IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Amyloid precursor proteins interact with the heterotrimeric G protein Go in the control of neuronal migration. Amyloid precursor proteins interact with the heterotrimeric G protein Go in the control of neuronal migration. J. Neurosci. (0270-6474) 2013 imex curation Ramaker J.M., Swanson T.L., Copenhaver P.F. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease Alzheimers - Interactions investigated in the context of Alzheimers disease EMAIL sent 31/10/13 moth APPL= Q4ZHV6 and P53359 = (GNAO_MANSE Blasting in FlyBase (FlyBlast??!) the given antibody immunogen sequence (is mammalian) for Calbiochem #371726 (used here to detect Gi/Goa) does not give any results for Drome - neither does Expasy blast. The relevant Drome Goa protein is I think is P16378 - which has no sequence > 4 aas having homology with the immunogen sequence, so it is anyones guess what it is detecting in fig 3F etc...Antibody target for "anti-Gβγ" could be many proteins (a complex). Results from Drosophila and some Mouse IPs cannot be included as the antibodies used are either do not have a specific target and/or target identity is unknown. copenhav@ohsu.edu 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions drome-larval _immaginal_disc Drosophila melanogaster larva_ imaginal_disc larval imaginal disc bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Ramaker J.M., Swanson T.L., Copenhaver P.F. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. EMAIL sent 31/10/13 moth APPL= Q4ZHV6 and P53359 = (GNAO_MANSE Blasting in FlyBase (FlyBlast??!) the given antibody immunogen sequence (is mammalian) for Calbiochem #371726 (used here to detect Gi/Goa) does not give any results for Drome - neither does Expasy blast. The relevant Drome Goa protein is I think is P16378 - which has no sequence > 4 aas having homology with the immunogen sequence, so it is anyones guess what it is detecting in fig 3F etc...Antibody target for "anti-Gβγ" could be many proteins (a complex). Results from Drosophila and some Mouse IPs cannot be included as the antibodies used are either do not have a specific target and/or target identity is unknown. copenhav@ohsu.edu Accepted 2013-DEC-12 by PPORRAS J. Neurosci. (0270-6474) 2013 Only protein-protein interactions imex curation Amyloid precursor proteins interact with the heterotrimeric G protein Go in the control of neuronal migration. Amyloid precursor proteins interact with the heterotrimeric G protein Go in the control of neuronal migration. J. Neurosci. (0270-6474) 2013 imex curation Ramaker J.M., Swanson T.L., Copenhaver P.F. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease Alzheimers - Interactions investigated in the context of Alzheimers disease EMAIL sent 31/10/13 moth APPL= Q4ZHV6 and P53359 = (GNAO_MANSE Blasting in FlyBase (FlyBlast??!) the given antibody immunogen sequence (is mammalian) for Calbiochem #371726 (used here to detect Gi/Goa) does not give any results for Drome - neither does Expasy blast. The relevant Drome Goa protein is I think is P16378 - which has no sequence > 4 aas having homology with the immunogen sequence, so it is anyones guess what it is detecting in fig 3F etc...Antibody target for "anti-Gβγ" could be many proteins (a complex). Results from Drosophila and some Mouse IPs cannot be included as the antibodies used are either do not have a specific target and/or target identity is unknown. copenhav@ohsu.edu 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ramaker J.M., Swanson T.L., Copenhaver P.F. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. EMAIL sent 31/10/13 moth APPL= Q4ZHV6 and P53359 = (GNAO_MANSE Blasting in FlyBase (FlyBlast??!) the given antibody immunogen sequence (is mammalian) for Calbiochem #371726 (used here to detect Gi/Goa) does not give any results for Drome - neither does Expasy blast. The relevant Drome Goa protein is I think is P16378 - which has no sequence > 4 aas having homology with the immunogen sequence, so it is anyones guess what it is detecting in fig 3F etc...Antibody target for "anti-Gβγ" could be many proteins (a complex). Results from Drosophila and some Mouse IPs cannot be included as the antibodies used are either do not have a specific target and/or target identity is unknown. copenhav@ohsu.edu Accepted 2013-DEC-12 by PPORRAS J. Neurosci. (0270-6474) 2013 Only protein-protein interactions imex curation Amyloid precursor proteins interact with the heterotrimeric G protein Go in the control of neuronal migration. Amyloid precursor proteins interact with the heterotrimeric G protein Go in the control of neuronal migration. J. Neurosci. (0270-6474) 2013 imex curation Ramaker J.M., Swanson T.L., Copenhaver P.F. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease Alzheimers - Interactions investigated in the context of Alzheimers disease EMAIL sent 31/10/13 moth APPL= Q4ZHV6 and P53359 = (GNAO_MANSE Blasting in FlyBase (FlyBlast??!) the given antibody immunogen sequence (is mammalian) for Calbiochem #371726 (used here to detect Gi/Goa) does not give any results for Drome - neither does Expasy blast. The relevant Drome Goa protein is I think is P16378 - which has no sequence > 4 aas having homology with the immunogen sequence, so it is anyones guess what it is detecting in fig 3F etc...Antibody target for "anti-Gβγ" could be many proteins (a complex). Results from Drosophila and some Mouse IPs cannot be included as the antibodies used are either do not have a specific target and/or target identity is unknown. copenhav@ohsu.edu 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Ramaker J.M., Swanson T.L., Copenhaver P.F. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. EMAIL sent 31/10/13 moth APPL= Q4ZHV6 and P53359 = (GNAO_MANSE Blasting in FlyBase (FlyBlast??!) the given antibody immunogen sequence (is mammalian) for Calbiochem #371726 (used here to detect Gi/Goa) does not give any results for Drome - neither does Expasy blast. The relevant Drome Goa protein is I think is P16378 - which has no sequence > 4 aas having homology with the immunogen sequence, so it is anyones guess what it is detecting in fig 3F etc...Antibody target for "anti-Gβγ" could be many proteins (a complex). Results from Drosophila and some Mouse IPs cannot be included as the antibodies used are either do not have a specific target and/or target identity is unknown. copenhav@ohsu.edu Accepted 2013-DEC-12 by PPORRAS J. Neurosci. (0270-6474) 2013 Only protein-protein interactions imex curation Amyloid precursor proteins interact with the heterotrimeric G protein Go in the control of neuronal migration. Amyloid precursor proteins interact with the heterotrimeric G protein Go in the control of neuronal migration. J. Neurosci. (0270-6474) 2013 imex curation Ramaker J.M., Swanson T.L., Copenhaver P.F. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease Alzheimers - Interactions investigated in the context of Alzheimers disease EMAIL sent 31/10/13 moth APPL= Q4ZHV6 and P53359 = (GNAO_MANSE Blasting in FlyBase (FlyBlast??!) the given antibody immunogen sequence (is mammalian) for Calbiochem #371726 (used here to detect Gi/Goa) does not give any results for Drome - neither does Expasy blast. The relevant Drome Goa protein is I think is P16378 - which has no sequence > 4 aas having homology with the immunogen sequence, so it is anyones guess what it is detecting in fig 3F etc...Antibody target for "anti-Gβγ" could be many proteins (a complex). Results from Drosophila and some Mouse IPs cannot be included as the antibodies used are either do not have a specific target and/or target identity is unknown. copenhav@ohsu.edu 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions manse-gv1 Manduca sexta GV1 cell line gv1 Manduca GV1 cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ramaker J.M., Swanson T.L., Copenhaver P.F. