IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Phosphorylation of the Actin Binding Protein Drebrin at S647 Is Regulated by Neuronal Activity and PTEN. Phosphorylation of the Actin Binding Protein Drebrin at S647 Is Regulated by Neuronal Activity and PTEN. PLoS ONE 2013 imex curation Kreis P., Hendricusdottir R., Kay L., Papageorgiou I.E., van Diepen M., Mack T., Ryves J., Harwood A., Leslie N.R., Kann O., Parsons M., Eickholt B.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease Parkinsons - Interactions investigated in the context of Parkinsons disease "hippocampal" neurons:- could be either Rat or Mouse. Where it says "E18" - this is Rat. (mouse not needed for interactions entered here). Britta.Eickholt@charite.de 14-Oct-2013: Contacted by IntAct-Help. Only protein-protein interactions rat-brain Rattus norvegicus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip sequence tag sequence tag identification sequence tag Kreis P., Hendricusdottir R., Kay L., Papageorgiou I.E., van Diepen M., Mack T., Ryves J., Harwood A., Leslie N.R., Kann O., Parsons M., Eickholt B.J. Alzheimers - Interactions investigated in the context of Alzheimers disease "hippocampal" neurons:- could be either Rat or Mouse. Where it says "E18" - this is Rat. (mouse not needed for interactions entered here). Britta.Eickholt@charite.de Accepted 2013-OCT-02 by ORCHARD 14-Oct-2013: Contacted by IntAct-Help. PLoS ONE 2013 Only protein-protein interactions imex curation Phosphorylation of the Actin Binding Protein Drebrin at S647 Is Regulated by Neuronal Activity and PTEN. Phosphorylation of the Actin Binding Protein Drebrin at S647 Is Regulated by Neuronal Activity and PTEN. PLoS ONE 2013 imex curation Kreis P., Hendricusdottir R., Kay L., Papageorgiou I.E., van Diepen M., Mack T., Ryves J., Harwood A., Leslie N.R., Kann O., Parsons M., Eickholt B.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease Parkinsons - Interactions investigated in the context of Parkinsons disease "hippocampal" neurons:- could be either Rat or Mouse. Where it says "E18" - this is Rat. (mouse not needed for interactions entered here). Britta.Eickholt@charite.de 14-Oct-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kreis P., Hendricusdottir R., Kay L., Papageorgiou I.E., van Diepen M., Mack T., Ryves J., Harwood A., Leslie N.R., Kann O., Parsons M., Eickholt B.J. Alzheimers - Interactions investigated in the context of Alzheimers disease "hippocampal" neurons:- could be either Rat or Mouse. Where it says "E18" - this is Rat. (mouse not needed for interactions entered here). Britta.Eickholt@charite.de Accepted 2013-OCT-02 by ORCHARD 14-Oct-2013: Contacted by IntAct-Help. PLoS ONE 2013 Only protein-protein interactions imex curation Phosphorylation of the Actin Binding Protein Drebrin at S647 Is Regulated by Neuronal Activity and PTEN. Phosphorylation of the Actin Binding Protein Drebrin at S647 Is Regulated by Neuronal Activity and PTEN. PLoS ONE 2013 imex curation Kreis P., Hendricusdottir R., Kay L., Papageorgiou I.E., van Diepen M., Mack T., Ryves J., Harwood A., Leslie N.R., Kann O., Parsons M., Eickholt B.