IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Properties and structure of spermidine acetyltransferase in Escherichia coli. Properties and structure of spermidine acetyltransferase in Escherichia coli. J. Biol. Chem. (0021-9258) 1994 imex curation Fukuchi J., Kashiwagi K., Takio K., Igarashi K. 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Fukuchi J., Kashiwagi K., Takio K., Igarashi K. Accepted 2013-NOV-21 by ORCHARD J. Biol. Chem. (0021-9258) 1994 Only protein-protein interactions imex curation Properties and structure of spermidine acetyltransferase in Escherichia coli. Properties and structure of spermidine acetyltransferase in Escherichia coli. J. Biol. Chem. (0021-9258) 1994 imex curation Fukuchi J., Kashiwagi K., Takio K., Igarashi K. 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro acetylase assay acetylase assay predetermined predetermined participant predetermined Fukuchi J., Kashiwagi K., Takio K., Igarashi K. Accepted 2013-NOV-21 by ORCHARD J. Biol. Chem. (0021-9258) 1994 Only protein-protein interactions imex curation spermidine spermidine 1,5,10-triazadecane 4-azaoctamethylenediamine 4-azaoctane-1,8-diamine N-(3-Aminopropyl)-1,4-butane-diamine Spermidin SPERMIDINE Spermidine N-(3-aminopropyl)butane-1,4-diamine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ATHGHQPFGPMSJY-UHFFFAOYSA-N ATHGHQPFGPMSJY-UHFFFAOYSA-N acetyl-coa small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) atda_ecoli Spermidine N(1)-acetyltransferase speG Spermidine/spermine N(1)-acetyltransferase JW1576 b1584 protein protein ecoli Escherichia coli (strain K12) MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVELSDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAIRMCIFQHQYLAEHKTPGQTLLKPTAQ 27297083B93B3752 speg-1 1 5 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3 acetyl_coa-speg-1 2 5 enzyme enzyme neutral component neutral component ecoli Escherichia coli (strain K12) 4 ancillary ancillary neutral component neutral component acetyl-1-[14]c 14c radiolabel 14c radiolabel C14 14C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 3 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) acetylation acetylation reaction acetylation Fig. 2 BESPD and BESPM competitively inhibit atda activity. Spermidine as substrate: Km [acetyle-CoA] = 2.0 um Ki [CoA] = 3.6 um Ki [ADP] = 107 um Acetyl-CoA as substrate: Km [spermidine] = 1.29 um Km [spermine] = 220 um Inhibitors: Ki [BESPD] = 1.57 um Ki [BESPM] = 645 um