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. EMAIL sent 31/10/13 moth APPL= Q4ZHV6 and P53359 = (GNAO_MANSE Blasting in FlyBase (FlyBlast??!) the given antibody immunogen sequence (is mammalian) for Calbiochem #371726 (used here to detect Gi/Goa) does not give any results for Drome - neither does Expasy blast. The relevant Drome Goa protein is I think is P16378 - which has no sequence > 4 aas having homology with the immunogen sequence, so it is anyones guess what it is detecting in fig 3F etc...Antibody target for "anti-Gβγ" could be many proteins (a complex). Results from Drosophila and some Mouse IPs cannot be included as the antibodies used are either do not have a specific target and/or target identity is unknown. copenhav@ohsu.edu Accepted 2013-DEC-12 by PPORRAS J. Neurosci. (0270-6474) 2013 Only protein-protein interactions imex curation Amyloid precursor proteins interact with the heterotrimeric G protein Go in the control of neuronal migration. Amyloid precursor proteins interact with the heterotrimeric G protein Go in the control of neuronal migration. J. Neurosci. (0270-6474) 2013 imex curation Ramaker J.M., Swanson T.L., Copenhaver P.F. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease Alzheimers - Interactions investigated in the context of Alzheimers disease EMAIL sent 31/10/13 moth APPL= Q4ZHV6 and P53359 = (GNAO_MANSE Blasting in FlyBase (FlyBlast??!) the given antibody immunogen sequence (is mammalian) for Calbiochem #371726 (used here to detect Gi/Goa) does not give any results for Drome - neither does Expasy blast. The relevant Drome Goa protein is I think is P16378 - which has no sequence > 4 aas having homology with the immunogen sequence, so it is anyones guess what it is detecting in fig 3F etc...Antibody target for "anti-Gβγ" could be many proteins (a complex). Results from Drosophila and some Mouse IPs cannot be included as the antibodies used are either do not have a specific target and/or target identity is unknown. copenhav@ohsu.edu 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions rat-neuron Rattus norvegicus neuronal cell neuron Neuron [Neuronal cell; Neural cell confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Ramaker J.M., Swanson T.L., Copenhaver P.F. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. EMAIL sent 31/10/13 moth APPL= Q4ZHV6 and P53359 = (GNAO_MANSE Blasting in FlyBase (FlyBlast??!) the given antibody immunogen sequence (is mammalian) for Calbiochem #371726 (used here to detect Gi/Goa) does not give any results for Drome - neither does Expasy blast. The relevant Drome Goa protein is I think is P16378 - which has no sequence > 4 aas having homology with the immunogen sequence, so it is anyones guess what it is detecting in fig 3F etc...Antibody target for "anti-Gβγ" could be many proteins (a complex). hippocampal neurons Results from Drosophila and some Mouse IPs cannot be included as the antibodies used are either do not have a specific target and/or target identity is unknown. copenhav@ohsu.edu Accepted 2013-DEC-12 by PPORRAS J. Neurosci. (0270-6474) 2013 Only protein-protein interactions imex curation Amyloid precursor proteins interact with the heterotrimeric G protein Go in the control of neuronal migration. Amyloid precursor proteins interact with the heterotrimeric G protein Go in the control of neuronal migration. J. Neurosci. (0270-6474) 2013 imex curation Ramaker J.M., Swanson T.L., Copenhaver P.F. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease Alzheimers - Interactions investigated in the context of Alzheimers disease EMAIL sent 31/10/13 moth APPL= Q4ZHV6 and P53359 = (GNAO_MANSE Blasting in FlyBase (FlyBlast??!) the given antibody immunogen sequence (is mammalian) for Calbiochem #371726 (used here to detect Gi/Goa) does not give any results for Drome - neither does Expasy blast. The relevant Drome Goa protein is I think is P16378 - which has no sequence > 4 aas having homology with the immunogen sequence, so it is anyones guess what it is detecting in fig 3F etc...Antibody target for "anti-Gβγ" could be many proteins (a complex). Results from Drosophila and some Mouse IPs cannot be included as the antibodies used are either do not have a specific target and/or target identity is unknown. copenhav@ohsu.edu 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions manse-embryo manse embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ramaker J.M., Swanson T.L., Copenhaver P.F. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. EMAIL sent 31/10/13 moth APPL= Q4ZHV6 and P53359 = (GNAO_MANSE Blasting in FlyBase (FlyBlast??!) the given antibody immunogen sequence (is mammalian) for Calbiochem #371726 (used here to detect Gi/Goa) does not give any results for Drome - neither does Expasy blast. The relevant Drome Goa protein is I think is P16378 - which has no sequence > 4 aas having homology with the immunogen sequence, so it is anyones guess what it is detecting in fig 3F etc...Antibody target for "anti-Gβγ" could be many proteins (a complex). Results from Drosophila and some Mouse IPs cannot be included as the antibodies used are either do not have a specific target and/or target identity is unknown. copenhav@ohsu.edu Accepted 2013-DEC-12 by PPORRAS J. Neurosci. (0270-6474) 2013 Only protein-protein interactions imex curation Amyloid precursor proteins interact with the heterotrimeric G protein Go in the control of neuronal migration. Amyloid precursor proteins interact with the heterotrimeric G protein Go in the control of neuronal migration. J. Neurosci. (0270-6474) 2013 imex curation Ramaker J.M., Swanson T.L., Copenhaver P.F. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease Alzheimers - Interactions investigated in the context of Alzheimers disease EMAIL sent 31/10/13 moth APPL= Q4ZHV6 and P53359 = (GNAO_MANSE Blasting in FlyBase (FlyBlast??!) the given antibody immunogen sequence (is mammalian) for Calbiochem #371726 (used here to detect Gi/Goa) does not give any results for Drome - neither does Expasy blast. The relevant Drome Goa protein is I think is P16378 - which has no sequence > 4 aas having homology with the immunogen sequence, so it is anyones guess what it is detecting in fig 3F etc...Antibody target for "anti-Gβγ" could be many proteins (a complex). Results from Drosophila and some Mouse IPs cannot be included as the antibodies used are either do not have a specific target and/or target identity is unknown. copenhav@ohsu.edu 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions manse-neuron Manduca sexta Neuron neuron Neuron [Neuronal cell; Neural cell confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Ramaker J.M., Swanson T.L., Copenhaver P.F. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. EMAIL sent 31/10/13 moth APPL= Q4ZHV6 and P53359 = (GNAO_MANSE Blasting in FlyBase (FlyBlast??!) the given antibody immunogen sequence (is mammalian) for Calbiochem #371726 (used here to detect Gi/Goa) does not give any results for Drome - neither does Expasy blast. The relevant Drome Goa protein is I think is P16378 - which has no sequence > 4 aas having homology with the immunogen sequence, so it is anyones guess what it is detecting in fig 3F etc...Antibody target for "anti-Gβγ" could be many proteins (a complex). EP cells:- a population of ∼300 neurons (EP cells) that undergoes a stereotyped sequence of migration along preformed pathways to form a branching nerve plexus (the EP). Results from Drosophila and some Mouse IPs cannot be included as the antibodies used are either do not have a specific target and/or target identity is unknown. copenhav@ohsu.edu Accepted 2013-DEC-12 by PPORRAS J. Neurosci. (0270-6474) 2013 Only protein-protein interactions imex curation Amyloid precursor proteins interact with the heterotrimeric G protein Go in the control of neuronal migration. Amyloid precursor proteins interact with the heterotrimeric G protein Go in the control of neuronal migration. J. Neurosci. (0270-6474) 2013 imex curation Ramaker J.M., Swanson T.L., Copenhaver P.F. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease Alzheimers - Interactions investigated in the context of Alzheimers disease EMAIL sent 31/10/13 moth APPL= Q4ZHV6 and P53359 = (GNAO_MANSE Blasting in FlyBase (FlyBlast??!) the given antibody immunogen sequence (is mammalian) for Calbiochem #371726 (used here to detect Gi/Goa) does not give any results for Drome - neither does Expasy blast. The relevant Drome Goa protein is I think is P16378 - which has no sequence > 4 aas having homology with the immunogen sequence, so it is anyones guess what it is detecting in fig 3F etc...Antibody target for "anti-Gβγ" could be many proteins (a complex). Results from Drosophila and some Mouse IPs cannot be included as the antibodies used are either do not have a specific target and/or target identity is unknown. copenhav@ohsu.edu 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions human-brain-cortex Homo sapiens brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ramaker J.M., Swanson T.L., Copenhaver P.F. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. EMAIL sent 31/10/13 moth APPL= Q4ZHV6 and P53359 = (GNAO_MANSE Blasting in FlyBase (FlyBlast??!) the given antibody immunogen sequence (is mammalian) for Calbiochem #371726 (used here to detect Gi/Goa) does not give any results for Drome - neither does Expasy blast. The relevant Drome Goa protein is I think is P16378 - which has no sequence > 4 aas having homology with the immunogen sequence, so it is anyones guess what it is detecting in fig 3F etc...Antibody target for "anti-Gβγ" could be many proteins (a complex). Human brain lysates prepared from the cortical regions of healthy control subjects. Results from Drosophila and some Mouse IPs cannot be included as the antibodies used are either do not have a specific target and/or target identity is unknown. copenhav@ohsu.edu Accepted 2013-DEC-12 by PPORRAS J. Neurosci. (0270-6474) 2013 Only protein-protein interactions imex curation q4zhv6_manse protein protein manse Manduca sexta (Tobacco hawkmoth) Tobacco hornworm Tobacco hawkmoth MTRAVLFISVFTIFLDVLHAGQASSGAEPQVAVLCEAGSTYHPQYMSAAGRWTPDLTTKPHNCLKDKMEILDYCKKVYPSHDITNIVEASHYVKVSNWCKLGTSNAAKCKVTRWVKPFRCLEGPFQSDALLVPESCLFDHIHNQSRCWQFARWNATAGRACAQRGLRLRTFAMLLPCGISLFSGVEFVCCPKHFKENVKMHKPMDVGVPVSPGGEDMLAASAAMDERDDDLLDDDDALDDDDDDDTLNLSDDDDDDDADDDMDEDEDADLSRDDDAEDDDYTDADDSAWPRPESSSSPSTTTSTTTTTTTTTASTATSDPYFSHFDPRTEHQSYKDAQQRLEETHREKITKVMKEWSELEDRYQAMMSADPAAAQTFRQRMTAKFQANVQSLEEEGVAERRRLAALHQQRVLAHLAQRRRTALACYTRSLRDTPPNAHRVQKCLQRLVRALAAERSGALAAWRRAAAAGREAAAAERTSAADRLQDADRALQRALTALRRRPHLYASIGTAIEDYVQSMQSKDDMAVSLMSMTPEAEELLLDRIEAEVQREQAAREQLSVKRDQRARQRQDIQNERAKTSNGVKESEESDDEASEPSENETSTAAPTPTSAPSSPASATPAPSAAPVSVHDITTRSGFTQDTTTTEMITTTVTDAPESETAETIEASETTSRRSTSETEGEGLRAALEHAEERAPPPPAHALKHELQHSQPGYTVRGAGPSGAGGAGGSGALYPALCVGGAALAAAACVALAVARRRDRAPHAQGFVQVEQTGVVAPTPEERHVANMQINGYENPTYKYFEVKE C7222A7D39F73E57 gnao_rat Guanine nucleotide-binding protein G(o) subunit