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease Parkinsons - Interactions investigated in the context of Parkinsons disease "hippocampal" neurons:- could be either Rat or Mouse. Where it says "E18" - this is Rat. (mouse not needed for interactions entered here). Britta.Eickholt@charite.de 14-Oct-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kreis P., Hendricusdottir R., Kay L., Papageorgiou I.E., van Diepen M., Mack T., Ryves J., Harwood A., Leslie N.R., Kann O., Parsons M., Eickholt B.J. Alzheimers - Interactions investigated in the context of Alzheimers disease "hippocampal" neurons:- could be either Rat or Mouse. Where it says "E18" - this is Rat. (mouse not needed for interactions entered here). Britta.Eickholt@charite.de Accepted 2013-OCT-02 by ORCHARD 14-Oct-2013: Contacted by IntAct-Help. PLoS ONE 2013 Only protein-protein interactions imex curation Phosphorylation of the Actin Binding Protein Drebrin at S647 Is Regulated by Neuronal Activity and PTEN. Phosphorylation of the Actin Binding Protein Drebrin at S647 Is Regulated by Neuronal Activity and PTEN. PLoS ONE 2013 imex curation Kreis P., Hendricusdottir R., Kay L., Papageorgiou I.E., van Diepen M., Mack T., Ryves J., Harwood A., Leslie N.R., Kann O., Parsons M., Eickholt B.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease Parkinsons - Interactions investigated in the context of Parkinsons disease "hippocampal" neurons:- could be either Rat or Mouse. Where it says "E18" - this is Rat. (mouse not needed for interactions entered here). Britta.Eickholt@charite.de 14-Oct-2013: Contacted by IntAct-Help. Only protein-protein interactions rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Kreis P., Hendricusdottir R., Kay L., Papageorgiou I.E., van Diepen M., Mack T., Ryves J., Harwood A., Leslie N.R., Kann O., Parsons M., Eickholt B.J. Alzheimers - Interactions investigated in the context of Alzheimers disease "hippocampal" neurons:- could be either Rat or Mouse. Where it says "E18" - this is Rat. (mouse not needed for interactions entered here). FLIM:- multiphoton fluorescence-lifetime imaging microscopy Britta.Eickholt@charite.de Accepted 2013-OCT-02 by ORCHARD 14-Oct-2013: Contacted by IntAct-Help. PLoS ONE 2013 Only protein-protein interactions imex curation Phosphorylation of the Actin Binding Protein Drebrin at S647 Is Regulated by Neuronal Activity and PTEN. Phosphorylation of the Actin Binding Protein Drebrin at S647 Is Regulated by Neuronal Activity and PTEN. PLoS ONE 2013 imex curation Kreis P., Hendricusdottir R., Kay L., Papageorgiou I.E., van Diepen M., Mack T., Ryves J., Harwood A., Leslie N.R., Kann O., Parsons M., Eickholt B.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease Parkinsons - Interactions investigated in the context of Parkinsons disease "hippocampal" neurons:- could be either Rat or Mouse. Where it says "E18" - this is Rat. (mouse not needed for interactions entered here). Britta.Eickholt@charite.de 14-Oct-2013: Contacted by IntAct-Help. Only protein-protein interactions rat-brain Rattus norvegicus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kreis P., Hendricusdottir R., Kay L., Papageorgiou I.E., van Diepen M., Mack T., Ryves J., Harwood A., Leslie N.R., Kann O., Parsons M., Eickholt B.J. Alzheimers - Interactions investigated in the context of Alzheimers disease "hippocampal" neurons:- could be either Rat or Mouse. Where it says "E18" - this is Rat. (mouse not needed for interactions entered here). Britta.Eickholt@charite.de Accepted 2013-OCT-02 by ORCHARD 14-Oct-2013: Contacted by IntAct-Help. PLoS ONE 2013 Only protein-protein interactions imex curation Phosphorylation of the Actin Binding Protein Drebrin at S647 Is Regulated by Neuronal Activity and PTEN. Phosphorylation of the Actin Binding Protein Drebrin at S647 Is Regulated by Neuronal Activity and PTEN. PLoS ONE 2013 imex curation Kreis P., Hendricusdottir R., Kay L., Papageorgiou I.E., van Diepen M., Mack T., Ryves J., Harwood A., Leslie N.R., Kann O., Parsons M., Eickholt B.