected with FAK-Y397F and FAK-P712/715A. ptk2-bcar1-2 3 7 unspecified role unspecified role bait bait n-terminal region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal region tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range The c-terminal region of FAK was tagged with T-antigen epitope. 9 unspecified role unspecified role prey prey n-terminal region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range The N-terminus of p130Cas was tagged with three repeats of the HA tag. physical association physical association Fig 10 C.