alpha Gnao1 Gna0 Gnao protein protein rat Rattus norvegicus (Rat) Rat MGCTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGAGESGKSTIVKQMKIIHEDGFSGEDVKQYKPVVYSNTIQSLAAIVRAMDTLGVEYGDKERKADSKMVCDVVSRMEDTEPFSAELLSAMMRLWGDSGIQECFNRSREYQLNDSAKYYLDSLDRIGAADYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIFCVALSGYDQVLHEDETTNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPLTICFPEYPGSNTYEDAAAYIQTQFESKNRSPNKEIYCHMTCATDTNNIQVVFDAVTDIIIANNLRGCGLY DIP protein P59215 original sequence version: 119 DIP protein P59215 original sequence version: 86 mint Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear homomint 577024F61B179C89 a4_human Amyloid-beta precursor protein APP A4 AD1 Alzheimer disease amyloid protein ABPP PreA4 Cerebral vascular amyloid peptide Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAMSQSLLKTTQEPLARDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN DIP protein P05067 original sequence version: 160 DIP protein P05067 original sequence version: 207 DIP protein P05067 original sequence version: 185 DIP protein P05067 original sequence version: 188 A12EE761403740F5 gnao_human Guanine nucleotide-binding protein G(o) subunit alpha GNAO1 protein protein human Homo sapiens Human MGCTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGAGESGKSTIVKQMKIIHEDGFSGEDVKQYKPVVYSNTIQSLAAIVRAMDTLGIEYGDKERKADAKMVCDVVSRMEDTEPFSAELLSAMMRLWGDSGIQECFNRSREYQLNDSAKYYLDSLDRIGAADYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIFCVALSGYDQVLHEDETTNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPLTICFPEYTGPNTYEDAAAYIQAQFESKNRSPNKEIYCHMTCATDTNNIQVVFDAVTDIIIANNLRGCGLY 4F182926A8106E3E gnai_drome G protein alpha i subunit Galphai G-OA65C G-ialpha65A Guanine nucleotide-binding protein G(i) subunit alpha 65A CG10060 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGCAVSTARDKEAIERSKNIDRALRAEGERAASEVKLLLLGAGESGKSTIVKQMKIIHDTGYSQEECEEYRRVVFSNTVQSLMVIIRAMGRLKIEFADPSRTDIARQFFTHASAADEGILLPEIVLLMKKLWADGGVQQSFARSREYQLNDSAGYYLNSLDRIAQPNYIPTQQDVLRTRVKTTGIIETHFSCKQLHFKLFDVGGQRSERKKWIHCFEGVTAIIFCVALSGYDLVLAEDEEMNRMIESLKLFDSICNSKWFVETSIILFLNKKDLFEEKIKRSPLTICFPEYTGTNTFEEAANYIRMKFENLNKRKDQKEIYTHLTCATDTNNVKFVFDAVTDVIIKNNLKQIGLF DIP protein P20353 original sequence version: 124 69DF15754EB22F9D a4_rat Amyloid-beta precursor protein Alzheimer disease amyloid A4 protein homolog ABPP Amyloidogenic glycoprotein App Amyloid precursor protein A4 AD1 Alzheimer disease amyloid protein Amyloid-beta (A4) precursor protein Amyloid-beta A4 protein protein protein rat Rattus norvegicus (Rat) Rat MLPSLALLLLAAWTVRALEVPTDGNAGLLAEPQIAMFCGKLNMHMNVQNGKWESDPSGTKTCIGTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHTHIVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDSIDSADAEEDDSDVWWGGADTDYADGGEDKVVEVAEEEEVADVEEEEAEDDEDVEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSVSSQSLLKTTSEPLPQDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPHHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHPFGVDSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFGHDSGFEVRHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN DIP protein P08592 original sequence version: 136 C26C9D6BB2D929A7 gnao_mouse Guanine nucleotide-binding protein G(o) subunit alpha Gnao1 Gna0 Gnao protein protein mouse Mus musculus Mouse MGCTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGAGESGKSTIVKQMKIIHEDGFSGEDVKQYKPVVYSNTIQSLAAIVRAMDTLGVEYGDKERKTDSKMVCDVVSRMEDTEPFSAELLSAMMRLWGDSGIQECFNRSREYQLNDSAKYYLDSLDRIGAGDYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIFCVALSGYDQVLHEDETTNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPLTICFPEYPGSNTYEDAAAYIQTQFESKNRSPNKEIYCHMTCATDTNNIQVVFDAVTDIIIANNLRGCGLY B73A84F3BDB09F2C gnao_manse Guanine nucleotide-binding protein G(o) subunit alpha protein protein manse Manduca sexta (Tobacco hawkmoth) Tobacco