MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it The unique N-terminal region of SRMS regulates enzymatic activity and phosphorylation of its novel substrate Dok1. The unique N-terminal region of SRMS regulates enzymatic activity and phosphorylation of its novel substrate Dok1. FEBS J. (1742-464X) 2013 Goel RK., Miah S., Black K., Kalra N., Dai C., Lukong KE. suggested by lperfetto in vitro In vitro pull down pull down experimental particp experimental participant identification experimental particp Goel RK., Miah S., Black K., Kalra N., Dai C., Lukong KE. suggested by lperfetto FEBS J. (1742-464X) 2013 The unique N-terminal region of SRMS regulates enzymatic activity and phosphorylation of its novel substrate Dok1. The unique N-terminal region of SRMS regulates enzymatic activity and phosphorylation of its novel substrate Dok1. FEBS J. (1742-464X) 2013 Goel RK., Miah S., Black K., Kalra N., Dai C., Lukong KE. suggested by lperfetto human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip experimental particp experimental participant identification experimental particp Goel RK., Miah S., Black K., Kalra N., Dai C., Lukong KE. suggested by lperfetto FEBS J. (1742-464X) 2013 The unique N-terminal region of SRMS regulates enzymatic activity and phosphorylation of its novel substrate Dok1. The unique N-terminal region of SRMS regulates enzymatic activity and phosphorylation of its novel substrate Dok1. FEBS J. (1742-464X) 2013 Goel RK., Miah S., Black K., Kalra N., Dai C., Lukong KE. suggested by lperfetto human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip experimental particp experimental participant identification experimental particp Goel RK., Miah S., Black K., Kalra N., Dai C., Lukong KE. suggested by lperfetto FEBS J. (1742-464X) 2013 The unique N-terminal region of SRMS regulates enzymatic activity and phosphorylation of its novel substrate Dok1. The unique N-terminal region of SRMS regulates enzymatic activity and phosphorylation of its novel substrate Dok1. FEBS J. (1742-464X) 2013 Goel RK., Miah S., Black K., Kalra N., Dai C., Lukong KE. suggested by lperfetto in vitro In vitro protein kinase assay protein kinase assay anti tag western anti tag western blot anti tag western Goel RK., Miah S., Black K., Kalra N., Dai C., Lukong KE. suggested by lperfetto FEBS J. (1742-464X) 2013 dok1_human Docking protein 1 Downstream of tyrosine kinase 1 p62(dok) pp62 DOK1 protein protein human Homo sapiens Human MDGAVMEGPLFLQSQRFGTKRWRKTWAVLYPASPHGVARLEFFDHKGSSSGGGRGSSRRLDCKVIRLAECVSVAPVTVETPPEPGATAFRLDTAQRSHLLAADAPSSAAWVQTLCRNAFPKGSWTLAPTDNPPKLSALEMLENSLYSPTWEGSQFWVTVQRTEAAERCGLHGSYVLRVEAERLTLLTVGAQSQILEPLLSWPYTLLRRYGRDKVMFSFEAGRRCPSGPGTFTFQTAQGNDIFQAVETAIHRQKAQGKAGQGHDVLRADSHEGEVAEGKLPSPPGPQELLDSPPALYAEPLDSLRIAPCPSQDSLYSDPLDSTSAQAGEGVQRKKPLYWDLYEHAQQQLLKAKLTDPKEDPIYDEPEGLAPVPPQGLYDLPREPKDAWWCQARVKEEGYELPYNPATDDYAVPPPRSTKPLLAPKPQGPAFPEPGTATGSGIKSHNSALYSQVQKSGASGSWDCGLSRVGTDKTGVKSEGST E9D947831244BA6C srms_human Tyrosine-protein kinase Srms SRMS C20orf148 protein protein human Homo sapiens Human MEPFLRRRLAFLSFFWDKIWPAGGEPDHGTPGSLDPNTDPVPTLPAEPCSPFPQLFLALYDFTARCGGELSVRRGDRLCALEEGGGYIFARRLSGQPSAGLVPITHVAKASPETLSDQPWYFSGVSRTQAQQLLLSPPNEPGAFLIRPSESSLGGYSLSVRAQAKVCHYRVSMAADGSLYLQKGRLFPGLEELLTYYKANWKLIQNPLLQPCMPQKAPRQDVWERPHSEFALGRKLGEGYFGEVWEGLWLGSLPVAIKVIKSANMKLTDLAKEIQTLKGLRHERLIRLHAVCSGGEPVYIVTELMRKGNLQAFLGTPEGRALRLPPLLGFACQVAEGMSYLEEQRVVHRDLAARNVLVDDGLACKVADFGLARLLKDDIYSPSSSSKIPVKWTAPEAANYRVFSQKSDVWSFGVLLHEVFTYGQCPYEGMTNHETLQQIMRGYRLPRPAACPAEVYVLMLECWRSSPEERPSFATLREKLHAIHRCHP mint homomint 83193AD26C5F6F68 dok1-srms 4 SRMS 6 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp enzyme enzyme neutral component neutral component purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Dok-1 5 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp enzyme target enzyme target substrate substrate neutral component neutral component purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined phosphorylation phosphorylation reaction phosphorylation homomint mint f7f dok1-srms-1 3 SRMS 6 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Dok-1 5 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association homomint mint f6b f7a srms-dok1 3 SRMS 6 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Dok-1 5 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f6b homomint mint dok1-srms-2 3 SRMS 6 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Dok-1 5 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f7b homomint mint srms-dok1-1 1 SRMS 6 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Dok-1 5 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f6d homomint domino mint srms-dok1-2 1 SRMS 6 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Dok-1 5 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f6e homomint domino mint Tyrosine phosphorylated endogenous Dok1 from GFP-SRMStransfected HEK 293 cell lysates, binds to GST-SH2 domain of SRMS, shown via immunoblotting with anti-Dok1 dok1-srms-3 2 SRMS 6 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Dok-1 5 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association f7e homomint mint Furthermore, that co-immunoprecipitation of ectopic SRMS progressively diminished with the smaller Dok1 mutants (Fig 7E, bottom panel, lanes 1-6), indicates the underlying significance of the C-terminal proline residues in mediating binding interactions with SRMS. These data collectively provide strong evidence that Dok1 is a potential substrate of SRMS.