MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Dimerization of the CENP-A assembly factor HJURP is required for centromeric nucleosome deposition. Dimerization of the CENP-A assembly factor HJURP is required for centromeric nucleosome deposition. EMBO J. (0261-4189) 2013 imex curation Zasadzińska E., Barnhart-Dailey M.C., Kuich P.H., Foltz D.R. dfoltz@virginia.edu 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference complementation protein complementation assay PCA complementation tag fluorescence tag visualisation by fluorescence tag fluorescence Zasadzińska E., Barnhart-Dailey M.C., Kuich P.H., Foltz D.R. dfoltz@virginia.edu Accepted 2013-NOV-21 by MARTA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation Dimerization of the CENP-A assembly factor HJURP is required for centromeric nucleosome deposition. Dimerization of the CENP-A assembly factor HJURP is required for centromeric nucleosome deposition. EMBO J. (0261-4189) 2013 imex curation Zasadzińska E., Barnhart-Dailey M.C., Kuich P.H., Foltz D.R. dfoltz@virginia.edu 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zasadzińska E., Barnhart-Dailey M.C., Kuich P.H., Foltz D.R. dfoltz@virginia.edu Accepted 2013-NOV-21 by MARTA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation Dimerization of the CENP-A assembly factor HJURP is required for centromeric nucleosome deposition. Dimerization of the CENP-A assembly factor HJURP is required for centromeric nucleosome deposition. EMBO J. (0261-4189) 2013 imex curation Zasadzińska E., Barnhart-Dailey M.C., Kuich P.H., Foltz D.R. dfoltz@virginia.edu 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column anti tag western anti tag western blot anti tag western Zasadzińska E., Barnhart-Dailey M.C., Kuich P.H., Foltz D.R. dfoltz@virginia.edu Accepted 2013-NOV-21 by MARTA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation Dimerization of the CENP-A assembly factor HJURP is required for centromeric nucleosome deposition. Dimerization of the CENP-A assembly factor HJURP is required for centromeric nucleosome deposition. EMBO J. (0261-4189) 2013 imex curation Zasadzińska E., Barnhart-Dailey M.C., Kuich P.H., Foltz D.R. dfoltz@virginia.edu 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zasadzińska E., Barnhart-Dailey M.C., Kuich P.H., Foltz D.R. dfoltz@virginia.edu Accepted 2013-NOV-21 by MARTA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation hjurp_human Holliday junction recognition protein FAKTS URLC9 14-3-3-associated AKT substrate Fetal liver-expressing gene 1 protein Up-regulated in lung cancer 9 HJURP FLEG1 protein protein human Homo sapiens Human MLGTLRAMEGEDVEDDQLLQKLRASRRRFQRRMQRLIEKYNQPFEDTPVVQMATLTYETPQGLRIWGGRLIKERNEGEIQDSSMKPADRTDGSVQAAAWGPELPSHRTVLGADSKSGEVDATSDQEESVAWALAPAVPQSPLKNELRRKYLTQVDILLQGAEYFECAGNRAGRDVRVTPLPSLASPAVPAPGYCSRISRKSPGDPAKPASSPREWDPLHPSSTDMALVPRNDSLSLQETSSSSFLSSQPFEDDDICNVTISDLYAGMLHSMSRLLSTKPSSIISTKTFIMQNWNSRRRHRYKSRMNKTYCKGARRSQRSSKENFIPCSEPVKGTGALRDCKNVLDVSCRKTGLKLEKAFLEVNRPQIHKLDPSWKERKVTPSKYSSLIYFDSSATYNLDEENRFRTLKWLISPVKIVSRPTIRQGHGENRQREIEIRFDQLHREYCLSPRNQPRRMCLPDSWAMNMYRGGPASPGGLQGLETRRLSLPSSKAKAKSLSEAFENLGKRSLEAGRCLPKSDSSSSLPKTNPTHSATRPQQTSDLHVQGNSSGIFRKSVSPSKTLSVPDKEVPGHGRNRYDEIKEEFDKLHQKYCLKSPGQMTVPLCIGVSTDKASMEVRYQTEGFLGKLNPDPHFQGFQKLPSSPLGCRKSLLGSTAIEAPSSTCVARAITRDGTRDHQFPAKRPRLSEPQGSGRQGNSLGASDGVDNTVRPGDQGSSSQPNSEERGENTSYRMEEKSDFMLEKLETKSV DIP protein Q8NCD3 original sequence version: 56 DIP protein Q8NCD3 original sequence version: 104 DE7F1410F9A748D5 hjurp-hjurp-1 1 5 tag fluorescence tag visualisation by fluorescence tag fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell binding site sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain tag fusion protein fusion protein predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 tag fluorescence tag visualisation by fluorescence tag fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association f2b f2c f2d f2e hjurp-1 3 5 anti tag western anti tag western blot anti tag western anti tag western anti tag western blot anti tag western unspecified role unspecified role unspecified role unspecified role purified purified binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 anti tag western anti tag western blot anti tag western anti tag western anti tag western blot anti tag western unspecified role unspecified role unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction f3b f3c sf1b Based on size-exclusion chromatography, the Stokes radius of His-HJURP482-end was calculated as 5.46nm (Figure 3B and C; Supplementary Figure S1B), which is twice as large as the expected 2.48nm Stokes radius of a 30-kDa globular protein. This larger than expected Stokes radius may indicate either that HJURP482-end is an elongated protein or that it exists as a multimer. hjurp-hjurp-2 2 5 anti tag western anti tag western blot anti tag western anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 anti tag western anti tag western blot anti tag western anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction f3e hjurp-hjurp-3 4 5 anti tag western anti tag western blot anti tag western anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell binding site sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 anti tag western anti tag western blot anti tag western anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f4c