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease Parkinsons - Interactions investigated in the context of Parkinsons disease "hippocampal" neurons:- could be either Rat or Mouse. Where it says "E18" - this is Rat. (mouse not needed for interactions entered here). Britta.Eickholt@charite.de 14-Oct-2013: Contacted by IntAct-Help. Only protein-protein interactions rat-liver rattus norvegicus liver Rat liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip sequence tag sequence tag identification sequence tag Kreis P., Hendricusdottir R., Kay L., Papageorgiou I.E., van Diepen M., Mack T., Ryves J., Harwood A., Leslie N.R., Kann O., Parsons M., Eickholt B.J. Alzheimers - Interactions investigated in the context of Alzheimers disease "hippocampal" neurons:- could be either Rat or Mouse. Where it says "E18" - this is Rat. (mouse not needed for interactions entered here). Britta.Eickholt@charite.de Accepted 2013-OCT-02 by ORCHARD 14-Oct-2013: Contacted by IntAct-Help. PLoS ONE 2013 Only protein-protein interactions imex curation Phosphorylation of the Actin Binding Protein Drebrin at S647 Is Regulated by Neuronal Activity and PTEN. Phosphorylation of the Actin Binding Protein Drebrin at S647 Is Regulated by Neuronal Activity and PTEN. PLoS ONE 2013 imex curation Kreis P., Hendricusdottir R., Kay L., Papageorgiou I.E., van Diepen M., Mack T., Ryves J., Harwood A., Leslie N.R., Kann O., Parsons M., Eickholt B.J. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease Parkinsons - Interactions investigated in the context of Parkinsons disease "hippocampal" neurons:- could be either Rat or Mouse. Where it says "E18" - this is Rat. (mouse not needed for interactions entered here). Britta.Eickholt@charite.de 14-Oct-2013: Contacted by IntAct-Help. Only protein-protein interactions rat-primary_hippocampal_neuron Rat primary hippocampal neuron primary hippocampal neurons Rat primary hippocampal neurons fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Kreis P., Hendricusdottir R., Kay L., Papageorgiou I.E., van Diepen M., Mack T., Ryves J., Harwood A., Leslie N.R., Kann O., Parsons M., Eickholt B.J. Alzheimers - Interactions investigated in the context of Alzheimers disease "hippocampal" neurons:- could be either Rat or Mouse. Where it says "E18" - this is Rat. (mouse not needed for interactions entered here). FLIM:- multiphoton fluorescence-lifetime imaging microscopy Britta.Eickholt@charite.de Accepted 2013-OCT-02 by ORCHARD 14-Oct-2013: Contacted by IntAct-Help. PLoS ONE 2013 Only protein-protein interactions imex curation pten_human Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Phosphatase and tensin homolog Mutated in multiple advanced cancers 1 PTEN MMAC1 TEP1 protein protein human Homo sapiens Human MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV 75F97C3DD6802BA9 o54857_rat Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Phosphatase and tensin homolog Pten PTEN/MMAC1 rCG_47874 protein protein rat Rattus norvegicus (Rat) Rat MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKLMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHSQITKV mint 243BFE35FE209FE5 dreb_human Drebrin DBN1 Developmentally-regulated brain protein D0S117E protein protein human Homo sapiens Human