IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Actin-binding Protein Drebrin Regulates HIV-1-triggered Actin Polymerization and Viral Infection. Actin-binding Protein Drebrin Regulates HIV-1-triggered Actin Polymerization and Viral Infection. J. Biol. Chem. (0021-9258) 2013 imex curation Gordón-Alonso M., Rocha-Perugini V., Alvarez S., Ursa A., Izquierdo-Useros N., Martinez-Picado J., Muñoz-Fernández M.A., Sánchez-Madrid F. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease CEM-T4 T cell line:a naturally isolated subclone of the CEM (ccrf_cem) line with high levels of surface CD4 expression. These cells were incubated with or without free HIV-1 viral particles, which induce "capping". GFP-Drebrin has been entered as of Rattine origin- see Ref 41 ( Hayashi et al., 1999). fsanchez.hlpr@salud.madrid.org 14-Oct-2013: Contacted by IntAct-Help. Only protein-protein interactions human-ccrf_cem Homo sapiens T-Acute Lymphoblastic Leukemia cell line ccrf_cem T-Acute Lymphoblastic Leukemia cell line confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Gordón-Alonso M., Rocha-Perugini V., Alvarez S., Ursa A., Izquierdo-Useros N., Martinez-Picado J., Muñoz-Fernández M.A., Sánchez-Madrid F. Parkinsons - Interactions investigated in the context of Parkinsons disease CEM-T4 T cell line:a naturally isolated subclone of the CEM (ccrf_cem) line with high levels of surface CD4 expression. These cells were incubated with or without free HIV-1 viral particles, which induce "capping". GFP-Drebrin has been entered as of Rattine origin- see Ref 41 ( Hayashi et al., 1999). fsanchez.hlpr@salud.madrid.org Accepted 2013-OCT-03 by PPORRAS 14-Oct-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2013 Only protein-protein interactions imex curation Actin-binding Protein Drebrin Regulates HIV-1-triggered Actin Polymerization and Viral Infection. Actin-binding Protein Drebrin Regulates HIV-1-triggered Actin Polymerization and Viral Infection. J. Biol. Chem. (0021-9258) 2013 imex curation Gordón-Alonso M., Rocha-Perugini V., Alvarez S., Ursa A., Izquierdo-Useros N., Martinez-Picado J., Muñoz-Fernández M.A., Sánchez-Madrid F. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease CEM-T4 T cell line:a naturally isolated subclone of the CEM (ccrf_cem) line with high levels of surface CD4 expression. These cells were incubated with or without free HIV-1 viral particles, which induce "capping". GFP-Drebrin has been entered as of Rattine origin- see Ref 41 ( Hayashi et al., 1999). fsanchez.hlpr@salud.madrid.org 14-Oct-2013: Contacted by IntAct-Help. Only protein-protein interactions human-jurkat Homo sapiens jurkat Human T cell leukemia anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gordón-Alonso M., Rocha-Perugini V., Alvarez S., Ursa A., Izquierdo-Useros N., Martinez-Picado J., Muñoz-Fernández M.A., Sánchez-Madrid F. Parkinsons - Interactions investigated in the context of Parkinsons disease CEM-T4 T cell line:a naturally isolated subclone of the CEM (ccrf_cem) line with high levels of surface CD4 expression. These cells were incubated with or without free HIV-1 viral particles, which induce "capping". CEM-T4 T cell line:a naturally isolated subclone of the CEM (ccrf_cem) line with high levels of surface CD4 expression. These cells were incubated with or without free HIV-1 viral particles, which induce "capping". GFP-Drebrin has been entered as of Rattine origin- see Ref 41 ( Hayashi