hornworm Tobacco hawkmoth MGCASSAEERAAPSAQQADREKLKEDGIQAAKDIKLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTIQSLVAILRAMPTLGITYANKDRESDGKMVFDVIQRMEDTEPFSEELLAAMKRLWADAGVQECFGRSNEYQLNDSAKYFLDDLDRLGARDYQPTEEDILRTRVKTTGIVEVHFSFKNLNFKLFDVGRGQRSERKKWIHCFEDVTAIIFCVAMSEYDQVLHEDETTNRMQESLKLFDSICNNKWFTDTSIILFLNKKDLFEEKIRKSPLTICFPEYTRAYQEYGEAAAYIRSQFEAKNNSTTKEIYCHMTATDTNNNQFVFDAVTDVIIANNLRGCGLY 593C96EA069AFF48 a4_mouse Amyloid-beta precursor protein App Alzheimer disease amyloid A4 protein homolog ABPP Amyloidogenic glycoprotein Amyloid precursor protein A4 AD1 Alzheimer disease amyloid protein Amyloid-beta (A4) precursor protein Amyloid-beta A4 protein protein protein mouse Mus musculus Mouse MLPSLALLLLAAWTVRALEVPTDGNAGLLAEPQIAMFCGKLNMHMNVQNGKWESDPSGTKTCIGTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHTHIVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDSVDSADAEEDDSDVWWGGADTDYADGGEDKVVEVAEEEEVADVEEEEADDDEDVEDGDEVEEEAEEPYEEATERTTSTATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCVPFFYGGCGGNRNNFDTEEYCMAVCGSVSTQSLLKTTSEPLPQDPDKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPHHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHPFGVDSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFGHDSGFEVRHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN 988D89E089092A3E gnao-q4zhv6 7 9 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component colocalization colocalization Figure 2 K-Q app-gnao1 5 10 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component colocalization colocalization 2 R-T q4zhv6-gnao 6 9 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey physical association physical association Figure 3 B/C, 6C/D Endogenously expressed Goα could be readily coimmunoprecipitated with both N-terminal- and C-terminal-specific APPL antibodies. GTPgammaS- - a nonhydrolyzable GTP analog that activates all G proteins. Mas 7 (a potent analog of Mastoparan, a peptide toxin from wasp venom) PTX -Pertussis Toxin (a specific inhibitor of Goα in insects) GDPbetaS- an inhibitor of G protein activation. app-gnao1-1 2 17 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey physical association physical association 3H app-gnao1-2 8 12 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association 3K gnao-q4zhv6-1 3 16 unspecified role unspecified role neutral component neutral component n_terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role neutral component neutral component region venus-n venus n-terminal fragment undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined go_binding_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 4C/D/E gnao-q4zhv6-2 1 16 unspecified role unspecified role neutral component neutral component n_terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role neutral component neutral component region venus-n venus n-terminal fragment undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined go_binding_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 5 q4zhv6-gnao-1 4 9 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey physical association physical association Figure 6B GTPgammaS - a nonhydrolyzable GTP analog that activates all G proteins. GDPbetaS - an inhibitor of G protein activation. gnao-q4zhv6-3 3 13 unspecified role unspecified role neutral component neutral component n_terminus venus-c venus c-terminal fragment n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role neutral component neutral component region venus-n venus n-terminal fragment undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined go_binding_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 4F3 Cotransfection of APPL-Vn1 with Vn2-Giα produced only faint BiFC signals that were predominantly restricted to Golgi/ER regions (Fig. 4F3).