MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Quality control of a molybdoenzyme by the Lon protease. Quality control of a molybdoenzyme by the Lon protease. FEBS letters 2013 imex curation David Redelberger, Olivier Genest, Dallel Arabet, Vincent Mejean, Marianne Ilbert, chantal iobbi-nivol, Redelberger D., Genest O., Arabet D., Méjean V., Ilbert M., Iobbi-Nivol C., Redelberger D., Genest O., Arabet D., Méjean V., Ilbert M., Iobbi-Nivol C. iobbi@imm.cnrs.fr 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined David Redelberger; Olivier Genest; Dallel Arabet; Vincent Mejean; Marianne Ilbert; chantal iobbi-nivol iobbi@imm.cnrs.fr Accepted 2013-OCT-30 by LUANA 22-Nov-2013: Contacted by IntAct-Help. 2013 Only protein-protein interactions imex curation Quality control of a molybdoenzyme by the Lon protease. Quality control of a molybdoenzyme by the Lon protease. FEBS letters 2013 imex curation David Redelberger, Olivier Genest, Dallel Arabet, Vincent Mejean, Marianne Ilbert, chantal iobbi-nivol, Redelberger D., Genest O., Arabet D., Méjean V., Ilbert M., Iobbi-Nivol C., Redelberger D., Genest O., Arabet D., Méjean V., Ilbert M., Iobbi-Nivol C. iobbi@imm.cnrs.fr 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined David Redelberger; Olivier Genest; Dallel Arabet; Vincent Mejean; Marianne Ilbert; chantal iobbi-nivol iobbi@imm.cnrs.fr Accepted 2013-OCT-30 by LUANA 22-Nov-2013: Contacted by IntAct-Help. 2013 Only protein-protein interactions imex curation lon_ecoli Lon protease lon capR deg lopA muc b0439 ATP-dependent protease La JW0429 protein protein ecoli Escherichia coli (strain K12) MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAMDHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISALSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSIDDPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVKKQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQKLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRILEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIRGHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNVAFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLPKQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLKHIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKLTYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGIAMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFENKRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK DIP protein P0A9M0 original sequence version: 47 DIP protein P0A9M0 original sequence version: 95 4042499C97694EF8 tora_ecoli Trimethylamine-N-oxide reductase 1 torA b0997 JW0982 protein protein ecoli Escherichia coli (strain K12) MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAATDAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRYPMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGWQSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLVLQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTHEYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEKDGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLAAMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGLRKLETVIAIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS DIP protein P33225 original sequence version: null 59DDACB00B1843E7 tora-lon 1 apoTorA 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Lon 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction f2 The first set of results suggests that Lon recognizes only the core of apoTorA. To confirm this point, a surface plasmon resonance (SPR) experiment was carried out to follow, in real time, the interaction between Lon and the apo or holo form of TorA. The data show an interaction between Lon and apoTorA (Fig. 2),. Moreover, this data set shows the formation of a very stable complex between Lon and apoTorA. The determination of the equilibrium constant level (Req) at different concentrations allows us to estimate after three independent experiments a Kd of 1.0 ± 0.2 μM. In contrast, no interaction was detected with holoTorA (Fig.2 inset) confirming that the protease binds the core of TorA only in the absence of the cofactor. lon-tora 2 apoTorA 4 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Lon 3 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cleavage cleavage reaction cleavage f4a f4c