MAGVSFSGHRLELLAAYEEVIREESAADWALYTYEDGSDDLKLAASGEGGLQELSGHFENQKVMYGFCSVKDSQAALPKYVLINWVGEDVPDARKCACASHVAKVAEFFQGVDVIVNASSVEDIDAGAIGQRLSNGLARLSSPVLHRLRLREDENAEPVGTTYQKTDAAVEMKRINREQFWEQAKKEEELRKEEERKKALDERLRFEQERMEQERQEQEERERRYREREQQIEEHRRKQQTLEAEEAKRRLKEQSIFGDHRDEEEETHMKKSESEVEEAAAIIAQRPDNPREFFKQQERVASASAGSCDVPSPFNHRPGSHLDSHRRMAPTPIPTRSPSDSSTASTPVAEQIERALDEVTSSQPPPLPPPPPPAQETQEPSPILDSEETRAAAPQAWAGPMEEPPQAQAPPRGPGSPAEDLMFMESAEQAVLAAPVEPATADATEIHDAADTIETDTATADTTVANNVPPAATSLIDLWPGNGEGASTLQGEPRAPTPPSGTEVTLAEVPLLDEVAPEPLLPAGEGCATLLNFDELPEPPATFCDPEEVEGESLAAPQTPTLPSALEELEQEQEPEPHLLTNGETTQKEGTQASEGYFSQSQEEEFAQSEELCAKAPPPVFYNKPPEIDITCWDADPVPEEEEGFEGGD A7DF1AE3776C0BEA dreb_rat Drebrin Dbn1 Developmentally-regulated brain protein protein protein rat Rattus norvegicus (Rat) Rat MAGVSFSGHRLELLAAYEEVIREESAADWALYTYEDGSDDLKLAASGEGGLQELSGHFENQKVMYGFCSVKDSQAALPKYVLINWVGEDVPDARKCACASHVAKVAEFFQGVDVIVNASSVEDIDAGAIGQRLSNGLARLSSPVLHRLRLREDENAEPVGTTYQKTDAAVEMKRINREQFWEQAKKEEELRKEEERKKALDARLRFEQERMEQERQEQEERERRYREREQQIEEHRRKQQSLEAEEAKRRLKDQSIFGDQRDEEEESQMKKSESEVEEAAAIIAQRPDNPREFFRQQERVASASGGSCDAPSPFNHRPGRPYCPFIKASDSGPSSSSSSSSSPPRTPFPYITCHRTPNLSSSLPCSHLDSHRRMAPTPIPTRSPSDSSTASTPITEQIERALDEVTSSQPPPPPPPPPPAQEAQESAPRLDGEEVCKEAKVAAAPQVWAGCAEEPPRAQEPPLLQSSPTEDLMCTESPEQAVLAASPEPDASVTSVADAHAADTIETTTATTATTIADNVTPAAASLIDLWPGNGEEASTPQAEPRVPTPPSGAEASLAEVPLLNEAAQEPLPPVGEGCANLLNFDELPEPPATFCDPEEEAEGEPLAASQVLTMPSALEEVDQVLEQELEPEPHLLTNGETTQKEGTQQASEGYFSQSQEEEFAQSEEPCAKAPPPVFYNKPPEIDITCWDADPVPEEEEGFEGGD B5279BF6EB7B80AA pten-dbn1 1 9 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 1A pten-dbn1-1 6 9 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 1A pten-dbn1-2 5 9 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 1B pten-dbn1-3 2 10 unspecified role unspecified role prey prey n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role bait bait n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1C dbn1-pten 2 10 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role prey prey n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1C dbn1-pten-1 3 8 unspecified role unspecified role prey prey n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c2_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain deleted_loop binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1E/F A PTEN fragment encompassing only the structurally defined C2 domain (amino-acid 182-354; PTEN C2) greatly increased the interaction with DBN. Deletions of the D-loop (aa 286-309) from full length PTEN (PTENΔD) and from the PTEN C2-domain (PTENC2ΔD) decreased the DBN interaction. I cannot add the aa 286-309 -deleted loop - region as a "required to bind" - as there is still binding when it is deleted! - so "binding region" would seem a reasonable compromise? - or delete this feature? dbn1-pten-4 4 8 unspecified role unspecified role fluorescence donor fluorescence donor delta_d_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction 2A/B Deletions of the D-loop (aa 286-309) from full length PTEN (PTENΔD) and from the PTEN C2-domain (PTENC2ΔD) decreased the DBN interaction. dbn1-pten-5 7 8 unspecified role unspecified role fluorescence donor fluorescence donor region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined delta_d_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction 2C, 7A. Exposure to KCl for 5 minutes resulted in a 45% decrease in FRET efficiencies. Membrane depolarization (caused by KCl treatment) induces a dissociation of the PTEN:DBN complex.