et al., 1999). fsanchez.hlpr@salud.madrid.org Accepted 2013-OCT-03 by PPORRAS 14-Oct-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2013 Only protein-protein interactions imex curation cxcr4_human C-X-C chemokine receptor type 4 Stromal cell-derived factor 1 receptor Fusin Leukocyte-derived seven transmembrane domain receptor LCR1 FB22 NPYRL HM89 CXCR4 Lipopolysaccharide-associated protein 3 protein protein human Homo sapiens Human MEGISIYTSDNYTEEMGSGDYDSMKEPCFREENANFNKIFLPTIYSIIFLTGIVGNGLVILVMGYQKKLRSMTDKYRLHLSVADLLFVITLPFWAVDAVANWYFGNFLCKAVHVIYTVNLYSSVLILAFISLDRYLAIVHATNSQRPRKLLAEKVVYVGVWIPALLLTIPDFIFANVSEADDRYICDRFYPNDLWVVVFQFQHIMVGLILPGIVILSCYCIIISKLSHSKGHQKRKALKTTVILILAFFACWLPYYIGISIDSFILLEIIKQGCEFENTVHKWISITEALAFFHCCLNPILYAFLGAKFKTSAQHALTSVSRGSSLKILSKGKRGGHSSVSTESESSSFHSS DIP protein P61073 original sequence version: 80 8C8476A186786B83 dreb_rat Drebrin Dbn1 Developmentally-regulated brain protein protein protein rat Rattus norvegicus (Rat) Rat MAGVSFSGHRLELLAAYEEVIREESAADWALYTYEDGSDDLKLAASGEGGLQELSGHFENQKVMYGFCSVKDSQAALPKYVLINWVGEDVPDARKCACASHVAKVAEFFQGVDVIVNASSVEDIDAGAIGQRLSNGLARLSSPVLHRLRLREDENAEPVGTTYQKTDAAVEMKRINREQFWEQAKKEEELRKEEERKKALDARLRFEQERMEQERQEQEERERRYREREQQIEEHRRKQQSLEAEEAKRRLKDQSIFGDQRDEEEESQMKKSESEVEEAAAIIAQRPDNPREFFRQQERVASASGGSCDAPSPFNHRPGRPYCPFIKASDSGPSSSSSSSSSPPRTPFPYITCHRTPNLSSSLPCSHLDSHRRMAPTPIPTRSPSDSSTASTPITEQIERALDEVTSSQPPPPPPPPPPAQEAQESAPRLDGEEVCKEAKVAAAPQVWAGCAEEPPRAQEPPLLQSSPTEDLMCTESPEQAVLAASPEPDASVTSVADAHAADTIETTTATTATTIADNVTPAAASLIDLWPGNGEEASTPQAEPRVPTPPSGAEASLAEVPLLNEAAQEPLPPVGEGCANLLNFDELPEPPATFCDPEEEAEGEPLAASQVLTMPSALEEVDQVLEQELEPEPHLLTNGETTQKEGTQQASEGYFSQSQEEEFAQSEEPCAKAPPPVFYNKPPEIDITCWDADPVPEEEEGFEGGD B5279BF6EB7B80AA dreb_human Drebrin DBN1 Developmentally-regulated brain protein D0S117E protein protein human Homo sapiens Human MAGVSFSGHRLELLAAYEEVIREESAADWALYTYEDGSDDLKLAASGEGGLQELSGHFENQKVMYGFCSVKDSQAALPKYVLINWVGEDVPDARKCACASHVAKVAEFFQGVDVIVNASSVEDIDAGAIGQRLSNGLARLSSPVLHRLRLREDENAEPVGTTYQKTDAAVEMKRINREQFWEQAKKEEELRKEEERKKALDERLRFEQERMEQERQEQEERERRYREREQQIEEHRRKQQTLEAEEAKRRLKEQSIFGDHRDEEEETHMKKSESEVEEAAAIIAQRPDNPREFFKQQERVASASAGSCDVPSPFNHRPGSHLDSHRRMAPTPIPTRSPSDSSTASTPVAEQIERALDEVTSSQPPPLPPPPPPAQETQEPSPILDSEETRAAAPQAWAGPMEEPPQAQAPPRGPGSPAEDLMFMESAEQAVLAAPVEPATADATEIHDAADTIETDTATADTTVANNVPPAATSLIDLWPGNGEGASTLQGEPRAPTPPSGTEVTLAEVPLLDEVAPEPLLPAGEGCATLLNFDELPEPPATFCDPEEVEGESLAAPQTPTLPSALEELEQEQEPEPHLLTNGETTQKEGTQASEGYFSQSQEEEFAQSEELCAKAPPPVFYNKPPEIDITCWDADPVPEEEEGFEGGD A7DF1AE3776C0BEA dbn1-cxcr4 2 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 1A cxcr4-dbn1 1 5 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component colocalization colocalization 1B/C HIV-1 viruses triggered the recruitment of drebrin to the CD4/CXCR4 capping areas, whereas in the absence of HIV virus drebrin displayed a diffuse subcortical pattern. cxcr4-dbn1-1 1 4 unspecified role unspecified role neutral component neutral component n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 3 unspecified role unspecified role neutral component neutral component colocalization colocalization 3B/C/D Drebrin-GFP and Dreb(1–366)-GFP both co-localize with CXCR4 in the absence of viral particles, whereas much lower co-localization was observed for Dreb(319–707)-GFP and CXCR4 (Fig. 3C). HIV-1 viruses triggered the recruitment of drebrin to the CD4/CXCR4 capping areas, whereas in the absence of HIV virus drebrin displayed a diffuse subcortical pattern.